Nocardioides houyundeii 78 | Type strain | DSM 106424, CGMCC 4.7461

Caption:

Medium 215 28°C

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

BHI MEDIUM (DSMZ Medium 215)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

BHI-5% sheep blood agar

Culture medium growth

Culture medium

BHI agar

Culture medium growth

Culture medium

Reasoner's 2A agar (R2A)

Culture medium growth

Culture medium

tryptic soy agar (TSA)

Culture medium growth

		Temperature
[Ref.: #64990]	growth	28 °C
[Ref.: #66779]	optimum	28 °C
[Ref.: #66779]	growth	14-32 °C

[Ref.: #64990]	Temperature range	mesophilic
[Ref.: #66779]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66779]			optimum	7
[Ref.: #66779]			growth	6.5-8.0

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.958
[Ref.: #69481]	Ability of spore formation	no Confidence in %92

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66779]		NaCl		growth	0.5-1.5	%(w/v)

What are isolation sources categories?

Observation

The predominant menaquinone is MK-8 (H4) and the polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66779]	amygdalin ChEBI	-	builds acid from
[Ref.: #66779]	arabinitol ChEBI	-	builds acid from
[Ref.: #66779]	arbutin ChEBI	-	builds acid from
[Ref.: #66779]	cellobiose ChEBI	-	builds acid from
[Ref.: #66779]	D-arabinose ChEBI	-	builds acid from
[Ref.: #66779]	D-fructose ChEBI	-	builds acid from
[Ref.: #66779]	D-fucose ChEBI	-	builds acid from
[Ref.: #66779]	D-galactose ChEBI	-	builds acid from
[Ref.: #66779]	D-glucose ChEBI	-	builds acid from
[Ref.: #66779]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66779]	D-mannose ChEBI	-	builds acid from
[Ref.: #66779]	D-ribose ChEBI	+	builds acid from
[Ref.: #66779]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66779]	D-xylose ChEBI	-	builds acid from
[Ref.: #66779]	erythritol ChEBI	-	builds acid from
[Ref.: #66779]	esculin ferric citrate	+	builds acid from
[Ref.: #66779]	galactitol ChEBI	-	builds acid from
[Ref.: #66779]	glycerol ChEBI	-	builds acid from
[Ref.: #66779]	glycogen ChEBI	-	builds acid from
[Ref.: #66779]	inulin ChEBI	-	builds acid from
[Ref.: #66779]	L-arabinose ChEBI	-	builds acid from
[Ref.: #66779]	L-fucose ChEBI	-	builds acid from
[Ref.: #66779]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #66779]	L-sorbose ChEBI	-	builds acid from
[Ref.: #66779]	lactose ChEBI	-	builds acid from
[Ref.: #66779]	maltose ChEBI	-	builds acid from
[Ref.: #66779]	melezitose ChEBI	-	builds acid from
[Ref.: #66779]	melibiose ChEBI	-	builds acid from
[Ref.: #66779]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #66779]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #66779]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66779]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #66779]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #66779]	raffinose ChEBI	-	builds acid from
[Ref.: #66779]	ribitol ChEBI	-	builds acid from
[Ref.: #66779]	salicin ChEBI	-	builds acid from
[Ref.: #66779]	sucrose ChEBI	-	builds acid from
[Ref.: #66779]	trehalose ChEBI	-	builds acid from
[Ref.: #66779]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66779]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #66779]	acid phosphatase	-	3.1.3.2
[Ref.: #66779]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66779]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66779]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66779]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66779]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66779]	beta-galactosidase	-	3.2.1.23
[Ref.: #66779]	beta-glucosidase	-	3.2.1.21
[Ref.: #66779]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66779]	beta-mannosidase	-	3.2.1.25
[Ref.: #66779]	catalase	+	1.11.1.6
[Ref.: #66779]	cystine arylamidase	-	3.4.11.3
[Ref.: #66779]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66779]	esterase (C 4)	+
[Ref.: #66779]	esterase Lipase (C 8)	+
[Ref.: #66779]	gelatinase	-
[Ref.: #66779]	leucine arylamidase	+	3.4.11.1
[Ref.: #66779]	lipase (C 14)	-
[Ref.: #66779]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66779]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66779]	nitrate reductase	-	1.7.99.4
[Ref.: #66779]	trypsin	-	3.4.21.4
[Ref.: #66779]	urease	-	3.5.1.5
[Ref.: #66779]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64990]	Sample type/isolated from	feces of the Tibetan antelope
[Ref.: #64990]	Geographic location (country and/or sea, region)	qinghai province, haixi city, Kekexili (35° 21' 55.7'' N, 93° 26' 38'' E)
[Ref.: #64990]	Country	China
[Ref.: #64990]	Country ISO 3 Code	CHN
[Ref.: #64990]	Continent	Asia
[Ref.: #64990]	Geographic location	35.3655°/93.4439°

[Ref.: #66779]	Sample type/isolated from	faecal sample of Tibetan antelope
[Ref.: #66779]	Sampling date	2014
[Ref.: #66779]	Geographic location (country and/or sea, region)	Hoh Xil Nature Reserve, Qinghai-Tibet Plateau
[Ref.: #66779]	Country	China
[Ref.: #66779]	Country ISO 3 Code	CHN
[Ref.: #66779]	Continent	Asia
[Ref.: #66779]	Geographic location	35.3655°/93.4439°
[Ref.: #66779]	Enrichment culture	brain-heart infusion (BHI) agar
[Ref.: #66779]	Enrichment culture composition	supplemented with 5% sheep blood (per litre: 12.5 g brain infusion solids, 5 g beefheart infusion solids, 10 g proteose peptone, 5 g NaCl, 2 g glucose, 2.5 g disodium phosphate, 10 g agar and 50 ml sterile defibrinated sheep blood; pH 7.4)
[Ref.: #66779]	Enrichment culture duration	7 days
[Ref.: #66779]	Enrichment culture temperature	28 ̊C
[Ref.: #66779]	Isolation/enrichment procedure	The faecal samples were processed as follows: the sample (1 g) was suspended in 200 µl of sterile water, 100 µl of the suspension was spread on brain-heart infusion (BHI) agar
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

[Ref.: #69479]

Global distribution of 16S sequence MG209819 (>99% sequence identity) for Nocardioides houyundeii subclade from Microbeatlas

Aquatic Samples	162
Soil Samples	2082
Animal Samples	286
Plant Samples	170
Total Samples	2700

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66779]	Nocardioides sp. strain 78 16S ribosomal RNA gene, partial sequence	MG209819	1480	ENA	35761 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Nocardioides houyundeii 78	GCA_002865585	complete	GenBank	2045452 tax ID	*
[Ref.: #66792]	Nocardioides houyundeii 78	2775506984	complete	JGI IMG/M	2045452 tax ID	*

GC-content

71.2 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nocardioides houyundeii 78 NCBI: GCA_002865585.1
[Ref.: #69481]	spore-forming	no	92	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nocardioides houyundeii 78 NCBI: GCA_002865585.1
[Ref.: #69480]	flagellated	no	93.839	yes
[Ref.: #69480]	gram-positive	yes	89.528	yes
[Ref.: #69480]	anaerobic	no	98.701	yes
[Ref.: #69480]	aerobic	yes	93.424	yes
[Ref.: #69480]	halophile	no	94.81	no
[Ref.: #69480]	spore-forming	no	79.23	no
[Ref.: #69480]	glucose-util	yes	86.232	no
[Ref.: #69480]	thermophile	no	99.106	yes
[Ref.: #69480]	motile	no	76.864	yes
[Ref.: #69480]	glucose-ferment	no	89.405	no

Availability in culture collections External links