Lentzea pudingi (DSM 108672, CGMCC 4.7319, KCTC 39694)

Name: Lentzea pudingi (DSM 108672, CGMCC 4.7319, KCTC 39694)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158291

Type strain

Culture col. no. DSM 108672 CGMCC 4.7319 KCTC 39694 DHS C021

NCBI tax ID(s) 1789439

Special Collections DSMZ KCTC Literature strains

History DSMZ <- J.-S. Lee, KCTC; KCTC 39694 <- C. Cao, Jiangsu Normal Univ., China; DHS C021 KCTC <- chengliang Cao, Jiangsu Normal Univ.

Links

Lentzea pudingi DSM 108672 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from From a weathered limestone sample in a karst area.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 404036

KCTC 39694,DSM 108672

@ref 20215

Last LPSN update 2026-05-29 08:43:56

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Lentzea

Species Lentzea pudingi

Full scientific name Lentzea pudingi Cao et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Confidence
65501	positive
125439		92.37
125439	positive	97.932

Colony morphology

65501

Medium usedISP 2

Multicellular morphology

@ref	Forms multicellular complex	Complex name	Complex color
65501		substrate mycelium	slightly yellow
65501		aerial mycelium	light grey

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
64951	ISP2 MEDIUM (DSMZ Medium 987)	Medium recipe at MediaDive	Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
65501	ISP 3
65501	ISP 4
65501	ISP 5
65501	Czapek's agar
65501	Nutrient agar (NA)
65501	ISP 2
65501	PDA

Culture temp

@ref	Growth	Type	Temperature (°C)
64951	positive	growth	28
65501	positive	growth	15-37
65501	positive	optimum	28
67771	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
65501	positive	optimum	7-8
65501	positive	growth	6-10	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65501	aerobe
67771	aerobe
125438	aerobe	92.029
125439	obligate aerobe	96.608

Spore formation

@ref	Spore description	Spore formation
65501	mature aerial mycelium develops into rod-shaped spore chains

Halophily

@ref	Halophily level	Salt	Growth	Tested relation	Concentration
65501		NaCl	positive	growth	5 %(w/v)
65501	non-halophilic	NaCl	positive	optimum	0 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
65501		casein	+	hydrolysis
65501	17108 ChEBI	D-arabinose	+	carbon source
65501	12936 ChEBI	D-galactose	-	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
65501	16024 ChEBI	D-mannose	-	carbon source
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
65501	63150 ChEBI	D-rhamnose	+	carbon source
65501	16988 ChEBI	D-ribose	-	carbon source
65501	17924 ChEBI	D-sorbitol	-	carbon source
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
65501	29985 ChEBI	L-glutamate	+	nitrogen source
65501	15971 ChEBI	L-histidine	+	nitrogen source
65501	18019 ChEBI	L-lysine	-	nitrogen source
65501	16414 ChEBI	L-valine	+	nitrogen source
65501	17716 ChEBI	lactose	+	carbon source
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
65501	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
65501	16634 ChEBI	raffinose	+	carbon source
65501	28017 ChEBI	starch	+	hydrolysis
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
65501	53426 ChEBI	tween 80	+	hydrolysis
65501	16199 ChEBI	urea	+/-	degradation
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
65501	17151 ChEBI	xylitol	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
65501	16136 ChEBI	hydrogen sulfide
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
65501	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	+		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
64951	-	+	+/-	-	-	+	+/-	-	-	+/-	-	+/-	-	+	-	+	-	+	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
64951	+	-	-	-	-	+	+	+	+	+	+	+	+	+	+	-	+	+	+/-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Geologic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature
67771	From a weathered limestone sample in a karst area		China	CHN	Asia
64951	a weathered limestone sample in a karst area	Guizhou Province, Puding Karst Ecosystem Research Station (26° 09'–26° 31' N, 105° 27'–105° 58' E)	China	CHN	Asia	26.15	105.45 26.15/105.45
65501	limestone sample	Puding Karst Ecosystem Research Station, Guizhou Province	China	CHN	Asia	26.15	105.45 26.15/105.45	culture medium consisting (per litre): 1.0 g sodium humate, 0.5 g Na2HPO4 , 1.5 g KCl, 0.05 g MgSO4 . 7H2O, 0.01 g FeSO4 . 7H2O and 0.02 g CaCO3. The pH was adjusted at 7.2 ± 0.1. After sterilizing, following antibiotics were added (per litre): 20 mg nalidixic acid, 40 mg cycloheximide and 40 mg nystatin.	21 days	28

Taxonmaps

69479

Global distribution of 16S sequence KU564532 (>99% sequence identity) for Lentzea from Microbeatlas

Aquatic Samples	22
Soil Samples	287
Animal Samples	13
Plant Samples	45
Total Samples	367

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64951	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1464625v1 assembly for Lentzea pudingi CGMCC 4.7319	scaffold	GCA_014646255	1789439.3	2989378172	1789439	66.26

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64951	Lentzea pudingi strain DHS C021 16S ribosomal RNA gene, partial sequence	KU564532	1466		1789439

GC content

@ref	GC-content (mol%)	Method
65501	69.8	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1464625v1 assembly for Lentzea pudingi CGMCC 4.7319
BV-BRC: 1789439.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.61	no
125439	gram_stain	BacteriaNetⓘ	positive	97.93	no
125439	motility	BacteriaNetⓘ	no	92.37	no
125439	spore_formation	BacteriaNetⓘ	yes	57.56	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lentzea pudingi CGMCC 4.7319
NCBI: GCA_014646255.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	88.72	no
125438	anaerobic	anaerobicⓘ	no	98.43	yes
125438	aerobic	aerobicⓘ	yes	92.03	yes
125438	spore-forming	spore-formingⓘ	yes	88.92	yes
125438	thermophilic	thermophileⓘ	no	97.00	no
125438	flagellated	motile2+ⓘ	no	88.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	1-Acetyl-beta-Carboline from a Jeju Gotjawal Strain Lentzea sp. JNUCC 0626 and Its Melanogenic Stimulating Activity in B16F10 Melanoma Cells.	Hyun KA, Xu Y, Boo KH, Hyun CG.	Molecules	10.3390/molecules29194586	2024
Phylogeny	Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area.	Cao C, Yuan B, Qin S, Jiang J, Tao F, Lian B	Int J Syst Evol Microbiol	10.1099/ijsem.0.002400	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64951	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108672
#65501	Chengliang Cao, Bo Yuan, Sheng Qin, Jihong Jiang, Faxiang Tao and Bin Lian: Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area. IJSEM 67: 4873 - 4878 2017 ( DOI 10.1099/ijsem.0.002400 , PubMed 29022551 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Lentzea pudingi (DSM 108672, CGMCC 4.7319, KCTC 39694)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014646255

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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