Name: Megasphaera stantonii AJH120
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158428

Type strain:

Species: Megasphaera stantonii

Strain Designation: AJH120

Culture col. no.: DSM 106750, CCUG 71842

Strain history: <- T. Looft, USDA-ARS-NADC; AJH120 <- L. Lai

NCBI tax ID(s): 2144175 (species)

SI-ID 407607

DSM 106750, CCUG 71842

Special Collections

Section

Megasphaera stantonii AJH120 is an anaerobe, Gram-negative, coccus-shaped bacterium that forms circular colonies and was isolated from Healthy White Leghorn Chicken, cecum.

coccus-shaped
colony-forming
Gram-negative
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Megasphaera*
	Species	**Megasphaera stantonii**
	Full Scientific Name (LPSN)	Megasphaera stantonii Maki and Looft 2018

Information on morphological and physiological properties Morphology

[Ref.: #66708]	Gram stain	negative

[Ref.: #66708]	Cell length	0.8-1.8 µm

[Ref.: #66708]	Cell shape	coccus-shaped

[Ref.: #66708]	Motility	no

[Ref.: #66708]	Colony size	3.1-4 mm
[Ref.: #66708]	Colony color	white to yellow
[Ref.: #66708]	Colony shape	circular
[Ref.: #66708]	Incubation period	3 days
[Ref.: #66708]	Cultivation medium used	PYF

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65104]

Culture medium

PY + X MEDIUM (DSMZ Medium 104b)

[Ref.: #65104]

Culture medium growth

[Ref.: #65104]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65104]

Culture medium composition

expand / minimize

[Ref.: #66708]

Culture medium

peptone-yeast-fructose (PYF) medium

[Ref.: #66708]

Culture medium growth

[Ref.: #66708]

Culture medium composition

expand / minimize

[Ref.: #66708]

Culture medium

PYF

[Ref.: #66708]

Culture medium growth

		Temperature
[Ref.: #65104]	growth	40 °C
[Ref.: #66708]	optimum	42 °C
[Ref.: #66708]	growth	30-50 °C

	pH	Kind of pH		pH
[Ref.: #66708]			optimum	5.5-6.0
[Ref.: #66708]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66708]

Oxygen tolerance

anaerobe

[Ref.: #66708]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %93

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66708]	D-fructose ChEBI	+	assimilation
[Ref.: #66708]	D-fructose ChEBI	+	carbon source
[Ref.: #66708]	D-galactose ChEBI	-	assimilation
[Ref.: #66708]	D-gluconate ChEBI	+	carbon source
[Ref.: #66708]	D-glucose ChEBI	-	assimilation
[Ref.: #66708]	D-mannose ChEBI	-	assimilation
[Ref.: #66708]	D-ribose ChEBI	+	carbon source
[Ref.: #66708]	gluconate ChEBI	+	assimilation
[Ref.: #66708]	glycine ChEBI	+	carbon source
[Ref.: #66708]	L-alanine ChEBI	+	carbon source
[Ref.: #66708]	L-arabinose ChEBI	-	assimilation
[Ref.: #66708]	L-arginine ChEBI	+	carbon source
[Ref.: #66708]	L-isoleucine ChEBI	+	carbon source
[Ref.: #66708]	L-lysine ChEBI	+	carbon source
[Ref.: #66708]	L-phenylalanine ChEBI	+	carbon source
[Ref.: #66708]	L-proline ChEBI	+	carbon source
[Ref.: #66708]	L-serine ChEBI	+	carbon source
[Ref.: #66708]	L-tryptophan ChEBI	+	carbon source
[Ref.: #66708]	L-tyrosine ChEBI	+	carbon source
[Ref.: #66708]	lactate ChEBI	-	assimilation
[Ref.: #66708]	maltose ChEBI	-	assimilation
[Ref.: #66708]	mannitol ChEBI	-	assimilation
[Ref.: #66708]	pyruvate ChEBI	+	assimilation
[Ref.: #66708]	pyruvate ChEBI	+	carbon source
[Ref.: #66708]	succinate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #66708]	bacitracin ChEBI	yes	25	µg/mL
[Ref.: #66708]	chloramphenicol ChEBI	yes	25	µg/mL
[Ref.: #66708]	colistin ChEBI	yes	25	µg/mL
[Ref.: #66708]	erythromycin ChEBI	yes	25	µg/mL
[Ref.: #66708]	lincomycin ChEBI	yes	25	µg/mL
[Ref.: #66708]	penicillin ChEBI	yes	25	µg/mL
[Ref.: #66708]	streptomycin ChEBI				yes	27	µg/mL
[Ref.: #66708]	tetracycline ChEBI				yes	25	µg/mL
[Ref.: #66708]	vancomycin ChEBI				yes	28	µg/mL
[Ref.: #66708]	virginiamycin ChEBI				yes	26	µg/mL

	Metabolite	Production
[Ref.: #66708]	acetic acid ChEBI	yes
[Ref.: #66708]	formate ChEBI	yes
[Ref.: #66708]	phenylacetate ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66708]		catalase	-	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65104]	Sample type/isolated from	Healthy White Leghorn Chicken, cecum
[Ref.: #65104]	Geographic location (country and/or sea, region)	Iowa
[Ref.: #65104]	Country	USA
[Ref.: #65104]	Country ISO 3 Code	USA
[Ref.: #65104]	Continent	North America

[Ref.: #66708]	Sample type/isolated from	from the cecum of a healthy adult white leghorn chicken
[Ref.: #66708]	Enrichment culture	brain-heart infusion (BHI) medium (Difco)
[Ref.: #66708]	Enrichment culture composition	0.05 % (w/v) L-cysteine hydrochloride and 0.0001 % resazurin
[Ref.: #66708]	Enrichment culture duration	2 days
[Ref.: #66708]	Enrichment culture temperature	42 ̊C
[Ref.: #66708]	Isolation/enrichment procedure	brain-heart infusion (BHI) medium (Difco), supplemented with 0.05 % (w/v) L-cysteine hydrochloride and 0.0001 % resazurin. Cecal contents were diluted and plated before incubation for two days at 42C. C
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Gastrointestinal tract	#Large intestine
#Host	#Birds	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65104]	Megasphaera stantonii strain AJH120 16S ribosomal RNA gene, partial sequence	MG811574	1396	GenBank ENA	2144175 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Megasphaera stantonii AJH120	GCA_003367905	complete	GenBank ENA	2144175 tax ID	*
[Ref.: #66792]	Megasphaera stantonii AJH120	GCA_003030445	scaffold	GenBank ENA	2144175 tax ID	*
[Ref.: #66792]	Megasphaera stantonii AJH120	2841202678	complete	JGI IMG/M	2144175 tax ID	*

[Ref.: #65104]	GC-content	52.1 mol%
[Ref.: #66708]	GC-content	52.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Megasphaera stantonii AJH120 NCBI: GCA_003367905.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	93	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Megasphaera stantonii AJH120 NCBI: GCA_003367905.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	88.783	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	87.71	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	93.604	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	79.233	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.659	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	86.994	yes

Availability in culture collections External links

[Ref.: #65104]

Culture collection no.

DSM 106750, CCUG 71842

[Ref.: #111229]

SI-ID 407607

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Megasphaera stantonii AJH120(T), Isolated from a Chicken Cecum.	Maki JJ, Looft T	Microbiol Resour Announc	10.1128/MRA.01148-18	2018	*
Phylogeny	Megasphaera stantonii sp. nov., a butyrate-producing bacterium isolated from the cecum of a healthy chicken.	Maki JJ, Looft T	Int J Syst Evol Microbiol	10.1099/ijsem.0.002991	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65104

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106750

#66708

Joel J. Maki, Torey Looft: Megasphaera stantonii sp. nov., a butyrate-producing bacterium isolated from the cecum of a healthy chicken. IJSEM 68: 3409 - 3415 2018 ( DOI 10.1099/ijsem.0.002991 , PubMed 30281016 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111229

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407607.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Megasphaera stantonii in external resources:
SILVA
BRENDA
PANGAEA
GBIF