Name: Polaribacter staleyi 10Alg 139
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158689

Type strain:

Species: Polaribacter staleyi

Strain Designation: 10Alg 139

Culture col. no.: KCTC 52773, KMM 6729

Strain history: <- Nedashkovskaya O.I., G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS

NCBI tax ID(s): 2022337 (species)

Section

Polaribacter staleyi 10Alg 139 is a facultative anaerobe, heterotroph, Gram-negative bacterium that forms circular colonies and was isolated from the red alga Ahnfeltia tobuchiensis.

colony-forming
Gram-negative
motile
rod-shaped
facultative anaerobe
heterotroph
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Polaribacter*
	Species	**Polaribacter staleyi**
	Full Scientific Name (LPSN)	Polaribacter staleyi Nedashkovskaya et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65426]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #65426]	Cell length	1.1-2.5 µm

[Ref.: #65426]	Cell width	0.3-0.4 µm

[Ref.: #65426]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #65426]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #65426]	Flagellum arrangement	gliding
[Ref.: #67771]	Flagellum arrangement	gliding

[Ref.: #65426]	Colony size	2-3 mm
[Ref.: #65426]	Colony color	yellow
[Ref.: #65426]	Colony shape	circular
[Ref.: #65426]	Cultivation medium used	marine agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65426]

Culture medium

Marine agar (MA)

[Ref.: #65426]

Culture medium growth

		Temperature
[Ref.: #65426]	growth	4-34 °C
[Ref.: #65426]	optimum	25-28 °C
[Ref.: #67771]	growth	25-28 °C

[Ref.: #65426]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65426]			optimum	7.0-7.5
[Ref.: #65426]			growth	5.5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65426]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	facultative anaerobe

[Ref.: #65426]

Nutrition type

heterotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65426]		NaCl		growth	0.5-6	%
[Ref.: #65426]		NaCl		optimum	3-4	%

[Ref.: #65426]	Observation	the polar lipid profile consists of phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids
[Ref.: #65426]	Observation	the main respiratory quinone is menaquinone 6 (MK-6)
[Ref.: #67771]	Observation	quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65426]	casein	-	hydrolysis
[Ref.: #65426]	cellobiose ChEBI	+	assimilation
[Ref.: #65426]	cellobiose ChEBI	+	builds acid from
[Ref.: #65426]	chitin ChEBI	-	hydrolysis
[Ref.: #65426]	D-fructose ChEBI	+	assimilation
[Ref.: #65426]	D-fructose ChEBI	+	builds acid from
[Ref.: #65426]	D-galactose ChEBI	+	assimilation
[Ref.: #65426]	D-galactose ChEBI	+	builds acid from
[Ref.: #65426]	D-glucose ChEBI	+	assimilation
[Ref.: #65426]	D-glucose ChEBI	+	builds acid from
[Ref.: #65426]	D-glucose ChEBI	+	fermentation
[Ref.: #65426]	D-xylose ChEBI	+	assimilation
[Ref.: #65426]	D-xylose ChEBI	+	builds acid from
[Ref.: #65426]	dna ChEBI	+	hydrolysis
[Ref.: #65426]	esculin ChEBI	+	hydrolysis
[Ref.: #65426]	gelatin ChEBI	+	hydrolysis
[Ref.: #65426]	glutamyl-glutamic acid ChEBI	+	assimilation
[Ref.: #65426]	glycerol ChEBI	+	builds acid from
[Ref.: #65426]	glycerol 3-phosphate ChEBI	+	assimilation
[Ref.: #65426]	hydroxyethylcellulose ChEBI	-	hydrolysis
[Ref.: #65426]	isocitrate ChEBI	-	builds acid from
[Ref.: #65426]	isocitrate ChEBI	+	assimilation
[Ref.: #65426]	L-alanine ChEBI	+	assimilation
[Ref.: #65426]	L-arabinose ChEBI	-	assimilation
[Ref.: #65426]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65426]	L-asparagine ChEBI	+	assimilation
[Ref.: #65426]	L-leucine ChEBI	+	assimilation
[Ref.: #65426]	L-proline ChEBI	+	assimilation
[Ref.: #65426]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65426]	L-threonine ChEBI	+	assimilation
[Ref.: #65426]	L-tryptophan ChEBI	+	assimilation
[Ref.: #65426]	L-valine ChEBI	+	assimilation
[Ref.: #65426]	lactose ChEBI	+	assimilation
[Ref.: #65426]	lactose ChEBI	+	builds acid from
[Ref.: #65426]	maltose ChEBI	+	assimilation
[Ref.: #65426]	maltose ChEBI	+	builds acid from
[Ref.: #65426]	mannitol ChEBI	+	assimilation
[Ref.: #65426]	mannitol ChEBI	+	builds acid from
[Ref.: #65426]	mannose ChEBI	+	assimilation
[Ref.: #65426]	mannose ChEBI	+	builds acid from
[Ref.: #65426]	melibiose ChEBI	-	assimilation
[Ref.: #65426]	melibiose ChEBI	-	builds acid from
[Ref.: #65426]	methionine ChEBI	+	assimilation
[Ref.: #65426]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65426]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65426]	nitrate ChEBI	-	reduction
[Ref.: #65426]	raffinose ChEBI	-	builds acid from
[Ref.: #65426]	ribose ChEBI	+	assimilation
[Ref.: #65426]	ribose ChEBI	+	builds acid from
[Ref.: #65426]	sodium acetate ChEBI	-	builds acid from
[Ref.: #65426]	starch ChEBI	+	hydrolysis
[Ref.: #65426]	sucrose ChEBI	+	assimilation
[Ref.: #65426]	sucrose ChEBI	+	builds acid from
[Ref.: #65426]	trehalose ChEBI	-	builds acid from
[Ref.: #65426]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65426]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65426]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65426]	tyrosine ChEBI	+	hydrolysis
[Ref.: #65426]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65426]	acetone ChEBI	no
[Ref.: #65426]	hydrogen sulfide ChEBI	no
[Ref.: #65426]	indole ChEBI	no
[Ref.: #65426]	polygalacturonic acid ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #65426]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65426]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65426]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65426]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65426]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65426]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65426]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65426]	beta-glucosidase	+	3.2.1.21
[Ref.: #65426]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65426]	catalase	+	1.11.1.6
[Ref.: #65426]	cystine arylamidase	+	3.4.11.3
[Ref.: #65426]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65426]	endo-1,3(4)-beta-glucanase	+	3.2.1.6
[Ref.: #65426]	esterase (C 4)	+
[Ref.: #65426]	esterase Lipase (C 8)	+
[Ref.: #65426]	leucine arylamidase	+	3.4.11.1
[Ref.: #65426]	lipase (C 14)	-
[Ref.: #65426]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65426]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65426]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65426]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65426]	phosphatase	+
[Ref.: #65426]	trypsin	+	3.4.21.4
[Ref.: #65426]	tryptophan deaminase	-	4.1.99.1
[Ref.: #65426]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65426]

Fatty acid	Percentage
iso-C15:0-3OH	25.9
C15:0 iso	8.7
C13:0 iso	7.6
C15:0	5.9
C15:1ω6c	5.1
C14:0 iso	4.5
C16:0-3OH	3.5
iso-C16:0-3OH	3.3
C16:0	3.1
C15:0 anteiso	2.8
C15:1 iso G	2.6
C16:1ω7c and/or ?16:1ω6c	2.2
C13:0	1.6
C18:0	1.5
iso-C17:0-3OH	1.4
C15:0-3OH	1.4
C14:0	1.3
C16:0 iso	1.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65426]	Sample type/isolated from	the red alga Ahnfeltia tobuchiensis
[Ref.: #65426]	Geographic location (country and/or sea, region)	Troitsa Bay, the Sea of Japan
[Ref.: #65426]	Country	Russia
[Ref.: #65426]	Country ISO 3 Code	RUS
[Ref.: #65426]	Continent	Asia
[Ref.: #65426]	Enrichment culture	marine agar 2216
[Ref.: #65426]	Enrichment culture temperature	28 ̊C

[Ref.: #67771]	Sample type/isolated from	From red alga `Ahnfeltia` sp.
[Ref.: #67771]	Geographic location (country and/or sea, region)	Troitsa Bay, the East Sea(the Sea of Japan)
[Ref.: #67771]	Country	Russia
[Ref.: #67771]	Country ISO 3 Code	RUS
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host

#Algae

#Red algae

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65426]	16S rRNA gene sequence	MF527139		GenBank

[Ref.: #65426]	GC-content	31.8 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67771]	GC-content	31.8 mol%

Availability in culture collections External links

[Ref.: #65426]

Culture collection no.

KCTC 52773, KMM 6729

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polaribacter staleyi sp. nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.	Nedashkovskaya OI, Kim SG, Balabanova LA, Zhukova NV, Bakunina IY, Mikhailov VV	Int J Syst Evol Microbiol	10.1099/ijsem.0.002554	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65426

Olga I. Nedashkovskaya, Song-Gun Kim, Larissa A. Balabanova, Natalia V. Zhukova, Irina Y. Bakunina, Valery V. Mikhailov: Polaribacter staleyi sp. nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis. IJSEM 68: 623 - 629 2018 ( DOI 10.1099/ijsem.0.002554 , PubMed 29303693 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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