Name: Izhakiella australiensis D4N98
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158282

Type strain:

Species: Izhakiella australiensis

Strain Designation: D4N98

Culture col. no.: DSM 105030, LMG 30066

Strain history: <- J. Mukan; School of Biotechnology, University of New South Wales, Randwick, Australia; D4N98

NCBI tax ID(s): 1926881 (species)

SI-ID 403945

DSM 105030

Section

Izhakiella australiensis D4N98 is a facultative anaerobe, chemoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from desert soil.

colony-forming
Gram-negative
chemoheterotroph
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Izhakiella*
	Species	**Izhakiella australiensis**
	Full Scientific Name (LPSN)	Izhakiella australiensis Ji et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65404]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.941

[Ref.: #65404]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %98.285

[Ref.: #65404]	Colony color	colourless
[Ref.: #65404]	Colony shape	circular
[Ref.: #65404]	Incubation period	1 day
[Ref.: #65404]	Cultivation medium used	LB agar

Multimedia content

[Ref.: #64942]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64942]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #64942]

Culture medium growth

[Ref.: #64942]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64942]

Culture medium composition

expand / minimize

[Ref.: #65404]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #65404]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #64942]		growth	28 °C
[Ref.: #65404]		growth	10-37 °C
[Ref.: #65404]	no	growth	40 °C
[Ref.: #65404]		optimum	25-30 °C

[Ref.: #64942]	Temperature range	mesophilic
[Ref.: #65404]	Temperature range	thermophilic
[Ref.: #65404]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65404]

Oxygen tolerance

facultative anaerobe

[Ref.: #65404]

Nutrition type

chemoheterotroph

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.714
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

[Ref.: #65404]	Observation	growth is observed on MacConkey agar, but no colour change is observed
[Ref.: #65404]	Observation	dominant polar lipids are phospholipids including phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a neutral lipid and a glycolipid

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65404]	amygdalin ChEBI	-	builds acid from
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #65404]	arabinose ChEBI	+	builds acid from
[Ref.: #65404]	arbutin ChEBI	-	builds acid from
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #65404]	cellobiose ChEBI	-	builds acid from
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #65404]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65404]	D-arabitol ChEBI	+	builds acid from
[Ref.: #65404]	D-fructose ChEBI	+	builds acid from
[Ref.: #65404]	D-fucose ChEBI	-	builds acid from
[Ref.: #65404]	D-galactose ChEBI	+	builds acid from
[Ref.: #65404]	D-glucose ChEBI	+	assimilation
[Ref.: #65404]	D-glucose ChEBI	+	builds acid from
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #65404]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65404]	D-mannitol ChEBI	+	assimilation
[Ref.: #65404]	D-mannitol ChEBI	+	builds acid from
[Ref.: #65404]	D-mannitol ChEBI	+	fermentation
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #65404]	D-mannose ChEBI	+	assimilation
[Ref.: #65404]	D-mannose ChEBI	+	builds acid from
[Ref.: #65404]	D-ribose ChEBI	+	builds acid from
[Ref.: #65404]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65404]	D-tagatose ChEBI	+	builds acid from
[Ref.: #65404]	erythritol ChEBI	+	builds acid from
[Ref.: #65404]	esculin ferric citrate	-	builds acid from
[Ref.: #65404]	galactitol ChEBI	-	builds acid from
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #65404]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65404]	glucose ChEBI	+	fermentation
[Ref.: #65404]	glycerol ChEBI	+	builds acid from
[Ref.: #65404]	glycogen ChEBI	-	builds acid from
[Ref.: #65404]	inulin ChEBI	-	builds acid from
[Ref.: #65404]	isocitrate ChEBI	-	carbon source
[Ref.: #65404]	L-arabinose ChEBI	-	fermentation
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #65404]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65404]	L-fucose ChEBI	-	builds acid from
[Ref.: #65404]	L-rhamnose ChEBI	+	assimilation
[Ref.: #65404]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #65404]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65404]	lactose ChEBI	-	builds acid from
[Ref.: #65404]	lactose ChEBI	-	fermentation
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #65404]	malate ChEBI	+	assimilation
[Ref.: #65404]	maltose ChEBI	-	builds acid from
[Ref.: #65404]	melezitose ChEBI	-	builds acid from
[Ref.: #65404]	melibiose ChEBI	-	builds acid from
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #65404]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65404]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65404]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65404]	myo-inositol ChEBI	-	fermentation
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #65404]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65404]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65404]	nitrate ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #65404]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65404]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65404]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65404]	potassium gluconate ChEBI	+	assimilation
[Ref.: #65404]	raffinose ChEBI	-	builds acid from
[Ref.: #65404]	ribitol ChEBI	-	builds acid from
[Ref.: #65404]	salicin ChEBI	-	builds acid from
[Ref.: #65404]	sorbitol ChEBI	-	fermentation
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #65404]	starch ChEBI	-	builds acid from
[Ref.: #65404]	sucrose ChEBI	-	builds acid from
[Ref.: #65404]	sucrose ChEBI	-	fermentation
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #65404]	sulfide ChEBI	-	reduction
[Ref.: #65404]	trehalose ChEBI	+	builds acid from
[Ref.: #65404]	trehalose ChEBI	+	fermentation
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #65404]	turanose ChEBI	-	builds acid from
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #65404]	xylitol ChEBI	-	builds acid from
[Ref.: #65404]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65404]	urea ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #65404]	acetoin ChEBI	+
[Ref.: #68368]	acetoin ChEBI	+		* from API 20E
[Ref.: #68368]	indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #65404]	acid phosphatase	+	3.1.3.2
[Ref.: #65404]	arginine dihydrolase	+	3.5.3.6
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #65404]	beta-galactosidase	+	3.2.1.23
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #65404]	catalase	+	1.11.1.6
[Ref.: #65404]	cystine arylamidase	+	3.4.11.3
[Ref.: #65404]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #65404]	gelatinase	-
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #65404]	lysine decarboxylase	+	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #65404]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65404]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #65404]	tryptophan deaminase	-	4.1.99.1
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #64942]	17725	+	-	+	-	-	-	-	-	-	+	-	+	+	-	-	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64942]	Sample type/isolated from	desert soil
[Ref.: #64942]	Geographic location (country and/or sea, region)	near Glendambo (S 31° 1' 54'', E 135° 48' 24.48'')
[Ref.: #64942]	Country	Australia
[Ref.: #64942]	Country ISO 3 Code	AUS
[Ref.: #64942]	Continent	Australia and Oceania
[Ref.: #64942]	Geographic location	-31.0317°/135.8070°

[Ref.: #65404]	Sample type/isolated from	an Australian desert soil
[Ref.: #65404]	Country	Australia
[Ref.: #65404]	Country ISO 3 Code	AUS
[Ref.: #65404]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Desert
#Environmental	#Terrestrial	#Soil
#Condition	#Xerophilic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY744694 (>99% sequence identity) for Izhakiella australiensis subclade from Microbeatlas

Aquatic Samples	13
Soil Samples	13
Animal Samples	116
Plant Samples	24
Total Samples	166

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64942]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64942]	Izhakiella australiensis strain D4N98 16S ribosomal RNA gene, partial sequence	KY744694	1531	ENA	1926881 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Izhakiella australiensis D4N98	GCA_002006995	scaffold	GenBank	1926881 tax ID	*
[Ref.: #66792]	Izhakiella australiensis D4N98	2791354900	draft	JGI IMG/M	1926881 tax ID	*

[Ref.: #64942]

GC-content

54.03 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Izhakiella australiensis D4N98 NCBI: GCA_002006995.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Izhakiella australiensis D4N98 NCBI: GCA_002006995.1
[Ref.: #69480]	flagellated	no	68.55	yes
[Ref.: #69480]	gram-positive	no	98.257	no
[Ref.: #69480]	anaerobic	no	97.443	yes
[Ref.: #69480]	aerobic	no	62.803	yes
[Ref.: #69480]	halophile	no	91.141	no
[Ref.: #69480]	spore-forming	no	94.158	no
[Ref.: #69480]	glucose-util	yes	95.656	yes
[Ref.: #69480]	thermophile	no	99.106	no
[Ref.: #69480]	motile	yes	91.749	no
[Ref.: #69480]	glucose-ferment	yes	89.639	yes

Availability in culture collections External links

[Ref.: #64942]

Culture collection no.

DSM 105030, LMG 30066

[Ref.: #111100]

SI-ID 403945

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Izhakiella australiensis sp. nov. isolated from an Australian desert soil.	Ji M, Tang S, Ferrari BC	Int J Syst Evol Microbiol	10.1099/ijsem.0.002171	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64942

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105030

#65404

Mukan Ji, Sihui Tang, Belinda C. Ferrari: Izhakiella australiensis sp. nov. isolated from an Australian desert soil. IJSEM 67: 4317 - 4322 2017 ( DOI 10.1099/ijsem.0.002171 , PubMed 29130429 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111100

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403945.1 )

* These data were automatically processed and therefore are not curated

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