Clostridium ammoniilyticum (DSM 108253, H4_15, JCM 34991)

Name: Clostridium ammoniilyticum (DSM 108253, H4_15, JCM 34991)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 157764

Type strain

Strain Designation H4_15

Culture col. no. DSM 108253 H4_15 JCM 34991

NCBI tax ID(s) 2981784

Special Collections DSMZ Sanger human gut JCM

History <- H. Browne, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom; H4_15 <- H. Browne {2014}

Links

Clostridium ammoniilyticum H4_15 is a bacterium that was isolated from human faeces.

genome sequence Bacteria

Name and taxonomic classification

SI-ID 398450

JCM 34991,DSM 108253

@ref 20215

Last LPSN update 2026-02-09 11:38:08

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium ammoniilyticum

Full scientific name Clostridium ammoniilyticum Hitch et al. 2022

BacDive ID	Other strains from **Clostridium ammoniilyticum** (2)	Type strain
157763	C. ammoniilyticum H6_14, DSM 108252
158461	C. ammoniilyticum H3_29, DSM 108213, JCM 34989

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
64336	YCFA-MEDIUM (MODIFIED) (DSMZ Medium 1611)		Medium recipe at MediaDive	Name: YCFA MEDIUM (modified) (DSMZ Medium 1611) Composition: Casitone 10.0 g/l Glucose 5.0 g/l NaHCO3 4.0 g/l Yeast extract 2.5 g/l Acetic acid 1.78695 g/l L-Cysteine HCl 1.0 g/l NaCl 0.9 g/l Propionic acid 0.62559 g/l KH2PO4 0.45 g/l K2HPO4 0.45 g/l CaCl2 x 2 H2O 0.09 g/l n-Valeric acid 0.08451 g/l iso-Valeric acid 0.0837899 g/l iso-Butyric acid 0.07695 g/l MgSO4 x 7 H2O 0.045 g/l Hemin 0.01 g/l Resazurin 0.001 g/l Pyridoxine hydrochloride 0.0001 g/l Nicotinic acid 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavin 5e-05 g/l D-Calcium pantothenate 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Lipoic acid 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Vitamin B12 1e-06 g/l Distilled water
64336	PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)		Medium recipe at MediaDive	Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
64336	positive	growth	37

Physiology and metabolism

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
64336	human faeces	Cambridgeshire, Hinxton	United Kingdom	GBR	Europe

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
64336	yes, in single cases	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2556690v1 assembly for [Clostridium] ammoniilyticum H4_15	scaffold	GCA_025566905	2981784.5	8111536372	2981784	69.52

GC content

@ref	GC-content (mol%)	Method
64336	29	sequence analysis

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium ammoniilyticum H4_15
NCBI: GCA_025566905.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	83.29	no
125438	anaerobic	anaerobicⓘ	yes	87.19	no
125438	aerobic	aerobicⓘ	no	97.53	no
125438	spore-forming	spore-formingⓘ	no	57.65	no
125438	thermophilic	thermophileⓘ	no	94.24	yes
125438	flagellated	motile2+ⓘ	no	85.71	no

Literature

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64336	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108253
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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