Name: Enteroscipio rubneri ResAG-96
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158401

Type strain:

Species: Enteroscipio rubneri

Strain Designation: ResAG-96

Culture col. no.: DSM 105130, JCM 32273

Strain history: M. Huch; Max Rubner-Inst., Germany; ResAG-96.

NCBI tax ID(s): 2070686 (species)

SI-ID 402505

DSM 105130

Section

Enteroscipio rubneri ResAG-96 is an obligate anaerobe, mesophilic, Gram-positive bacterium that was isolated from feacal sample, human.

Gram-positive
rod-shaped
mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Coriobacteriia*
	Order	*Eggerthellales*
	Family	*Eggerthellaceae*
	Genus	*Enteroscipio*
	Species	**Enteroscipio rubneri**
	Full Scientific Name (LPSN)	Enteroscipio rubneri Danylec et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65555]	Gram stain	positive

[Ref.: #65555]	Cell length	0.9 µm

[Ref.: #65555]	Cell width	0.4 µm

[Ref.: #65555]	Cell shape	rod-shaped

[Ref.: #65555]	Motility	no

[Ref.: #65555]	Colony color	pale-white
[Ref.: #65555]	Incubation period	2-3 days
[Ref.: #65555]	Cultivation medium used	BHI

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65077]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)

[Ref.: #65077]

Culture medium growth

[Ref.: #65077]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65077]

Culture medium composition

expand / minimize

[Ref.: #65555]

Culture medium

BHI

[Ref.: #65555]

Culture medium growth

		Temperature
[Ref.: #65077]	growth	37 °C
[Ref.: #65555]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #65077]	Temperature range	mesophilic
[Ref.: #65555]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65555]

Oxygen tolerance

obligate anaerobe

[Ref.: #67770]

Observation

quinones: MK-5, MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65555]	arabinose ChEBI	-	builds acid from
[Ref.: #65555]	cellobiose ChEBI	-	builds acid from
[Ref.: #65555]	esculin ChEBI	-	hydrolysis
[Ref.: #65555]	gelatin ChEBI	-	hydrolysis
[Ref.: #65555]	glucose ChEBI	-	builds acid from
[Ref.: #65555]	glycerol ChEBI	-	builds acid from
[Ref.: #65555]	lactose ChEBI	-	builds acid from
[Ref.: #65555]	maltose ChEBI	-	builds acid from
[Ref.: #65555]	mannitol ChEBI	-	builds acid from
[Ref.: #65555]	mannose ChEBI	-	builds acid from
[Ref.: #65555]	mannose ChEBI	-	fermentation
[Ref.: #65555]	melezitose ChEBI	-	builds acid from
[Ref.: #65555]	nitrate ChEBI	-	reduction
[Ref.: #65555]	raffinose ChEBI	-	builds acid from
[Ref.: #65555]	raffinose ChEBI	-	fermentation
[Ref.: #65555]	rhamnose ChEBI	-	builds acid from
[Ref.: #65555]	salicin ChEBI	-	builds acid from
[Ref.: #65555]	sorbitol ChEBI	-	builds acid from
[Ref.: #65555]	sucrose ChEBI	-	builds acid from
[Ref.: #65555]	trehalose ChEBI	-	builds acid from
[Ref.: #65555]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #65555]	ampicillin ChEBI	yes	1	µg/mL
[Ref.: #65555]	chloramphenicol ChEBI	yes	5	µg/mL
[Ref.: #65555]	colistin ChEBI	yes	5	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #65555]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65555]	alanine arylamidase	-	3.4.11.2
[Ref.: #65555]	alkaline phosphatase	-	3.1.3.1
[Ref.: #65555]	alpha-arabinosidase	-	3.2.1.55
[Ref.: #65555]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65555]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65555]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65555]	arginine arylamidase	-
[Ref.: #65555]	arginine dihydrolase	+	3.5.3.6
[Ref.: #65555]	beta-galactosidase	-	3.2.1.23
[Ref.: #65555]	beta-glucosidase	-	3.2.1.21
[Ref.: #65555]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65555]	catalase	-	1.11.1.6
[Ref.: #65555]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65555]	glutamate decarboxylase	-	4.1.1.15
[Ref.: #65555]	glutamyl-glutamate arylamidase	-
[Ref.: #65555]	glycin arylamidase	-
[Ref.: #65555]	histidine arylamidase	-
[Ref.: #65555]	leucine arylamidase	-	3.4.11.1
[Ref.: #65555]	leucyl glycin arylamidase	-	3.4.11.1
[Ref.: #65555]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65555]	phenylalanine arylamidase	-
[Ref.: #65555]	proline-arylamidase	-	3.4.11.5
[Ref.: #65555]	pyroglutamic acid arylamidase	-
[Ref.: #65555]	serine arylamidase	-
[Ref.: #65555]	tyrosine arylamidase	-
[Ref.: #65555]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65077]	Sample type/isolated from	feacal sample, human
[Ref.: #65077]	Geographic location (country and/or sea, region)	Karlsruhe
[Ref.: #65077]	Country	Germany
[Ref.: #65077]	Country ISO 3 Code	DEU
[Ref.: #65077]	Continent	Europe

[Ref.: #65555]	Sample type/isolated from	faecal sample of a 30 years old human, male, moderately obese (BMI 33.2 kg/m2)
[Ref.: #65555]	Geographic location (country and/or sea, region)	Karlsruhe
[Ref.: #65555]	Country	Germany
[Ref.: #65555]	Country ISO 3 Code	DEU
[Ref.: #65555]	Continent	Europe
[Ref.: #65555]	Enrichment culture	BHI
[Ref.: #65555]	Enrichment culture composition	10 ml BHI supplemented with ampicillin (1 µg/ml), colistin (5 µg/ml), chloramphenicol (5 µg/ml), cholic acid (18 µg/ml) and trans-resveratrol (80 µM)
[Ref.: #65555]	Enrichment culture duration	7 days
[Ref.: #65555]	Enrichment culture temperature	37 ̊C

[Ref.: #67770]	Sample type/isolated from	Fecal sample of a 30-year old, human, male, moderately obese volunteer
[Ref.: #67770]	Geographic location (country and/or sea, region)	Karlsruhe
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Male

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY595972 (>99% sequence identity) for Enteroscipio rubneri subclade from Microbeatlas

Aquatic Samples	251
Soil Samples	86
Animal Samples	14308
Plant Samples	24
Total Samples	14669

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65077]	Enteroscipio rubneri strain ResAG-96 16S ribosomal RNA gene, partial sequence	KY595972	1342	ENA	2070686 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Enteroscipio rubneri ResAG-96	GCA_002899715	contig	GenBank	2070686 tax ID
[Ref.: #66792]	Enteroscipio rubneri ResAG-96	2831391350	draft	JGI IMG/M	2070686 tax ID	*

[Ref.: #65077]	GC-content	62.4 mol%
[Ref.: #65555]	GC-content	62.4 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #65077]

Culture collection no.

DSM 105130, JCM 32273

[Ref.: #111205]

SI-ID 402505

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rubneribacter badeniensis gen. nov., sp. nov. and Enteroscipio rubneri gen. nov., sp. nov., new members of the Eggerthellaceae isolated from human faeces.	Danylec N, Gobl A, Stoll DA, Hetzer B, Kulling SE, Huch M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002705	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65077

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105130

#65555

Nicolas Danylec, Andrea Göbl, Dominic A. Stoll, Birgit Hetzer, Sabine E. Kulling and Melanie Huch: Rubneribacter badeniensis gen. nov., sp. nov. and Enteroscipio rubneri gen. nov., sp. nov., new members of the Eggerthellaceae isolated from human faeces. IJSEM 68: 1533 - 1540 2018 ( DOI 10.1099/ijsem.0.002705 , PubMed 29537365 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#111205

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402505.1 )

* These data were automatically processed and therefore are not curated

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