Lawsonibacter asaccharolyticus (DSM 106493, JCM 32166, KCTC 15743)

Name: Lawsonibacter asaccharolyticus (DSM 106493, JCM 32166, KCTC 15743)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 157758

Type strain

Strain Designation 3BBH22

Culture col. no. DSM 106493 JCM 32166 KCTC 15743 3BBH22

NCBI tax ID(s) 2108523

Special Collections DSMZ JCM KCTC Literature strains

History DSMZ <- M. Sakamoto, JCM; JCM 32166 <- M. Sakamoto, RIKEN; 3BBH22 JCM <-- M. Sakamoto; RIKEN BRC-JCM, Japan; 3BBH22. KCTC <- DSM <- M Sakamoto, JCM 32166 <- M Sakamoto, RIKEN, Wako, Saitama, Japan, 3BBH22

Links

Lawsonibacter asaccharolyticus 3BBH22 is an obligate anaerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from faeces of woman.

Gram-positive rod-shaped colony-forming obligate anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398672

JCM 32166,KCTC 15743,DSM 106493

@ref 20215

Last LPSN update 2026-05-29 08:43:48

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Oscillospiraceae

Genus Lawsonibacter

Species Lawsonibacter asaccharolyticus

Full scientific name Lawsonibacter asaccharolyticus Sakamoto et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
66569	positive	2.4-20 µm	0.8-1.5 µm	rod-shaped
67771				rod-shaped
67771	positive

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
66569	0.5-1 mm	grey to off-white-grey	circular	4 days	Brucella blood agar with haemin and menadione

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
64330	CHOPPED MEAT MEDIUM (DSMZ Medium 78)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
66569	Brucella blood agar with haemin and menadione
64330	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

Culture temp

@ref	Growth	Type	Temperature (°C)
64330	positive	growth	37
66569	positive	optimum	37
66569	positive	growth	30-45
67770	positive	growth	37
67771	positive	growth	37

Culture pH

@ref	Ability	Type	PH
66569	positive	optimum	7
66569	positive	growth	6-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
66569	obligate anaerobe
67771	anaerobe
125439	obligate anaerobe	99.609

Spore formation

@ref	Spore formation
66569
67771

Observation

@ref	Observation
66569	sporulation-specific gene spo0A is present
66569	No growth occurs on medium containing 20 % (w/v) bile.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
66569	4853 ChEBI	esculin	-	hydrolysis
66569	5291 ChEBI	gelatin	-	hydrolysis
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
66569	37684 ChEBI	mannose	-	fermentation
66569	17632 ChEBI	nitrate	-	reduction
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
66569	16243 ChEBI	quercetin	-	assimilation
66569	16634 ChEBI	raffinose	-	fermentation
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite
66569	16136 ChEBI	hydrogen sulfide
66569	35581 ChEBI	indole
68380	35581 ChEBI	indole	from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
66569	6-phospho-beta-galactosidase	-	3.2.1.85
66569	acid phosphatase	+	3.1.3.2
66569	alanine arylamidase	-	3.4.11.2
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
66569	alkaline phosphatase	+	3.1.3.1
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
66569	alpha-arabinosidase	-	3.2.1.55
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
66569	alpha-fucosidase	-	3.2.1.51
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
66569	alpha-galactosidase	-	3.2.1.22
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
66569	alpha-glucosidase	-	3.2.1.20
68380	alpha-glucosidase	-	3.2.1.20	from API rID32A
66569	alpha-mannosidase	-	3.2.1.24
66569	arginine arylamidase	+
66569	arginine dihydrolase	-	3.5.3.6
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
66569	beta-galactosidase	-	3.2.1.23
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
66569	beta-glucosidase	-	3.2.1.21
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
66569	beta-glucuronidase	-	3.2.1.31
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
66569	catalase	-	1.11.1.6
66569	chymotrypsin	-	3.4.4.5
66569	cystine arylamidase	-	3.4.11.3
66569	esterase	+
66569	esterase Lipase (C 8)	+
66569	glutamate decarboxylase	-	4.1.1.15
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
66569	glutamyl-glutamate arylamidase	-
68380	glutamyl-glutamate arylamidase	-		from API rID32A
66569	glycin arylamidase	-
68380	glycin arylamidase	-		from API rID32A
66569	histidine arylamidase	-
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
66569	leucine arylamidase	+	3.4.11.1
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
66569	leucyl glycin arylamidase	-	3.4.11.1
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
66569	lipase	-
66569	N-acetyl-beta-glucosaminidase	-	3.2.1.52
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
66569	naphthol-AS-BI-phosphohydrolase	+
66569	phenylalanine arylamidase	-
68380	phenylalanine arylamidase	-		from API rID32A
66569	proline-arylamidase	-	3.4.11.5
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
66569	pyroglutamic acid arylamidase	-
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
66569	serine arylamidase	+
68380	serine arylamidase	-		from API rID32A
66569	trypsin	-	3.4.21.4
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
66569	tyrosine arylamidase	+
68380	tyrosine arylamidase	-		from API rID32A
66569	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
66569	valine arylamidase	-

Fatty acid profile

Metadata FA analysis

@ref

66569

Fatty acid	Percentage	SD
C14:0	21.3	2.4
C16:0	19.6	6.9
C18:1 w9c	14.5	3.25
C16:0 DMA	12.9	5.2
C18:0	12.4	6.4
C18:2 w6,9c	12.2	4.5
C18:0 DMA	7.1	0.7
C14:0 DMA	2.6
C12:0	2.2

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
64330	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
64330	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Female

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
64330	faeces of woman	Homo sapiens	Japan	JPN	Asia	36.0239	140.121 36.0239/140.121
66569	faecal sample of a healthy Japanese woman (41 years old)	Homo sapiens						Brucella blood agar with haemin and menadione	2-4 days	37	The fresh faecal sample was plated onto Brucella blood agar with haemin and menadione (BB; JCM Medium no. 677). After 2-4 days of incubation at 37°C under a H2/CO2/N2 (1 : 1 : 8, by vol.) gas mixture
67770	Feces of a woman in the forties	Homo sapiens	Japan	JPN	Asia
67771	From a faecal sample of a healthy Japanese woman	Homo sapiens	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence LC371917 (>99% sequence identity) for Eubacteriales from Microbeatlas

Aquatic Samples	553
Soil Samples	346
Animal Samples	71245
Plant Samples	77
Total Samples	72221

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM311275v1 assembly for Lawsonibacter asaccharolyticus 3BBH22	contig	GCA_003112755	2108523.6	2831418171	2108523	46.38

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66569	Lawsonibacter asaccharolyticus 3BBH22 gene for 16S ribosomal RNA, partial sequence	LC371917	1481		2108523

GC content

@ref	GC-content (mol%)	Method
66569	60.2-62.2	genome sequence analysis
67771	61.2	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM311275v1 assembly for Lawsonibacter asaccharolyticus 3BBH22
NCBI: GCA_003112755

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	99.61	no
125439	gram_stain	BacteriaNetⓘ	negative	54.33	no
125439	motility	BacteriaNetⓘ	no	61.58	no
125439	spore_formation	BacteriaNetⓘ	no	67.29	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Lawsonibacter asaccharolyticus JCM 32166(T), a Butyrate-Producing Bacterium, Isolated from Human Feces.	Sakamoto M, Ikeyama N, Yuki M, Ohkuma M	Genome Announc	10.1128/genomeA.00563-18	2018
Phylogeny	Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces.	Sakamoto M, Iino T, Yuki M, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002800	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64330	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106493
#66569	Mitsuo Sakamoto, Takao Iino, Masahiro Yuki, Moriya Ohkuma: Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces. IJSEM 68: 2074 - 2081 2018 ( DOI 10.1099/ijsem.0.002800 , PubMed 29745868 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68380	Automatically annotated from API rID32A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Lawsonibacter asaccharolyticus (DSM 106493, JCM 32166, KCTC 15743)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003112755

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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