[Ref.: #65113] |
Sample type/isolated from |
sediment from hydrothermal systems |
[Ref.: #65113] |
Geographic location (country and/or sea, region) |
Azores, Sao Miguel |
[Ref.: #65113] |
Country |
Portugal |
[Ref.: #65113] |
Country ISO 3 Code |
PRT |
[Ref.: #65113] |
Continent |
Europe |
|
[Ref.: #66748] |
Sample type/isolated from |
hydrothermal sediment samples |
[Ref.: #66748] |
Geographic location (country and/or sea, region) |
Sao Miguel Island, Azores |
[Ref.: #66748] |
Country |
Portugal |
[Ref.: #66748] |
Country ISO 3 Code |
PRT |
[Ref.: #66748] |
Continent |
Europe |
[Ref.: #66748] |
Geographic location |
37.7733°/-25.3036° |
[Ref.: #66748] |
Enrichment culture |
R2A agar |
[Ref.: #66748] |
Enrichment culture composition |
pH 6 |
[Ref.: #66748] |
Enrichment culture duration |
2-5 days |
[Ref.: #66748] |
Enrichment culture temperature |
60 ̊C |
[Ref.: #66748] |
Isolation/enrichment procedure |
Sediment samples of hydrothermal systems served as inoculum. The working electrode potential was set to 594 mV versus saturated calomel electrode and the ES medium (pH 3.5; 10 mM NH4Cl, 2.5 mM NaCl, 0.3 mM KH2PO4, 0.12 mM MgSO4, 0.1 mM CaCl2, 1 ml l-1 Wolfe's mineral elixir) was constantly sparged with a gas mix consisting of 80 % N2 and 20 % CO2 (v/v). The final enrichment was performed at 60°C under autotrophic conditions in ES medium with 0.25 cm^2 of the cathodic graphite felt as inoculum. The gas phase consisted of 20 % CO2, 75 % H2 and 5 % O2 (v/v/v). After serial dilutions in ES medium, single colonies were obtained by plating the culture on Reasoner's 2A agar at 60°C and 2-5 days. |
* marker position based on {}
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Isolation sources categories |
#Environmental |
#Aquatic |
#Hydrothermal vent |
#Environmental |
#Aquatic |
#Sediment |
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Availability in culture collections External links
[Ref.: #65113] |
Culture collection no. |
DSM 106492, CCOS 1194 |
[Ref.: #111238] |
SI-ID 397149
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* |
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Literature: |
|
Topic |
Title |
Authors |
Journal |
DOI |
Year |
|
Phylogeny |
Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at Sao Miguel Island, and emended description of the genus Kyrpidia. |
Reiner JE, Jung T, Lapp CJ, Siedler M, Bunk B, Overmann J, Gescher J |
Int J Syst Evol Microbiol |
10.1099/ijsem.0.003037 |
2018 |
* |
|
Improving the Cathodic Biofilm Growth Capabilities of Kyrpidia spormannii EA-1 by Undirected Mutagenesis. |
Jung T, Hackbarth M, Horn H, Gescher J |
Microorganisms |
10.3390/microorganisms9010077 |
2020 |
* |
|
- References
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-
#65113 |
Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ;
DSM 106492
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-
#66748 |
Johannes Eberhard Reiner, Tobias Jung, Christian Jonas Lapp, Marvin Siedler, Boyke Bunk, Jörg Overmann, Johannes Gescher:
Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at Sao Miguel Island, and emended description of the genus Kyrpidia.
IJSEM 68:
3735 - 3740
2018 (
DOI 10.1099/ijsem.0.003037 , PubMed 30234478 )
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#111238 |
Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.:
StrainInfo: A central database for resolving microbial strain identifiers
.
(
DOI 10.60712/SI-ID397149.1 )
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- * These data were automatically processed and therefore are not curated
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