Name: Kyrpidia spormannii EA-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158437

Type strain:

Species: Kyrpidia spormannii

Strain Designation: EA-1

Culture col. no.: DSM 106492, CCOS 1194

Strain history: <- J. Gescher, Karlsruher Institut für Technologie, Germany; EA-1 <- J.E. Reiner

NCBI tax ID(s): 2055160 (species)

Other strains from Kyrpidia spormannii

K. spormannii FAVT 5, DSM 109470
K. spormannii COOX1, DSM 109471

Section

Kyrpidia spormannii EA-1 is an aerobe, spore-forming, thermophilic bacterium that was isolated from sediment from hydrothermal systems.

Gram-positive
rod-shaped
spore-forming
aerobe
thermophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Alicyclobacillaceae*
	Genus	*Kyrpidia*
	Species	**Kyrpidia spormannii**
	Full Scientific Name (PNU)	Kyrpidia spormannii Reiner et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66748]	Gram stain	positive
[Ref.: #66748]	Cell length	4-8 µm
[Ref.: #66748]	Cell width	0.7-1.2 µm
[Ref.: #66748]	Cell shape	rod-shaped
[Ref.: #66748]	Motility	no

[Ref.: #66748]	Colony color	pale cream
[Ref.: #66748]	Incubation period	2-5 days
[Ref.: #66748]	Cultivation medium used	Reasoner's 2A agar (R2A)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65113]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #65113]

Culture medium growth

[Ref.: #65113]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #65113]

Culture medium composition

expand / minimize

[Ref.: #66748]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #66748]

Culture medium growth

[Ref.: #66748]

Culture medium

Lysogeny broth medium

[Ref.: #66748]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65113]		growth	55 °C
[Ref.: #66748]	no	growth	37 °C
[Ref.: #66748]		optimum	55 °C
[Ref.: #66748]	no	growth	70 °C
[Ref.: #66748]		growth	45-65 °C

[Ref.: #65113]	Temperature range	thermophilic
[Ref.: #66748]	Temperature range	mesophilic
[Ref.: #66748]	Temperature range	thermophilic

	Kind of pH		pH
[Ref.: #66748]		optimum	5.5
[Ref.: #66748]	no	growth	<4.5
[Ref.: #66748]	no	growth	>7
[Ref.: #66748]		growth	4.5-7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66748]

Oxygen tolerance

aerobe

[Ref.: #66748]	Type of spore	endospore
[Ref.: #66748]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66748]		NaCl		growth	0-1	%(w/v)
[Ref.: #66748]		NaCl	no	growth	1.5	%(w/v)

[Ref.: #66748]	Observation	The strain is capable of autotrophic growth with carbon dioxide as the sole carbon source using hydrogen as electron donor and oxygen as the terminal electron acceptor.
[Ref.: #66748]	Observation	Nile red stains intracellular granules.
[Ref.: #66748]	Observation	Neither nitrate, DMSO, sulfate nor fumarate serves as electron acceptor under anaerobic growth conditions.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66748]	(R)-malic acid ChEBI	+	carbon source
[Ref.: #66748]	(S)-malic acid ChEBI	+	carbon source
[Ref.: #66748]	2-oxoglutarate ChEBI	+	carbon source
[Ref.: #66748]	acetic acid ChEBI	+	carbon source
[Ref.: #66748]	acetoacetate ChEBI	+	carbon source
[Ref.: #66748]	ammonium chloride ChEBI	+	nitrogen source
[Ref.: #66748]	asparagine ChEBI	+	nitrogen source
[Ref.: #66748]	carbon dioxide ChEBI	+	carbon source
[Ref.: #66748]	D-alanine ChEBI	+	carbon source
[Ref.: #66748]	D-fructose ChEBI	+	carbon source
[Ref.: #66748]	D-galactose ChEBI	+	carbon source
[Ref.: #66748]	D-galacturonic acid ChEBI	+	carbon source
[Ref.: #66748]	D-glucose ChEBI	+	carbon source
[Ref.: #66748]	D-mannose ChEBI	+	carbon source
[Ref.: #66748]	D-psicose ChEBI	+	carbon source
[Ref.: #66748]	D-ribose ChEBI	+	carbon source
[Ref.: #66748]	D-xylose ChEBI	+	carbon source
[Ref.: #66748]	dihydrogen ChEBI	+	electron donor
[Ref.: #66748]	dioxygen ChEBI	+	electron acceptor
[Ref.: #66748]	glucose ChEBI	-	fermentation
[Ref.: #66748]	glucuronamide ChEBI	+	carbon source
[Ref.: #66748]	L-arabinose ChEBI	+	carbon source
[Ref.: #66748]	L-fucose ChEBI	+	carbon source
[Ref.: #66748]	L-lyxose ChEBI	+	carbon source
[Ref.: #66748]	L-rhamnose ChEBI	+	carbon source
[Ref.: #66748]	monomethyl succinate ChEBI	+	carbon source
[Ref.: #66748]	pyruvate ChEBI	+	carbon source
[Ref.: #66748]	succinate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66748]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65113]	Sample type/isolated from	sediment from hydrothermal systems
[Ref.: #65113]	Geographic location (country and/or sea, region)	Azores, Sao Miguel
[Ref.: #65113]	Country	Portugal
[Ref.: #65113]	Country ISO 3 Code	PRT
[Ref.: #65113]	Continent	Europe

[Ref.: #66748]	Sample type/isolated from	hydrothermal sediment samples
[Ref.: #66748]	Geographic location (country and/or sea, region)	Sao Miguel Island, Azores
[Ref.: #66748]	Country	Portugal
[Ref.: #66748]	Country ISO 3 Code	PRT
[Ref.: #66748]	Continent	Europe
[Ref.: #66748]	Geographic location	37.7733°/-25.3036°
[Ref.: #66748]	Enrichment culture	R2A agar
[Ref.: #66748]	Enrichment culture composition	pH 6
[Ref.: #66748]	Enrichment culture duration	2-5 days
[Ref.: #66748]	Enrichment culture temperature	60 ̊C
[Ref.: #66748]	Isolation/enrichment procedure	Sediment samples of hydrothermal systems served as inoculum. The working electrode potential was set to 594 mV versus saturated calomel electrode and the ES medium (pH 3.5; 10 mM NH4Cl, 2.5 mM NaCl, 0.3 mM KH2PO4, 0.12 mM MgSO4, 0.1 mM CaCl2, 1 ml l-1 Wolfe's mineral elixir) was constantly sparged with a gas mix consisting of 80 % N2 and 20 % CO2 (v/v). The final enrichment was performed at 60°C under autotrophic conditions in ES medium with 0.25 cm^2 of the cathodic graphite felt as inoculum. The gas phase consisted of 20 % CO2, 75 % H2 and 5 % O2 (v/v/v). After serial dilutions in ES medium, single colonies were obtained by plating the culture on Reasoner's 2A agar at 60°C and 2-5 days.
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Kyrpidia spormannii EA-1	GCA_002804065	complete	GenBank	2055160 tax ID	*
[Ref.: #66792]	Kyrpidia spormannii EA-1	2775506848	complete	JGI IMG/M	2055160 tax ID	*

[Ref.: #65113]	GC-content	58.7 mol%
[Ref.: #66748]	GC-content	58.7 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #65113]

Culture collection no.

DSM 106492, CCOS 1194

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at Sao Miguel Island, and emended description of the genus Kyrpidia.	Reiner JE, Jung T, Lapp CJ, Siedler M, Bunk B, Overmann J, Gescher J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003037	2018	*
	Improving the Cathodic Biofilm Growth Capabilities of Kyrpidia spormannii EA-1 by Undirected Mutagenesis.	Jung T, Hackbarth M, Horn H, Gescher J	Microorganisms	10.3390/microorganisms9010077	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65113

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106492

#66748

Johannes Eberhard Reiner, Tobias Jung, Christian Jonas Lapp, Marvin Siedler, Boyke Bunk, Jörg Overmann, Johannes Gescher: Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at Sao Miguel Island, and emended description of the genus Kyrpidia. IJSEM 68: 3735 - 3740 2018 ( DOI 10.1099/ijsem.0.003037 , PubMed 30234478 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Kyrpidia spormannii in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF