Streptomyces nogalater (DSM 40546, ATCC 27451, CBS 746.72)

Cell morphology

Multimedia

• @ref:	9631
  multimedia content:	DSM_40546-1.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40546-1.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

• @ref:	9631
  multimedia content:	DSM_40546.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40546.jpg
  caption:	Medium 65 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
9631	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9631	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)		Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

Oxygen tolerance

Compound production

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18606	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
18606	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
18606	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
18606	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
18606	29864 ChEBI	mannitol	+
18606	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
18606	16634 ChEBI	raffinose	-
18606	26546 ChEBI	rhamnose	-
18606	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
18606	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E
67770	44504 ChEBI	nogalamycin

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

API zym

Risk assessment

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID	Score	INSDC accession
66792	Streptomyces nogalater ATCC 27451	complete	2563366707	38314	99.69
66792	Streptomyces nogalater ATCC27451	complete	2582581468	38314	99.52
66792	Streptomyces nogalater ATCC 27451	complete	2563366706	38314	74.76
124043	ASM3953288v1 assembly for Streptomyces nogalater JCM 4799	scaffold		38314	51.38	GCA_039532885
124043	ASM4265610v1 assembly for Streptomyces nogalater CGMCC 4.1442	scaffold		38314		GCA_042656105
124043	ASM4267468v1 assembly for Streptomyces nogalater CGMCC 4.1442	contig		38314		GCA_042674685

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Streptomyces nogalater gene for 16S ribosomal RNA, partial sequence, strain: JCM 4799	D44370	120		38314
9631	Streptomyces nogalater gene for 16S rRNA, partial sequence, strain: NBRC 13445	AB184408	1477		38314
9631	Streptomyces nogalater gene for 16S rRNA	AB045886	1485		38314
9631	Streptomyces nogalater gene for 16S rRNA, partial sequece, strain:TT2-9	AB231805	1446		38314
124043	Streptomyces nogalater strain JCM 4799 16S ribosomal RNA gene, partial sequence.	MT760616	866		38314

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Pathway Engineering of Anthracyclines: Blazing Trails in Natural Product Glycodiversification.	Brown KV, Wandi BN, Metsa-Ketela M, Nybo SE.	J Org Chem	10.1021/acs.joc.0c01863	2020
Metabolism	Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin.	Gullon S, Olano C, Abdelfattah MS, Brana AF, Rohr J, Mendez C, Salas JA.	Appl Environ Microbiol	10.1128/aem.00734-06	2006
Metabolism	Genome mining of biosynthetic and chemotherapeutic gene clusters in Streptomyces bacteria.	Belknap KC, Park CJ, Barth BM, Andam CP.	Sci Rep	10.1038/s41598-020-58904-9	2020
	Identification of a cyclase gene dictating the C-9 stereochemistry of anthracyclines from Streptomyces nogalater.	Torkkell S, Kunnari T, Palmu K, Hakala J, Mantsala P, Ylihonko K.	Antimicrob Agents Chemother	10.1128/aac.44.2.396-399.2000	2000
Metabolism	Development of a Streptomyces venezuelae-based combinatorial biosynthetic system for the production of glycosylated derivatives of doxorubicin and its biosynthetic intermediates.	Han AR, Park JW, Lee MK, Ban YH, Yoo YJ, Kim EJ, Kim E, Kim BG, Sohng JK, Yoon YJ.	Appl Environ Microbiol	10.1128/aem.02527-10	2011
Metabolism	Engineering anthracycline biosynthesis toward angucyclines.	Metsa-Ketela M, Palmu K, Kunnari T, Ylihonko K, Mantsala P.	Antimicrob Agents Chemother	10.1128/aac.47.4.1291-1296.2003	2003
	The Brazilian Caatinga Biome as a Hotspot for the Isolation of Antibiotic-Producing Actinomycetota.	Fernandes SP, de Almeida LLC, de Andrade ASA, Abreu LS, Nascimento YM, de Souza TA, da Silva EF, Volpato FCZ, Barth AL, Tavares JF, de Araujo DAM, Rodrigues-Junior VDS, Cibulski SP.	Life (Basel)	10.3390/life15101494	2025
Metabolism	Mechanism of Uncoupled Carbocyclization and Epimerization Catalyzed by Two Non-Heme Iron/alpha-Ketoglutarate Dependent Enzymes.	Li H, Zhu W, Liu Y.	J Chem Inf Model	10.1021/acs.jcim.9b00837	2019
	The snogI Gene is Necessary for the Proper Functioning of the Nogalamycin Biosynthesis Pathway.	Li M, Zhang Z.	Indian J Microbiol	10.1007/s12088-021-00941-7	2021
Enzymology	Antifungal activity of 3-acetylbenzamide produced by actinomycete WA23-4-4 from the intestinal tract of Periplaneta americana.	Fang X, Shen J, Wang J, Chen ZL, Lin PB, Chen ZY, Liu LY, Zeng HX, Jin XB.	J Microbiol	10.1007/s12275-018-7510-z	2018
Enzymology	[THE ROLE OF (p)ppGpp MOLECULES IN FORMATION OF "STRICT RESPONSE" IN BACTERIA AND BIOSYNTHESIS OF ANTIBIOTICS AND MORPHOLOGICAL DIFFERENTIATION IN ACTINOMYCETES].	Klymyshin D, Stephanyshyn O, Fedorenko V.	Tsitol Genet		2016
Metabolism	Crystal structure of the glycosyltransferase SnogD from the biosynthetic pathway of nogalamycin in Streptomyces nogalater.	Claesson M, Siitonen V, Dobritzsch D, Metsa-Ketela M, Schneider G.	FEBS J	10.1111/j.1742-4658.2012.08711.x	2012
Metabolism	Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil.	Jacob J, Rajendran RU, Priya SH, Purushothaman J, Saraswathy Amma DKBN.	PLoS One	10.1371/journal.pone.0175919	2017
	[Design of Streptomyces nogalater LV65 strains with higher synthesis of nogalamicin using regulatory genes].	Klimishin DA, Rabyk MV, Gren' TP, Nimets' OIa, Gonchar MA, Gromyko AN, Fedorenko VA.	Prikl Biokhim Mikrobiol		2011
Enzymology	Crystal structure of the cofactor-independent monooxygenase SnoaB from Streptomyces nogalater: implications for the reaction mechanism.	Grocholski T, Koskiniemi H, Lindqvist Y, Mantsala P, Niemi J, Schneider G.	Biochemistry	10.1021/bi901985b	2010
	Green Nanotechnology-based Gold Nanomaterials for Hepatic Cancer Therapeutics: A Systematic Review.	Barabadi H, Webster TJ, Vahidi H, Sabori H, Damavandi Kamali K, Jazayeri Shoushtari F, Mahjoub MA, Rashedi M, Mostafavi E, Cruz DM, Hosseini O, Saravana M.	Iran J Pharm Res	10.22037/ijpr.2020.113820.14504	2020
	Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities.	El-Naggar NE, El-Sawah AA, Elmansy MF, Elmessiry OT, El-Saidy ME, El-Sherbeny MK, Sarhan MT, Elhefnawy AA, Dalal SR.	Sci Rep	10.1038/s41598-024-54698-2	2024
	Mycothiol Peroxidase Activity as a Part of the Self-Resistance Mechanisms against the Antitumor Antibiotic Cosmomycin D.	Castillo Arteaga RD, Garrido LM, Pedre B, Helmle I, Gross H, Gust B, Padilla G.	Microbiol Spectr	10.1128/spectrum.00493-22	2022
Metabolism	Crystal structures of SnoaL2 and AclR: two putative hydroxylases in the biosynthesis of aromatic polyketide antibiotics.	Beinker P, Lohkamp B, Peltonen T, Niemi J, Mantsala P, Schneider G.	J Mol Biol	10.1016/j.jmb.2006.03.060	2006
	The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures.	Torkkell S, Kunnari T, Palmu K, Mantsala P, Hakala J, Ylihonko K.	Mol Genet Genomics	10.1007/s004380100554	2001
Metabolism	Streptomyces sp. VN1, a producer of diverse metabolites including non-natural furan-type anticancer compound.	Nguyen HT, Pokhrel AR, Nguyen CT, Pham VTT, Dhakal D, Lim HN, Jung HJ, Kim TS, Yamaguchi T, Sohng JK.	Sci Rep	10.1038/s41598-020-58623-1	2020
Metabolism	How a cofactor-free protein environment lowers the barrier to O2 reactivity.	Machovina MM, Ellis ES, Carney TJ, Brushett FR, DuBois JL.	J Biol Chem	10.1074/jbc.ra118.006144	2019
Metabolism	Production of hybrid anthracycline antibiotics by heterologous expression of Streptomyces nogalater nogalamycin biosynthesis genes.	Ylihonko K, Hakala J, Kunnari T, Mantsala P.	Microbiology (Reading)	10.1099/13500872-142-8-1965	1996
	Hybrid anthracyclines from a genetically engineered Streptomyces galilaeus mutant.	Kunnari TJ, Ylihonko KP, Klika KD, Mantsala PI, Hakala JM.	J Org Chem	10.1021/jo980345o	2000
Metabolism	A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway.	Ylihonko K, Tuikkanen J, Jussila S, Cong L, Mantsala P.	Mol Gen Genet	10.1007/bf02172908	1996
	A BioBricks Metabolic Engineering Platform for the Biosynthesis of Anthracyclinones in Streptomyces coelicolor.	Wang R, Nguyen J, Hecht J, Schwartz N, Brown KV, Ponomareva LV, Niemczura M, van Dissel D, van Wezel GP, Thorson JS, Metsa-Ketela M, Shaaban KA, Nybo SE.	ACS Synth Biol	10.1021/acssynbio.2c00498	2022
	Culturable rare actinomycetes from Indian forest soils: Molecular and physicochemical screening for biosynthetic genes.	Bundale S, Singh J, Begde D, Nashikkar N, Upadhyay A.	Iran J Microbiol		2018
	Mining of efficient microbial UDP-glycosyltransferases by motif evolution cross plant kingdom for application in biosynthesis of salidroside.	Fan B, Chen T, Zhang S, Wu B, He B.	Sci Rep	10.1038/s41598-017-00568-z	2017
Metabolism	Expression, purification and crystallization of the cofactor-independent monooxygenase SnoaB from the nogalamycin biosynthetic pathway.	Koskiniemi H, Grocholski T, Schneider G, Niemi J.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s1744309109001389	2009
Enzymology	Monooxygenase Substrates Mimic Flavin to Catalyze Cofactorless Oxygenations.	Machovina MM, Usselman RJ, DuBois JL.	J Biol Chem	10.1074/jbc.m116.730051	2016
Metabolism	Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium.	Giardina A, Alduina R, Gottardi E, Di Caro V, Sussmuth RD, Puglia AM.	Microb Cell Fact	10.1186/1475-2859-9-44	2010
Metabolism	Two novel classes of enzymes are required for the biosynthesis of aurofusarin in Fusarium graminearum.	Frandsen RJ, Schutt C, Lund BW, Staerk D, Nielsen J, Olsson S, Giese H.	J Biol Chem	10.1074/jbc.m110.179853	2011
Metabolism	Structure of a conserved hypothetical protein, TTHA0849 from Thermus thermophilus HB8, at 2.4 A resolution: a putative member of the StAR-related lipid-transfer (START) domain superfamily.	Nakabayashi M, Shibata N, Komori H, Ueda Y, Iino H, Ebihara A, Kuramitsu S, Higuchi Y.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s1744309105035372	2005
	Systematic Search for Evidence of Interdomain Horizontal Gene Transfer from Prokaryotes to Oomycete Lineages.	McCarthy CG, Fitzpatrick DA.	mSphere	10.1128/msphere.00195-16	2016
Metabolism	The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710.	Gao Q, Thorson JS.	FEMS Microbiol Lett	10.1111/j.1574-6968.2008.01112.x	2008
Metabolism	Mammalian and microbial cell-free conversion of anthracycline antibiotics and analogs.	Rueckert PW, Wiley PF, McGovren JP, Marshall VP.	J Antibiot (Tokyo)	10.7164/antibiotics.32.141	1979
Metabolism	Salmochelins, siderophores of Salmonella enterica and uropathogenic Escherichia coli strains, are recognized by the outer membrane receptor IroN.	Hantke K, Nicholson G, Rabsch W, Winkelmann G.	Proc Natl Acad Sci U S A	10.1073/pnas.0737682100	2003
Metabolism	Bacterial metabolism of anthracycline antibiotics. Steffimycinone and steffimycinol conversions.	Wiley PF, Koert JM, Elrod DW, Reisender EA, Marshall VP.	J Antibiot (Tokyo)	10.7164/antibiotics.30.649	1977
Metabolism	Environmental Sensing in Actinobacteria: a Comprehensive Survey on the Signaling Capacity of This Phylum.	Huang X, Pinto D, Fritz G, Mascher T.	J Bacteriol	10.1128/jb.00176-15	2015
Metabolism	Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus.	Chung JY, Fujii I, Harada S, Sankawa U, Ebizuka Y.	J Bacteriol	10.1128/jb.184.22.6115-6122.2002	2002
Enzymology	Functional analysis of OleY L-oleandrosyl 3-O-methyltransferase of the oleandomycin biosynthetic pathway in Streptomyces antibioticus.	Rodriguez L, Rodriguez D, Olano C, Brana AF, Mendez C, Salas JA.	J Bacteriol	10.1128/jb.183.18.5358-5363.2001	2001
Metabolism	Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation.	Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mantsala P, Schneider G.	EMBO J	10.1038/sj.emboj.7600201	2004
	The mithramycin gene cluster of Streptomyces argillaceus contains a positive regulatory gene and two repeated DNA sequences that are located at both ends of the cluster.	Lombo F, Brana AF, Mendez C, Salas JA.	J Bacteriol	10.1128/jb.181.2.642-647.1999	1999
Metabolism	Proteins induced during adaptation of Acetobacter aceti to high acetate concentrations.	Steiner P, Sauer U.	Appl Environ Microbiol	10.1128/aem.67.12.5474-5481.2001	2001
Metabolism	Biosynthetic investigations of lactonamycin and lactonamycin z: cloning of the biosynthetic gene clusters and discovery of an unusual starter unit.	Zhang X, Alemany LB, Fiedler HP, Goodfellow M, Parry RJ.	Antimicrob Agents Chemother	10.1128/aac.00717-07	2008
Metabolism	Biosynthetic gene cluster of simocyclinone, a natural multihybrid antibiotic.	Trefzer A, Pelzer S, Schimana J, Stockert S, Bihlmaier C, Fiedler HP, Welzel K, Vente A, Bechthold A.	Antimicrob Agents Chemother	10.1128/aac.46.5.1174-1182.2002	2002
	Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus.	Aguirrezabalaga I, Olano C, Allende N, Rodriguez L, Brana AF, Mendez C, Salas JA.	Antimicrob Agents Chemother	10.1128/aac.44.5.1266-1275.2000	2000
	Characterization of a Brucella species 25-kilobase DNA fragment deleted from Brucella abortus reveals a large gene cluster related to the synthesis of a polysaccharide.	Vizcaino N, Cloeckaert A, Zygmunt MS, Fernandez-Lago L.	Infect Immun	10.1128/iai.69.11.6738-6748.2001	2001
Metabolism	Characterization of Streptomyces nogalater genes encoding enzymes involved in glycosylation steps in nogalamycin biosynthesis.	Torkkell S, Ylihonko K, Hakala J, Skurnik M, Mantsala P	Mol Gen Genet	10.1007/s004380050562	1997
Cultivation	Spontaneous and induced variability in Streptomyces nogalater producing nogalamycin.	Klanova K, Blumauerova M, Vanek Z	Folia Microbiol (Praha)	10.1007/BF02877658	1977
Phylogeny	Streptomyces roietensis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of jasmine rice, Oryza sativa KDML 105.	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijsem.0.002402	2017
Phylogeny	Streptomyces canchipurensis sp. nov., isolated from a limestone habitat.	Li WJ, Nimaichand S, Jiang Z, Liu MJ, Khieu TN, Kim CJ, Hozzein WN, Park DJ, Wadaan MA, Ningthoujam DS	Antonie Van Leeuwenhoek	10.1007/s10482-014-0281-6	2014