Name: Lentzea rhizosphaerae NEAU-A2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158285

Type strain:

Species: Lentzea rhizosphaerae

Strain Designation: NEAU-A2

Culture col. no.: DSM 104541, CGMCC 4.7405

Strain history: <- J. Zhao, School of Life Science, Northeast Agricultural Univ. (NEAU), Harbin, China; NEAU-A2 <- W. Li, School of Life Science, NEAU, Harbin, China

NCBI tax ID(s): 2041025 (species)

SI-ID 405219

DSM 104541

Section

Lentzea rhizosphaerae NEAU-A2 is an aerobe, mesophilic, Gram-positive bacterium that builds aerial nycelium and was isolated from rhizosphere soil of wheat L..

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Lentzea*
	Species	**Lentzea rhizosphaerae**
	Full Scientific Name (LPSN)	Lentzea rhizosphaerae (Zhao et al. 2017) Yang and Zhi 2020

Synonym

Lechevalieria rhizosphaerae

Information on morphological and physiological properties Morphology

[Ref.: #65500]	Gram stain	positive

[Ref.: #65500]	Cell shape	rod-shaped

[Ref.: #65500]	Colony color	white
[Ref.: #65500]	Cultivation medium used	ISP 2

[Ref.: #65500]	Colony color	white
[Ref.: #65500]	Cultivation medium used	ISP 3

[Ref.: #65500]	Colony color	yellowish-white
[Ref.: #65500]	Cultivation medium used	ISP 4

[Ref.: #65500]	Colony color	white
[Ref.: #65500]	Cultivation medium used	ISP 6

[Ref.: #65500]	Colony color	white
[Ref.: #65500]	Cultivation medium used	ISP 7

[Ref.: #65500]	Colony color	white
[Ref.: #65500]	Cultivation medium used	Czapek_s agar

[Ref.: #65500]	Colony color	white
[Ref.: #65500]	Cultivation medium used	Bennett's agar

[Ref.: #65500]	Colony color	yellowish-white
[Ref.: #65500]	Cultivation medium used	nutrient agar

[Ref.: #65500]	Multicellular complex forming ability	yes
[Ref.: #65500]	Multicellular complex name	aerial nycelium

[Ref.: #65500]	Multicellular complex forming ability	yes
[Ref.: #65500]	Multicellular complex name	substrate hyphae

Multimedia content

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Caption:

Medium 65 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Czapek's agar

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Culture medium growth

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Culture medium

ISP 2

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Culture medium

ISP 3

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Culture medium growth

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Culture medium

ISP 4

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Culture medium

ISP 6

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Culture medium growth

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Culture medium

ISP 7

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Culture medium growth

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Culture medium

Czapek_s agar

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Culture medium growth

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Culture medium

Bennett's agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

		Temperature
[Ref.: #64945]	growth	28 °C
[Ref.: #65500]	growth	15-40 °C
[Ref.: #65500]	optimum	28 °C

[Ref.: #64945]	Temperature range	mesophilic
[Ref.: #65500]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65500]			optimum	7.0
[Ref.: #65500]			growth	5.0-11.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65500]		NaCl		growth	0-3	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65500]	D-fructose ChEBI	+	carbon source
[Ref.: #65500]	D-galactose ChEBI	+	carbon source
[Ref.: #65500]	D-glucose ChEBI	+	carbon source
[Ref.: #65500]	D-mannitol ChEBI	+	carbon source
[Ref.: #65500]	D-ribose ChEBI	-	carbon source
[Ref.: #65500]	D-sorbitol ChEBI	+	carbon source
[Ref.: #65500]	D-xylose ChEBI	-	carbon source
[Ref.: #65500]	esculin ChEBI	-	hydrolysis
[Ref.: #65500]	gelatin ChEBI	-
[Ref.: #65500]	glycine ChEBI	+	nitrogen source
[Ref.: #65500]	L-alanine ChEBI	+	nitrogen source
[Ref.: #65500]	L-arabinose ChEBI	+	carbon source
[Ref.: #65500]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #65500]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #65500]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #65500]	L-proline ChEBI	+	nitrogen source
[Ref.: #65500]	L-rhamnose ChEBI	+	carbon source
[Ref.: #65500]	L-serine ChEBI	+	nitrogen source
[Ref.: #65500]	L-threonine ChEBI	+	nitrogen source
[Ref.: #65500]	L-tyrosine ChEBI	+	nitrogen source
[Ref.: #65500]	lactose ChEBI	+	carbon source
[Ref.: #65500]	maltose ChEBI	+	carbon source
[Ref.: #65500]	myo-inositol ChEBI	+	carbon source
[Ref.: #65500]	nitrate ChEBI	-	reduction
[Ref.: #65500]	raffinose ChEBI	+	carbon source
[Ref.: #65500]	starch ChEBI	+	hydrolysis
[Ref.: #65500]	sucrose ChEBI	+	carbon source
[Ref.: #65500]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65500]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65500]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65500]		hydrogen sulfide ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65500]		cellulase	+	3.2.1.4
[Ref.: #65500]		urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C16:0	37.4
C15:0 anteiso	11.4
C16:1ω7c	10.8
C17:0 anteiso	10.4
C15:0 iso	9.7
C18:0	6.8
C17:0 10-methyl	5.7
C17:1ω7c	4.4
C16:0	1.8
C14:0 iso	1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64945]	Sample type/isolated from	rhizosphere soil of wheat (Triticum aestivum) L.
[Ref.: #64945]	Geographic location (country and/or sea, region)	Hebei province, Langfang (39° 32' N, 116° 40' E)
[Ref.: #64945]	Country	China
[Ref.: #64945]	Country ISO 3 Code	CHN
[Ref.: #64945]	Continent	Asia
[Ref.: #64945]	Geographic location	39.5333°/116.6670°

[Ref.: #65500]	Sample type/isolated from	rhizosphere soil of wheat (Triticum aestivum)
[Ref.: #65500]	Geographic location (country and/or sea, region)	Langfang, Hebei Province
[Ref.: #65500]	Country	China
[Ref.: #65500]	Country ISO 3 Code	CHN
[Ref.: #65500]	Continent	Asia
[Ref.: #65500]	Geographic location	39.5333°/116.6670°
[Ref.: #65500]	Enrichment culture	humic acid-vitamin agar (HV)
[Ref.: #65500]	Enrichment culture composition	supplemented with cycloheximide (50mg/l) and nalidixic acid (20mg/l)
[Ref.: #65500]	Enrichment culture duration	28 days
[Ref.: #65500]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64945]	Lechevalieria rhizosphaerae strain NEAU-A2 16S ribosomal RNA gene, partial sequence	KY744941	1504	ENA	2041025 tax ID

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GC-content

68.2 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

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Culture collection no.

DSM 104541, CGMCC 4.7405

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SI-ID 405219

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64945

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104541

#65500

Junwei Zhao, Wenchao Li, Linlin Shi, Han Wang, Ying Wang, Yue Zhao, Wensheng Xiang and Xiangjing Wang: Lechevalieria rhizosphaerae sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Lechevalieria. IJSEM 67: 4655 - 4659 2017 ( DOI 10.1099/ijsem.0.002351 , PubMed 28950934 )

#111103

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405219.1 )

* These data were automatically processed and therefore are not curated

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