Polynucleobacter aenigmaticus (DSM 24006, LMG 29706, MWH-K35W1)

Name: Polynucleobacter aenigmaticus (DSM 24006, LMG 29706, MWH-K35W1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158281

Type strain

Strain Designation MWH-K35W1

Culture col. no. DSM 24006 LMG 29706 MWH-K35W1

NCBI tax ID(s) 1743164

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Polynucleobacter aenigmaticus MWH-K35W1 is an aerobe, chemoorganoheterotroph, mesophilic prokaryote that forms circular colonies and was isolated from permanently anoxic water layer of a meromictic lake .

rod-shaped colony-forming aerobe chemoorganoheterotroph mesophilic genome sequence

Name and taxonomic classification

SI-ID 402347

LMG 29706,DSM 24006

@ref 20215

Last LPSN update 2025-12-16 10:07:59

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Polynucleobacter

Species Polynucleobacter aenigmaticus

Full scientific name Polynucleobacter aenigmaticus Hahn et al. 2017

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Gram stain	Confidence
65280	0.5-1 µm	0.3-0.5 µm	rod-shaped
125438				negative	95.828
125439				negative	99.9

Colony morphology

@ref	Colony shape	Medium used
65280	circular	nutrient-broth-soytone-yeast-extract medium

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
64941	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
65280	nutrient-broth-soytone-yeast-extract medium

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
64941	positive	growth	28	mesophilic
65280	positive	growth	5-32

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65280	aerobe
125439	obligate aerobe	95.2

Nutrition type

65280

Typechemoorganoheterotroph

Spore formation

@ref	Spore formation	Confidence
125438		93.548
125439		99.9

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65280	NaCl	positive	growth	0-0.3 %(w/v)

Observation

@ref	Observation
65280	The locus tag of the 16S rRNA gene is CBI30_02030.
65280	no anaerobic growth detected, but gene content of the type strain suggests an ability for anaerobic respiration.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65280	39150 ChEBI	4-oxopentanoate	-	assimilation
65280	30089 ChEBI	acetate	+	assimilation
65280	17750 ChEBI	betaine	-	assimilation
65280	16947 ChEBI	citrate	-	assimilation
65280	15824 ChEBI	D-fructose	-	assimilation
65280	12936 ChEBI	D-galactose	-	assimilation
65280	18024 ChEBI	D-galacturonic acid	+	assimilation
65280	62318 ChEBI	D-lyxose	+	assimilation
65280	16024 ChEBI	D-mannose	-	assimilation
65280	17924 ChEBI	D-sorbitol	-	assimilation
65280	29806 ChEBI	fumarate	+	assimilation
65280	29805 ChEBI	glycolate	-	assimilation
65280	36655 ChEBI	glyoxylate	-	assimilation
65280	16977 ChEBI	L-alanine	+	assimilation
65280	17196 ChEBI	L-asparagine	-	assimilation
65280	29991 ChEBI	L-aspartate	-	assimilation
65280	17561 ChEBI	L-cysteine	+	assimilation
65280	18287 ChEBI	L-fucose	-	assimilation
65280	29985 ChEBI	L-glutamate	+	assimilation
65280	15971 ChEBI	L-histidine	-	assimilation
65280	15603 ChEBI	L-leucine	-	assimilation
65280	17115 ChEBI	L-serine	+	assimilation
65280	25115 ChEBI	malate	+	assimilation
65280	15792 ChEBI	malonate	+	assimilation
65280	30623 ChEBI	oxalate	+	assimilation
65280	16452 ChEBI	oxaloacetate	-	assimilation
65280	17272 ChEBI	propionate	+	assimilation
65280	15361 ChEBI	pyruvate	+	assimilation
65280	30031 ChEBI	succinate	+	assimilation

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture temperature	Isolation procedure
64941	permanently anoxic water layer of a meromictic lake (monimolimnion)	Lake Krottensee, near St. Gilgen	Austria	AUT	Europe
65280	anoxic water sample from the monimolimnion in a depth of 35 m	meromictic Lake Krottensee in the Salzkammergut lake distric	Austria	AUT	Europe	6	This sample was characterized by a temperature of 6°C, a pH of 7.6, conductivity of 431 µS cm-1 and a lack of dissolved oxygen. The strain was isolated by using the filtration-acclimatization method and nutrient-broth-soytone-yeast-extract medium under aerobic conditions.

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64941	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM220663v1 assembly for Polynucleobacter aenigmaticus MWH-K35W1	contig	GCA_002206635	1743164.3	2675903126	1743164	66.43

GC content

@ref	GC-content (mol%)	Method
64941	45.98	sequence analysis
65280	45.98	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Polynucleobacter aenigmaticus MWH-K35W1
NCBI: GCA_002206635

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.90	no
125439	motility	BacteriaNetⓘ	yes	86.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Polynucleobacter sp. MWH-K35W1 strain MWH-K35W1
PATRIC: 1743164.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	95.83	no
125438	anaerobic	anaerobicⓘ	no	91.41	no
125438	spore-forming	spore-formingⓘ	no	93.55	no
125438	aerobic	aerobicⓘ	yes	80.76	yes
125438	thermophilic	thermophileⓘ	no	92.12	no
125438	flagellated	motile2+ⓘ	yes	66.27	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polynucleobacter aenigmaticus sp. nov. isolated from the permanently anoxic monimolimnion of a temperate meromictic lake.	Hahn MW, Koll U, Karbon G, Schmidt J, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002347	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64941	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24006
#65280	Martin W. Hahn, Ulrike Koll, Gerlinde Karbon, Johanna Schmidt, Elke Lang: Polynucleobacter aenigmaticus sp. nov. isolated from the permanently anoxic monimolimnion of a temperate meromictic lake. IJSEM 67: 4646 - 4654 2017 ( DOI 10.1099/ijsem.0.002347 , PubMed 29022553 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Polynucleobacter aenigmaticus (DSM 24006, LMG 29706, MWH-K35W1)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Observation

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002206635

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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