Name: Actinomadura craniellae LHW63021
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158480

Type strain:

Species: Actinomadura craniellae

Strain Designation: LHW63021

Culture col. no.: DSM 106125, CCTCC AA 2018015

Strain history: <- L. Li, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dep. Department of Pharmacy, Shanghai, P.R. China; LHW63021

NCBI tax ID(s): 2231787 (species)

SI-ID 404996

DSM 106125

Section

Actinomadura craniellae LHW63021 is an aerobe, spore-forming, mesophilic bacterium that was isolated from marine sponge.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura craniellae**
	Full Scientific Name (LPSN)	Actinomadura craniellae Li et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67850]	Gram stain	positive

[Ref.: #67850]	Motility	no

[Ref.: #69296]	Colony color	Fawn brown (8007)
[Ref.: #69296]	Cultivation medium used	ISP 7

[Ref.: #69296]	Colony color	Khaki grey (7008)
[Ref.: #69296]	Cultivation medium used	suter without tyrosine

[Ref.: #69296]	Colony color	Light ivory (1015)
[Ref.: #69296]	Cultivation medium used	ISP 3

[Ref.: #69296]	Colony color	Light ivory (1015)
[Ref.: #69296]	Cultivation medium used	ISP 5

[Ref.: #69296]	Colony color	Oyster white (013)
[Ref.: #69296]	Cultivation medium used	ISP 4

[Ref.: #69296]	Colony color	Quartz grey (7039)
[Ref.: #69296]	Cultivation medium used	suter with tyrosine

[Ref.: #69296]	Colony color	Sand yellow (1002)
[Ref.: #69296]	Cultivation medium used	ISP 2

[Ref.: #69296]	Colony color	Sand yellow (1002)
[Ref.: #69296]	Cultivation medium used	ISP 6

[Ref.: #69296]	Pigment production	no
[Ref.: #69296]	Pigment name	Melanin
[Ref.: #69296]	Pigment production	no
[Ref.: #69296]	Pigment name	soluble pigment

[Ref.: #67850]	Multicellular complex forming ability	yes
[Ref.: #67850]	Multicellular complex color	white to light yellow
[Ref.: #67850]	Medium Name (multicellularity)	ISP 3
[Ref.: #67850]	Multicellular complex further description	substrat mycelium

[Ref.: #67850]	Multicellular complex forming ability	yes
[Ref.: #67850]	Multicellular complex color	white
[Ref.: #67850]	Medium Name (multicellularity)	ISP 3
[Ref.: #67850]	Multicellular complex further description	aerial mycelia and curved spore-chains with 5-8 spherical spores (1.4 µm in diameter)

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	ISP 2

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	ISP 3

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	ISP 4

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	ISP 5

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	ISP 6

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	ISP 7

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69296]	Multicellular complex forming ability	no
[Ref.: #69296]	Multicellular complex name	Aerial mycelium
[Ref.: #69296]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #65156]

Caption:

Medium 535 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65156]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #65156]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #65156]

Culture medium composition

expand / minimize

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Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

[Ref.: #65156]

Culture medium growth

[Ref.: #65156]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65156]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #65156]			growth	28 °C
[Ref.: #67850]			growth	20-40 °C

[Ref.: #65156]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #67850]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67850]

Oxygen tolerance

aerobe

[Ref.: #67850]	Spore description	curved spore-chains with 5-8 spherical spores (1.4 µm in diameter)
[Ref.: #67850]	Type of spore	spore
[Ref.: #67850]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67850]		NaCl		growth	0-4	%
[Ref.: #69296]		NaCl		growth	0-2.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67850]	adenine ChEBI	-	nitrogen source
[Ref.: #69296]	arabinose ChEBI	-	growth
[Ref.: #67850]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #67850]	cellobiose ChEBI	-	carbon source
[Ref.: #69296]	cellulose ChEBI	-	growth
[Ref.: #67850]	D-arabinose ChEBI	-	carbon source
[Ref.: #67850]	D-fructose ChEBI	-	carbon source
[Ref.: #67850]	D-galactose ChEBI	-	carbon source
[Ref.: #67850]	D-glucose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67850]	D-mannitol ChEBI	-	carbon source
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #67850]	D-mannose ChEBI	-	carbon source
[Ref.: #67850]	D-ribose ChEBI	-	carbon source
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67850]	D-sorbitol ChEBI	-	carbon source
[Ref.: #67850]	D-xylose ChEBI	-	carbon source
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67850]	esculin ChEBI	-	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69296]	fructose ChEBI	-	growth
[Ref.: #67850]	galactitol ChEBI	-	carbon source
[Ref.: #67850]	gelatin ChEBI	-	hydrolysis
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69296]	glucose ChEBI	+	growth
[Ref.: #67850]	glycerol ChEBI	-	carbon source
[Ref.: #67850]	glycine ChEBI	+	nitrogen source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #67850]	hydroxyproline ChEBI	-	nitrogen source
[Ref.: #67850]	hypoxanthine ChEBI	-	nitrogen source
[Ref.: #69296]	inositol ChEBI	-	growth
[Ref.: #67850]	L-alanine ChEBI	-	nitrogen source
[Ref.: #67850]	L-arabinose ChEBI	-	carbon source
[Ref.: #67850]	L-arginine ChEBI	-	nitrogen source
[Ref.: #67850]	L-aspartate ChEBI	-	nitrogen source
[Ref.: #67850]	L-cysteine ChEBI	-	nitrogen source
[Ref.: #67850]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #67850]	L-glutamine ChEBI	-	nitrogen source
[Ref.: #67850]	L-histidine ChEBI	+	nitrogen source
[Ref.: #67850]	L-leucine ChEBI	-	nitrogen source
[Ref.: #67850]	L-lysine ChEBI	-	nitrogen source
[Ref.: #67850]	L-methionine ChEBI	-	nitrogen source
[Ref.: #67850]	L-phenylalanine ChEBI	-	nitrogen source
[Ref.: #67850]	L-proline ChEBI	+	nitrogen source
[Ref.: #67850]	L-serine ChEBI	+	nitrogen source
[Ref.: #67850]	L-threonine ChEBI	-	nitrogen source
[Ref.: #67850]	L-tryptophan ChEBI	-	nitrogen source
[Ref.: #67850]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #67850]	L-valine ChEBI	-	nitrogen source
[Ref.: #67850]	lactose ChEBI	-	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67850]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69296]	mannose ChEBI	-	growth
[Ref.: #67850]	melibiose ChEBI	-	carbon source
[Ref.: #67850]	milk	-	assimilation
[Ref.: #67850]	myo-inositol ChEBI	-	carbon source
[Ref.: #67850]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69296]	raffinose ChEBI	-	growth
[Ref.: #69296]	rhamnose ChEBI	+	growth
[Ref.: #67850]	sodium acetate ChEBI	-	carbon source
[Ref.: #67850]	sodium citrate ChEBI	-	carbon source
[Ref.: #67850]	sodium pyruvate ChEBI	+	carbon source
[Ref.: #67850]	starch ChEBI	-	hydrolysis
[Ref.: #67850]	sucrose ChEBI	-	carbon source
[Ref.: #69296]	sucrose ChEBI	-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67850]	trehalose ChEBI	+	carbon source
[Ref.: #67850]	tween 20 ChEBI	+	hydrolysis
[Ref.: #67850]	tween 40 ChEBI	+	hydrolysis
[Ref.: #67850]	tween 60 ChEBI	+	hydrolysis
[Ref.: #67850]	tween 80 ChEBI	+	hydrolysis
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #67850]	xanthine ChEBI	-	nitrogen source
[Ref.: #67850]	xylitol ChEBI	-	carbon source
[Ref.: #69296]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #67850]		hydrogen sulfide ChEBI	no
[Ref.: #67850]		melanin ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #67850]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #67850]

Fatty acid	Percentage
C16:0 iso	27
C18:0 iso	11.1
10-methyl C17:0	10.3
C18:1 w9c	10.2
C17:1 w8c	9.8
C16:0	7.3
C17:0	5.9
10-methyl C18:0, TBSA	5.9
C18:0	3.8
C19:1 w9c / C19:1 w11c	2.5
C16:1 w7c / C16:1 w6c	2.3
C16:1 iso G	1.7
10-methyl C19:0	1.2
C18:1 w7c	0.9

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69296]	3790	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69296]	5974	not determinedn.d.	+/-	+	+/-	+/-	+	+/-	-	-	-	-	+/-	-	-	-	+/-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65156]	Sample type/isolated from	marine sponge
[Ref.: #65156]	Geographic location (country and/or sea, region)	Xisha Islands, Hainan Province (16° 45' N, 112° 21' E)
[Ref.: #65156]	Country	China
[Ref.: #65156]	Country ISO 3 Code	CHN
[Ref.: #65156]	Continent	Asia
[Ref.: #65156]	Geographic location	16.7500°/112.3500°

[Ref.: #67850]	Sample type/isolated from	marine sponge sample, coral reef at 20m depth
[Ref.: #67850]	Geographic location (country and/or sea, region)	Xisha Islands, South China Sea
[Ref.: #67850]	Country	China
[Ref.: #67850]	Country ISO 3 Code	CHN
[Ref.: #67850]	Continent	Asia
[Ref.: #67850]	Geographic location	16.7500°/112.3500°
[Ref.: #67850]	Enrichment culture	Streptomyces isolation medium
[Ref.: #67850]	Enrichment culture composition	containing 3 % sea salt (w/v), 50 mg l-1 cycloheximide and 25 mg l-1 nalidixic acid.
[Ref.: #67850]	Enrichment culture duration	4-12 weeks
[Ref.: #67850]	Enrichment culture temperature	28 ̊C
[Ref.: #67850]	Isolation/enrichment procedure	After being washed in sterile artificial sea water three times, the tissue was cut, ground and spread on Streptomyces isolation medium and incubated at 28°C for 4-12 weeks.
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Invertebrates (Other)	#Porifera (Sponges)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67850]	Actinomadura craniellae 16S ribosomal RNA gene, partial sequence	MG200154	1433	ENA	2231787 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67850]	Actinomadura craniellae LHW63021	GCA_003289645	contig	GenBank	2231787 tax ID
[Ref.: #66792]	Actinomadura craniellae LHW63021	2856664274	draft	JGI IMG/M	2231787 tax ID	*

[Ref.: #67850]

GC-content

72 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #65156]

Culture collection no.

DSM 106125, CCTCC AA 2018015

[Ref.: #111276]

SI-ID 404996

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura craniellae sp. nov., isolated from a marine sponge in the South China Sea.	Li L, Xu QH, Wang XT, Lin HW, Lu YH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003295	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65156

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106125

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67850

Lei Li, Qi-hang Xu, Xiao-tian Wang, Hou-wen Lin, Yan-hua Lu: Actinomadura craniellae sp. nov., isolated from a marine sponge in the South China Sea. IJSEM 69: 1207 - 1212 2019 ( DOI 10.1099/ijsem.0.003295 )

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69296

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#111276

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404996.1 )

* These data were automatically processed and therefore are not curated

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