The strain is capable of autotrophic growth with carbon dioxide as the sole carbon source using hydrogen as electron donor and oxygen as the terminal electron acceptor.
Sediment samples of hydrothermal systems served as inoculum. The working electrode potential was set to 594 mV versus saturated calomel electrode and the ES medium (pH 3.5; 10 mM NH4Cl, 2.5 mM NaCl, 0.3 mM KH2PO4, 0.12 mM MgSO4, 0.1 mM CaCl2, 1 ml l-1 Wolfe's mineral elixir) was constantly sparged with a gas mix consisting of 80 % N2 and 20 % CO2 (v/v). The final enrichment was performed at 60°C under autotrophic conditions in ES medium with 0.25 cm^2 of the cathodic graphite felt as inoculum. The gas phase consisted of 20 % CO2, 75 % H2 and 5 % O2 (v/v/v). After serial dilutions in ES medium, single colonies were obtained by plating the culture on Reasoner's 2A agar at 60°C and 2-5 days.
Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at Sao Miguel Island, and emended description of the genus Kyrpidia.
Reiner JE, Jung T, Lapp CJ, Siedler M, Bunk B, Overmann J, Gescher J
* These data were automatically processed and therefore are not curated
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