Name: Lawsonibacter asaccharolyticus 3BBH22
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 157758

Type strain:

Species: Lawsonibacter asaccharolyticus

Strain Designation: 3BBH22

Culture col. no.: DSM 106493, JCM 32166, KCTC 15743

Strain history: <- DSM <- M Sakamoto, JCM 32166 <- M Sakamoto, RIKEN, Wako, Saitama, Japan, 3BBH22

NCBI tax ID(s): 2108523 (species)

SI-ID 398672

DSM 106493

Special Collections

Section

Lawsonibacter asaccharolyticus 3BBH22 is an obligate anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from faeces of woman.

colony-forming
Gram-positive
rod-shaped
mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Oscillospiraceae*
	Genus	*Lawsonibacter*
	Species	**Lawsonibacter asaccharolyticus**
	Full Scientific Name (LPSN)	Lawsonibacter asaccharolyticus Sakamoto et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66569]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #66569]	Cell length	2.4-20 µm

[Ref.: #66569]	Cell width	0.8-1.5 µm

[Ref.: #66569]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #66569]	Colony size	0.5-1 mm
[Ref.: #66569]	Colony color	grey to off-white-grey
[Ref.: #66569]	Colony shape	circular
[Ref.: #66569]	Incubation period	4 days
[Ref.: #66569]	Cultivation medium used	Brucella blood agar with haemin and menadione

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64330]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #64330]

Culture medium growth

[Ref.: #64330]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64330]

Culture medium composition

expand / minimize

[Ref.: #66569]

Culture medium

Brucella blood agar with haemin and menadione

[Ref.: #66569]

Culture medium growth

[Ref.: #64330]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #64330]

Culture medium growth

[Ref.: #64330]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #64330]	growth	37 °C
[Ref.: #66569]	optimum	37 °C
[Ref.: #66569]	growth	30-45 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #67771]	growth	37 °C

	pH	Kind of pH		pH
[Ref.: #66569]			optimum	7
[Ref.: #66569]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66569]	Oxygen tolerance	obligate anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #66569]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no

[Ref.: #66569]	Observation	sporulation-specific gene spo0A is present
[Ref.: #66569]	Observation	No growth occurs on medium containing 20 % (w/v) bile.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #66569]	esculin ChEBI	-	hydrolysis
[Ref.: #66569]	gelatin ChEBI	-	hydrolysis
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #66569]	mannose ChEBI	-	fermentation
[Ref.: #66569]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #66569]	quercetin ChEBI	-	assimilation
[Ref.: #66569]	raffinose ChEBI	-	fermentation
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #66569]	hydrogen sulfide ChEBI	no
[Ref.: #66569]	indole ChEBI	no
[Ref.: #68380]	indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #66569]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #66569]	acid phosphatase	+	3.1.3.2
[Ref.: #66569]	alanine arylamidase	-	3.4.11.2
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #66569]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #66569]	alpha-arabinosidase	-	3.2.1.55
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #66569]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #66569]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #66569]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #66569]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66569]	arginine arylamidase	+
[Ref.: #66569]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #66569]	beta-galactosidase	-	3.2.1.23
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #66569]	beta-glucosidase	-	3.2.1.21
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #66569]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #66569]	catalase	-	1.11.1.6
[Ref.: #66569]	chymotrypsin	-	3.4.4.5
[Ref.: #66569]	cystine arylamidase	-	3.4.11.3
[Ref.: #66569]	esterase	+
[Ref.: #66569]	esterase Lipase (C 8)	+
[Ref.: #66569]	glutamate decarboxylase	-	4.1.1.15
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #66569]	glutamyl-glutamate arylamidase	-
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #66569]	glycin arylamidase	-
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #66569]	histidine arylamidase	-
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #66569]	leucine arylamidase	+	3.4.11.1
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #66569]	leucyl glycin arylamidase	-	3.4.11.1
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #66569]	lipase	-
[Ref.: #66569]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #66569]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66569]	phenylalanine arylamidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #66569]	proline-arylamidase	-	3.4.11.5
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #66569]	pyroglutamic acid arylamidase	-
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #66569]	serine arylamidase	+
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #66569]	trypsin	-	3.4.21.4
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #66569]	tyrosine arylamidase	+
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #66569]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #66569]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66569]

Fatty acid	Percentage	SD
C14:0	21.3	2.4
C16:0	19.6	6.9
C18:1 w9c	14.5	3.25
C16:0 DMA	12.9	5.2
C18:0	12.4	6.4
C18:2 w6,9c	12.2	4.5
C18:0 DMA	7.1	0.7
C14:0 DMA	2.6
C12:0	2.2

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #64330]	14762	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #64330]	14761	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64330]	Sample type/isolated from	faeces of woman
[Ref.: #64330]	Country	Japan
[Ref.: #64330]	Country ISO 3 Code	JPN
[Ref.: #64330]	Continent	Asia
[Ref.: #64330]	Geographic location	36.0239°/140.1210°

[Ref.: #66569]	Sample type/isolated from	faecal sample of a healthy Japanese woman (41 years old)
[Ref.: #66569]	Enrichment culture	Brucella blood agar with haemin and menadione
[Ref.: #66569]	Enrichment culture duration	2-4 days
[Ref.: #66569]	Enrichment culture temperature	37 ̊C
[Ref.: #66569]	Isolation/enrichment procedure	The fresh faecal sample was plated onto Brucella blood agar with haemin and menadione (BB; JCM Medium no. 677). After 2-4 days of incubation at 37°C under a H2/CO2/N2 (1 : 1 : 8, by vol.) gas mixture

[Ref.: #67770]	Sample type/isolated from	Feces of a woman in the forties
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From a faecal sample of a healthy Japanese woman
[Ref.: #67771]	Country	Japan
[Ref.: #67771]	Country ISO 3 Code	JPN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Female

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC371917 (>99% sequence identity) for Eubacteriales from Microbeatlas

Aquatic Samples	553
Soil Samples	346
Animal Samples	71245
Plant Samples	77
Total Samples	72221

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66569]	Lawsonibacter asaccharolyticus 3BBH22 gene for 16S ribosomal RNA, partial sequence	LC371917	1481	GenBank ENA	2108523 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lawsonibacter asaccharolyticus 3BBH22	GCA_003112755	contig	GenBank ENA	2108523 tax ID
[Ref.: #66792]	Lawsonibacter asaccharolyticus 3BBH22	2831418171	draft	JGI IMG/M	2108523 tax ID	*

[Ref.: #66569]	GC-content	60.2-62.2 mol%	genome sequence analysis
[Ref.: #67771]	GC-content	61.2 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #64330]

Culture collection no.

DSM 106493, JCM 32166, KCTC 15743

[Ref.: #110618]

SI-ID 398672

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Lawsonibacter asaccharolyticus JCM 32166(T), a Butyrate-Producing Bacterium, Isolated from Human Feces.	Sakamoto M, Ikeyama N, Yuki M, Ohkuma M	Genome Announc	10.1128/genomeA.00563-18	2018	*
Phylogeny	Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces.	Sakamoto M, Iino T, Yuki M, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002800	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64330

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106493

#66569

Mitsuo Sakamoto, Takao Iino, Masahiro Yuki, Moriya Ohkuma: Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces. IJSEM 68: 2074 - 2081 2018 ( DOI 10.1099/ijsem.0.002800 , PubMed 29745868 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#110618

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398672.1 )

* These data were automatically processed and therefore are not curated

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