Name: Halomonas saliphila LCB169
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158650

Type strain:

Species: Halomonas saliphila

Strain Designation: LCB169

Culture col. no.: CGMCC 1.15818, KCTC 52618

Strain history: <- Longzhan GAN, Sichuan Univ.

NCBI tax ID(s): 1848458 (species)

Section

Halomonas saliphila LCB169 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from saline soil sample.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Halomonas*
	Species	**Halomonas saliphila**
	Full Scientific Name (LPSN)	Halomonas saliphila Gan et al. 2018

Synonym

"Billgrantia saliphila"

Information on morphological and physiological properties Morphology

[Ref.: #65376]	Gram stain	negative

[Ref.: #65376]	Cell length	1-1.6 µm

[Ref.: #65376]	Cell width	0.3-0.5 µm

[Ref.: #65376]	Cell shape	rod-shaped

[Ref.: #65376]	Motility	yes

[Ref.: #65376]	Flagellum arrangement	peritrichous

[Ref.: #65376]	Colony size	1-2 mm
[Ref.: #65376]	Colony color	pinkish white
[Ref.: #65376]	Colony shape	circular
[Ref.: #65376]	Incubation period	3 days
[Ref.: #65376]	Cultivation medium used	Luria-Bertani agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65376]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #65376]

Culture medium growth

[Ref.: #65376]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65376]	growth	10-52 °C
[Ref.: #65376]	optimum	30 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #65376]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65376]			optimum	8
[Ref.: #65376]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65376]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65376]		NaCl		growth	0-17	%(w/v)
[Ref.: #65376]		NaCl		optimum	10-15	%(w/v)

[Ref.: #65376]

Observation

Produces exopolysaccharide and accumulates poly-b-hydroxybutyrate granules.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65376]	2-hydroxybutyrate ChEBI	+	oxidation
[Ref.: #65376]	3-hydroxybutyrate ChEBI	+	oxidation
[Ref.: #65376]	alpha-D-glucose ChEBI	+	oxidation
[Ref.: #65376]	arbutin ChEBI	+	builds acid from
[Ref.: #65376]	casein	-	hydrolysis
[Ref.: #65376]	cellobiose ChEBI	+	builds acid from
[Ref.: #65376]	D-alanine ChEBI	+	oxidation
[Ref.: #65376]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65376]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65376]	D-fructose ChEBI	+	builds acid from
[Ref.: #65376]	D-fructose ChEBI	+	oxidation
[Ref.: #65376]	D-fucose ChEBI	-	builds acid from
[Ref.: #65376]	D-galactonic acid lactone ChEBI	-	oxidation
[Ref.: #65376]	D-galactose ChEBI	-	builds acid from
[Ref.: #65376]	D-glucose ChEBI	-	fermentation
[Ref.: #65376]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65376]	D-mannitol ChEBI	+	builds acid from
[Ref.: #65376]	D-mannose ChEBI	+	builds acid from
[Ref.: #65376]	D-ribose ChEBI	+	builds acid from
[Ref.: #65376]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #65376]	D-xylose ChEBI	+	builds acid from
[Ref.: #65376]	DL-carnitine ChEBI	-	oxidation
[Ref.: #65376]	dna ChEBI	-	hydrolysis
[Ref.: #65376]	erythritol ChEBI	-	builds acid from
[Ref.: #65376]	esculin ChEBI	+	hydrolysis
[Ref.: #65376]	formate ChEBI	-	oxidation
[Ref.: #65376]	fumarate ChEBI	-	anaerobic growth
[Ref.: #65376]	galactitol ChEBI	-	builds acid from
[Ref.: #65376]	gelatin ChEBI	+	hydrolysis
[Ref.: #65376]	glucose ChEBI	-	fermentation
[Ref.: #65376]	glucose ChEBI	+	oxidation
[Ref.: #65376]	glucose 1-phosphate ChEBI	+	oxidation
[Ref.: #65376]	glycerol ChEBI	+	builds acid from
[Ref.: #65376]	glycogen ChEBI	-	oxidation
[Ref.: #65376]	inosine ChEBI	+	oxidation
[Ref.: #65376]	itaconate ChEBI	-	oxidation
[Ref.: #65376]	L-alaninamide ChEBI	+	oxidation
[Ref.: #65376]	L-arabinose ChEBI	+	builds acid from
[Ref.: #65376]	L-arabinose ChEBI	+	oxidation
[Ref.: #65376]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65376]	L-aspartate ChEBI	-	oxidation
[Ref.: #65376]	L-fucose ChEBI	-	builds acid from
[Ref.: #65376]	L-proline ChEBI	+	oxidation
[Ref.: #65376]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65376]	L-xylose ChEBI	-	builds acid from
[Ref.: #65376]	lactose ChEBI	-	builds acid from
[Ref.: #65376]	maltose ChEBI	+	builds acid from
[Ref.: #65376]	melezitose ChEBI	-	builds acid from
[Ref.: #65376]	melibiose ChEBI	-	oxidation
[Ref.: #65376]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65376]	myo-inositol ChEBI	-	oxidation
[Ref.: #65376]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65376]	nitrate ChEBI	-	anaerobic growth
[Ref.: #65376]	nitrate ChEBI	-	reduction
[Ref.: #65376]	nitrite ChEBI	-	anaerobic growth
[Ref.: #65376]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65376]	quinate ChEBI	-	oxidation
[Ref.: #65376]	raffinose ChEBI	-	builds acid from
[Ref.: #65376]	ribitol ChEBI	-	builds acid from
[Ref.: #65376]	starch ChEBI	-	hydrolysis
[Ref.: #65376]	succinamate ChEBI	+	oxidation
[Ref.: #65376]	sucrose ChEBI	+	builds acid from
[Ref.: #65376]	thymidine ChEBI	+	oxidation
[Ref.: #65376]	trehalose ChEBI	-	oxidation
[Ref.: #65376]	trehalose ChEBI	+	builds acid from
[Ref.: #65376]	turanose ChEBI	-	builds acid from
[Ref.: #65376]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65376]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65376]	tween 60 ChEBI	-	hydrolysis
[Ref.: #65376]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65376]	uridine ChEBI	+	oxidation
[Ref.: #65376]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65376]	amikacin ChEBI	yes	30	µg (disc)
[Ref.: #65376]	amoxicillin ChEBI	yes	10	µg (disc)
[Ref.: #65376]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65376]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #65376]	cefazolin ChEBI				yes	30	µg (disc)
[Ref.: #65376]	cefoperazone ChEBI	yes	75	µg (disc)
[Ref.: #65376]	cefotaxime ChEBI	yes	30	µg (disc)
[Ref.: #65376]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #65376]	ceftazidime ChEBI	yes	30	µg (disc)
[Ref.: #65376]	ceftriaxone ChEBI	yes	30	µg (disc)
[Ref.: #65376]	cephalexin ChEBI	yes	30	µg (disc)
[Ref.: #65376]	cephradine ChEBI	yes	30	µg (disc)
[Ref.: #65376]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #65376]	doxycycline ChEBI	yes	30	µg (disc)
[Ref.: #65376]	erythromycin ChEBI				yes	15	µg (disc)
[Ref.: #65376]	gentamicin ChEBI				yes	10	µg (disc)
[Ref.: #65376]	kanamycin ChEBI				yes	30	µg (disc)
[Ref.: #65376]	minocycline ChEBI	yes	30	µg (disc)
[Ref.: #65376]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #65376]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #65376]	nitrofurantoin ChEBI	yes	300	µg (disc)
[Ref.: #65376]	oxacillin ChEBI				yes	1	µg (disc)
[Ref.: #65376]	penicillin ChEBI				yes	10	Unit (disc)
[Ref.: #65376]	piperacillin ChEBI				yes	100	µg (disc)
[Ref.: #65376]	polymyxin b ChEBI	yes	30	µg (disc)
[Ref.: #65376]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #65376]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #65376]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #65376]	tobramycin ChEBI	yes	10	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65376]	acetoin ChEBI	no
[Ref.: #65376]	hydrogen sulfide ChEBI	yes
[Ref.: #65376]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #65376]		acetoin ChEBI	-
[Ref.: #65376]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #65376]	acid phosphatase	-	3.1.3.2
[Ref.: #65376]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65376]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65376]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65376]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65376]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65376]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65376]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #65376]	beta-galactosidase	-	3.2.1.23
[Ref.: #65376]	beta-glucosidase	-	3.2.1.21
[Ref.: #65376]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65376]	catalase	+	1.11.1.6
[Ref.: #65376]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65376]	esterase (C 4)	+
[Ref.: #65376]	esterase Lipase (C 8)	+
[Ref.: #65376]	leucine arylamidase	+	3.4.11.1
[Ref.: #65376]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65376]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65376]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65376]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #65376]	trypsin	-	3.4.21.4
[Ref.: #65376]	urease	-	3.5.1.5
[Ref.: #65376]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65376]

Fatty acid	Percentage
C18:1ω7c	31.1
C16:0	26.4
C16:1ω7c	10.8
C12:0 3OH	10.1
C19:0 cyclo ω8c	8.1
C18:0	3.8
C12:0	3.1
C17:0 cyclo ω8c	2.9
C10:0	2.5
C12:0 cyclo ω3c	0.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65376]	Sample type/isolated from	saline soil sample
[Ref.: #65376]	Geographic location (country and/or sea, region)	Kazak Autonomous County of Aksay, Gansu Province
[Ref.: #65376]	Country	China
[Ref.: #65376]	Country ISO 3 Code	CHN
[Ref.: #65376]	Continent	Asia
[Ref.: #65376]	Geographic location	39.4073°/94.0775°
[Ref.: #65376]	Enrichment culture	modified saline agar medium
[Ref.: #65376]	Enrichment culture composition	containing per litre, 7.5 g casein peptone, 100 g sodium molybdate, 10 g yeast extract, 1.5 ml MS mixture (0.05 g each of betaine, proline, glycine, D-sorbitol and glutamate, added to 1000 ml H2O), 20 g agar (pH adjusted to 8.0 with HCl/NaOH)
[Ref.: #65376]	Enrichment culture duration	3 days
[Ref.: #65376]	Enrichment culture temperature	37 ̊C
[Ref.: #65376]	Isolation/enrichment procedure	To isolate halophilic bacteria, a saline soil sample (salinity 1.5-2.0 % and pH 8.0-8.2), suspended in 0.9 % physiological saline solution, was spread by the standard dilution-plating technique on a modified saline agar medium for 72 h at 37°C.

[Ref.: #67771]	Sample type/isolated from	From saline-alkali soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Gansu province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65376]	16S rRNA gene sequence	KX008964		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halomonas saliphila LCB169	GCA_002930105	contig	GenBank	1848458 tax ID	*
[Ref.: #66792]	Halomonas saliphila LCB169	2844988376	draft	JGI IMG/M	1848458 tax ID	*

[Ref.: #65376]

GC-content

66.1 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65376]

Culture collection no.

CGMCC 1.15818, KCTC 52618

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halomonas bachuensis sp. nov., Isolated from Gobi Soil.	Xiao Z, Shen J, Wang Z, Dong F, Zhao JY	Curr Microbiol	10.1007/s00284-020-02268-w	2020	*
Phylogeny	Halomonas lactosivorans sp. nov., isolated from salt-lake sediment.	Ming H, Ji WL, Li M, Zhao ZL, Cheng LJ, Niu MM, Zhang LY, Wang Y, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.004209	2020	*
Phylogeny	Halomonas saliphila sp. nov., a moderately halophilic bacterium isolated from a saline soil.	Gan L, Long X, Zhang H, Hou Y, Tian J, Zhang Y, Tian Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.002644	2018	*
Phylogeny	Halomonas antri sp. nov., a carotenoid-producing bacterium isolated from surface seawater.	So Y, Chhetri G, Kim I, Kang M, Kim J, Lee B, Jang W, Seo T	Int J Syst Evol Microbiol	10.1099/ijsem.0.005272	2022	*
Enzymology	Preparation, characterization and functional properties of a novel exopolysaccharide produced by the halophilic strain Halomonas saliphila LCB169(T).	Gan L, Li X, Zhang H, Zhang R, Wang H, Xu Z, Peng B, Tian Y	Int J Biol Macromol	10.1016/j.ijbiomac.2020.04.062	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65376

Longzhan Gan, Xiufeng Long, Heming Zhang, Yanyan Hou, Jiewei Tian, Yuqin Zhang, Yongqiang Tian: Halomonas saliphila sp. nov., a moderately halophilic bacterium isolated from a saline soil. IJSEM 68: 1153 - 1159 2018 ( DOI 10.1099/ijsem.0.002644 , PubMed 29458658 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Halomonas saliphila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF