Name: Marmoricola silvestris S20-100
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158712

Type strain:

Species: Marmoricola silvestris

Strain Designation: S20-100

Culture col. no.: LMG 30008, DSM 104694

NCBI tax ID(s): 1645666 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Marmoricola silvestris S20-100 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from alpine forest soil at Schwarzseespitze Ritten at 2000 m above sea level.

colony-forming
Gram-positive
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Nocardioidaceae*
	Genus	Marmoricola
	Species	Marmoricola silvestris
	Full Scientific Name (LPSN)	Marmoricola silvestris Schumann et al. 2018

Synonym

"Nocardioides silvestris"

Information on morphological and physiological properties Morphology

[Ref.: #65454]	Gram stain	positive

[Ref.: #65454]	Cell length	0.8-1.6 µm

[Ref.: #65454]	Cell width	0.5-0.7 µm

[Ref.: #65454]	Cell shape	rod-shaped

[Ref.: #65454]	Motility	yes

[Ref.: #65454]	Flagellum arrangement	monopolar

[Ref.: #65454]	Colony color	creamy white
[Ref.: #65454]	Colony shape	circular
[Ref.: #65454]	Cultivation medium used	Reasoner's 2A agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Tryptic Soy Agar

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65454]		growth	0-30 °C
[Ref.: #65454]		optimum	25 °C
[Ref.: #65454]	negative	growth	35 °C

	pH	Kind of pH		pH
[Ref.: #65454]			optimum	7
[Ref.: #65454]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65454]	Oxygen tolerance	aerobe
[Ref.: #65454]	Oxygen tolerance	microaerophile
[Ref.: #65454]	Oxygen tolerance	obligate aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65454]		NaCl		growth	0-1	%(w/v)
[Ref.: #65454]		NaCl		optimum	0	%(w/v)

[Ref.: #65454]	Observation	polar lipid profile contains diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts
[Ref.: #65454]	Observation	contains MK-8(H 4 ) as the major isoprenoid quinone and minor amounts of MK-8 and MK-8(H2)
[Ref.: #65454]	Observation	the cell-wall peptidoglycan contains LL-Dpm as the diagnostic diamino acid and is of the type A3g LL-Dpm - Gly

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65454]	arginine ChEBI	-	hydrolysis
[Ref.: #65454]	benzoate ChEBI	-	hydrolysis
[Ref.: #65454]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #65454]	cellobiose ChEBI	-	hydrolysis
[Ref.: #65454]	D-fructose ChEBI	-	hydrolysis
[Ref.: #65454]	D-mannose ChEBI	-	hydrolysis
[Ref.: #65454]	D-xylose ChEBI	-	hydrolysis
[Ref.: #65454]	esculin ChEBI	+	hydrolysis
[Ref.: #65454]	fumarate ChEBI	+	carbon source
[Ref.: #65454]	galactose ChEBI	-	hydrolysis
[Ref.: #65454]	gelatin ChEBI	+	hydrolysis
[Ref.: #65454]	glucose ChEBI	+	carbon source
[Ref.: #65454]	glucose ChEBI	+	fermentation
[Ref.: #65454]	glycine ChEBI	-	hydrolysis
[Ref.: #65454]	L-arabinose ChEBI	-	hydrolysis
[Ref.: #65454]	lactose ChEBI	-	hydrolysis
[Ref.: #65454]	maltose ChEBI	-	hydrolysis
[Ref.: #65454]	N-acetylglucosamine ChEBI	-	hydrolysis
[Ref.: #65454]	nitrate ChEBI	-	reduction
[Ref.: #65454]	pyruvate ChEBI	+	carbon source
[Ref.: #65454]	starch ChEBI	-	hydrolysis
[Ref.: #65454]	succinate ChEBI	+	carbon source
[Ref.: #65454]	sucrose ChEBI	-	hydrolysis
[Ref.: #65454]	trehalose ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65454]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65454]	acid phosphatase	+	3.1.3.2
[Ref.: #65454]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65454]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65454]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65454]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65454]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65454]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65454]	amylase	-
[Ref.: #65454]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65454]	beta-galactosidase	+	3.2.1.23
[Ref.: #65454]	beta-glucosidase	+	3.2.1.21
[Ref.: #65454]	catalase	+	1.11.1.6
[Ref.: #65454]	cellulase	-	3.2.1.4
[Ref.: #65454]	cystine arylamidase	-	3.4.11.3
[Ref.: #65454]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65454]	esterase Lipase (C 8)	+
[Ref.: #65454]	leucine arylamidase	+	3.4.11.1
[Ref.: #65454]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65454]	pyrazinamidase	+	3.5.1.B15
[Ref.: #65454]	skimmed milk protease	+
[Ref.: #65454]	trypsin	-	3.4.21.4
[Ref.: #65454]	urease	-	3.5.1.5
[Ref.: #65454]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65454]

Fatty acid	Percentage
C16:0 iso	21.7
C18:1ω9c	15.5
C17:1ω6c	11.6
C18:010-methyl	8.8
C17:1ω8c	8.3
C16:1 iso H	6.7
C17:010-methyl	6.6
C16:1ω7c	2.9
C15:0 iso	2.7
C18:0	2.1
C17:0	1.8
C17:0 iso	1.6
C18:0 iso	1.4
C16:0 10-methyl	1.4
C18:1ω7c	1
C16:0	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65454]	Sample type/isolated from	alpine forest soil at Schwarzseespitze Ritten at 2000 m above sea level
[Ref.: #65454]	Sampling date	August 2014
[Ref.: #65454]	Geographic location (country and/or sea, region)	South Tyrol
[Ref.: #65454]	Country	Italy
[Ref.: #65454]	Country ISO 3 Code	ITA
[Ref.: #65454]	Continent	Europe
[Ref.: #65454]	Geographic location	46.5892°/11.4506°
[Ref.: #65454]	Isolation/enrichment procedure	Ten grams of soil sample were shaken with 90 ml sterile 0.1 % sodium pyrophosphate for 15 min at 150 r.p.m. Appropriate dilutions, prepared with sterile saline solution (0.9 % w/v), were plated on Reasoner's 2A (R2A) agar amended with cycloheximide (400 µg/ml) to exclude fungal growth and were incubated at 20C.
* marker position based on {}

Isolation sources categories

#Climate	#Cold	#Alpine
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

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Global distribution of 16S sequence KP899230 (>99% sequence identity) for Marmoricola silvestris from Microbeatlas

Aquatic Samples	40
Soil Samples	90
Animal Samples	28
Plant Samples	25
Total Samples	183

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65454]	Marmoricola silvestris strain S20-100 16S ribosomal RNA gene, partial sequence	KP899230		GenBank ENA

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GC-content

66.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65454]

Culture collection no.

LMG 30008, DSM 104694

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marmoricola silvestris sp. nov., a novel actinobacterium isolated from alpine forest soil.	Schumann P, Zhang DC, Franca L, Margesin R	Int J Syst Evol Microbiol	10.1099/ijsem.0.002674	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65454

Peter Schumann, De-Chao Zhang, Luís França, Rosa Margesin: Marmoricola silvestris sp. nov., a novel actinobacterium isolated from alpine forest soil. IJSEM 68: 1313 - 1318 2018 ( DOI 10.1099/ijsem.0.002674 , PubMed 29498616 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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