Name: Lentzea pudingi DSM 108672
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158291

Type strain:

Species: Lentzea pudingi

Culture col. no.: DSM 108672, CGMCC 4.7319, KCTC 39694, DHS C021

Strain history: <- chengliang Cao, Jiangsu Normal Univ.

NCBI tax ID(s): 1789439 (species)

SI-ID 404036

DSM 108672

Section

Lentzea pudingi DSM 108672 is an aerobe, spore-forming, mesophilic bacterium that builds a substrate mycelium and was isolated from From a weathered limestone sample in a karst area.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Lentzea*
	Species	**Lentzea pudingi**
	Full Scientific Name (LPSN)	Lentzea pudingi Cao et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65501]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #65501]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.843

[Ref.: #65501]

Cultivation medium used

ISP 2

[Ref.: #65501]	Multicellular complex forming ability	yes
[Ref.: #65501]	Multicellular complex name	substrate mycelium
[Ref.: #65501]	Multicellular complex color	slightly yellow

[Ref.: #65501]	Multicellular complex forming ability	yes
[Ref.: #65501]	Multicellular complex name	aerial mycelium
[Ref.: #65501]	Multicellular complex color	light grey

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64951]

Culture medium

ISP2 MEDIUM (DSMZ Medium 987)

[Ref.: #64951]

Culture medium growth

[Ref.: #64951]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64951]

Culture medium composition

expand / minimize

[Ref.: #65501]

Culture medium

ISP 3

[Ref.: #65501]

Culture medium growth

[Ref.: #65501]

Culture medium

ISP 4

[Ref.: #65501]

Culture medium growth

[Ref.: #65501]

Culture medium

ISP 5

[Ref.: #65501]

Culture medium growth

[Ref.: #65501]

Culture medium

Czapek's agar

[Ref.: #65501]

Culture medium growth

[Ref.: #65501]

Culture medium

Nutrient agar (NA)

[Ref.: #65501]

Culture medium growth

[Ref.: #65501]

Culture medium

ISP 2

[Ref.: #65501]

Culture medium growth

[Ref.: #65501]

Culture medium

PDA

[Ref.: #65501]

Culture medium growth

		Temperature
[Ref.: #64951]	growth	28 °C
[Ref.: #65501]	growth	15-37 °C
[Ref.: #65501]	optimum	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #64951]	Temperature range	mesophilic
[Ref.: #65501]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65501]			optimum	7-8
[Ref.: #65501]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65501]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #65501]	Spore description	mature aerial mycelium develops into rod-shaped spore chains
[Ref.: #65501]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100
[Ref.: #69481]	Ability of spore formation	yes Confidence in %92

[Ref.: #65501]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65501]		NaCl		growth	5	%(w/v)
[Ref.: #65501]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #65501]	casein	+	hydrolysis
[Ref.: #65501]	D-arabinose ChEBI	+	carbon source
[Ref.: #65501]	D-galactose ChEBI	-	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #65501]	D-mannose ChEBI	-	carbon source
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #65501]	D-rhamnose ChEBI	+	carbon source
[Ref.: #65501]	D-ribose ChEBI	-	carbon source
[Ref.: #65501]	D-sorbitol ChEBI	-	carbon source
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #65501]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #65501]	L-histidine ChEBI	+	nitrogen source
[Ref.: #65501]	L-lysine ChEBI	-	nitrogen source
[Ref.: #65501]	L-valine ChEBI	+	nitrogen source
[Ref.: #65501]	lactose ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #65501]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #65501]	raffinose ChEBI	+	carbon source
[Ref.: #65501]	starch ChEBI	+	hydrolysis
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #65501]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65501]	urea ChEBI	+/-	degradation
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #65501]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65501]		hydrogen sulfide ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #65501]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #64951]	24804	+	-	-	-	-	+	+	+	+	+	+	+	+	+	+	-	+	+	+/-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #64951]	14139	-	+	+/-	-	-	+	+/-	-	-	+/-	-	+/-	-	+	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67771]	Sample type/isolated from	From a weathered limestone sample in a karst area
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia

[Ref.: #64951]	Sample type/isolated from	a weathered limestone sample in a karst area
[Ref.: #64951]	Geographic location (country and/or sea, region)	Guizhou Province, Puding Karst Ecosystem Research Station (26° 09'–26° 31' N, 105° 27'–105° 58' E)
[Ref.: #64951]	Country	China
[Ref.: #64951]	Country ISO 3 Code	CHN
[Ref.: #64951]	Continent	Asia
[Ref.: #64951]	Geographic location	26.1500°/105.4500°

[Ref.: #65501]	Sample type/isolated from	limestone sample
[Ref.: #65501]	Geographic location (country and/or sea, region)	Puding Karst Ecosystem Research Station, Guizhou Province
[Ref.: #65501]	Country	China
[Ref.: #65501]	Country ISO 3 Code	CHN
[Ref.: #65501]	Continent	Asia
[Ref.: #65501]	Geographic location	26.1500°/105.4500°
[Ref.: #65501]	Enrichment culture composition	culture medium consisting (per litre): 1.0 g sodium humate, 0.5 g Na2HPO4 , 1.5 g KCl, 0.05 g MgSO4 . 7H2O, 0.01 g FeSO4 . 7H2O and 0.02 g CaCO3. The pH was adjusted at 7.2 ± 0.1. After sterilizing, following antibiotics were added (per litre): 20 mg nalidixic acid, 40 mg cycloheximide and 40 mg nystatin.
[Ref.: #65501]	Enrichment culture duration	21 days
[Ref.: #65501]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU564532 (>99% sequence identity) for Lentzea from Microbeatlas

Aquatic Samples	22
Soil Samples	287
Animal Samples	13
Plant Samples	45
Total Samples	367

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64951]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64951]	Lentzea pudingi strain DHS C021 16S ribosomal RNA gene, partial sequence	KU564532	1466	ENA	1789439 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lentzea pudingi CGMCC 4.7319	GCA_014646255	scaffold	GenBank	1789439 tax ID	*
[Ref.: #66792]	Lentzea pudingi strain CGMCC 4.7319	1789439.3	wgs	PATRIC	1789439 tax ID	*

[Ref.: #65501]

GC-content

69.8 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lentzea pudingi CGMCC 4.7319 NCBI: GCA_014646255.1
[Ref.: #69481]	spore-forming	yes	92	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lentzea pudingi CGMCC 4.7319 NCBI: GCA_014646255.1
[Ref.: #69480]	flagellated	no	96.805	yes
[Ref.: #69480]	gram-positive	yes	89.359	yes
[Ref.: #69480]	anaerobic	no	99.002	yes
[Ref.: #69480]	aerobic	yes	92.853	yes
[Ref.: #69480]	halophile	no	84.996	no
[Ref.: #69480]	spore-forming	yes	94.716	no
[Ref.: #69480]	thermophile	no	99.011	yes
[Ref.: #69480]	glucose-util	yes	89.382	yes
[Ref.: #69480]	motile	no	91.123	yes
[Ref.: #69480]	glucose-ferment	no	91.597	no

Availability in culture collections External links

[Ref.: #64951]

Culture collection no.

DSM 108672, CGMCC 4.7319, KCTC 39694, DHS C021

[Ref.: #111108]

SI-ID 404036

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area.	Cao C, Yuan B, Qin S, Jiang J, Tao F, Lian B	Int J Syst Evol Microbiol	10.1099/ijsem.0.002400	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64951

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108672

#65501

Chengliang Cao, Bo Yuan, Sheng Qin, Jihong Jiang, Faxiang Tao and Bin Lian: Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area. IJSEM 67: 4873 - 4878 2017 ( DOI 10.1099/ijsem.0.002400 , PubMed 29022551 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111108

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404036.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Lentzea pudingi in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Lentzea pudingi CGMCC 4.7319

Lentzea pudingi CGMCC 4.7319

Change proposal

Change proposal