Roseisolibacter agri (LMG 29977, DSM 104292, AW1220)

Name: Roseisolibacter agri (LMG 29977, DSM 104292, AW1220)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158703

Type strain

Strain Designation AW1220

Culture col. no. LMG 29977 DSM 104292 AW1220

NCBI tax ID(s) 2014610

Special Collections DSMZ Literature strains

History DSMZ <- A. Geppert; Leibniz Institute DSMZ, Braunschweig, DEUTSCHLAND; AW1220<-A. Geppert<-B. Fösel

Links

Roseisolibacter agri AW1220 is an aerobe, chemoorganotroph, rod-shaped bacterium that forms circular colonies and was isolated from soil sampled at an agricultural floodplain located in Mashare.

rod-shaped colony-forming aerobe chemoorganotroph 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398548

LMG 29977,DSM 104292

@ref 20215

Last LPSN update 2026-05-29 08:44:01

Domain Bacteria

Phylum Gemmatimonadota

Class Gemmatimonadia

Order Gemmatimonadales

Family Gemmatimonadaceae

Genus Roseisolibacter

Species Roseisolibacter agri

Full scientific name Roseisolibacter agri Pascual et al. 2018

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Gram stain
65443	0.6-16 µm	0.4-0.5 µm	rod-shaped
65443				negative
65443

Colony morphology

@ref	Colony color	Colony shape	Medium used
65443	pink	circular	solid medium
65443	white-pinkish		liquid medium

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
65443	solid medium
65443	liquid medium
66489	GEMMATIMONAS MEDIUM (DSMZ Medium 1646)	Medium recipe at MediaDive	Name: GEMMATIMONAS MEDIUM (DSMZ Medium 1646) Composition: Agar 14.0 g/l HEPES 1.95 g/l Peptone 0.5 g/l CaSO4 x 2 H2O 0.43 g/l MgSO4 x 7 H2O 0.37 g/l Yeast extract 0.25 g/l Glucose 0.1 g/l p-Aminobenzoic acid 0.00025 g/l alpha-lipoic acid 0.00025 g/l Thiamine-HCl x 2 H2O 0.00025 g/l Vitamin B12 0.00025 g/l Pantothenic acid 0.00025 g/l Riboflavin 0.00025 g/l Nicotinic acid 0.000125 g/l Nicotine amide 0.000125 g/l Biotin 0.0001 g/l Folic acid 0.0001 g/l Pyridoxamine hydrochloride 5e-05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
65443	positive	growth	15-36
65443	positive	optimum	20-25

Culture pH

@ref	Ability	Type	PH
65443	positive	optimum	7.0-7.5
65443	positive	growth	6.4-8.4

Physiology and metabolism

Oxygen tolerance

65443

Oxygen toleranceaerobe

Nutrition type

65443

Typechemoorganotroph

Spore formation

65443

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65443	NaCl	positive	growth	0-0.25 %(w/v)

Observation

@ref	Observation
65443	cells can elongate up to 35 µm and form filaments
65443	generation time under optimal conditions is 25.3 h
65443	non-capsule-forming

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65443	52682 ChEBI	1,2-butandiol	-	assimilation
65443	16997 ChEBI	1,2-propandiol	-	assimilation
65443	62064 ChEBI	2,3-butanediol	-	assimilation
65443	35686 ChEBI	2-butanol	-	assimilation
65443	64552 ChEBI	2-hydroxybutyrate	-	assimilation
65443		2-oxogluconate	-	assimilation
65443	28644 ChEBI	2-oxopentanoate	-	assimilation
65443	16724 ChEBI	4-hydroxybutyrate	+	growth
65443	39150 ChEBI	4-oxopentanoate	-	assimilation
65443	15688 ChEBI	acetoin	-	assimilation
65443	17128 ChEBI	adipate	-	assimilation
65443	22605 ChEBI	arabinitol	-	assimilation
65443	22599 ChEBI	arabinose	-	assimilation
65443	22651 ChEBI	ascorbate	-	assimilation
65443	16150 ChEBI	benzoate	-	assimilation
65443	8295 ChEBI	beta-hydroxybutyrate	+	growth
65443	17968 ChEBI	butyrate	+	growth
65443		casamino acids	+	growth
65443		casein hydrolysate	+	growth
65443	17057 ChEBI	cellobiose	-	assimilation
65443	35899 ChEBI	crotonate	-	assimilation
65443	18333 ChEBI	D-arabitol	-	assimilation
65443	29990 ChEBI	D-aspartate	-	assimilation
65443	15824 ChEBI	D-fructose	-	assimilation
65443	12936 ChEBI	D-galactose	-	assimilation
65443	18391 ChEBI	D-gluconate	-	assimilation
65443	17634 ChEBI	D-glucose	-	assimilation
65443	62318 ChEBI	D-lyxose	-	assimilation
65443	16899 ChEBI	D-mannitol	-	assimilation
65443	16024 ChEBI	D-mannose	-	assimilation
65443	17924 ChEBI	D-sorbitol	-	assimilation
65443	27689 ChEBI	decanoate	-	assimilation
65443	115156 ChEBI	disodium fumarate	-	assimilation
65443	33946 ChEBI	erythrose	-	assimilation
65443	23958 ChEBI	erythrulose	-	assimilation
65443	16236 ChEBI	ethanol	-	assimilation
65443	30742 ChEBI	ethylene glycol	-	assimilation
65443		fermented rumen extract	+	growth
65443	15740 ChEBI	formate	+	growth
65443	16813 ChEBI	galactitol	-	assimilation
65443	5417 ChEBI	glucosamine	-	assimilation
65443	17234 ChEBI	glucose	-	fermentation
65443	24297 ChEBI	glucuronate	-	assimilation
65443	17754 ChEBI	glycerol	-	assimilation
65443	15428 ChEBI	glycine	+	growth
65443	29805 ChEBI	glycolate	-	assimilation
65443	36655 ChEBI	glyoxylate	-	assimilation
65443	28304 ChEBI	heparin	-	assimilation
65443	27570 ChEBI	histidine	-	assimilation
65443	24741 ChEBI	hydroxyproline	-	assimilation
65443	16411 ChEBI	indole-3-acetic acid	+	growth
65443	48944 ChEBI	isobutyrate	+	growth
65443	16087 ChEBI	isocitrate	-	assimilation
65443	48942 ChEBI	isovalerate	-	assimilation
65443	16977 ChEBI	L-alanine	-	assimilation
65443	16467 ChEBI	L-arginine	-	assimilation
65443	17196 ChEBI	L-asparagine	-	assimilation
65443	16283 ChEBI	L-cystine	-	assimilation
65443	18287 ChEBI	L-fucose	-	assimilation
65443	29985 ChEBI	L-glutamate	+	growth
65443	18050 ChEBI	L-glutamine	-	assimilation
65443	17191 ChEBI	L-isoleucine	-	assimilation
65443	15603 ChEBI	L-leucine	-	assimilation
65443	18019 ChEBI	L-lysine	-	assimilation
65443	17203 ChEBI	L-proline	-	assimilation
65443	62345 ChEBI	L-rhamnose	-	assimilation
65443	17266 ChEBI	L-sorbose	-	assimilation
65443	24996 ChEBI	lactate	-	assimilation
65443	17716 ChEBI	lactose	-	assimilation
65443	6364 ChEBI	laminarin	-	assimilation
65443	25115 ChEBI	malate	-	assimilation
65443	17306 ChEBI	maltose	-	assimilation
65443	6731 ChEBI	melezitose	-	assimilation
65443	17790 ChEBI	methanol	-	assimilation
65443	16811 ChEBI	methionine	-	assimilation
65443	17268 ChEBI	myo-inositol	-	assimilation
65443	506227 ChEBI	N-acetylglucosamine	-	assimilation
65443	32544 ChEBI	nicotinate	-	assimilation
65443	17632 ChEBI	nitrate	-	reduction
65443	25646 ChEBI	octanoate	-	assimilation
65443	18257 ChEBI	ornithine	-	assimilation
65443	30623 ChEBI	oxalate	-	assimilation
65443		peptone	+	growth
65443	28044 ChEBI	phenylalanine	+	growth
65443	28831 ChEBI	propanol	-	assimilation
65443	36241 ChEBI	protocatechuate	+	growth
65443	16634 ChEBI	raffinose	-	assimilation
65443	15963 ChEBI	ribitol	+	growth
65443	17822 ChEBI	serine	+	growth
65443	36208 ChEBI	shikimate	-	assimilation
65443	53258 ChEBI	sodium citrate	-	assimilation
65443	62983 ChEBI	sodium malonate	-	assimilation
65443	132106 ChEBI	sodium propionate	-	assimilation
65443	50144 ChEBI	sodium pyruvate	-	assimilation
65443	63675 ChEBI	sodium succinate	-	assimilation
65443	17992 ChEBI	sucrose	-	assimilation
65443	30929 ChEBI	tartrate	-	assimilation
65443	26986 ChEBI	threonine	-	assimilation
65443	27082 ChEBI	trehalose	-	assimilation
65443		trimethoxybenzoate	-	assimilation
65443	27897 ChEBI	tryptophan	-	assimilation
65443	53426 ChEBI	tween 80	-	assimilation
65443	18186 ChEBI	tyrosine	+	growth
65443	27266 ChEBI	valine	-	assimilation
65443	17151 ChEBI	xylitol	-	assimilation
65443	18222 ChEBI	xylose	-	assimilation
65443		yeast extract (0.01 %, w/v)	+	growth

Metabolite production

@ref	Chebi-ID	Metabolite	Production
65443	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
65443	acid phosphatase	+	3.1.3.2
65443	alkaline phosphatase	+	3.1.3.1
65443	alpha-chymotrypsin	+	3.4.21.1
65443	alpha-fucosidase	-	3.2.1.51
65443	alpha-galactosidase	-	3.2.1.22
65443	alpha-glucosidase	-	3.2.1.20
65443	alpha-mannosidase	-	3.2.1.24
65443	arginine dihydrolase	-	3.5.3.6
65443	beta-galactosidase	-	3.2.1.23
65443	beta-glucosidase	-	3.2.1.21
65443	beta-glucuronidase	-	3.2.1.31
65443	cystine arylamidase	-	3.4.11.3
65443	esterase (C 4)	-
65443	esterase Lipase (C 8)	-
65443	gelatinase	+
65443	leucine arylamidase	+	3.4.11.1
65443	lipase (C 14)	-
65443	N-acetyl-beta-glucosaminidase	+	3.2.1.52
65443	naphthol-AS-BI-phosphohydrolase	-
65443	trypsin	+	3.4.21.4
65443	urease	-	3.5.1.5
65443	valine arylamidase	-

Fatty acid profile

Metadata FA analysis

@ref

65443

Fatty acid	Percentage
C15:0 iso	35.4
C16:1ω7c and/or C15:0 Iso 2OH	28.7
iso-C 17:1ω9c	7.6
C15:0 iso 3OH	5.6
C16:0	5.5
C14:0	4.2
C15:0 anteiso	2.9
C16:0 iso	2.1
C16:1ω5c	1.5
C14:0 3OH and/or C16:1 iso I	1.4
C17:0 iso	1.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	-

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
65443	soil sampled at an agricultural floodplain located in Mashare	2012	Kavango region	Namibia	NAM	Africa	-17.8923	20.1876 -17.8923/20.1876
66489	semi-arid agricultural soil		Mashare (17.89233° S, 20.18761° E)	Namibia	NAM	Africa

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Database
65443	Roseisolibacter agri strain AW1220 16S ribosomal RNA gene, partial sequence	MF363155

GC content

@ref	GC-content (mol%)	Method
65443	73.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseisolibacter agri gen. nov., sp. nov., a novel slow-growing member of the under-represented phylum Gemmatimonadetes.	Pascual J, Foesel BU, Geppert A, Huber KJ, Boedeker C, Luckner M, Wanner G, Overmann J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002619	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65443	Javier Pascual, Bärbel U. Foesel, Alicia Geppert, Katharina J. Huber, Christian Boedeker, Manja Luckner, Gerhard Wanner, Jörg Overmann: Roseisolibacter agri gen. nov., sp. nov., a novel slow-growing member of the under represented phylum Gemmatimonadetes. IJSEM 68: 1028 - 1036 2018 ( DOI 10.1099/ijsem.0.002619 , PubMed 29458671 )
#66489	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104292
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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About us

Roseisolibacter agri (LMG 29977, DSM 104292, AW1220)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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