Name: Megasphaera stantonii AJH120
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158428

Type strain:

Species: Megasphaera stantonii

Strain Designation: AJH120

Culture col. no.: DSM 106750, CCUG 71842

Strain history: <- T. Looft, USDA-ARS-NADC; AJH120 <- L. Lai

NCBI tax ID(s): 2144175 (species)

SI-ID 407607

DSM 106750, CCUG 71842

Section

Megasphaera stantonii AJH120 is an anaerobe, thermophilic, Gram-negative bacterium that forms circular colonies and was isolated from Healthy White Leghorn Chicken, cecum.

coccus-shaped
colony-forming
Gram-negative
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Megasphaera*
	Species	**Megasphaera stantonii**
	Full Scientific Name (LPSN)	Megasphaera stantonii Maki and Looft 2018

Information on morphological and physiological properties Morphology

[Ref.: #66708]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.837

[Ref.: #66708]	Cell length	0.8-1.8 µm

[Ref.: #66708]	Cell shape	coccus-shaped

[Ref.: #66708]	Motility	no

[Ref.: #66708]	Colony size	3.1-4 mm
[Ref.: #66708]	Colony color	white to yellow
[Ref.: #66708]	Colony shape	circular
[Ref.: #66708]	Incubation period	3 days
[Ref.: #66708]	Cultivation medium used	PYF

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65104]

Culture medium

PY + X MEDIUM (DSMZ Medium 104b)

[Ref.: #65104]

Culture medium growth

[Ref.: #65104]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65104]

Culture medium composition

expand / minimize

[Ref.: #66708]

Culture medium

peptone-yeast-fructose (PYF) medium

[Ref.: #66708]

Culture medium growth

[Ref.: #66708]

Culture medium composition

expand / minimize

[Ref.: #66708]

Culture medium

PYF

[Ref.: #66708]

Culture medium growth

		Temperature
[Ref.: #65104]	growth	40 °C
[Ref.: #66708]	optimum	42 °C
[Ref.: #66708]	growth	30-50 °C

[Ref.: #65104]	Temperature range	thermophilic
[Ref.: #66708]	Temperature range	thermophilic
[Ref.: #66708]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66708]			optimum	5.5-6.0
[Ref.: #66708]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66708]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #66708]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.941
[Ref.: #69481]	Ability of spore formation	no Confidence in %93

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66708]	D-fructose ChEBI	+	assimilation
[Ref.: #66708]	D-fructose ChEBI	+	carbon source
[Ref.: #66708]	D-galactose ChEBI	-	assimilation
[Ref.: #66708]	D-gluconate ChEBI	+	carbon source
[Ref.: #66708]	D-glucose ChEBI	-	assimilation
[Ref.: #66708]	D-mannose ChEBI	-	assimilation
[Ref.: #66708]	D-ribose ChEBI	+	carbon source
[Ref.: #66708]	gluconate ChEBI	+	assimilation
[Ref.: #66708]	glycine ChEBI	+	carbon source
[Ref.: #66708]	L-alanine ChEBI	+	carbon source
[Ref.: #66708]	L-arabinose ChEBI	-	assimilation
[Ref.: #66708]	L-arginine ChEBI	+	carbon source
[Ref.: #66708]	L-isoleucine ChEBI	+	carbon source
[Ref.: #66708]	L-lysine ChEBI	+	carbon source
[Ref.: #66708]	L-phenylalanine ChEBI	+	carbon source
[Ref.: #66708]	L-proline ChEBI	+	carbon source
[Ref.: #66708]	L-serine ChEBI	+	carbon source
[Ref.: #66708]	L-tryptophan ChEBI	+	carbon source
[Ref.: #66708]	L-tyrosine ChEBI	+	carbon source
[Ref.: #66708]	lactate ChEBI	-	assimilation
[Ref.: #66708]	maltose ChEBI	-	assimilation
[Ref.: #66708]	mannitol ChEBI	-	assimilation
[Ref.: #66708]	pyruvate ChEBI	+	assimilation
[Ref.: #66708]	pyruvate ChEBI	+	carbon source
[Ref.: #66708]	succinate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #66708]	bacitracin ChEBI	yes	25	µg/mL
[Ref.: #66708]	chloramphenicol ChEBI	yes	25	µg/mL
[Ref.: #66708]	colistin ChEBI	yes	25	µg/mL
[Ref.: #66708]	erythromycin ChEBI	yes	25	µg/mL
[Ref.: #66708]	lincomycin ChEBI	yes	25	µg/mL
[Ref.: #66708]	penicillin ChEBI	yes	25	µg/mL
[Ref.: #66708]	streptomycin ChEBI				yes	27	µg/mL
[Ref.: #66708]	tetracycline ChEBI				yes	25	µg/mL
[Ref.: #66708]	vancomycin ChEBI				yes	28	µg/mL
[Ref.: #66708]	virginiamycin ChEBI				yes	26	µg/mL

	Metabolite	Production
[Ref.: #66708]	acetic acid ChEBI	yes
[Ref.: #66708]	formate ChEBI	yes
[Ref.: #66708]	phenylacetate ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66708]		catalase	-	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65104]	Sample type/isolated from	Healthy White Leghorn Chicken, cecum
[Ref.: #65104]	Geographic location (country and/or sea, region)	Iowa
[Ref.: #65104]	Country	USA
[Ref.: #65104]	Country ISO 3 Code	USA
[Ref.: #65104]	Continent	North America

[Ref.: #66708]	Sample type/isolated from	from the cecum of a healthy adult white leghorn chicken
[Ref.: #66708]	Enrichment culture	brain-heart infusion (BHI) medium (Difco)
[Ref.: #66708]	Enrichment culture composition	0.05 % (w/v) L-cysteine hydrochloride and 0.0001 % resazurin
[Ref.: #66708]	Enrichment culture duration	2 days
[Ref.: #66708]	Enrichment culture temperature	42 ̊C
[Ref.: #66708]	Isolation/enrichment procedure	brain-heart infusion (BHI) medium (Difco), supplemented with 0.05 % (w/v) L-cysteine hydrochloride and 0.0001 % resazurin. Cecal contents were diluted and plated before incubation for two days at 42C. C
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Gastrointestinal tract	#Large intestine
#Host	#Birds	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65104]	Megasphaera stantonii strain AJH120 16S ribosomal RNA gene, partial sequence	MG811574	1396	ENA	2144175 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Megasphaera stantonii AJH120	GCA_003367905	complete	GenBank	2144175 tax ID	*
[Ref.: #66792]	Megasphaera stantonii AJH120	GCA_003030445	scaffold	GenBank	2144175 tax ID	*
[Ref.: #66792]	Megasphaera stantonii AJH120	2841202678	complete	JGI IMG/M	2144175 tax ID	*

[Ref.: #65104]	GC-content	52.1 mol%
[Ref.: #66708]	GC-content	52.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Megasphaera stantonii AJH120 NCBI: GCA_003367905.1
[Ref.: #69481]	spore-forming	no	93	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Megasphaera stantonii AJH120 NCBI: GCA_003367905.1
[Ref.: #69480]	motile	no	91.628	yes
[Ref.: #69480]	flagellated	no	96.434	yes
[Ref.: #69480]	gram-positive	no	97.791	yes
[Ref.: #69480]	anaerobic	yes	99.629	no
[Ref.: #69480]	aerobic	no	98.354	yes
[Ref.: #69480]	halophile	no	79.041	no
[Ref.: #69480]	spore-forming	no	93.937	yes
[Ref.: #69480]	glucose-util	yes	67.665	yes
[Ref.: #69480]	thermophile	no	96.879	no
[Ref.: #69480]	glucose-ferment	yes	60.715	no

Availability in culture collections External links

[Ref.: #65104]

Culture collection no.

DSM 106750, CCUG 71842

[Ref.: #111229]

SI-ID 407607

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Megasphaera stantonii AJH120(T), Isolated from a Chicken Cecum.	Maki JJ, Looft T	Microbiol Resour Announc	10.1128/MRA.01148-18	2018	*
Phylogeny	Megasphaera stantonii sp. nov., a butyrate-producing bacterium isolated from the cecum of a healthy chicken.	Maki JJ, Looft T	Int J Syst Evol Microbiol	10.1099/ijsem.0.002991	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65104

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106750

#66708

Joel J. Maki, Torey Looft: Megasphaera stantonii sp. nov., a butyrate-producing bacterium isolated from the cecum of a healthy chicken. IJSEM 68: 3409 - 3415 2018 ( DOI 10.1099/ijsem.0.002991 , PubMed 30281016 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111229

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407607.1 )

* These data were automatically processed and therefore are not curated

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