Name: Polynucleobacter aenigmaticus MWH-K35W1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158281

Type strain:

Species: Polynucleobacter aenigmaticus

Strain Designation: MWH-K35W1

Culture col. no.: DSM 24006, LMG 29706

Strain history: <- M. W. Hahn, Austrian Academy of Sci., Institute of Limnology, Mondsee; MWH-K35W1

NCBI tax ID(s): 1743164 (species)

SI-ID 402347

DSM 24006

Section

Polynucleobacter aenigmaticus MWH-K35W1 is an aerobe, chemoorganoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from permanently anoxic water layer of a meromictic lake .

colony-forming
rod-shaped
aerobe
chemoorganoheterotroph
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Polynucleobacter*
	Species	**Polynucleobacter aenigmaticus**
	Full Scientific Name (LPSN)	Polynucleobacter aenigmaticus Hahn et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65280]	Cell length	0.5-1 µm

[Ref.: #65280]	Cell width	0.3-0.5 µm

[Ref.: #65280]	Cell shape	rod-shaped

[Ref.: #65280]	Colony shape	circular
[Ref.: #65280]	Cultivation medium used	nutrient-broth-soytone-yeast-extract medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64941]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #64941]

Culture medium growth

[Ref.: #64941]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64941]

Culture medium composition

expand / minimize

[Ref.: #65280]

Culture medium

nutrient-broth-soytone-yeast-extract medium

[Ref.: #65280]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #64941]			growth	28 °C
[Ref.: #65280]			growth	5-32 °C

[Ref.: #64941]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65280]

Oxygen tolerance

aerobe

[Ref.: #65280]

Nutrition type

chemoorganoheterotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65280]		NaCl		growth	0-0.3	%(w/v)

[Ref.: #65280]	Observation	The locus tag of the 16S rRNA gene is CBI30_02030.
[Ref.: #65280]	Observation	no anaerobic growth detected, but gene content of the type strain suggests an ability for anaerobic respiration.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65280]	4-oxopentanoate ChEBI	-	assimilation
[Ref.: #65280]	acetate ChEBI	+	assimilation
[Ref.: #65280]	betaine ChEBI	-	assimilation
[Ref.: #65280]	citrate ChEBI	-	assimilation
[Ref.: #65280]	D-fructose ChEBI	-	assimilation
[Ref.: #65280]	D-galactose ChEBI	-	assimilation
[Ref.: #65280]	D-galacturonic acid ChEBI	+	assimilation
[Ref.: #65280]	D-lyxose ChEBI	+	assimilation
[Ref.: #65280]	D-mannose ChEBI	-	assimilation
[Ref.: #65280]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65280]	fumarate ChEBI	+	assimilation
[Ref.: #65280]	glycolate ChEBI	-	assimilation
[Ref.: #65280]	glyoxylate ChEBI	-	assimilation
[Ref.: #65280]	L-alanine ChEBI	+	assimilation
[Ref.: #65280]	L-asparagine ChEBI	-	assimilation
[Ref.: #65280]	L-aspartate ChEBI	-	assimilation
[Ref.: #65280]	L-cysteine ChEBI	+	assimilation
[Ref.: #65280]	L-fucose ChEBI	-	assimilation
[Ref.: #65280]	L-glutamate ChEBI	+	assimilation
[Ref.: #65280]	L-histidine ChEBI	-	assimilation
[Ref.: #65280]	L-leucine ChEBI	-	assimilation
[Ref.: #65280]	L-serine ChEBI	+	assimilation
[Ref.: #65280]	malate ChEBI	+	assimilation
[Ref.: #65280]	malonate ChEBI	+	assimilation
[Ref.: #65280]	oxalate ChEBI	+	assimilation
[Ref.: #65280]	oxaloacetate ChEBI	-	assimilation
[Ref.: #65280]	propionate ChEBI	+	assimilation
[Ref.: #65280]	pyruvate ChEBI	+	assimilation
[Ref.: #65280]	succinate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64941]	Sample type/isolated from	permanently anoxic water layer of a meromictic lake (monimolimnion)
[Ref.: #64941]	Geographic location (country and/or sea, region)	Lake Krottensee, near St. Gilgen
[Ref.: #64941]	Country	Austria
[Ref.: #64941]	Country ISO 3 Code	AUT
[Ref.: #64941]	Continent	Europe

[Ref.: #65280]	Sample type/isolated from	anoxic water sample from the monimolimnion in a depth of 35 m
[Ref.: #65280]	Geographic location (country and/or sea, region)	meromictic Lake Krottensee in the Salzkammergut lake distric
[Ref.: #65280]	Country	Austria
[Ref.: #65280]	Country ISO 3 Code	AUT
[Ref.: #65280]	Continent	Europe
[Ref.: #65280]	Enrichment culture temperature	6 ̊C
[Ref.: #65280]	Isolation/enrichment procedure	This sample was characterized by a temperature of 6°C, a pH of 7.6, conductivity of 431 µS cm-1 and a lack of dissolved oxygen. The strain was isolated by using the filtration-acclimatization method and nutrient-broth-soytone-yeast-extract medium under aerobic conditions.
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Lake (large)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64941]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64941]	Polynucleobacter aenigmaticus strain MWH-K35W1 Ga0129263_102, whole genome shotgun sequence	CBI30_02030	369074	ENA	1743164 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Polynucleobacter aenigmaticus MWH-K35W1	GCA_002206635	contig	GenBank	1743164 tax ID	*
[Ref.: #66792]	Polynucleobacter sp. MWH-K35W1 strain MWH-K35W1	1743164.3	wgs	PATRIC	1743164 tax ID	*

[Ref.: #64941]	GC-content	45.98 mol%	sequence analysis
[Ref.: #65280]	GC-content	45.98 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Polynucleobacter sp. MWH-K35W1 strain MWH-K35W1 PATRIC: 1743164.3
[Ref.: #69480]	motile	yes	68.843	no
[Ref.: #69480]	gram-positive	no	98.399	no
[Ref.: #69480]	anaerobic	no	98.48	yes
[Ref.: #69480]	aerobic	yes	79.058	yes
[Ref.: #69480]	halophile	no	94.039	yes
[Ref.: #69480]	spore-forming	no	96.197	no
[Ref.: #69480]	thermophile	no	95.485	yes
[Ref.: #69480]	glucose-util	no	85.18	no
[Ref.: #69480]	flagellated	no	59.127	no
[Ref.: #69480]	glucose-ferment	no	90.698	no

Availability in culture collections External links

[Ref.: #64941]

Culture collection no.

DSM 24006, LMG 29706

[Ref.: #111099]

SI-ID 402347

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polynucleobacter aenigmaticus sp. nov. isolated from the permanently anoxic monimolimnion of a temperate meromictic lake.	Hahn MW, Koll U, Karbon G, Schmidt J, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002347	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64941

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24006

#65280

Martin W. Hahn, Ulrike Koll, Gerlinde Karbon, Johanna Schmidt, Elke Lang: Polynucleobacter aenigmaticus sp. nov. isolated from the permanently anoxic monimolimnion of a temperate meromictic lake. IJSEM 67: 4646 - 4654 2017 ( DOI 10.1099/ijsem.0.002347 , PubMed 29022553 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#111099

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402347.1 )

* These data were automatically processed and therefore are not curated

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Polynucleobacter sp. MWH-K35W1 strain MWH-K35W1

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