Name: Lactobacillus panisapium Bb203
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158390

Type strain:

Species: Lactobacillus panisapium

Strain Designation: Bb203

Culture col. no.: DSM 102188, ACCC 19955

Strain history: <- C. Wang, Biogas Inst. of Ministry of Agriculture, Chengdu, P.R. China; Bb203

NCBI tax ID(s): 2012495 (species)

SI-ID 404530

DSM 102188

Section

Lactobacillus panisapium Bb203 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from bee bread .

Gram-positive
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus panisapium**
	Full Scientific Name (LPSN)	Lactobacillus panisapium Wang et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65340]	Gram stain	positive

[Ref.: #65340]	Cell length	1.5-4.1 µm

[Ref.: #65340]	Cell width	0.3-0.45 µm

[Ref.: #65340]	Cell shape	rod-shaped

[Ref.: #65340]	Motility	no

[Ref.: #65340]	Colony size	2-3 mm
[Ref.: #65340]	Colony color	white
[Ref.: #65340]	Incubation period	2 days
[Ref.: #65340]	Cultivation medium used	Man Rogosa and Sharpe agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65066]

Culture medium

MRS MEDIUM WITH CYSTEINE (DSMZ Medium 232)

[Ref.: #65066]

Culture medium growth

[Ref.: #65066]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65066]

Culture medium composition

expand / minimize

[Ref.: #65340]

Culture medium

Man Rogosa and Sharpe agar

[Ref.: #65340]

Culture medium growth

		Temperature
[Ref.: #65066]	growth	37 °C
[Ref.: #65340]	growth	15-55 °C
[Ref.: #65340]	optimum	37 °C

[Ref.: #65066]	Temperature range	mesophilic
[Ref.: #65340]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65340]			optimum	6.5
[Ref.: #65340]			growth	3.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65340]

Oxygen tolerance

facultative anaerobe

[Ref.: #65340]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65340]		NaCl		growth	0-85	g/L
[Ref.: #65340]		NaCl		optimum	5	g/L

[Ref.: #65340]	Observation	Doubling time under the optimal conditions is 0.46 h.
[Ref.: #65340]	Observation	The main fermentation products from glucose are D-/L-lactic acid, acetic acid, propionic acid, butyric acid and CO 2 .

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65340]	cellobiose ChEBI	+	assimilation
[Ref.: #65340]	D-fructose ChEBI	+	assimilation
[Ref.: #65340]	D-galactose ChEBI	+	assimilation
[Ref.: #65340]	D-glucose ChEBI	+	assimilation
[Ref.: #65340]	D-mannose ChEBI	+	assimilation
[Ref.: #65340]	D-ribose ChEBI	+	assimilation
[Ref.: #65340]	esculin ChEBI	+	hydrolysis
[Ref.: #65340]	glucose ChEBI	+	fermentation
[Ref.: #65340]	L-arabinose ChEBI	+	assimilation
[Ref.: #65340]	L-rhamnose ChEBI	+	assimilation
[Ref.: #65340]	melibiose ChEBI	+	assimilation
[Ref.: #65340]	raffinose ChEBI	+	assimilation
[Ref.: #65340]	starch ChEBI	-	hydrolysis
[Ref.: #65340]	sucrose ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65340]		acetoin ChEBI	no
[Ref.: #65340]		hydrogen sulfide ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #65340]		acetoin ChEBI	-

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65340]		catalase	-	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65066]	Sample type/isolated from	bee bread (Apis cerana)
[Ref.: #65066]	Geographic location (country and/or sea, region)	Kunming, Yunnan Province
[Ref.: #65066]	Country	China
[Ref.: #65066]	Country ISO 3 Code	CHN
[Ref.: #65066]	Continent	Asia

[Ref.: #65340]	Sample type/isolated from	bee bread of Apis cerana collected from a hive
[Ref.: #65340]	Geographic location (country and/or sea, region)	Kunming, Yunnan province
[Ref.: #65340]	Country	China
[Ref.: #65340]	Country ISO 3 Code	CHN
[Ref.: #65340]	Continent	Asia
[Ref.: #65340]	Enrichment culture	Man Rogosa and Sharpe broth
[Ref.: #65340]	Enrichment culture composition	supplemented with 0.5 g l-1 L-cysteine, 1.0 mg l-1 resazurin and 15 g l-1 agar
[Ref.: #65340]	Enrichment culture duration	2 days
[Ref.: #65340]	Enrichment culture temperature	37 ̊C
[Ref.: #65340]	Isolation/enrichment procedure	bee bread was mashed and resuspended in sterilized 0.9 % NaCl under anaerobic conditions, followed by serial dilution in anaerobic culture tubes containing sterilized de Man Rogosa and Sharpe broth. The MRS medium was prepared and dispensed under a gaseous atmosphere of 100 % nitrogen. Strains were isolated by Hungate roll-tube technique. Incubation for 48 h at 37°C.
* marker position based on {}

Isolation sources categories

#Host

#Arthropoda

#Insecta

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65066]	Lactobacillus panisapium strain Bb 2-3 16S ribosomal RNA gene, partial sequence	KX447147	1437	ENA	2012495 tax ID

[Ref.: #65340]

GC-content

37.4 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #65066]

Culture collection no.

DSM 102188, ACCC 19955

[Ref.: #111194]

SI-ID 404530

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread.	Wang C, Huang Y, Li L, Guo J, Wu Z, Deng Y, Dai L, Ma S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002538	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65066

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102188

#65340

Cong Wang, Yan Huang, Li Li, Jun Guo, Zhengyun Wu, Yu Deng, Lirong Dai, Shichun Ma: Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread. IJSEM 68: 703 - 708 2018 ( DOI 10.1099/ijsem.0.002538 , PubMed 29458462 )

#111194

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404530.1 )

* These data were automatically processed and therefore are not curated

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