Streptomyces pactus (VKM Ac-1911, NCIMB 9445, DSM 40530)

Name: Streptomyces pactus (VKM Ac-1911, NCIMB 9445, DSM 40530)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15452

Type strain

Culture col. no. VKM Ac-1911 NCIMB 9445 DSM 40530 ATCC 27456 CBS 461.69 19 more

NCBI tax ID(s) 68249

Special Collections DSMZ JCM KCTC Wink compendium

History <- E.B. Shirling, ISP <- NRRL <- Upjohn Co., UC 2432 KCC S-0288 <-- NCIB 9445 <-- Upjohn Co., USA; UC 2432.

Links

Streptomyces pactus DSM 40530 is a spore-forming, mesophilic prokaryote that builds an aerial mycelium and produces antibiotic compounds.

antibiotic compound production spore-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 126008

LMG 19357,ATCC 27456,CCRC 12076,DSM 40530,IFO 13433,IMET 43357,JCM 4288,JCM 4809,NCIMB 9445,NRRL 2939,DSM 41250,NCIB 9445,NRRL B-2939,VKM Ac-1911,CBS 461.69,AS 4.1443,KCTC 9165,NBRC 13433,BCRC 12076,CGMCC 4.1443,VTT E-073029

@ref 20215

Last LPSN update 2026-02-09 11:21:42

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces pactus

Full scientific name Streptomyces pactus corrig. Bhuyan et al. 1962 (Approved Lists 1980)

Synonyms (1)

Streptomyces pactum

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	positive	90.635
125439	positive	97.8

Colony morphology

@ref	Colony color	Incubation period	Medium used
19472	Grey	10-14 days	ISP 2
19472		10-14 days	ISP 3
19472	Beige	10-14 days	ISP 4
19472	Beige	10-14 days	ISP 5
19472		10-14 days	ISP 6
19472	Beige	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19472	Aerial Mycelium	Beige	ISP 2
19472	Aerial Mycelium		ISP 3
19472	Aerial Mycelium	Grey	ISP 4
19472	Aerial Mycelium	Grey	ISP 5
19472			ISP 6
19472	Aerial Mycelium	Grey beige	ISP 7

Multimedia

@ref:	9619
multimedia content:	DSM_40530.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40530.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9619	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19472	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19472	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19472	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19472	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19472	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19472	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9619	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 20.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l NaCl 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
9619	positive	growth	28	mesophilic
19472	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.4

Spore formation

@ref	Spore description	Type of spore	Confidence
19472	Formation of spore chains (retinaculiapetri b), spore surface smooth	spore
125438			90.917
125439			92.2

Compound production

@ref	Compound
9619	pactamycin
9619	piericidins
20216	Pactamycin (Pactacin, NSC52947)
20216	Pieridacin
19472	Actinopyrones
19472	Lonomycin A
19472	Pactamycin
19472	Piericidin A
67770	piericidins

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19472	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19472	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19472	28757 ChEBI	fructose	-
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19472	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19472	29864 ChEBI	mannitol	-
19472	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19472	16634 ChEBI	raffinose	-
19472	26546 ChEBI	rhamnose	-
19472	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19472	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E
67770		pactamycin

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19472	+	+	+	+	-	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9619	1	Risk group (German classification) According to TRBA 466
19472	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score	IMG accession
66792	Streptomyces pactum NBRC 13433	complete			68249	96.15	2563366709
66792	Streptomyces pactum ATCC 27456	complete			68249	92.71	2563366708
66792	ASM1603161v1 assembly for Streptomyces pactum ATCC 27456	contig	GCA_016031615	68249.10	68249	68.53

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces pactum strain NRRL ISP-5530 16S ribosomal RNA gene, partial sequence	EF654095	1479	68249
20218	Streptomyces pactum gene for 16S ribosomal RNA, partial sequence, strain: JCM 4809	D44377	121	68249
20218	Streptomyces pactum NBRC 13433 gene for 16S rRNA, partial sequence	AB184398	1464	68249
20218	Streptomyces pactum gene for 16S ribosomal RNA, partial sequence, strain: NBRC 13433	AB915617	1302	68249

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Streptomyces pactum ATCC 27456
NCBI: GCA_016031615

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	92.20	no
125439	motility	BacteriaNetⓘ	no	85.10	no
125439	gram_stain	BacteriaNetⓘ	positive	97.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces pactum strain ATCC 27456
PATRIC: 68249.10

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.64	no
125438	anaerobic	anaerobicⓘ	no	96.83	no
125438	spore-forming	spore-formingⓘ	yes	90.92	yes
125438	aerobic	aerobicⓘ	yes	85.31	no
125438	thermophilic	thermophileⓘ	no	94.37	yes
125438	flagellated	motile2+ⓘ	no	88.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Modulation of Specialized Metabolite Production in Genetically Engineered Streptomyces pactum.	Ju Z, Zhou W, Alharbi HA, Howell DC, Mahmud T	ACS Chem Biol	10.1021/acschembio.1c00718	2021
	Identification and Biological Activity of NFAT-133 Congeners from Streptomyces pactum.	Zhou W, Posri P, Liu XJ, Ju Z, Lan WJ, Mahmud T	J Nat Prod	10.1021/acs.jnatprod.1c00152	2021
Metabolism	Natural Occurrence of Hybrid Polyketides from Two Distinct Biosynthetic Pathways in Streptomyces pactum.	Zhou W, Posri P, Mahmud T	ACS Chem Biol	10.1021/acschembio.0c00982	2021
Metabolism	Biosynthesis of the Nuclear Factor of Activated T Cells Inhibitor NFAT-133 in Streptomyces pactum.	Zhou W, Posri P, Abugrain ME, Weisberg AJ, Chang JH, Mahmud T	ACS Chem Biol	10.1021/acschembio.0c00775	2020
Pathogenicity	Mutasynthesis of fluorinated pactamycin analogues and their antimalarial activity.	Almabruk KH, Lu W, Li Y, Abugreen M, Kelly JX, Mahmud T	Org Lett	10.1021/ol4004614	2013
Metabolism	Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues.	Ito T, Roongsawang N, Shirasaka N, Lu W, Flatt PM, Kasanah N, Miranda C, Mahmud T	Chembiochem	10.1002/cbic.200900339	2009
Metabolism	Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation.	Kudo F, Kasama Y, Hirayama T, Eguchi T	J Antibiot (Tokyo)	10.1038/ja.2007.63	2007
Enzymology	Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530.	Bockle B, Galunsky B, Muller R	Appl Environ Microbiol	10.1128/aem.61.10.3705-3710.1995	1995
Metabolism	Functional Studies and Revision of the NFAT-133/TM-123 Biosynthetic Pathway in Streptomyces pactum.	Zhou W, Alharbi HA, Hummingbird E, Keatinge-Clay AT, Mahmud T	ACS Chem Biol	10.1021/acschembio.2c00454	2022
Phylogeny	Streptomyces hyderabadensis sp. nov., an actinomycete isolated from soil.	Reddy TVK, Mahmood S, Paris L, Reddy YHK, Wellington EMH, Idris MM	Int J Syst Evol Microbiol	10.1099/ijs.0.020446-0	2010

References

#9619	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40530
#19472	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Streptomyces pactus (VKM Ac-1911, NCIMB 9445, DSM 40530)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_016031615

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media