Name: Aeribacillus composti N.8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158560

Type strain:

Species: Aeribacillus composti

Strain Designation: N.8

Culture col. no.: KCTC 33824, JCM 31580

Strain history: <- Annarita Poli, Consiglio Nazionale delle Ricerche(CNR)

NCBI tax ID(s): 1868734 (species)

Section

Aeribacillus composti N.8 is an obligate aerobe, haloalkaliphilic, spore-forming bacterium that forms circular colonies and was isolated from compost sample.

colony-forming
Gram-positive
rod-shaped
spore-forming
haloalkaliphilic
obligate aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Aeribacillus*
	Species	**Aeribacillus composti**
	Full Scientific Name (LPSN)	Aeribacillus composti Finore et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65257]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #65257]	Cell length	1.5-5 µm

[Ref.: #65257]	Cell width	0.5-0.6 µm

[Ref.: #65257]	Cell shape	rod-shaped

[Ref.: #65257]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %92.369

[Ref.: #65257]	Colony size	1 mm
[Ref.: #65257]	Colony color	cream
[Ref.: #65257]	Colony shape	circular
[Ref.: #65257]	Incubation period	1 day
[Ref.: #65257]	Cultivation medium used	solid medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65257]

Culture medium

solid medium

[Ref.: #65257]

Culture medium growth

[Ref.: #65257]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65257]	growth	50-65 °C
[Ref.: #65257]	optimum	60 °C
[Ref.: #67770]	growth	55 °C
[Ref.: #67771]	growth	60 °C

[Ref.: #65257]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #65257]			optimum	9
[Ref.: #65257]			growth	5.5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65257]	Oxygen tolerance	obligate aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #65257]	Type of spore	endospore
[Ref.: #65257]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.999
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

[Ref.: #65257]	Halophily / tolerance level	haloalkaliphilic
[Ref.: #65257]	Halophily / tolerance level	haloalkaliphilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65257]		NaCl		growth	2-9	%(w/v)
[Ref.: #65257]		NaCl		optimum	6	%(w/v)

[Ref.: #65257]	Observation	Positive for aminopeptidase test.
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65257]	(S)-lactate ChEBI	+	carbon source
[Ref.: #65257]	2-deoxy-d-ribose ChEBI	+	carbon source
[Ref.: #65257]	4-hydroxy-L-proline ChEBI	+	nitrogen source
[Ref.: #65257]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #65257]	casein	-	hydrolysis
[Ref.: #65257]	D-arabinose ChEBI	+	assimilation
[Ref.: #65257]	D-glucose ChEBI	-	builds gas from
[Ref.: #65257]	D-glucose ChEBI	+	builds acid from
[Ref.: #65257]	D-glucose ChEBI	+	carbon source
[Ref.: #65257]	D-mannitol ChEBI	+	carbon source
[Ref.: #65257]	D-mannose ChEBI	+	carbon source
[Ref.: #65257]	D-ribose ChEBI	+	carbon source
[Ref.: #65257]	D-sorbitol ChEBI	+	carbon source
[Ref.: #65257]	D-xylose ChEBI	+	carbon source
[Ref.: #65257]	fumarate ChEBI	+	carbon source
[Ref.: #65257]	gelatin ChEBI	-	hydrolysis
[Ref.: #65257]	glycerol ChEBI	+	carbon source
[Ref.: #65257]	glycine ChEBI	+	nitrogen source
[Ref.: #65257]	hippurate ChEBI	+	hydrolysis
[Ref.: #65257]	L-alanine ChEBI	+	nitrogen source
[Ref.: #65257]	L-arabinose ChEBI	+	assimilation
[Ref.: #65257]	L-arginine ChEBI	+	nitrogen source
[Ref.: #65257]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #65257]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #65257]	L-fucose ChEBI	+	carbon source
[Ref.: #65257]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #65257]	L-glutamine ChEBI	+	nitrogen source
[Ref.: #65257]	L-histidine ChEBI	+	nitrogen source
[Ref.: #65257]	L-isoleucine ChEBI	+	nitrogen source
[Ref.: #65257]	L-leucine ChEBI	+	nitrogen source
[Ref.: #65257]	L-lysine ChEBI	+	nitrogen source
[Ref.: #65257]	L-methionine ChEBI	+	nitrogen source
[Ref.: #65257]	L-ornithine ChEBI	+	nitrogen source
[Ref.: #65257]	L-phenylalanine ChEBI	+	nitrogen source
[Ref.: #65257]	L-rhamnose ChEBI	+	carbon source
[Ref.: #65257]	L-serine ChEBI	+	nitrogen source
[Ref.: #65257]	L-threonine ChEBI	+	nitrogen source
[Ref.: #65257]	L-tryptophan ChEBI	+	nitrogen source
[Ref.: #65257]	L-tyrosine ChEBI	+	hydrolysis
[Ref.: #65257]	L-tyrosine ChEBI	+	nitrogen source
[Ref.: #65257]	L-valine ChEBI	+	nitrogen source
[Ref.: #65257]	maltose ChEBI	+	carbon source
[Ref.: #65257]	maltotriose ChEBI	+	carbon source
[Ref.: #65257]	phenylalanine ChEBI	-	hydrolysis
[Ref.: #65257]	pyruvate ChEBI	+	carbon source
[Ref.: #65257]	starch ChEBI	-	hydrolysis
[Ref.: #65257]	succinate ChEBI	+	carbon source
[Ref.: #65257]	sucrose ChEBI	+	carbon source
[Ref.: #65257]	trehalose ChEBI	+	carbon source
[Ref.: #65257]	urea ChEBI	-	hydrolysis
[Ref.: #65257]	xylan ChEBI	-	hydrolysis
[Ref.: #65257]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65257]	ampicillin ChEBI	yes	25	µg (disc)
[Ref.: #65257]	bacitracin ChEBI	yes	10	Unit
[Ref.: #65257]	chloramphenicol ChEBI	yes	10	µg (disc)
[Ref.: #65257]	erythromycin ChEBI	yes	30	µg (disc)
[Ref.: #65257]	fusidate ChEBI	yes	10	µg (disc)
[Ref.: #65257]	gentamicin ChEBI	yes	30	µg (disc)
[Ref.: #65257]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #65257]	lincomycin ChEBI	yes	15	µg (disc)
[Ref.: #65257]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #65257]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #65257]	nystatin ChEBI				yes	100	µg (disc)
[Ref.: #65257]	penicillin ChEBI	yes	10	Unit
[Ref.: #65257]	streptomycin ChEBI	yes	25	µg (disc)
[Ref.: #65257]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #65257]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65257]		acetoin ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #65257]		acetoin ChEBI	-

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65257]		catalase	+	1.11.1.6
[Ref.: #65257]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65257]	Sample type/isolated from	compost sample
[Ref.: #65257]	Geographic location (country and/or sea, region)	Composting Experimental Centre (CESCO) in Cilento National Park, Salerno
[Ref.: #65257]	Country	Italy
[Ref.: #65257]	Country ISO 3 Code	ITA
[Ref.: #65257]	Continent	Europe
[Ref.: #65257]	Geographic location	40.2885°/15.3608°
[Ref.: #65257]	Enrichment culture	growth medium A
[Ref.: #65257]	Enrichment culture composition	(l-1): peptone, 8 g; yeast extract 4 g and NaCl, 2 g
[Ref.: #65257]	Enrichment culture temperature	60 ̊C
[Ref.: #65257]	Isolation/enrichment procedure	The compost sample (1 g) was dissolved in 200 ml sterilised 50 mM phosphate buffer (pH 7.0) with agitation (120 r.p.m.) for 24 h at 60°C. The suspension obtained was used to inoculate 10 ml growth medium A. After 48 h at 60°C, the growth that had occurred was used as an inoculum for the same solid medium obtained by adding agar [1.8 % (w/v)]. After 48 h incubation at 60°C growth had occurred and colonies were purified by using the serial dilution-plating method at 60°C followed by restreaking on

[Ref.: #67770]	Sample type/isolated from	Curing step of an olive mill pomace compost sample collected at the Composting Experimental Centre
[Ref.: #67770]	Geographic location (country and/or sea, region)	Salerno
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe

[Ref.: #67771]	Sample type/isolated from	From compost
[Ref.: #67771]	Geographic location (country and/or sea, region)	Laurino, Salerno-Experimental Composting Center
[Ref.: #67771]	Country	Italy
[Ref.: #67771]	Country ISO 3 Code	ITA
[Ref.: #67771]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Biodegradation

#Composting

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT594972 (>99% sequence identity) for Aeribacillus from Microbeatlas

Aquatic Samples	1682
Soil Samples	1959
Animal Samples	5491
Plant Samples	654
Total Samples	9786

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65257]	16S rRNA gene sequence	LT594972		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Aeribacillus composti KCTC 33824	GCA_007827555	scaffold	GenBank	1868734 tax ID	*
[Ref.: #66792]	Aeribacillus composti strain KCTC 33824	1868734.3	wgs	PATRIC	1868734 tax ID	*
[Ref.: #66792]	Aeribacillus composti KCTC 33824	2802428823	draft	JGI IMG/M	1868734 tax ID	*

[Ref.: #65257]

GC-content

40.5 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Aeribacillus composti KCTC 33824 NCBI: GCA_007827555.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Aeribacillus composti KCTC 33824 NCBI: GCA_007827555.1
[Ref.: #69480]	motile	yes	83.148	yes
[Ref.: #69480]	flagellated	yes	89.617	no
[Ref.: #69480]	gram-positive	yes	80.895	no
[Ref.: #69480]	anaerobic	no	96.54	no
[Ref.: #69480]	aerobic	yes	88.956	yes
[Ref.: #69480]	halophile	no	72.203	no
[Ref.: #69480]	spore-forming	yes	96.24	yes
[Ref.: #69480]	glucose-util	yes	85.628	no
[Ref.: #69480]	thermophile	yes	94.209	yes
[Ref.: #69480]	glucose-ferment	no	93.662	no

Availability in culture collections External links

[Ref.: #65257]

Culture collection no.

KCTC 33824, JCM 31580

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Aeribacillus composti sp. nov., a thermophilic bacillus isolated from olive mill pomace compost.	Finore I, Gioiello A, Leone L, Orlando P, Romano I, Nicolaus B, Poli A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002391	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65257

Ilaria Finore, Alessia Gioiello, Luigi Leone, Pierangelo Orlando, Ida Romano, Barbara Nicolaus, Annarita Poli: Aeribacillus composti sp. nov., a thermophilic bacillus isolated from olive mill pomace compost. IJSEM 67: 4830 - 4835 2017 ( DOI 10.1099/ijsem.0.002391 , PubMed 28984237 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Aeribacillus composti KCTC 33824

Aeribacillus composti KCTC 33824

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