Streptomyces olivaceiscleroticus (DSM 40595, ATCC 15722, CBS 785.72)

Name: Streptomyces olivaceiscleroticus (DSM 40595, ATCC 15722, CBS 785.72)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15434

Type strain

Culture col. no. DSM 40595 ATCC 15722 CBS 785.72 IMRU 3751 ISP 5595 12 more

NCBI tax ID(s) 68245

Special Collections DSMZ JCM KCTC Wink compendium

History <- E.B. Shirling, ISP <- ATCC <- H.A. Lechevalier, IMRU <- M.J. Thirumalachar KCC A-0045 <-- T. Furumai <-- IMRU 3751.

Links

Streptomyces olivaceiscleroticus DSM 40595 is a spore-forming bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 297087

ATCC 15897,ATCC 15722,IFO 13484,DSM 40595,JCM 3045,JCM 4805,CCRC 11608,NRRL B-2318,CBS 785.72,CBS 296.66,NBRC 13484,LMG 20081,BCRC 11608,KCTC 19945,KCTC 19963,CGMCC 4.1991

@ref 20215

Last LPSN update 2026-02-09 11:21:42

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces olivaceiscleroticus

Full scientific name Streptomyces olivaceiscleroticus Pridham 1970 (Approved Lists 1980)

Synonyms (1)

Chainia olivacea

Morphology

Colony morphology

@ref	Colony color	Incubation period	Medium used
19482	Orange	10-14 days	ISP 2
19482	Orange brown	10-14 days	ISP 3
19482	Orange brown	10-14 days	ISP 4
19482	Yellow	10-14 days	ISP 5
19482	Yellow	10-14 days	ISP 6
19482	Yellow	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19482	Aerial Mycelium	White	ISP 2
19482	Aerial Mycelium	Light grey	ISP 3
19482	Aerial Mycelium	White	ISP 4
19482	Aerial Mycelium	White	ISP 5
19482			ISP 6
19482	Aerial Mycelium	Grey	ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9671	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19482	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19482	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19482	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19482	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19482	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19482	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
9671	positive	growth	28
19482	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Spore formation

@ref	Spore description	Type of spore	Spore formation
19482	Formation of spore chains (spirales)(Sp), spore surface smooth	spore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19482	NaCl	positive	maximum	2.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19482	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19482	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
19482	28757 ChEBI	fructose	-
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19482	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19482	29864 ChEBI	mannitol	-
19482	17268 ChEBI	myo-inositol	+/-
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19482	16634 ChEBI	raffinose	+
19482	26546 ChEBI	rhamnose	+/-
19482	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19482	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19482	+	+	-	-	+	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
9671	soil
67770	Soil	Pimpri	India	IND	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9671	1	Risk group (German classification) According to TRBA 466
19482	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3952122v1 assembly for Streptomyces olivaceiscleroticus JCM 4805	contig	GCA_039521225	68245	62.51

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces olivaceiscleroticus 16S rRNA gene, type strain DSM 40595T	AJ621606	1498	68245
20218	Streptomyces olivaceiscleroticus strain DSM 40595 16S ribosomal RNA gene, partial sequence	AY999813	1422	68245
20218	Streptomyces olivaceiscleroticus gene for 16S rRNA, partial sequence	AB122761	565	68245
20218	Streptomyces olivaceiscleroticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4805	D44375	121	68245
20218	Streptomyces olivaceiscleroticus 16S rRNA gene, type strain LMG 20081	AJ781350	1481	68245
20218	Streptomyces olivaceiscleroticus gene for 16S rRNA, partial sequence, strain: NBRC 13484	AB184436	1480	68245
124043	Streptomyces olivaceiscleroticus strain JCM 4805 16S ribosomal RNA gene, partial sequence.	MT760617	1347	68245

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Catabolism of benzoate and monohydroxylated benzoates by Amycolatopsis and Streptomyces spp.	Grund E, Knorr C, Eichenlaub R.	Appl Environ Microbiol	10.1128/aem.56.5.1459-1464.1990	1990

References

#9671	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40595
#19482	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces olivaceiscleroticus (DSM 40595, ATCC 15722, CBS 785.72)

Name and taxonomic classification

Morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039521225

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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