Name: Butyricimonas faecalis H184
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158474

Type strain:

Species: Butyricimonas faecalis

Strain Designation: H184

Culture col. no.: DSM 106867, JCM 33535, LMG 30602

Strain history: DSM 106867 <-- P. Cani; Univ. Catholique de Louvain, Louvain Drug Res. Inst., Belgium; H184 <-- T. Le Roy; Univ. Catholique de Louvain, Louvain Drug Res. Inst., Belgium.

NCBI tax ID(s): 2093856 (species)

SI-ID 399944

DSM 106867

Section

Butyricimonas faecalis H184 is an obligate anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from healthy human, stool sample.

colony-forming
Gram-negative
rod-shaped
mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Odoribacteraceae*
	Genus	*Butyricimonas*
	Species	**Butyricimonas faecalis**
	Full Scientific Name (LPSN)	Butyricimonas faecalis Le Roy et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67829]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.979

[Ref.: #67829]	Cell length	1.4-2 µm

[Ref.: #67829]	Cell width	0.4-0.5 µm

[Ref.: #67829]	Cell shape	rod-shaped

[Ref.: #67829]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.35

[Ref.: #67829]	Colony size	1-2 mm
[Ref.: #67829]	Colony color	beige
[Ref.: #67829]	Colony shape	circular
[Ref.: #67829]	Incubation period	3 days
[Ref.: #67829]	Cultivation medium used	solid modified YCFA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65150]

Culture medium

YCFA-MEDIUM (MODIFIED) (DSMZ Medium 1611)

[Ref.: #65150]

Culture medium growth

[Ref.: #65150]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65150]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65150]	growth	37 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #67829]	growth	25-42 °C
[Ref.: #67829]	optimum	37 °C

[Ref.: #65150]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67829]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #67829]			growth	5.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67829]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.132

[Ref.: #67829]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67829]		NaCl		growth	0-3.75	%
[Ref.: #67829]		NaCl		optimum	0-1.8	%

[Ref.: #67770]

Observation

quinones: MK-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67829]	cellobiose ChEBI	-	builds acid from
[Ref.: #67829]	D-arabinose ChEBI	-	builds acid from
[Ref.: #67829]	D-fructose ChEBI	-	builds acid from
[Ref.: #67829]	D-galactose ChEBI	+	builds acid from
[Ref.: #67829]	D-glucose ChEBI	+	builds acid from
[Ref.: #67829]	D-mannitol ChEBI	-	builds acid from
[Ref.: #67829]	D-mannose ChEBI	+	builds acid from
[Ref.: #67829]	D-ribose ChEBI	-	builds acid from
[Ref.: #67829]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #67829]	D-xylose ChEBI	-	builds acid from
[Ref.: #67829]	erythritol ChEBI	-	builds acid from
[Ref.: #67829]	esculin ChEBI	-	hydrolysis
[Ref.: #67829]	gelatin ChEBI	-	hydrolysis
[Ref.: #67829]	L-arabinose ChEBI	-	builds acid from
[Ref.: #67829]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #67829]	L-sorbose ChEBI	-	builds acid from
[Ref.: #67829]	L-xylose ChEBI	-	builds acid from
[Ref.: #67829]	lactose ChEBI	+	builds acid from
[Ref.: #67829]	maltose ChEBI	-	builds acid from
[Ref.: #67829]	melezitose ChEBI	-	builds acid from
[Ref.: #67829]	melibiose ChEBI	-	builds acid from
[Ref.: #67829]	myo-inositol ChEBI	-	builds acid from
[Ref.: #67829]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #67829]	nitrate ChEBI	-	reduction
[Ref.: #67829]	ribitol ChEBI	-	builds acid from
[Ref.: #67829]	sucrose ChEBI	-	builds acid from
[Ref.: #67829]	trehalose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #67829]		indole ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #67829]	alpha-fucosidase	+	3.2.1.51
[Ref.: #67829]	alpha-galactosidase	-	3.2.1.22
[Ref.: #67829]	arginine dihydrolase	+	3.5.3.6
[Ref.: #67829]	beta-galactosidase	+	3.2.1.23
[Ref.: #67829]	catalase	+	1.11.1.6
[Ref.: #67829]	gelatinase	-
[Ref.: #67829]	glutamate decarboxylase	+	4.1.1.15
[Ref.: #67829]	glutamyl-glutamate arylamidase	+
[Ref.: #67829]	leucyl glycin arylamidase	+	3.4.11.1
[Ref.: #67829]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #67829]	phenylalanine arylamidase	+
[Ref.: #67829]	pyroglutamic acid arylamidase	+
[Ref.: #67829]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65150]	Sample type/isolated from	healthy human, stool sample
[Ref.: #65150]	Geographic location (country and/or sea, region)	Bruxelles
[Ref.: #65150]	Country	Belgium
[Ref.: #65150]	Country ISO 3 Code	BEL
[Ref.: #65150]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Fecal sample of a healthy 31 year old female
[Ref.: #67770]	Country	Belgium
[Ref.: #67770]	Country ISO 3 Code	BEL
[Ref.: #67770]	Continent	Europe

[Ref.: #67829]	Sample type/isolated from	faecal sample of a healthy 31 year old female
[Ref.: #67829]	Enrichment culture	solid modified YCFA
[Ref.: #67829]	Isolation/enrichment procedure	The faecal sample was kept in a sealed container with an O2 -absorbing and CO2 - generating agent and isolation was performed within 2 h after collection. The samples were transferred into an anaerobic chamber (Coy) containing 100 % N2 as gas atmosphere and immediately diluted 1/10 in modified YCFA enriched in antioxidants. Faecal suspension was then transferred in tubes hermetically closed with butyl rubber under an atmosphere of 20 % CO2 - 80 % N2 . Then, single-cell cultivation was performed using extinction dilution technique, i.e. we diluted and aliquoted the faecal suspension in 300 vials such that a single vial received on average one cell. Positive cultures after 48 h to 7 days were spread onto solid modified YCFA and incubated 72 h to 7 days in anaerobic jars with an O2 -absorbing and CO2 -generating agent.
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65150]	Butyricimonas faecalis strain H184 16S ribosomal RNA gene, partial sequence	MG963286	1399	ENA	2093856 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67829]	Butyricimonas faecalis H184	GCA_003991565	complete	GenBank	2093856 tax ID
[Ref.: #66792]	Butyricimonas sp. H184	2093856.3	complete	PATRIC	2093856 tax ID	*

[Ref.: #67829]

GC-content

44.2 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Butyricimonas faecalis H184 NCBI: GCA_003991565.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Butyricimonas sp. H184 PATRIC: 2093856.3
[Ref.: #69480]	motile	no	90.78	no
[Ref.: #69480]	gram-positive	no	96.731	yes
[Ref.: #69480]	anaerobic	yes	98.927	yes
[Ref.: #69480]	aerobic	no	97.105	yes
[Ref.: #69480]	halophile	no	85.057	no
[Ref.: #69480]	spore-forming	no	92.439	no
[Ref.: #69480]	glucose-util	yes	86.224	no
[Ref.: #69480]	flagellated	no	97.365	yes
[Ref.: #69480]	thermophile	no	98.732	yes
[Ref.: #69480]	glucose-ferment	no	54.857	no

Availability in culture collections External links

[Ref.: #65150]

Culture collection no.

DSM 106867, JCM 33535, LMG 30602

[Ref.: #111271]

SI-ID 399944

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Butyricimonas faecalis sp. nov., isolated from human faeces and emended description of the genus Butyricimonas.	Le Roy T, Van der Smissen P, Paquot A, Delzenne N, Muccioli GG, Collet JF, Cani PD	Int J Syst Evol Microbiol	10.1099/ijsem.0.003249	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65150

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106867

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#67829

Tiphaine Le Roy, Patrick Van der Smissen, Adrien Paquot, Nathalie Delzenne, Giulio G. Muccioli, Jean-François Collet, Patrice D. Cani: Butyricimonas faecalis sp. nov., isolated from human faeces and emended description of the genus Butyricimonas. IJSEM 69: 833 - 839 2019 ( DOI 10.1099/ijsem.0.003249 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111271

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399944.1 )

* These data were automatically processed and therefore are not curated

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Butyricimonas faecalis H184

Butyricimonas sp. H184

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