Name: Bacillus kexueae Ma50-5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158629

Type strain:

Species: Bacillus kexueae

Strain Designation: Ma50-5

Culture col. no.: KCTC 33881, CCTCC AB 2017020

Strain history: <- Sun Qinglei, Institute of Oceanology, China

NCBI tax ID(s): 2078952 (species)

Section

Bacillus kexueae Ma50-5 is an aerobe, spore-forming, thermophilic bacterium that was isolated from hydrothermal sediments .

Gram-positive
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus kexueae**
	Full Scientific Name (LPSN)	Bacillus kexueae Sun et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65353]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #65353]	Cell length	2-5 µm

[Ref.: #65353]	Cell width	0.4-0.6 µm

[Ref.: #65353]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #65353]	growth	40-60 °C
[Ref.: #65353]	optimum	45 °C
[Ref.: #67771]	growth	50 °C

[Ref.: #65353]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #65353]			optimum	7
[Ref.: #65353]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #65353]	Spore description	Ellipsoidal/cylindrical endospores are formed centrally.
[Ref.: #65353]	Type of spore	endospore
[Ref.: #65353]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65353]		NaCl		growth	0-13	%(w/v)
[Ref.: #65353]		NaCl		optimum	3	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65353]	2-oxogluconate	+	builds acid from
[Ref.: #65353]	amygdalin ChEBI	+	builds acid from
[Ref.: #65353]	casein	+	hydrolysis
[Ref.: #65353]	cellobiose ChEBI	+	builds acid from
[Ref.: #65353]	D-glucose ChEBI	+	carbon source
[Ref.: #65353]	D-glucose ChEBI	+	energy source
[Ref.: #65353]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65353]	D-mannitol ChEBI	+	carbon source
[Ref.: #65353]	D-mannitol ChEBI	+	energy source
[Ref.: #65353]	D-mannose ChEBI	-	carbon source
[Ref.: #65353]	D-mannose ChEBI	-	energy source
[Ref.: #65353]	D-xylose ChEBI	-	builds acid from
[Ref.: #65353]	decanoate ChEBI	-	carbon source
[Ref.: #65353]	decanoate ChEBI	-	energy source
[Ref.: #65353]	esculin ChEBI	+	builds acid from
[Ref.: #65353]	esculin ChEBI	+	hydrolysis
[Ref.: #65353]	galactitol ChEBI	-	builds acid from
[Ref.: #65353]	galactose ChEBI	+	builds acid from
[Ref.: #65353]	glucose ChEBI	+	builds acid from
[Ref.: #65353]	glycogen ChEBI	+	builds acid from
[Ref.: #65353]	inulin ChEBI	-	builds acid from
[Ref.: #65353]	L-xylose ChEBI	-	builds acid from
[Ref.: #65353]	maltose ChEBI	+	carbon source
[Ref.: #65353]	maltose ChEBI	+	energy source
[Ref.: #65353]	maltose ChEBI	+	builds acid from
[Ref.: #65353]	mannitol ChEBI	+	builds acid from
[Ref.: #65353]	mannose ChEBI	-	builds acid from
[Ref.: #65353]	melezitose ChEBI	+	builds acid from
[Ref.: #65353]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65353]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65353]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #65353]	N-acetylglucosamine ChEBI	-	energy source
[Ref.: #65353]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65353]	nitrate ChEBI	-	reduction
[Ref.: #65353]	phenyl beta-D-glucuronide ChEBI	+	hydrolysis
[Ref.: #65353]	phenylacetate ChEBI	-	carbon source
[Ref.: #65353]	phenylacetate ChEBI	-	energy source
[Ref.: #65353]	potassium gluconate ChEBI	+	carbon source
[Ref.: #65353]	potassium gluconate ChEBI	+	energy source
[Ref.: #65353]	raffinose ChEBI	-	builds acid from
[Ref.: #65353]	rhamnose ChEBI	-	builds acid from
[Ref.: #65353]	ribitol ChEBI	-	builds acid from
[Ref.: #65353]	ribose ChEBI	+	builds acid from
[Ref.: #65353]	sodium citrate ChEBI	+	carbon source
[Ref.: #65353]	sodium citrate ChEBI	+	energy source
[Ref.: #65353]	sorbitol ChEBI	-	builds acid from
[Ref.: #65353]	sorbose ChEBI	-	builds acid from
[Ref.: #65353]	starch ChEBI	-	hydrolysis
[Ref.: #65353]	sucrose ChEBI	+	builds acid from
[Ref.: #65353]	trehalose ChEBI	+	builds acid from
[Ref.: #65353]	tryptophan ChEBI	-	energy source
[Ref.: #65353]	turanose ChEBI	-	builds acid from
[Ref.: #65353]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65353]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65353]	tween 60 ChEBI	-	hydrolysis
[Ref.: #65353]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65353]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65353]	amikacin ChEBI	yes	30	µg (disc)
[Ref.: #65353]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65353]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #65353]	cefazolin ChEBI	yes	30	µg (disc)
[Ref.: #65353]	cefoperazone ChEBI	yes	75	µg (disc)
[Ref.: #65353]	ceftazidime ChEBI				yes	30	µg (disc)
[Ref.: #65353]	ceftriaxone ChEBI	yes	30	µg (disc)
[Ref.: #65353]	cefuroxime ChEBI	yes	30	µg (disc)
[Ref.: #65353]	cephalexin ChEBI	yes	30	µg (disc)
[Ref.: #65353]	cephradine ChEBI	yes	30	µg (disc)
[Ref.: #65353]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #65353]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #65353]	clindamycin ChEBI	yes	2	µg (disc)
[Ref.: #65353]	doxycycline ChEBI	yes	30	µg (disc)
[Ref.: #65353]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #65353]	furazolidone ChEBI	yes	300	µg (disc)
[Ref.: #65353]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #65353]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #65353]	midecamycin ChEBI	yes	30	µg (disc)
[Ref.: #65353]	minocycline ChEBI	yes	30	µg (disc)
[Ref.: #65353]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #65353]	norfloxacin ChEBI	yes	10	µg (disc)
[Ref.: #65353]	ofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #65353]	oxacillin ChEBI	yes	1	µg (disc)
[Ref.: #65353]	penicillin ChEBI	yes	10	µg (disc)
[Ref.: #65353]	piperacillin ChEBI	yes	100	µg (disc)
[Ref.: #65353]	polymyxin b ChEBI				yes	300	Unit (disc)
[Ref.: #65353]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #65353]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65353]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test
[Ref.: #65353]		indole ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #65353]	acid phosphatase	+	3.1.3.2
[Ref.: #65353]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65353]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65353]	alpha-galactosidase	+	3.2.1.22
[Ref.: #65353]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65353]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65353]	beta-galactosidase	+	3.2.1.23
[Ref.: #65353]	beta-glucosidase	+	3.2.1.21
[Ref.: #65353]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65353]	catalase	+	1.11.1.6
[Ref.: #65353]	chymotrypsin	+	3.4.4.5
[Ref.: #65353]	cystine arylamidase	+	3.4.11.3
[Ref.: #65353]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65353]	esterase (C 4)	+
[Ref.: #65353]	esterase Lipase (C 8)	+
[Ref.: #65353]	gelatinase	+
[Ref.: #65353]	leucine arylamidase	+	3.4.11.1
[Ref.: #65353]	lipase (C 14)	-
[Ref.: #65353]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65353]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65353]	trypsin	-	3.4.21.4
[Ref.: #65353]	tryptophan deaminase	-	4.1.99.1
[Ref.: #65353]	urease	-	3.5.1.5
[Ref.: #65353]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65353]	Sample type/isolated from	hydrothermal sediments (depth of 1851m)
[Ref.: #65353]	Sampling date	2015-06
[Ref.: #65353]	Geographic location (country and/or sea, region)	Manus Basin
[Ref.: #65353]	Country	Pacific Ocean
[Ref.: #65353]	Geographic location	3.7300°/151.6800°
[Ref.: #65353]	Enrichment culture	marine agar
[Ref.: #65353]	Enrichment culture composition	(l-1): peptone, 5.0 g; yeast extract, 1.0 g; Fe(III) citrate, 0.1 g; NaCl, 19.45 g; MgCl2, 5.98 g; Na2SO4, 3.24 g; CaCl2, 1.8 g; KCl, 0.55 g; Na2CO3, 0.16 g; KBr, 0.08 g; SrCl2, 0.034 g; H3BO3, 0.022 g; Na2SiO3, 0.004 g; NaF, 0.0024 g; (NH4)NO3, 0.0016 g; Na2HPO4, 0.008 g; agar 15.0 g.
[Ref.: #65353]	Enrichment culture duration	3 days
[Ref.: #65353]	Enrichment culture temperature	50 ̊C
[Ref.: #65353]	Isolation/enrichment procedure	1.0 g of the sediment sample was suspended with 3 mm sterile seawater, and a 100 µl suspension was spread on marine agar plates. Incubation for 3 days at 50°C under aerobic conditions.

[Ref.: #67771]	Sample type/isolated from	From hydrothermal marine sediments, depth 1851m
[Ref.: #67771]	Geographic location (country and/or sea, region)	Manus Basin, western Pacific Ocean
[Ref.: #67771]	Country	Pacific Ocean
[Ref.: #67771]	Geographic location	-3.7342°/151.6770°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MF582327 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	58
Soil Samples	24
Animal Samples	9
Plant Samples	24
Total Samples	115

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65353]	16S rRNA gene sequence	MF582327		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus kexueae KCTC 33881	GCA_022809095	contig	GenBank	2078952 tax ID	*

[Ref.: #65353]	GC-content	43.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	43.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus kexueae KCTC 33881 NCBI: GCA_022809095.1
[Ref.: #69480]	flagellated	yes	91.136	no
[Ref.: #69480]	gram-positive	yes	89.437	yes
[Ref.: #69480]	anaerobic	no	98.243	yes
[Ref.: #69480]	aerobic	yes	88.16	no
[Ref.: #69480]	halophile	no	55.016	no
[Ref.: #69480]	spore-forming	yes	97.833	no
[Ref.: #69480]	motile	yes	89.35	no
[Ref.: #69480]	glucose-ferment	no	93.686	no
[Ref.: #69480]	thermophile	no	51.549	no
[Ref.: #69480]	glucose-util	yes	84.13	no

Availability in culture collections External links

[Ref.: #65353]

Culture collection no.

KCTC 33881, CCTCC AB 2017020

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Bacillus kexueae sp. nov. and Bacillus manusensis sp. nov., isolated from hydrothermal sediments.	Sun QL, Yu C, Luan ZD, Lian C, Hu YH, Sun L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002594	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65353

Qing-lei Sun, Chao Yu, Zhen-dong Luan, Chao Lian, Yong-hua Hu, Li Sun: Description of Bacillus kexueae sp. nov. and Bacillus manusensis sp. nov., isolated from hydrothermal sediments. IJSEM 68: 829 - 834 2018 ( DOI 10.1099/ijsem.0.002594 , PubMed 29458542 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Bacillus kexueae KCTC 33881

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