Name: Neptunicella marina S27-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158718

Type strain:

Species: Neptunicella marina

Strain Designation: S27-2

Culture col. no.: KCTC 52335, MCCC 1A02149

NCBI tax ID(s): 2125989 (species)

Section

Neptunicella marina S27-2 is an aerobe, motile, rod-shaped bacterium that forms circular colonies and was isolated from a surface seawater sample during cruise DY105-17A of the R/V Da-Yang Yi-Hao, at site IR-CTD13.

colony-forming
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	*Alteromonadaceae*
	Genus	*Neptunicella*
	Species	**Neptunicella marina**
	Full Scientific Name (LPSN)	Neptunicella marina Liu et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65462]	Gram stain	negative

[Ref.: #65462]	Cell length	1.0-2.0 µm

[Ref.: #65462]	Cell width	0.5-1 µm

[Ref.: #65462]	Cell shape	rod-shaped

[Ref.: #65462]	Motility	yes

[Ref.: #65462]	Flagellum arrangement	polar

[Ref.: #65462]	Colony color	creamy white
[Ref.: #65462]	Colony shape	circular
[Ref.: #65462]	Cultivation medium used	marine agar

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #65462]			growth	10-41 °C
[Ref.: #65462]			optimum	25-37 °C

[Ref.: #65462]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #65462]			optimum	7
[Ref.: #65462]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65462]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65462]		NaCl		growth	0-12	%
[Ref.: #65462]		NaCl		minimum	0.5-2	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65462]	adipate ChEBI	+	assimilation
[Ref.: #65462]	amygdalin ChEBI	+	fermentation
[Ref.: #65462]	arabinose ChEBI	-	fermentation
[Ref.: #65462]	casein	-
[Ref.: #65462]	D-glucose ChEBI	+	assimilation
[Ref.: #65462]	D-glucose ChEBI	+	fermentation
[Ref.: #65462]	D-mannitol ChEBI	+	assimilation
[Ref.: #65462]	D-mannose ChEBI	+	assimilation
[Ref.: #65462]	decanoate ChEBI	+	assimilation
[Ref.: #65462]	L-arabinose ChEBI	+	assimilation
[Ref.: #65462]	malate ChEBI	+	assimilation
[Ref.: #65462]	maltose ChEBI	+	assimilation
[Ref.: #65462]	mannitol ChEBI	-	fermentation
[Ref.: #65462]	melibiose ChEBI	-	fermentation
[Ref.: #65462]	myo-inositol ChEBI	-	fermentation
[Ref.: #65462]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65462]	nitrate ChEBI	-	reduction
[Ref.: #65462]	phenylacetate ChEBI	+	assimilation
[Ref.: #65462]	potassium gluconate ChEBI	+	assimilation
[Ref.: #65462]	rhamnose ChEBI	-	fermentation
[Ref.: #65462]	sodium citrate ChEBI	+	assimilation
[Ref.: #65462]	sorbitol ChEBI	-	fermentation
[Ref.: #65462]	starch ChEBI	-
[Ref.: #65462]	sucrose ChEBI	+	fermentation
[Ref.: #65462]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65462]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65462]	tween 60 ChEBI	+	hydrolysis
[Ref.: #65462]	tween 80 ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65462]	acetoin ChEBI	yes
[Ref.: #65462]	indole ChEBI	no
[Ref.: #65462]	sulfide ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #65462]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #65462]	acid phosphatase	+	3.1.3.2
[Ref.: #65462]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65462]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #65462]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65462]	alpha-galactosidase	+	3.2.1.22
[Ref.: #65462]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65462]	alpha-mannosidase	+	3.2.1.24
[Ref.: #65462]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65462]	beta-galactosidase	-	3.2.1.23
[Ref.: #65462]	beta-glucosidase	+	3.2.1.21
[Ref.: #65462]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65462]	cystine arylamidase	+	3.4.11.3
[Ref.: #65462]	esterase (C 4)	+
[Ref.: #65462]	esterase Lipase (C 8)	+
[Ref.: #65462]	gelatinase	+
[Ref.: #65462]	leucine arylamidase	+	3.4.11.1
[Ref.: #65462]	lipase (C 14)	+
[Ref.: #65462]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65462]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #65462]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65462]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65462]	trypsin	-	3.4.21.4
[Ref.: #65462]	urease	-	3.5.1.5
[Ref.: #65462]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65462]	Sample type/isolated from	a surface seawater sample during cruise DY105-17A of the R/V Da-Yang Yi-Hao, at site IR-CTD13
[Ref.: #65462]	Sampling date	December 2005
[Ref.: #65462]	Geographic location (country and/or sea, region)	Indian Ocean
[Ref.: #65462]	Geographic location	-24.2666°/69.7833°
[Ref.: #65462]	Enrichment culture	PTA marine agar medium
[Ref.: #65462]	Enrichment culture composition	per litre of sea water; 2.0 g sodium acetate, 0.5 g tryptone, 0.5 g yeast extract, 0.5 g potato extract, 0.2 g glucose, 0.2 g sucrose, 2.0 g NH 4 NO 3; pH 7.4-7.5
[Ref.: #65462]	Isolation/enrichment procedure	Crude oil was used as a carbon and energy source to enrich oil-degrading bacteria. Strain S27-2 was isolated by using a plate-screening method and purified by restreaking it onto plates of PTA marine agar medium
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Surface water

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65462]	16S rRNA gene sequence	KX977388		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Neptunicella marina S27-2	GCA_014270035	contig	GenBank	2125989 tax ID	*

[Ref.: #65462]

GC-content

43.7 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Neptunicella marina S27-2 NCBI: GCA_014270035.1
[Ref.: #69480]	flagellated	yes	79.37	no
[Ref.: #69480]	gram-positive	no	99.175	no
[Ref.: #69480]	anaerobic	no	98.13	yes
[Ref.: #69480]	aerobic	yes	90.074	yes
[Ref.: #69480]	halophile	no	54.777	no
[Ref.: #69480]	spore-forming	no	96.876	no
[Ref.: #69480]	thermophile	no	99.409	no
[Ref.: #69480]	glucose-util	yes	95.65	yes
[Ref.: #69480]	motile	yes	92.514	yes
[Ref.: #69480]	glucose-ferment	no	73.759	no

Availability in culture collections External links

[Ref.: #65462]

Culture collection no.

KCTC 52335, MCCC 1A02149

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neptunicella marina gen. nov., sp. nov., isolated from surface seawater.	Liu X, Lai Q, Du Y, Zhang X, Liu Z, Sun F, Shao Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.002660	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65462

Xiupian Liu, Qiliang Lai, Yaping Du, Xiaorong Zhang, Zhen Liu, Fengqin Sun, Zongze Shao: Neptunicella marina gen. nov., sp. nov., isolated from surface seawater. IJSEM 68: 1423 - 1428 2018 ( DOI 10.1099/ijsem.0.002660 , PubMed 29533174 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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