225 ml University of Vermont Modification Medium Ibroth was added to the sediment (25±1 g) samples and incubated at 30°C for 24±2 h. Secondary enrichment was made by transferring 0.1 ml UVM I enrichment to 10ml UVM II broth. A loop full (10 µl) from the UVM II broth was then streaked onto polymyxin - acriflavin -lithium chloride - ceftazidime -aesculin - mannitol (PALCAM) agar plates and incubated at 35°C for 24 - 48 h. The inoculated UVM II was incubated at 30 C for 24±2 h
* These data were automatically processed and therefore are not curated
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