Name: Kitasatospora purpeofusca H-5080
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 15498

Type strain:

Species: Kitasatospora purpeofusca

Strain Designation: H-5080

Culture col. no.: DSM 40283, ATCC 23952, CBS 935.68, IFO 12905, ISP 5283, KCC S-0156, KCC S-0509, NBRC 12905, NRRL B-1817, RIA 1197, JCM 4156, BCRC 12093, CGMCC 4.1767, CGMCC 4.1999, JCM 4665, KCTC 19967, NCIMB 9822, NRRL ISP-5283

Strain history: KCC S-0156 <-- G. Nakamura <-- H. Yonehara (IAM 0073) <-- T. Yamaguchi H-5080.

NCBI tax ID(s): 67352 (species)

SI-ID 264282

ATCC 23952, IFO 12905, DSM 40283, NRRL B-1817, JCM 4156, CCRC 12093, NCIMB 9822, JCM 4665, IAM0073, VKM Ac-1825, CBS 935.68, NBRC 12905, BCRC 12093, KCTC 19967, LMG 20283, NCIB 9822, CGMCC 4.1767, CGMCC 4.1999

Section

Kitasatospora purpeofusca H-5080 is a spore-forming, mesophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from soil.

antibiotic compound production
Gram-positive
spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Kitasatospora*
	Species	**Kitasatospora purpeofusca**
	Full Scientific Name (LPSN)	Kitasatospora purpeofusca (Yamaguchi and Saburi 1955) Labeda et al. 2017

Synonym

Streptomyces purpeofuscus

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #9434]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #9434]

Culture medium growth

[Ref.: #9434]

Culture medium link

Medium recipe at MediaDive

[Ref.: #9434]

Culture medium composition

expand / minimize

[Ref.: #9434]

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

[Ref.: #9434]

Culture medium growth

[Ref.: #9434]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #9434]	growth	28 °C
[Ref.: #18579]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #9434]	Temperature range	mesophilic
[Ref.: #18579]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #9434]	Name of produced compound	negamycin
[Ref.: #9434]	Name of produced compound	anti-trichomonas antibiotic
[Ref.: #20216]	Name of produced compound	Nagemycin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18579]		NaCl		maximum	2.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18579]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18579]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #18579]	fructose ChEBI	-
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18579]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #18579]	mannitol ChEBI	-
[Ref.: #18579]	myo-inositol ChEBI	-
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18579]	raffinose ChEBI	-
[Ref.: #18579]	rhamnose ChEBI	-
[Ref.: #18579]	sucrose ChEBI	-
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
[Ref.: #18579]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18579]	137	-	-	-	-	-	-	+	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9434]	Sample type/isolated from	soil
[Ref.: #9434]	Country	Japan
[Ref.: #9434]	Country ISO 3 Code	JPN
[Ref.: #9434]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ781364 (>99% sequence identity) for Kitasatospora from Microbeatlas

Aquatic Samples	19
Soil Samples	1166
Animal Samples	107
Plant Samples	99
Total Samples	1391

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18579]	Biosafety level	1	German classification
[Ref.: #9434]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #9434]	Streptomyces purpeofuscus 16S rRNA gene, type strain LMG 20283	AJ781364	1476	ENA	67352 tax ID
[Ref.: #20218]	Streptomyces purpeofuscus gene for 16S rRNA, partial sequence	AB122757	564	ENA	67352 tax ID	*
[Ref.: #20218]	Streptomyces purpeofuscus strain IFO 12905 16S ribosomal RNA gene, partial sequence	AY999863	1420	ENA	67352 tax ID	*
[Ref.: #20218]	Streptomyces purpeofuscus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4665	D44294	121	ENA	67352 tax ID	*
[Ref.: #20218]	Streptomyces purpeofuscus gene for 16S rRNA, partial sequence, strain: NBRC 12905	AB184234	1451	ENA	67352 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Kitasatospora purpeofusca NRRL ISP-5283	GCA_000716225	scaffold	GenBank	67352 tax ID
[Ref.: #67770]	Kitasatospora purpeofusca NRRL B-1817	GCA_000718025	contig	GenBank	67352 tax ID

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Kitasatospora purpeofusca NRRL ISP-5283 NCBI: GCA_000716225.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cellulomonas sp. TKZ-21 PATRIC: 2071633.3
[Ref.: #69480]	motile	yes	78.585	no
[Ref.: #69480]	gram-positive	yes	91.529	no
[Ref.: #69480]	anaerobic	no	99.064	no
[Ref.: #69480]	aerobic	yes	91.451	no
[Ref.: #69480]	halophile	no	94.75	no
[Ref.: #69480]	spore-forming	no	83.19	no
[Ref.: #69480]	glucose-util	yes	85.087	no
[Ref.: #69480]	flagellated	no	91.307	no
[Ref.: #69480]	thermophile	no	98.705	yes
[Ref.: #69480]	glucose-ferment	no	66.54	no

Availability in culture collections External links

[Ref.: #9434]

Culture collection no.

DSM 40283, ATCC 23952, CBS 935.68, IFO 12905, ISP 5283, KCC S-0156, KCC S-0509, NBRC 12905, NRRL B-1817, RIA 1197, JCM 4156, BCRC 12093, CGMCC 4.1767, CGMCC 4.1999, JCM 4665, KCTC 19967, NCIMB 9822, NRRL ISP-5283

[Ref.: #84584]

SI-ID 264282

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kitasatospora acidiphila sp. nov., isolated from pine grove soil, exhibiting antimicrobial potential.	Kim MJ, Roh SG, Kim MK, Park C, Kim S, Kim SB	Int J Syst Evol Microbiol	10.1099/ijsem.0.004450	2020	*

References

#9434

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40283

#18579

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#84584

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID264282.1 )

* These data were automatically processed and therefore are not curated

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Kitasatospora purpeofusca NRRL ISP-5283

Cellulomonas sp. TKZ-21

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