Name: Rhodoplanes tepidicaeni TUT3542
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158541

Type strain:

Species: Rhodoplanes tepidicaeni

Strain Designation: TUT3542

Culture col. no.: KCTC 15602, NBRC 112815

Strain history: <- Akira Hiraishi, Toyohashi Univ.

NCBI tax ID(s): 1897629 (species)

Section

Rhodoplanes tepidicaeni TUT3542 is a microaerophile, diazotroph, Gram-negative bacterium that was isolated from Sediment mud and cyanobacterial mat samples.

Gram-negative
motile
rod-shaped
diazotroph
microaerophile
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Nitrobacteraceae*
	Genus	*Rhodoplanes*
	Species	**Rhodoplanes tepidicaeni**
	Full Scientific Name (PNU)	Rhodoplanes tepidicaeni Hiraishi 2017

Information on morphological and physiological properties Morphology

[Ref.: #65231]	Gram stain	negative
[Ref.: #65231]	Cell length	1.5-3.5 µm
[Ref.: #65231]	Cell width	0.9 µm
[Ref.: #65231]	Cell shape	rod-shaped
[Ref.: #65231]	Motility	yes
[Ref.: #65231]	Flagellum arrangement	monotrichous, polar

[Ref.: #67771]	Gram stain	negative

[Ref.: #67771]	Cell shape	rod-shaped
[Ref.: #67771]	Motility	yes
[Ref.: #67771]	Flagellum arrangement	monotrichous, polar

[Ref.: #65231]

Colony color

pink

[Ref.: #65231]	Pigment production	yes
[Ref.: #65231]	Pigment name	Bacteriochlorophyll a
[Ref.: #65231]	Pigment production	yes
[Ref.: #65231]	Pigment name	carotenoids of the spirilloxanthin series

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #65231]	growth	25-45 °C
[Ref.: #65231]	optimum	40 °C
[Ref.: #67771]	growth	35 °C

[Ref.: #65231]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65231]			optimum	6.8-7.2
[Ref.: #65231]			growth	5.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

microaerophile

[Ref.: #65231]

Nutrition type

diazotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65231]		NaCl		growth	0-1.5	%
[Ref.: #65231]		NaCl	no	growth	>2	%

[Ref.: #67771]

Observation

quinones: Q-10, RQ-10, Q-9

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65231]	acetate ChEBI	+	carbon source
[Ref.: #65231]	arginine ChEBI	-	carbon source
[Ref.: #65231]	aspartate ChEBI	-	carbon source
[Ref.: #65231]	benzoate ChEBI	-	carbon source
[Ref.: #65231]	beta-alanine ChEBI	-	carbon source
[Ref.: #65231]	butyrate ChEBI	-	carbon source
[Ref.: #65231]	casamino acids	+	carbon source
[Ref.: #65231]	citrate ChEBI	+	carbon source
[Ref.: #65231]	D-fructose ChEBI	-	carbon source
[Ref.: #65231]	D-glucose ChEBI	-	carbon source
[Ref.: #65231]	D-mannitol ChEBI	-	carbon source
[Ref.: #65231]	D-mannose ChEBI	-	carbon source
[Ref.: #65231]	D-sorbitol ChEBI	-	carbon source
[Ref.: #65231]	D-xylose ChEBI	-	carbon source
[Ref.: #65231]	decanoate ChEBI	-	carbon source
[Ref.: #65231]	formate ChEBI	-	carbon source
[Ref.: #65231]	fumarate ChEBI	+	carbon source
[Ref.: #65231]	gluconate ChEBI	+	carbon source
[Ref.: #65231]	glutamate ChEBI	-	carbon source
[Ref.: #65231]	glutamate ChEBI	-	nitrogen source
[Ref.: #65231]	glutamine ChEBI	-	carbon source
[Ref.: #65231]	glutamine ChEBI	+	nitrogen source
[Ref.: #65231]	glycerol ChEBI	-	carbon source
[Ref.: #65231]	glycolate ChEBI	-	carbon source
[Ref.: #65231]	L-arabinose ChEBI	-	carbon source
[Ref.: #65231]	lactate ChEBI	+	carbon source
[Ref.: #65231]	lactose ChEBI	-	carbon source
[Ref.: #65231]	malate ChEBI	+	carbon source
[Ref.: #65231]	malonate ChEBI	+	carbon source
[Ref.: #65231]	methanol ChEBI	-	carbon source
[Ref.: #65231]	methionine ChEBI	-	carbon source
[Ref.: #65231]	nitrate ChEBI	+	nitrogen source
[Ref.: #65231]	nonanoate ChEBI	-	carbon source
[Ref.: #65231]	octanoate ChEBI	-	carbon source
[Ref.: #65231]	peptone	+	carbon source
[Ref.: #65231]	propionate ChEBI	-	carbon source
[Ref.: #65231]	pyruvate ChEBI	+	carbon source
[Ref.: #65231]	succinate ChEBI	+	carbon source
[Ref.: #65231]	sucrose ChEBI	-	carbon source
[Ref.: #65231]	sulfate ChEBI	-	sulfur source
[Ref.: #65231]	tartrate ChEBI	+	carbon source
[Ref.: #65231]	thiosulfate ChEBI	+	sulfur source
[Ref.: #65231]	urea ChEBI	+	nitrogen source
[Ref.: #65231]	valerate ChEBI	-	carbon source
[Ref.: #65231]	yeast extract	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65231]	Sample type/isolated from	Sediment mud and cyanobacterial mat samples
[Ref.: #65231]	Geographic location (country and/or sea, region)	Nakanoyu hot spring, Azumi district, Matsumoto, Nagano Prefecture
[Ref.: #65231]	Country	Japan
[Ref.: #65231]	Country ISO 3 Code	JPN
[Ref.: #65231]	Continent	Asia
[Ref.: #65231]	Geographic location	36.2069°/137.6060°
[Ref.: #65231]	Enrichment culture	Rhodoplanes (RPL) medium
[Ref.: #65231]	Enrichment culture composition	10 ml of Rhodoplanes (RPL) medium (mineral base +0.1 % each of sodium pyruvate, sodium succinate and yeast extract+0.05 % Na2S2O3 x 5H2O + vitamin B12, pH 6.8)
[Ref.: #65231]	Enrichment culture duration	2 weeks
[Ref.: #65231]	Enrichment culture temperature	42 and 50 ̊C
[Ref.: #65231]	Isolation/enrichment procedure	Small portions of the samples were introduced into 20 ml screw-capped test tubes containing 10 ml of Rhodoplanes (RPL) medium (mineral base +0.1 % each of sodium pyruvate, sodium succinate and yeast extract+0.05 % Na2S2O3 x 5H 2 O + vitamin B12, pH 6.8), filled completely with the same medium and incubated at 42°C and 50°C under incandescent illumination at 20 W m^-2. While incubation at 50°C resulted in the enrichment of orange-green Chloroflexus cultures, pink cultures were obtained at 42°C fr

[Ref.: #67771]	Sample type/isolated from	From hot spring mud
[Ref.: #67771]	Geographic location (country and/or sea, region)	Nakanoyu hot spring, Azumi, Matsumoto, Nagano Prefecture
[Ref.: #67771]	Country	Japan
[Ref.: #67771]	Country ISO 3 Code	JPN
[Ref.: #67771]	Continent	Asia
[Ref.: #67771]	Geographic location	36.2069°/137.6060°
* marker position based on {}

Isolation sources categories

#Environmental	#Microbial community	#Microbial mat
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

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Global distribution of 16S sequence LC178581 (>99% sequence identity) for Rhodoplanes from Microbeatlas

Aquatic Samples	22
Soil Samples	18
Animal Samples	3
Plant Samples	3
Total Samples	46

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65231]	16S rRNA gene sequence	LC178581		GenBank

[Ref.: #65231]

GC-content

67.7 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65231]

Culture collection no.

KCTC 15602, NBRC 112815

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of thermotolerant phototrophic bacteria, Rhodoplanes tepidicaeni sp. nov. and Rhodoplanes azumiensis sp. nov., isolated from a geothermal spring.	Hiraishi A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002408	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65231

Akira Hiraishi: Characterization of thermotolerant phototrophic bacteria, Rhodoplanes tepidicaeni sp. nov. and Rhodoplanes azumiensis sp. nov., isolated from a geothermal spring. IJSEM 37: 5038 - 5045 2017 ( DOI 10.1099/ijsem.0.002408 , PubMed 29034848 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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