Name: Alkalihalobacillus alkalilacus AK73
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158725

Type strain:

Species: Alkalihalobacillus alkalilacus

Strain Designation: AK73

Culture col. no.: JCM 32184, MTCC 12637, KCTC 33880

Strain history: P. Anil Kumar; MTCC, India; AK73.

NCBI tax ID(s): 1983720 (species)

Section

Alkalihalobacillus alkalilacus AK73 is an aerobe, chemoorganotroph, spore-forming bacterium that was isolated from a sediment sample collected from the haloalkaline Sambhar lake, Jaipur.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
chemoorganotroph
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Alkalihalobacillus*
	Species	**Alkalihalobacillus alkalilacus**
	Full Scientific Name (LPSN)	Alkalihalobacillus alkalilacus (Singh et al. 2018) Gupta et al. 2020

	Synonym	"Shouchella* alkalilacus"*
	Synonym	Bacillus alkalilacus

Information on morphological and physiological properties Morphology

[Ref.: #65472]	Gram stain	positive

[Ref.: #65472]	Cell length	2.5-3.3 µm

[Ref.: #65472]	Cell width	0.7-0.9 µm

[Ref.: #65472]	Cell shape	rod-shaped

[Ref.: #65472]	Motility	yes

[Ref.: #65472]	Colony size	1-2 mm
[Ref.: #65472]	Colony color	yellowish
[Ref.: #65472]	Incubation period	2 days
[Ref.: #65472]	Cultivation medium used	marine agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65472]

Culture medium

Marine agar (MA)

[Ref.: #65472]

Culture medium growth

		Temperature
[Ref.: #65472]	growth	15-42 °C
[Ref.: #65472]	optimum	30-37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #65472]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65472]			optimum	9
[Ref.: #65472]			growth	7-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65472]

Oxygen tolerance

aerobe

[Ref.: #65472]

Nutrition type

chemoorganotroph

[Ref.: #65472]	Spore description	central endospores
[Ref.: #65472]	Type of spore	endospore
[Ref.: #65472]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65472]		NaCl		growth	0-10	%(w/v)
[Ref.: #65472]		NaCl		optimum	2	%(w/v)

[Ref.: #65472]	Observation	divide by binary fission
[Ref.: #65472]	Observation	menaquinone 7 (MK-7) is the major respiratory quinone
[Ref.: #65472]	Observation	the cell-wall peptidoglycan contains meso-diaminopimelic acid
[Ref.: #65472]	Observation	polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65472]	agar ChEBI	-	hydrolysis
[Ref.: #65472]	casein	-	hydrolysis
[Ref.: #65472]	esculin ChEBI	-	hydrolysis
[Ref.: #65472]	gelatin ChEBI	-	hydrolysis
[Ref.: #65472]	O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
[Ref.: #65472]	starch ChEBI	-	hydrolysis
[Ref.: #65472]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65472]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65472]	tween 60 ChEBI	-	hydrolysis
[Ref.: #65472]	tween 80 ChEBI	+	hydrolysis

	Metabolite production	Metabolite	Production
[Ref.: #65472]		hydrogen sulfide ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #65472]	acetoin ChEBI	-
[Ref.: #65472]	glucose ChEBI		-
[Ref.: #65472]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #65472]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65472]	arginine dihydrolase	+	3.5.3.6
[Ref.: #65472]	catalase	+	1.11.1.6
[Ref.: #65472]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65472]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65472]	nitrate reductase	-	1.7.99.4
[Ref.: #65472]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65472]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #65472]	pyrrolidonyl arylamidase	+	3.4.19.3
[Ref.: #65472]	tyrosine arylamidase	+
[Ref.: #65472]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65472]

Fatty acid	Percentage
C15:0 anteiso	23.9
C15:0 iso	13.9
C16:0	11.6
C17:0 anteiso	10.5
C16:0 iso	9.5
C14:0 iso	4.5
C16:1ω7c alcohol	3.8
C17:0 iso	3.2
C16:1ω11c	3.1
C18:0	3
C16:1ω7c and/or C16:1ω6c and/or iso-C1	2.7
C17:1 iso I and/or C17:1 anteiso B	2.6
C14:0	1.4
C18:1ω7c and/or C18:1ω6c	0.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65472]	Sample type/isolated from	a sediment sample collected from the haloalkaline Sambhar lake, Jaipur
[Ref.: #65472]	Geographic location (country and/or sea, region)	Rajasthan
[Ref.: #65472]	Country	India
[Ref.: #65472]	Country ISO 3 Code	IND
[Ref.: #65472]	Continent	Asia
[Ref.: #65472]	Geographic location	26.9253°/75.1970°
[Ref.: #65472]	Enrichment culture	Zobell's marine agar
[Ref.: #65472]	Enrichment culture duration	5 days
[Ref.: #65472]	Enrichment culture temperature	37 ̊C
[Ref.: #65472]	Isolation/enrichment procedure	sediment sample was serially diluted (up to 10-fold dilution) in 2 % (w/v) NaCl solution and 100 µl of each dilution was spread-plated on Zobell's marine agar (MA; HiMedia) plates

[Ref.: #67770]	Sample type/isolated from	Sediment from Sambhar lake
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jaipur, Rajasthan
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Alkaline	-
#Condition	#Saline	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bacillus sp. AK73 partial 16S rRNA gene, strain AK73, isolate SK 14A/4	LT844663	1455	ENA	1983720 tax ID

[Ref.: #65472]	GC-content	54 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	51.7-56.3 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65472]

Culture collection no.

JCM 32184, MTCC 12637, KCTC 33880

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65472

Harjodh Singh, Manpreet Kaur, Shivani Sharma, Lakhwinder Kaur, Sunita Mishra, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka: Bacillus alkalilacus sp. nov., isolated from a sediment sample from a lake in India. IJSEM 68: 1665 - 1671 2018 ( DOI 10.1099/ijsem.0.002726 , PubMed 29616894 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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