Name: Gilliamella apis NO3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 157587

Type strain:

Species: Gilliamella apis

Strain Designation: NO3

Culture col. no.: DSM 105629, LMG 30293

Strain history: <- J. Ludvigsen, Norwegian Univ. of Science and Technol., Trondheim, Norway; NO3

NCBI tax ID(s): 1970738 (species)

SI-ID 402083

DSM 105629

Section

Gilliamella apis NO3 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from honeybee gut.

Gram-negative
rod-shaped
anaerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Orbales*
	Family	*Orbaceae*
	Genus	*Gilliamella*
	Species	**Gilliamella apis**
	Full Scientific Name (LPSN)	Gilliamella apis Ludvigsen et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65477]	Gram stain	negative

[Ref.: #65477]	Cell length	1-0.25 µm

[Ref.: #65477]	Cell shape	rod-shaped

[Ref.: #65477]	Colony size	<1 mm
[Ref.: #65477]	Incubation period	2 days
[Ref.: #65477]	Cultivation medium used	Trypticase soy agar + 5% horse blood

[Ref.: #65477]	Colony size	<1 mm
[Ref.: #65477]	Cultivation medium used	HIA + 5 % horse blood

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BHI MEDIUM (DSMZ Medium 215)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Trypticase soy agar

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

HIA

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Culture medium growth

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Culture medium

Trypticase soy agar + 5% horse blood

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Culture medium growth

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Culture medium

HIA + 5 % horse blood

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #64156]			growth	37 °C

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Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

anaerobe

[Ref.: #65477]	Observation	harbours the genes xylA, xylB, rhaA, rhaB and rhaD for xylene and rhamnose catabolism
[Ref.: #65477]	Observation	harbours the Tet H determinant that confers resistance towards tetracycline
[Ref.: #65477]	Observation	polar lipids are phosphoaminoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65477]	D-fructose ChEBI	+	fermentation
[Ref.: #65477]	D-glucose ChEBI	+	fermentation
[Ref.: #65477]	sucrose ChEBI	+	fermentation

	Metabolite	Production
[Ref.: #65477]	acetoin ChEBI	yes
[Ref.: #65477]	D-mannitol ChEBI	no
[Ref.: #65477]	D-mannose ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65477]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65477]	beta-galactosidase	-	3.2.1.23
[Ref.: #65477]	catalase	-	1.11.1.6
[Ref.: #65477]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65477]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #65477]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64156]	Sample type/isolated from	honeybee gut
[Ref.: #64156]	Geographic location (country and/or sea, region)	Aas
[Ref.: #64156]	Country	Norway
[Ref.: #64156]	Country ISO 3 Code	NOR
[Ref.: #64156]	Continent	Europe
[Ref.: #64156]	Geographic location	59.6644°/10.7944°

[Ref.: #65477]	Sample type/isolated from	homogenized guts collected from honeybees
[Ref.: #65477]	Geographic location (country and/or sea, region)	Ås
[Ref.: #65477]	Country	Norway
[Ref.: #65477]	Country ISO 3 Code	NOR
[Ref.: #65477]	Continent	Europe
[Ref.: #65477]	Isolation/enrichment procedure	isolated after 48 h of incubation at 37°C, in an enhanced CO2 atmosphere (BD GasPak EZ CO2 Container system, Becton Dickinson; CO2 2.5 %), on tryptic soy agar (TSA) supplemented with 5 % horse blood and 12µg tetracycline ml^-1
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Gilliamella apis NO3	GCA_002142155	contig	GenBank	1970738 tax ID	*
[Ref.: #66792]	Gilliamella apis NO3	2834098943	draft	JGI IMG/M	1970738 tax ID	*

Availability in culture collections External links

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Culture collection no.

DSM 105629, LMG 30293

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SI-ID 402083

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Addressing the diversity of the honeybee gut symbiont Gilliamella: description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera).	Ludvigsen J, Porcellato D, Amdam GV, Rudi K	Int J Syst Evol Microbiol	10.1099/ijsem.0.002749	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64156

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105629

#65477

Jane Ludvigsen, Davide Porcellato, Gro V. Amdam, Knut Rudi: Addressing the diversity of the honeybee gut symbiont Gilliamella: description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera). IJSEM 68: 1762 - 1770 2018 ( DOI 10.1099/ijsem.0.002749 , PubMed 29624166 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#110456

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402083.1 )

* These data were automatically processed and therefore are not curated

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