Name: Tsukamurella ocularis HKU63
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158627

Type strain:

Species: Tsukamurella ocularis

Strain Designation: HKU63

Culture col. no.: JCM 31969, DSM 105034

Strain history: J. L. L. Teng; Dept. of Microbiol., Univ. of Hong Kong, China; HKU63.

NCBI tax ID(s): 1970234 (species)

Other strains from Tsukamurella ocularis

T. ocularis JCM 31970, DSM 105035

Section

Tsukamurella ocularis HKU63 is an aerobe, mesophilic, Gram-positive bacterium that forms irregular colonies and was isolated from eye swab of a patient with conjunctivitis.

colony-forming
Gram-positive
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Tsukamurellaceae*
	Genus	*Tsukamurella*
	Species	**Tsukamurella ocularis**
	Full Scientific Name (PNU)	Tsukamurella ocularis Teng et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65352]	Gram stain	positive
[Ref.: #65352]	Motility	no

[Ref.: #65352]	Colony size	2 mm
[Ref.: #65352]	Colony color	white and creamy or yellow and creamy
[Ref.: #65352]	Colony shape	irregular
[Ref.: #65352]	Incubation period	2 days
[Ref.: #65352]	Cultivation medium used	Columbia agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65352]

Culture medium

Columbia agar

[Ref.: #65352]

Culture medium growth

[Ref.: #65352]

Culture medium composition

expand / minimize

[Ref.: #65352]

Culture medium

brain-heart infusion agar

[Ref.: #65352]

Culture medium growth

[Ref.: #65352]

Culture medium

Nutrient agar (NA)

[Ref.: #65352]

Culture medium growth

[Ref.: #65352]

Culture medium

tryptic soy agar

[Ref.: #65352]

Culture medium growth

[Ref.: #65352]

Culture medium

chocolate agar

[Ref.: #65352]

Culture medium growth

[Ref.: #65352]

Culture medium

MacConkey agar

[Ref.: #65352]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65352]		growth	37 °C
[Ref.: #65352]	no	growth	10 °C
[Ref.: #65352]	no	growth	42 °C
[Ref.: #67770]		growth	37 °C

[Ref.: #65352]	Temperature range	mesophilic
[Ref.: #65352]	Temperature range	psychrophilic
[Ref.: #65352]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65352]

Oxygen tolerance

aerobe

[Ref.: #65352]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65352]		NaCl		growth	0-7	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-9

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65352]	amygdalin ChEBI	-	builds acid from
[Ref.: #65352]	arbutin ChEBI	+	builds acid from
[Ref.: #65352]	cellobiose ChEBI	-	builds acid from
[Ref.: #65352]	D-arabinose ChEBI	+	builds acid from
[Ref.: #65352]	D-arabitol ChEBI	+	builds acid from
[Ref.: #65352]	D-fructose ChEBI	+	builds acid from
[Ref.: #65352]	D-fucose ChEBI	-	builds acid from
[Ref.: #65352]	D-galactose ChEBI	+	builds acid from
[Ref.: #65352]	D-glucose ChEBI	+	builds acid from
[Ref.: #65352]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65352]	D-mannitol ChEBI	+	builds acid from
[Ref.: #65352]	D-mannose ChEBI	-	builds acid from
[Ref.: #65352]	D-ribose ChEBI	+	builds acid from
[Ref.: #65352]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #65352]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65352]	D-xylose ChEBI	-	builds acid from
[Ref.: #65352]	erythritol ChEBI	-	builds acid from
[Ref.: #65352]	esculin ferric citrate	+	builds acid from
[Ref.: #65352]	galactitol ChEBI	-	builds acid from
[Ref.: #65352]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65352]	glycerol ChEBI	+	builds acid from
[Ref.: #65352]	glycogen ChEBI	-	builds acid from
[Ref.: #65352]	inulin ChEBI	-	builds acid from
[Ref.: #65352]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65352]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65352]	L-fucose ChEBI	+	builds acid from
[Ref.: #65352]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65352]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65352]	L-xylose ChEBI	-	builds acid from
[Ref.: #65352]	lactose ChEBI	-	builds acid from
[Ref.: #65352]	maltose ChEBI	+	builds acid from
[Ref.: #65352]	melezitose ChEBI	-	builds acid from
[Ref.: #65352]	melibiose ChEBI	-	builds acid from
[Ref.: #65352]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65352]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65352]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65352]	myo-inositol ChEBI	+	builds acid from
[Ref.: #65352]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65352]	potassium 2-dehydro-D-gluconate	+	builds acid from
[Ref.: #65352]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65352]	potassium gluconate ChEBI	+	builds acid from
[Ref.: #65352]	raffinose ChEBI	-	builds acid from
[Ref.: #65352]	ribitol ChEBI	-	builds acid from
[Ref.: #65352]	salicin ChEBI	-	builds acid from
[Ref.: #65352]	starch ChEBI	-	builds acid from
[Ref.: #65352]	sucrose ChEBI	+	builds acid from
[Ref.: #65352]	trehalose ChEBI	+	builds acid from
[Ref.: #65352]	turanose ChEBI	+	builds acid from
[Ref.: #65352]	tyrosine ChEBI	+	hydrolysis
[Ref.: #65352]	xanthine ChEBI	-	hydrolysis
[Ref.: #65352]	xylitol ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65352]		catalase	+	1.11.1.6
[Ref.: #65352]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65352]	Sample type/isolated from	eye swab of a patient with conjunctivitis
[Ref.: #65352]	Country	Hong Kong
[Ref.: #65352]	Country ISO 3 Code	HKG
[Ref.: #65352]	Continent	Asia
[Ref.: #65352]	Enrichment culture	Columbia agar
[Ref.: #65352]	Enrichment culture composition	with 5 % defibrinated sheep blood
[Ref.: #65352]	Enrichment culture temperature	37 ̊C

[Ref.: #67770]	Sample type/isolated from	Eye swab of a patient with conjunctivitis in Hong Kong
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Organ	#Eye
#Infection	#Patient	#Swab
#Infection	#Inflammation	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY787187 (>99% sequence identity) for Tsukamurella from Microbeatlas

Aquatic Samples	2978
Soil Samples	3431
Animal Samples	5160
Plant Samples	790
Total Samples	12359

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65352]	16S rRNA gene sequence	KY787187		GenBank

[Ref.: #67770]

GC-content

69.4-73.2 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65352]

Culture collection no.

JCM 31969, DSM 105034

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tsukamurella ocularis sp. nov. and Tsukamurella hominis sp. nov., isolated from patients with conjunctivitis in Hong Kong.	Teng JLL, Tang Y, Wong SSY, Chiu TH, Zhao Z, Chan E, Ngan AHY, Lau SKP, Woo PCY	Int J Syst Evol Microbiol	10.1099/ijsem.0.002589	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65352

Jade L. L. Teng, Ying Tang, Samson S. Y. Wong, Tsz Ho Chiu, Zhe Zhao, Elaine Chan, Antonio H. Y. Ngan, Susanna K. P. Lau, Patrick C. Y. Woo: Tsukamurella ocularis sp. nov. and Tsukamurella hominis sp. nov., isolated from patients with conjunctivitis in Hong Kong. IJSEM 68: 810 - 818 2018 ( DOI 10.1099/ijsem.0.002589 , PubMed 29458478 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Tsukamurella ocularis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF