Name: Flavobacterium amniphilum KYPY10
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158535

Type strain:

Species: Flavobacterium amniphilum

Strain Designation: KYPY10

Culture col. no.: BCRC 81006, LMG 29727, KCTC 52443

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

NCBI tax ID(s): 1834035 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Flavobacterium amniphilum KYPY10 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from water sample.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Flavobacterium*
	Species	**Flavobacterium amniphilum**
	Full Scientific Name (LPSN)	Flavobacterium amniphilum Sheu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65223]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %98.891

[Ref.: #65223]	Cell length	2.5-3.0 µm

[Ref.: #65223]	Cell width	0.2-0.3 µm

[Ref.: #65223]	Cell shape	rod-shaped

[Ref.: #65223]	Motility	yes
[Ref.: #125438]	Motility	no Confidence in %94

[Ref.: #65223]	Flagellum arrangement	gliding

[Ref.: #65223]	Colony size	1.0-1.8 mm
[Ref.: #65223]	Colony color	light yellow
[Ref.: #65223]	Colony shape	circular
[Ref.: #65223]	Incubation period	2 days
[Ref.: #65223]	Cultivation medium used	R2A

[Ref.: #65223]	Pigment production	no
[Ref.: #65223]	Pigment name	flexirubin-type pigments

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

tripticase soy agar

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Culture medium growth

		Temperature
[Ref.: #65223]	growth	20-30 °C
[Ref.: #65223]	optimum	25 °C
[Ref.: #67771]	growth	25 °C

	pH	Kind of pH		pH
[Ref.: #65223]			optimum	6
[Ref.: #65223]			growth	6-7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65223]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65223]		NaCl		growth	0-0.2	%(w/v)
[Ref.: #65223]		NaCl		optimum	0	%(w/v)

[Ref.: #65223]	Observation	colonies do not adhere to the agar
[Ref.: #65223]	Observation	Congo red is not absorbed by colonies

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65223]	2-hydroxybutyrate ChEBI	+	respiration
[Ref.: #65223]	adipate ChEBI	-	growth
[Ref.: #65223]	alpha-cyclodextrin ChEBI	+	respiration
[Ref.: #65223]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #65223]	casein	+	hydrolysis
[Ref.: #65223]	chitin ChEBI	-	hydrolysis
[Ref.: #65223]	D-glucose ChEBI	-	builds acid from
[Ref.: #65223]	D-glucose ChEBI	-	growth
[Ref.: #65223]	D-mannitol ChEBI	-	growth
[Ref.: #65223]	D-mannose ChEBI	-	growth
[Ref.: #65223]	decanoate ChEBI	-	growth
[Ref.: #65223]	dna ChEBI	-	hydrolysis
[Ref.: #65223]	esculin ChEBI	-	hydrolysis
[Ref.: #65223]	gelatin ChEBI	+	hydrolysis
[Ref.: #65223]	glycogen ChEBI	+	respiration
[Ref.: #65223]	L-arabinose ChEBI	-	growth
[Ref.: #65223]	L-glutamate ChEBI	+	respiration
[Ref.: #65223]	L-proline ChEBI	+	respiration
[Ref.: #65223]	L-tyrosine ChEBI	+	hydrolysis
[Ref.: #65223]	lecithin ChEBI	-	hydrolysis
[Ref.: #65223]	malate ChEBI	-	growth
[Ref.: #65223]	maltose ChEBI	-	growth
[Ref.: #65223]	maltose ChEBI	+	respiration
[Ref.: #65223]	N-acetylglucosamine ChEBI	-	growth
[Ref.: #65223]	nitrate ChEBI	-	reduction
[Ref.: #65223]	pectin ChEBI	-	hydrolysis
[Ref.: #65223]	phenylacetate ChEBI	-	growth
[Ref.: #65223]	potassium gluconate ChEBI	-	growth
[Ref.: #65223]	sodium citrate ChEBI	-	growth
[Ref.: #65223]	starch ChEBI	+	hydrolysis
[Ref.: #65223]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65223]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65223]	tween 60 ChEBI	+	hydrolysis
[Ref.: #65223]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65223]	uridine ChEBI	+	respiration
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #65223]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65223]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #65223]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #65223]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #65223]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #65223]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #65223]	penicillin g ChEBI	yes	10	Unit (disc)
[Ref.: #65223]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #65223]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #65223]	tetracycline ChEBI	yes	30	µg (disc)

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #65223]		26	sulfamethoxazole ChEBI	yes	23.75	µg (disc)
[Ref.: #65223]		26	trimethoprim ChEBI	yes	1.25	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #65223]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65223]	acid phosphatase	+	3.1.3.2
[Ref.: #65223]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65223]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65223]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65223]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65223]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65223]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65223]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65223]	beta-galactosidase	-	3.2.1.23
[Ref.: #65223]	beta-glucosidase	-	3.2.1.21
[Ref.: #65223]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65223]	catalase	+	1.11.1.6
[Ref.: #65223]	cystine arylamidase	+	3.4.11.3
[Ref.: #65223]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65223]	esterase (C 4)	+
[Ref.: #65223]	esterase Lipase (C 8)	+
[Ref.: #65223]	leucine arylamidase	+	3.4.11.1
[Ref.: #65223]	lipase	-
[Ref.: #65223]	lipase (C 14)	-
[Ref.: #65223]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65223]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65223]	trypsin	-	3.4.21.4
[Ref.: #65223]	urease	-	3.5.1.5
[Ref.: #65223]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65223]	Sample type/isolated from	water sample
[Ref.: #65223]	Geographic location (country and/or sea, region)	Funglin Stream in the vicinity of Hualien County
[Ref.: #65223]	Country	Taiwan, Province of China
[Ref.: #65223]	Country ISO 3 Code	TWN
[Ref.: #65223]	Continent	Asia
[Ref.: #65223]	Geographic location	23.7594°/121.4470°
[Ref.: #65223]	Enrichment culture	R2A agar
[Ref.: #65223]	Enrichment culture duration	3 days
[Ref.: #65223]	Enrichment culture temperature	25 ̊C
[Ref.: #65223]	Isolation/enrichment procedure	aerobic incubation

[Ref.: #67771]	Sample type/isolated from	From river
[Ref.: #67771]	Geographic location (country and/or sea, region)	Hualien County
[Ref.: #67771]	Country	Taiwan, Province of China
[Ref.: #67771]	Country ISO 3 Code	TWN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65223]	Flavobacterium sp. KYPY10 partial 16S rRNA gene, strain KYPY10	LT555403		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Flavobacterium amniphilum KYPY10	GCA_023634845	scaffold	GenBank ENA	1834035 tax ID	*

[Ref.: #65223]

GC-content

41 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Flavobacterium amniphilum KYPY10 NCBI: GCA_023634845.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	98.891	yes
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	97.331	yes
[Ref.: #125438]	aerobic	aerobicⓘ	yes	83.515	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	89.744	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.388	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	94	yes

Availability in culture collections External links

[Ref.: #65223]

Culture collection no.

BCRC 81006, LMG 29727, KCTC 52443

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flavobacterium amnicola sp. nov., isolated from a sub-tropical stream.	Sheu SY, Guo YP, Chen WM	Int J Syst Evol Microbiol	10.1099/ijsem.0.003463	2019	*
Phylogeny	Flavobacterium amniphilum sp. nov., isolated from a stream.	Sheu SY, Su CL, Kwon SW, Chen WM	Int J Syst Evol Microbiol	10.1099/ijsem.0.002443	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65223

Shih-Yi Sheu, Ching-Ling Su, Soon-Wo Kwon, Wen-Ming Chen: Flavobacterium amniphilum sp. nov., isolated from a stream. IJSEM 67: 5179 - 5186 2017 ( DOI 10.1099/ijsem.0.002443 , PubMed 29072560 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

* These data were automatically processed and therefore are not curated

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