Name: Bacillus manusensis Ma50-6
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158630

Type strain:

Species: Bacillus manusensis

Strain Designation: Ma50-6

Culture col. no.: KCTC 33882, CCTCC AB 2017019

Strain history: <- Sun Qinglei, Institute of Oceanology, China

NCBI tax ID(s): 2078954 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Bacillus manusensis Ma50-6 is an aerobe, spore-forming, thermophilic bacterium that was isolated from hydrothermal sediments .

Gram-positive
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus manusensis**
	Full Scientific Name (LPSN)	Bacillus manusensis Sun et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65353]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #65353]	Cell length	2-5 µm

[Ref.: #65353]	Cell width	0.4-0.6 µm

[Ref.: #65353]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #65353]	growth	45-70 °C
[Ref.: #65353]	optimum	50-55 °C
[Ref.: #67771]	growth	50 °C

	pH	Kind of pH		pH
[Ref.: #65353]			optimum	7
[Ref.: #65353]			growth	7-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #65353]	Spore description	Ellipsoidal endospores are formed centrally and usually cause the sporangia to swell
[Ref.: #65353]	Type of spore	endospore
[Ref.: #65353]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65353]		NaCl		growth	0-8	%(w/v)
[Ref.: #65353]		NaCl		optimum	3	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65353]	amygdalin ChEBI	-	builds acid from
[Ref.: #65353]	arbutin ChEBI	-	builds acid from
[Ref.: #65353]	casein	-	hydrolysis
[Ref.: #65353]	cellobiose ChEBI	-	builds acid from
[Ref.: #65353]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65353]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65353]	D-fructose ChEBI	-	builds acid from
[Ref.: #65353]	D-fucose ChEBI	-	builds acid from
[Ref.: #65353]	D-galactose ChEBI	-	builds acid from
[Ref.: #65353]	D-glucose ChEBI	-	builds acid from
[Ref.: #65353]	D-glucose ChEBI	+	assimilation
[Ref.: #65353]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65353]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65353]	D-mannitol ChEBI	+	assimilation
[Ref.: #65353]	D-mannose ChEBI	-	assimilation
[Ref.: #65353]	D-mannose ChEBI	-	builds acid from
[Ref.: #65353]	D-ribose ChEBI	-	builds acid from
[Ref.: #65353]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65353]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65353]	D-xylose ChEBI	-	builds acid from
[Ref.: #65353]	decanoate ChEBI	-	assimilation
[Ref.: #65353]	erythritol ChEBI	-	builds acid from
[Ref.: #65353]	esculin ChEBI	-	builds acid from
[Ref.: #65353]	esculin ChEBI	+	hydrolysis
[Ref.: #65353]	esculin ferric citrate	+	builds acid from
[Ref.: #65353]	galactitol ChEBI	-	builds acid from
[Ref.: #65353]	galactose ChEBI	-	builds acid from
[Ref.: #65353]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65353]	glucose ChEBI	+	builds acid from
[Ref.: #65353]	glycerol ChEBI	-	builds acid from
[Ref.: #65353]	glycogen ChEBI	-	builds acid from
[Ref.: #65353]	glycogen ChEBI	+	builds acid from
[Ref.: #65353]	inulin ChEBI	-	builds acid from
[Ref.: #65353]	L-arabinose ChEBI	-	assimilation
[Ref.: #65353]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65353]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65353]	L-fucose ChEBI	-	builds acid from
[Ref.: #65353]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65353]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65353]	L-xylose ChEBI	-	builds acid from
[Ref.: #65353]	lactose ChEBI	-	builds acid from
[Ref.: #65353]	lyxose ChEBI	-	builds acid from
[Ref.: #65353]	maltose ChEBI	+	assimilation
[Ref.: #65353]	maltose ChEBI	-	builds acid from
[Ref.: #65353]	maltose ChEBI	+	builds acid from
[Ref.: #65353]	mannitol ChEBI	+	builds acid from
[Ref.: #65353]	mannose ChEBI	-	builds acid from
[Ref.: #65353]	melezitose ChEBI	-	builds acid from
[Ref.: #65353]	melibiose ChEBI	-	builds acid from
[Ref.: #65353]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65353]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65353]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65353]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65353]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65353]	nitrate ChEBI	-	reduction
[Ref.: #65353]	phenyl beta-D-glucuronide ChEBI	-	hydrolysis
[Ref.: #65353]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65353]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #65353]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65353]	potassium gluconate ChEBI	+	assimilation
[Ref.: #65353]	raffinose ChEBI	-	builds acid from
[Ref.: #65353]	ribitol ChEBI	-	builds acid from
[Ref.: #65353]	salicin ChEBI	-	builds acid from
[Ref.: #65353]	sorbitol ChEBI	-	builds acid from
[Ref.: #65353]	starch ChEBI	-	builds acid from
[Ref.: #65353]	starch ChEBI	-	hydrolysis
[Ref.: #65353]	sucrose ChEBI	-	builds acid from
[Ref.: #65353]	sucrose ChEBI	+	builds acid from
[Ref.: #65353]	trehalose ChEBI	-	builds acid from
[Ref.: #65353]	trehalose ChEBI	+	builds acid from
[Ref.: #65353]	tryptophan ChEBI	-	energy source
[Ref.: #65353]	turanose ChEBI	-	builds acid from
[Ref.: #65353]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65353]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65353]	tween 60 ChEBI	-	hydrolysis
[Ref.: #65353]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65353]	xylitol ChEBI	-	builds acid from
[Ref.: #65353]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65353]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test
[Ref.: #65353]		indole ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #65353]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65353]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65353]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65353]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65353]	beta-galactosidase	-	3.2.1.23
[Ref.: #65353]	catalase	+	1.11.1.6
[Ref.: #65353]	chymotrypsin	+	3.4.4.5
[Ref.: #65353]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65353]	esterase (C 4)	+
[Ref.: #65353]	esterase Lipase (C 8)	+
[Ref.: #65353]	leucine arylamidase	+	3.4.11.1
[Ref.: #65353]	lipase (C 14)	-
[Ref.: #65353]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65353]	tryptophan deaminase	-	4.1.99.1
[Ref.: #65353]	urease	-	3.5.1.5
[Ref.: #65353]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65353]	Sample type/isolated from	hydrothermal sediments (depth of 1851m)
[Ref.: #65353]	Sampling date	2015-06
[Ref.: #65353]	Geographic location (country and/or sea, region)	Manus Basin
[Ref.: #65353]	Country	Pacific Ocean
[Ref.: #65353]	Geographic location	3.7300°/151.6800°
[Ref.: #65353]	Enrichment culture	marine agar
[Ref.: #65353]	Enrichment culture composition	(l-1): peptone, 5.0 g; yeast extract, 1.0 g; Fe(III) citrate, 0.1 g; NaCl, 19.45 g; MgCl2, 5.98 g; Na2SO4, 3.24 g; CaCl2, 1.8 g; KCl, 0.55 g; Na2CO3, 0.16 g; KBr, 0.08 g; SrCl2, 0.034 g; H3BO3, 0.022 g; Na2SiO3, 0.004 g; NaF, 0.0024 g; (NH4)NO3, 0.0016 g; Na2HPO4, 0.008 g; agar 15.0 g.
[Ref.: #65353]	Enrichment culture duration	3 days
[Ref.: #65353]	Enrichment culture temperature	50 ̊C
[Ref.: #65353]	Isolation/enrichment procedure	1.0 g of the sediment sample was suspended with 3 mm sterile seawater, and a 100 µl suspension was spread on marine agar plates. Incubation for 3 days at 50°C under aerobic conditions.

[Ref.: #67771]	Sample type/isolated from	From hydrothermal marine sediments, depth 1851m
[Ref.: #67771]	Geographic location (country and/or sea, region)	Manus Basin, western Pacific Ocean
[Ref.: #67771]	Country	Pacific Ocean
[Ref.: #67771]	Geographic location	-3.7342°/151.6770°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MF582328 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	21
Soil Samples	49
Animal Samples	4
Plant Samples	2
Total Samples	76

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65353]	Bacillus manusensis strain Ma50-6 16S ribosomal RNA gene, partial sequence	MF582328		GenBank ENA

[Ref.: #65353]	GC-content	47.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	47.6 mol%

Availability in culture collections External links

[Ref.: #65353]

Culture collection no.

KCTC 33882, CCTCC AB 2017019

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Bacillus kexueae sp. nov. and Bacillus manusensis sp. nov., isolated from hydrothermal sediments.	Sun QL, Yu C, Luan ZD, Lian C, Hu YH, Sun L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002594	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65353

Qing-lei Sun, Chao Yu, Zhen-dong Luan, Chao Lian, Yong-hua Hu, Li Sun: Description of Bacillus kexueae sp. nov. and Bacillus manusensis sp. nov., isolated from hydrothermal sediments. IJSEM 68: 829 - 834 2018 ( DOI 10.1099/ijsem.0.002594 , PubMed 29458542 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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