Name: Roseovarius salinarum N53
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158669

Type strain:

Species: Roseovarius salinarum

Strain Designation: N53

Culture col. no.: MCCC 1H00200, KCTC 52886

Strain history: <- Zongjun Du, Shandong Univ.

NCBI tax ID(s): 1981892 (species)

Section

Roseovarius salinarum N53 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from sediment sample from a marine solar saltern.

colony-forming
Gram-negative
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Roseovarius*
	Species	**Roseovarius salinarum**
	Full Scientific Name (LPSN)	Roseovarius salinarum Wang et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65398]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #65398]	Cell length	2-5.5 µm

[Ref.: #65398]	Cell width	0.3-0.4 µm

[Ref.: #65398]	Cell shape	rod-shaped

[Ref.: #65398]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %95.188

[Ref.: #65398]	Colony size	0.5-2 mm
[Ref.: #65398]	Colony color	greyish-yellow
[Ref.: #65398]	Colony shape	circular
[Ref.: #65398]	Cultivation medium used	marine agar 2216

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Marine agar (MA)

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Culture medium growth

[Ref.: #65398]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #65398]		growth	20 °C
[Ref.: #65398]		growth	50 °C
[Ref.: #65398]		optimum	33 °C
[Ref.: #65398]	no	growth	15 °C
[Ref.: #65398]	no	growth	55 °C
[Ref.: #67771]		growth	37 °C

[Ref.: #65398]	Temperature range	psychrophilic
[Ref.: #65398]	Temperature range	thermophilic
[Ref.: #65398]	Temperature range	mesophilic
[Ref.: #65398]	Temperature range	psychrophilic
[Ref.: #65398]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	mesophilic

		pH
[Ref.: #65398]	optimum	7-8
[Ref.: #65398]	growth	6.5
[Ref.: #65398]	growth	9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65398]		NaCl		growth	2-15	%(w/v)
[Ref.: #65398]		NaCl		optimum	6	%(w/v)

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Observation

Growth is not observed under anaerobic conditions but microaerobic growth is observed on modified MA with or without 0.1 % (w/v) KNO3 .

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65398]	agar ChEBI	-	hydrolysis
[Ref.: #65398]	alginate ChEBI	-	hydrolysis
[Ref.: #65398]	casein	-	hydrolysis
[Ref.: #65398]	cellulose ChEBI	-	hydrolysis
[Ref.: #65398]	D-malate ChEBI	+	carbon source
[Ref.: #65398]	D-ribose ChEBI	+	builds acid from
[Ref.: #65398]	D-tagatose ChEBI	+	builds acid from
[Ref.: #65398]	esculin ChEBI	+	builds acid from
[Ref.: #65398]	L-glutamate ChEBI	+	carbon source
[Ref.: #65398]	L-sorbose ChEBI	+	builds acid from
[Ref.: #65398]	nitrate ChEBI	-	reduction
[Ref.: #65398]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #65398]	starch ChEBI	-	hydrolysis
[Ref.: #65398]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65398]	tween 40 ChEBI	+	carbon source
[Ref.: #65398]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65398]	tween 60 ChEBI	-	hydrolysis
[Ref.: #65398]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #65398]	acetylspiramycin ChEBI	yes	30	µg (disc)
[Ref.: #65398]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65398]	cefotaxime sodium ChEBI	yes	30	µg (disc)
[Ref.: #65398]	ceftriaxone ChEBI	yes	30	µg (disc)
[Ref.: #65398]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #65398]	clarithromycin ChEBI	yes	15	µg (disc)
[Ref.: #65398]	clindamycin ChEBI	yes	30	µg (disc)
[Ref.: #65398]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #65398]	ofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #65398]	penicillin g ChEBI	yes	10	µg (disc)
[Ref.: #65398]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #65398]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #65398]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65398]	bacteriochlorophyll alpha ChEBI	no
[Ref.: #65398]	hydrogen sulfide ChEBI	no
[Ref.: #65398]	indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65398]	catalase	+	1.11.1.6
[Ref.: #65398]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65398]	esterase (C 4)	+
[Ref.: #65398]	esterase Lipase (C 8)	+
[Ref.: #65398]	leucine arylamidase	+	3.4.11.1
[Ref.: #65398]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65398]	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65398]

Fatty acid	Percentage
C18:1ω7c	54
C16:0	17.9
C18:0	4.7
11-Methyl C18:1ω7c	4.2
C18:1ω9c	3.2
Cyclo C19:0ω8c	3
C16:0 2OH	2.7
C17:0	2.4
C12:0 3-OH	1.9
C12:1 3-OH	1.6
C16:1ω7c and/or C15:0 Iso 2OH	1.3
C17:1ω8c	1.1
C10:0 3-OH	0.6
C15:0	0.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65398]	Sample type/isolated from	sediment sample from a marine solar saltern
[Ref.: #65398]	Sampling date	2016-10
[Ref.: #65398]	Geographic location (country and/or sea, region)	Wendeng
[Ref.: #65398]	Country	China
[Ref.: #65398]	Country ISO 3 Code	CHN
[Ref.: #65398]	Continent	Asia
[Ref.: #65398]	Enrichment culture	marine agar 2216
[Ref.: #65398]	Enrichment culture composition	supplemented with 7 % (w/v) NaCl
[Ref.: #65398]	Enrichment culture duration	7 days
[Ref.: #65398]	Enrichment culture temperature	37 ̊C
[Ref.: #65398]	Isolation/enrichment procedure	serial dilution

[Ref.: #67771]	Sample type/isolated from	From sediment of holothurian pond
[Ref.: #67771]	Geographic location (country and/or sea, region)	Rongcheng
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment
#Condition	#Saline	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65398]	16S rRNA gene sequence	KY973962		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Roseovarius salinarum N53	GCA_002834145	scaffold	GenBank	1981892 tax ID	*
[Ref.: #66792]	Roseovarius salinarum N53	2837136897	draft	JGI IMG/M	1981892 tax ID	*

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GC-content

68.1 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Roseovarius salinarum N53 NCBI: GCA_002834145.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Roseovarius salinarum N53 NCBI: GCA_002834145.1
[Ref.: #69480]	flagellated	no	91.017	yes
[Ref.: #69480]	gram-positive	no	97.568	yes
[Ref.: #69480]	anaerobic	no	94.928	no
[Ref.: #69480]	halophile	yes	77.369	no
[Ref.: #69480]	spore-forming	no	95.776	no
[Ref.: #69480]	glucose-util	no	73.978	no
[Ref.: #69480]	aerobic	yes	84.391	yes
[Ref.: #69480]	thermophile	no	85.645	no
[Ref.: #69480]	motile	no	56.788	no
[Ref.: #69480]	glucose-ferment	no	92.477	no

Availability in culture collections External links

[Ref.: #65398]

Culture collection no.

MCCC 1H00200, KCTC 52886

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseovarius salinarum sp. nov., isolated from a marine solar saltern.	Wang NN, Liu ZY, Jiang LX, Li YX, Du ZJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002778	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65398

Nan-Nan Wang, Zuan-Yan Liu, Lai-Xiang Jiang, Ying-Xiu Li, Zong-Jun Du: Roseovarius salinarum sp. nov., isolated from a marine solar saltern. IJSEM 2018: 1986 - 1991 68 ( DOI 10.1099/ijsem.0.002778 , PubMed 29683420 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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