Tritonibacter horizontis (DSM 101689, LMG 29740, O3.65)

Name: Tritonibacter horizontis (DSM 101689, LMG 29740, O3.65)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158322

Type strain

Strain Designation O3.65

Culture col. no. DSM 101689 LMG 29740 O3.65

NCBI tax ID(s) 1768241

Special Collections DSMZ Literature strains

History <- H.-A. Giebel, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl-von-Ossietzky University Oldenburg; O3.65 <- K. Grosser and H.-A. Giebel, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl-von-Ossietzky University Oldenburg; O3.65

Links

Tritonibacter horizontis O3.65 is an aerobe, chemoorganoheterotroph, Gram-negative bacterium that forms circular colonies and was isolated from enrichment culture of surface seawater contaminated with weathered oil from the Deep Water Horizon oil spill.

Gram-negative motile rod-shaped colony-forming aerobe chemoorganoheterotroph genome sequence Bacteria

Name and taxonomic classification

SI-ID 408172

LMG 29740,DSM 101689

@ref 20215

Last LPSN update 2026-02-09 11:38:17

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodobacterales

Family Roseobacteraceae

Genus Tritonibacter

Species Tritonibacter horizontis

Full scientific name Tritonibacter horizontis Klotz et al. 2018

Synonyms (1)

Epibacterium horizontis

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement	Confidence
65345	negative	1.3-2.2 µm	0.6-1 µm	rod-shaped		monotrichous, polar
125439	negative						97.4

Colony morphology

@ref	Colony color	Colony shape	Medium used
65345	light beige	circular	marine broth

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
64998	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
65345	marine broth

Culture temp

@ref	Growth	Type	Temperature (°C)
64998	positive	growth	30
65345	positive	growth	4-45
65345	positive	optimum	30-35

Culture pH

@ref	Ability	Type	PH	PH range
65345	positive	optimum	7
65345	positive	growth	5.5-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65345	aerobe
125439	aerobe	92

Nutrition type

65345

Typechemoorganoheterotroph

Spore formation

@ref	Spore formation	Confidence
125439		95.9

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65345	NaCl	positive	growth	0.5-10 %
65345	NaCl	positive	optimum	3.5-4 %

Observation

65345

ObservationNo growth was observed on oil and paraffin containing agar plates.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65345	48095 ChEBI	(-)-D-fructose	+	carbon source
65345	32373 ChEBI	4-coumarate	+	assimilation
65345	17879 ChEBI	4-hydroxybenzoate	+	assimilation
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
65345	16449 ChEBI	alanine	+	assimilation
65345	29016 ChEBI	arginine	+	assimilation
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
65345	22653 ChEBI	asparagine	+	assimilation
65345	35391 ChEBI	aspartate	+	assimilation
65345	16150 ChEBI	benzoate	-	assimilation
65345	15956 ChEBI	biotin	+	required for growth
65345	17057 ChEBI	cellobiose	+	carbon source
65345	23248 ChEBI	cinnamate	-	assimilation
65345	16947 ChEBI	citrate	+	assimilation
65345	15356 ChEBI	cysteine	-	assimilation
65345	12936 ChEBI	D-galactose	+	carbon source
65345	17315 ChEBI	D-glucosamine	+	carbon source
65345	17634 ChEBI	D-glucose	+	carbon source
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
65345	16899 ChEBI	D-mannitol	+	carbon source
65345	16024 ChEBI	D-mannose	+	carbon source
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
65345	16988 ChEBI	D-ribose	+	carbon source
65345	65327 ChEBI	D-xylose	+	carbon source
65345	41808 ChEBI	decane	+	assimilation
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
65345	29749 ChEBI	ferulate	+	assimilation
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
65345	29987 ChEBI	glutamate	+	assimilation
65345	28300 ChEBI	glutamine	-	assimilation
65345	17754 ChEBI	glycerol	+	assimilation
65345	15428 ChEBI	glycine	-	assimilation
65345	45296 ChEBI	hexadecane	+	assimilation
65345	27570 ChEBI	histidine	+	assimilation
65345	24898 ChEBI	isoleucine	+	assimilation
65345	30849 ChEBI	L-arabinose	+	carbon source
65345	18287 ChEBI	L-fucose	+	carbon source
65345	17716 ChEBI	lactose	-	carbon source
65345	25017 ChEBI	leucine	+	assimilation
65345	25094 ChEBI	lysine	-	assimilation
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
65345	16811 ChEBI	methionine	-	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
65345	16301 ChEBI	nitrite	-	reduction
65345	32892 ChEBI	nonane	+	assimilation
65345	28044 ChEBI	phenylalanine	+	assimilation
65345	26271 ChEBI	proline	+	assimilation
65345	36241 ChEBI	protocatechuate	+	assimilation
65345	30762 ChEBI	salicylate	-	assimilation
65345	17822 ChEBI	serine	+	assimilation
65345	32954 ChEBI	sodium acetate	+	assimilation
65345	50144 ChEBI	sodium pyruvate	+	assimilation
65345		sodium(+)	+	required for growth
65345	28017 ChEBI	starch	-	carbon source
65345	17992 ChEBI	sucrose	+	carbon source
65345	26986 ChEBI	threonine	+	assimilation
65345	27897 ChEBI	tryptophan	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
65345	18186 ChEBI	tyrosine	+	assimilation
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
65345	27266 ChEBI	valine	+	assimilation
65345	16632 ChEBI	vanillate	+	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
65345	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68382	beta-glucosidase	-	3.2.1.21	from API zym
65345	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
65345	cytochrome oxidase	+	1.9.3.1
65345	gelatinase	-
68369	gelatinase	-		from API 20NE
68382	lipase (C 14)	-		from API zym
65345	lipase (Tween 80)	-
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	CMP-KDO biosynthesis	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	glycine betaine biosynthesis	100	5 of 5
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	adipate degradation	100	2 of 2
66794	allantoin degradation	100	9 of 9
66794	octane oxidation	100	3 of 3
66794	Entner Doudoroff pathway	100	10 of 10
66794	ethanol fermentation	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	folate polyglutamylation	100	1 of 1
66794	4-hydroxymandelate degradation	100	9 of 9
66794	methylglyoxal degradation	100	5 of 5
66794	suberin monomers biosynthesis	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	resorcinol degradation	100	2 of 2
66794	lactate fermentation	100	4 of 4
66794	gluconeogenesis	100	8 of 8
66794	NAD metabolism	94.44	17 of 18
66794	leucine metabolism	92.31	12 of 13
66794	phenylalanine metabolism	92.31	12 of 13
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	pyrimidine metabolism	86.67	39 of 45
66794	photosynthesis	85.71	12 of 14
66794	vitamin B1 metabolism	84.62	11 of 13
66794	purine metabolism	84.04	79 of 94
66794	vitamin B12 metabolism	82.35	28 of 34
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	tryptophan metabolism	81.58	31 of 38
66794	methionine metabolism	80.77	21 of 26
66794	3-chlorocatechol degradation	80	4 of 5
66794	gallate degradation	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	alanine metabolism	79.31	23 of 29
66794	tyrosine metabolism	78.57	11 of 14
66794	degradation of pentoses	78.57	22 of 28
66794	heme metabolism	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	histidine metabolism	75.86	22 of 29
66794	isoleucine metabolism	75	6 of 8
66794	carnitine metabolism	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	biotin biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	arginine metabolism	75	18 of 24
66794	flavin biosynthesis	73.33	11 of 15
66794	vitamin B6 metabolism	72.73	8 of 11
66794	pentose phosphate pathway	72.73	8 of 11
66794	proline metabolism	72.73	8 of 11
66794	d-xylose degradation	72.73	8 of 11
66794	degradation of sugar acids	72	18 of 25
66794	oxidative phosphorylation	71.43	65 of 91
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glutathione metabolism	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	glycolysis	70.59	12 of 17
66794	propionate fermentation	70	7 of 10
66794	phenol degradation	70	14 of 20
66794	4-hydroxyphenylacetate degradation	70	7 of 10
66794	lysine metabolism	69.05	29 of 42
66794	degradation of sugar alcohols	68.75	11 of 16
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	IAA biosynthesis	66.67	2 of 3
66794	degradation of aromatic, nitrogen containing compounds	66.67	8 of 12
66794	cyanate degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	non-pathway related	65.79	25 of 38
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	urea cycle	61.54	8 of 13
66794	lipid metabolism	61.29	19 of 31
66794	hydrogen production	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	glycogen metabolism	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	vitamin K metabolism	60	3 of 5
66794	3-phenylpropionate degradation	60	9 of 15
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	propanol degradation	57.14	4 of 7
66794	degradation of hexoses	55.56	10 of 18
66794	cysteine metabolism	55.56	10 of 18
66794	phosphatidylethanolamine bioynthesis	53.85	7 of 13
66794	sulfate reduction	53.85	7 of 13
66794	selenocysteine biosynthesis	50	3 of 6
66794	kanosamine biosynthesis II	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	sulfopterin metabolism	50	2 of 4
66794	pantothenate biosynthesis	50	3 of 6
66794	phenylmercury acetate degradation	50	1 of 2
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	ascorbate metabolism	50	11 of 22
66794	ribulose monophosphate pathway	50	1 of 2
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	cyclohexanol degradation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	ketogluconate metabolism	50	4 of 8
66794	toluene degradation	50	2 of 4
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	metabolism of disaccharids	45.45	5 of 11
66794	nitrate assimilation	44.44	4 of 9
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	cellulose degradation	40	2 of 5
66794	factor 420 biosynthesis	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	polyamine pathway	39.13	9 of 23
66794	arachidonic acid metabolism	38.89	7 of 18
66794	sphingosine metabolism	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	ginsenoside metabolism	31.25	5 of 16
66794	starch degradation	30	3 of 10
66794	coenzyme M biosynthesis	30	3 of 10
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	methanogenesis from CO2	25	3 of 12
66794	vitamin E metabolism	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	alginate biosynthesis	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	daunorubicin biosynthesis	22.22	2 of 9

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
64998	-	+	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
64998	-	+	+/-	+/-	-	+	-	-	-	-	-	+/-	+/-	-	+	-	-	-	-	-
64998	-	+	+/-	-	-	+	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
64998	-	-	-	-	-	+	-	+	+/-	+/-	+	-	+	+	+	-	+	+	+	-	not determinedn.d.
64998	-	-	-	-	-	+	-	+	+/-	+/-	+	-	+	+	+	-	+	+	+	-	not determinedn.d.
64998	-	-	-	-	-	+	-	+	+	+	+	+/-	+	+	+	-	+	+	+	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Laboratory	#Lab enrichment
#Environmental	#Aquatic	#Marine
#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Aquatic	#Surface water

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Sampling date
64998	enrichment culture of surface seawater contaminated with weathered oil (slicks) from the Deep Water Horizon (DWH) oil spill	Gulf of Mexiko, (28° 43.967 N, 88° 22.993 W)	USA	USA	North America	28.7328	-88.3832 28.7328/-88.3832
65345	oil-contaminated surface seawater	Macondo wellhead, Golf of Mexico				28.7327	-88.3832 28.7327/-88.3832	2010-06-01

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM151801v1 assembly for Tritonibacter horizontis O3.65	contig	GCA_001518015	1768241.5	2788500136	1768241	47.34

GC content

@ref	GC-content (mol%)	Method
64998	61.5
65345	61.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Tritonibacter horizontis O3.65
NCBI: GCA_001518015

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	95.90	no
125439	motility	BacteriaNetⓘ	yes	68.60	no
125439	gram_stain	BacteriaNetⓘ	negative	97.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	92.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tritonibacter horizontis gen. nov., sp. nov., a member of the Rhodobacteraceae, isolated from the Deepwater Horizon oil spill.	Klotz F, Brinkhoff T, Freese HM, Wietz M, Teske A, Simon M, Giebel HA	Int J Syst Evol Microbiol	10.1099/ijsem.0.002573	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64998	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101689
#65345	Franziska Klotz, Thorsten Brinkhoff, Heike M. Freese, Matthias Wietz, Andreas Teske, Meinhard Simon, Helge-Ansgar Giebel: Tritonibacter horizontis gen. nov., sp. nov., a member of the Rhodobacteraceae, isolated from the Deepwater Horizon oil spill. IJSEM 68: 736 - 744 2018 ( DOI 10.1099/ijsem.0.002573 , PubMed 29458459 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Tritonibacter horizontis (DSM 101689, LMG 29740, O3.65)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_001518015

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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