Name: Prevotella cerevisiae DSM 100619
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158507

Type strain:

Species: Prevotella cerevisiae

Culture col. no.: DSM 100619, JCM 30867, SBC 8034

Strain history: H. Kanda; Sapporo Breweries Ltd., Japan; SBC8034.

NCBI tax ID(s): 2053716 (species)

Other strains from Prevotella cerevisiae

P. cerevisiae DSM 100620, JCM 30868, SBC 8065

Section

Prevotella cerevisiae DSM 100619 is an obligate anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from brewery wastewater.

colony-forming
Gram-negative
rod-shaped
mesophilic
obligate anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Prevotella*
	Species	**Prevotella cerevisiae**
	Full Scientific Name (PNU)	Prevotella cerevisiae Nakata et al. 2019

Synonym

"Segatella cerevisiae"

Information on morphological and physiological properties Morphology

[Ref.: #67892]	Gram stain	negative
[Ref.: #67892]	Cell length	2-5 µm
[Ref.: #67892]	Cell width	0.8 µm
[Ref.: #67892]	Cell shape	rod-shaped
[Ref.: #67892]	Motility	no

[Ref.: #67892]	Colony size	0.5-1 mm
[Ref.: #67892]	Colony color	white to off-white brown
[Ref.: #67892]	Colony shape	circular
[Ref.: #67892]	Incubation period	5 days
[Ref.: #67892]	Cultivation medium used	MRS agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65183]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #65183]

Culture medium growth

[Ref.: #65183]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #65183]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #65183]			growth	30 °C
[Ref.: #67770]			growth	30 °C

[Ref.: #65183]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #67892]			optimum	5-7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67892]

Oxygen tolerance

obligate anaerobe

[Ref.: #67892]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67892]	arabinose ChEBI	+	fermentation
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #67892]	cellobiose ChEBI	+	fermentation
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #67892]	glucose ChEBI	+	fermentation
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #67892]	lactose ChEBI	+	fermentation
[Ref.: #67892]	maltose ChEBI	+	fermentation
[Ref.: #67892]	mannitol ChEBI	-	fermentation
[Ref.: #67892]	mannose ChEBI	+	fermentation
[Ref.: #67892]	melezitose ChEBI	-	fermentation
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #67892]	raffinose ChEBI	+	fermentation
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #67892]	rhamnose ChEBI	-	fermentation
[Ref.: #67892]	salicin ChEBI	+	fermentation
[Ref.: #67892]	sorbitol ChEBI	-	fermentation
[Ref.: #67892]	trehalose ChEBI	-	fermentation
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #67892]	xylose ChEBI	-	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #67892]		indole ChEBI
[Ref.: #68380]		indole ChEBI	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #67892]	acid phosphatase	+	3.1.3.2
[Ref.: #67892]	alanine arylamidase	+	3.4.11.2
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #67892]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #67892]	alpha-arabinosidase	+
[Ref.: #68380]	alpha-arabinosidase	+	3.2.1.55	* from API rID32A
[Ref.: #67892]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #67892]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #67892]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #67892]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #67892]	beta-galactosidase	+	3.2.1.23
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #67892]	beta-glucosidase	+	3.2.1.21
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #67892]	cystine arylamidase	-	3.4.11.3
[Ref.: #67892]	esterase (C 4)	-
[Ref.: #67892]	esterase Lipase (C 8)	-
[Ref.: #67892]	glutamate decarboxylase	-	4.1.1.15
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #67892]	leucyl glycin arylamidase	+	3.4.11.1
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #67892]	lipase (C 14)	-
[Ref.: #67892]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #67892]	serine arylamidase	-
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #67892]	trypsin	-	3.4.21.4
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #67892]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #67892]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #65183]	11202	-	-	+	+	-	+	+	+	-	+	-	+	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+/-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65183]	Sample type/isolated from	brewery wastewater
[Ref.: #65183]	Geographic location (country and/or sea, region)	Gunma prefecture
[Ref.: #65183]	Country	Japan
[Ref.: #65183]	Country ISO 3 Code	JPN
[Ref.: #65183]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Brewery wastewater
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #67892]	Sample type/isolated from	brewery wastewater
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Industrial wastewater
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC331291 (>99% sequence identity) for Prevotellaceae from Microbeatlas

Aquatic Samples	303
Soil Samples	62
Animal Samples	435
Plant Samples	84
Total Samples	884

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65183]	Prevotella cerevisiae JCM 30867 gene for 16S rRNA, partial sequence	LC331291	1413	ENA	2053716 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Prevotella cerevisiae DSM 100619	GCA_024054555	contig	GenBank	2053716 tax ID	*
[Ref.: #66792]	Streptococcus pneumoniae GPSC4 substr. ST2344	GCA_900060295	scaffold	PATRIC	1313 tax ID	*

[Ref.: #67770]

GC-content

45 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65183]

Culture collection no.

DSM 100619, JCM 30867, SBC 8034

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Prevotella cerevisiae sp. nov., beer-spoilage obligate anaerobic bacteria isolated from brewery wastewater.	Nakata H, Kanda H, Nakakita Y, Kaneko T, Tsuchiya Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.003393	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65183

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100619

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#67892

Hajime Nakata, Hajime Kanda, Yasukazu Nakakita, Takeshi Kaneko, Youichi Tsuchiya: Prevotella cerevisiae sp. nov., beer-spoilage obligate anaerobic bacteria isolated from brewery wastewater. IJSEM 69: 1789 - 1793 2019 ( DOI 10.1099/ijsem.0.003393 )

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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