Name: Streptomyces nitrosporeus 0-20
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 15425

Type strain:

Species: Streptomyces nitrosporeus

Strain Designation: 0-20

Culture col. no.: DSM 40023, ATCC 12769, ATCC 19792, CBS 544.68, DSM 41158, ETH 24344, ETH 24415, HUT 6032, IFO 12803, IFO 3362, IMRU 3728, ISP 5023, JCM 4064, KCC S-0064, KCC S-0598, KCTC 9761, NBRC 12803, NBRC 3362, RIA 1071, PSA 206, BCRC 13645, CGMCC 4.1973, JCM 4598, NCIMB 9717, NRRL B-1316, RIA 503, VKM Ac-1191, VKM Ac-1202

Strain history: KCC S-0064 <-- Y. Okami O-20 (NIHJ 21).

NCBI tax ID(s): 28894 (species)

SI-ID 35657

ATCC 12769, ATCC 19792, IFO 3362, IFO 12803, DSM 40023, DSM 41158, JCM 4064, CCRC 13645, HUT 6032, NCIMB 9717, NRRL B-1316, VKM Ac-1191, VKM Ac-1202, JCM 4598, CBS 544.68, KCTC 9761, NBRC 12803, NBRC 3362, BCRC 13645, NCIM 2958, NCIB 9717, CGMCC 4.1973, IMET 43842

Other strains from Streptomyces nitrosporeus

S. nitrosporeus ST031087(HKI),
S. nitrosporeus ST031159(HKI),
S. nitrosporeus ST030496(HKI),

Section

Streptomyces nitrosporeus 0-20 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from garden soil.

Gram-positive
spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces nitrosporeus**
	Full Scientific Name (LPSN)	Streptomyces nitrosporeus Okami 1952 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #19388]	Colony color	Ivory
[Ref.: #19388]	Incubation period	10-14 days
[Ref.: #19388]	Cultivation medium used	ISP 2

[Ref.: #19388]	Colony color	Ivory
[Ref.: #19388]	Incubation period	10-14 days
[Ref.: #19388]	Cultivation medium used	ISP 3

[Ref.: #19388]	Colony color	Ivory
[Ref.: #19388]	Incubation period	10-14 days
[Ref.: #19388]	Cultivation medium used	ISP 4

[Ref.: #19388]	Incubation period	10-14 days
[Ref.: #19388]	Cultivation medium used	ISP 5

[Ref.: #19388]	Incubation period	10-14 days
[Ref.: #19388]	Cultivation medium used	ISP 6

[Ref.: #19388]	Incubation period	10-14 days
[Ref.: #19388]	Cultivation medium used	ISP 7

[Ref.: #19388]	Multicellular complex forming ability	yes
[Ref.: #19388]	Multicellular complex name	Aerial Mycelium
[Ref.: #19388]	Multicellular complex color	Light grey
[Ref.: #19388]	Medium Name (multicellularity)	ISP 2

[Ref.: #19388]	Multicellular complex forming ability	yes
[Ref.: #19388]	Multicellular complex name	Aerial Mycelium
[Ref.: #19388]	Multicellular complex color	Light grey
[Ref.: #19388]	Medium Name (multicellularity)	ISP 3

[Ref.: #19388]	Multicellular complex forming ability	yes
[Ref.: #19388]	Multicellular complex name	Aerial Mycelium
[Ref.: #19388]	Multicellular complex color	Light grey
[Ref.: #19388]	Medium Name (multicellularity)	ISP 4

[Ref.: #19388]	Multicellular complex forming ability	yes
[Ref.: #19388]	Multicellular complex name	Aerial Mycelium
[Ref.: #19388]	Multicellular complex color	Light grey
[Ref.: #19388]	Medium Name (multicellularity)	ISP 5

[Ref.: #19388]	Multicellular complex forming ability	yes
[Ref.: #19388]	Multicellular complex name	Aerial Mycelium
[Ref.: #19388]	Multicellular complex color	Light grey
[Ref.: #19388]	Medium Name (multicellularity)	ISP 6

[Ref.: #19388]	Multicellular complex forming ability	yes
[Ref.: #19388]	Multicellular complex name	Aerial Mycelium
[Ref.: #19388]	Multicellular complex color	Grey
[Ref.: #19388]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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[Ref.: #19388]

Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 7

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Culture medium growth

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Culture medium composition

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Culture medium

STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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		Temperature
[Ref.: #9223]	growth	28 °C
[Ref.: #19388]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #9223]	Temperature range	mesophilic
[Ref.: #19388]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #19388]	Spore description	Formation of spore chains: rectiflixibilis, spore surface: smooth
[Ref.: #19388]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #9223]	Name of produced compound	nitrosporin
[Ref.: #20216]	Name of produced compound	Nitrosporin
[Ref.: #67770]	Name of produced compound	Nitrosporin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19388]		NaCl		maximum	2.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #19388]	arabinose ChEBI	+/-
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19388]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #19388]	fructose ChEBI	-
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19388]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #19388]	mannitol ChEBI	-
[Ref.: #19388]	myo-inositol ChEBI	-
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #19388]	raffinose ChEBI	-
[Ref.: #19388]	rhamnose ChEBI	-
[Ref.: #19388]	sucrose ChEBI	-
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19388]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19388]	254	+	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9223]	Sample type/isolated from	garden soil
[Ref.: #9223]	Country	Japan
[Ref.: #9223]	Country ISO 3 Code	JPN
[Ref.: #9223]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Humus soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Tokyo
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JQ924413 (>99% sequence identity) for Streptomyces from Microbeatlas

Aquatic Samples	286
Soil Samples	2441
Animal Samples	1009
Plant Samples	294
Total Samples	4030

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #9223]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19388]	Biosafety level	1	Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Streptomyces nitrosporeus strain CGMCC 4.1973 clone 1 16S ribosomal RNA gene, complete sequence	JQ924411	1478	ENA	28894 tax ID	*
[Ref.: #20218]	Streptomyces nitrosporeus strain CGMCC 4.1973 clone 3 16S ribosomal RNA gene, complete sequence	JQ924412	1478	ENA	28894 tax ID	*
[Ref.: #20218]	Streptomyces nitrosporeus strain CGMCC 4.1973 clone 4 16S ribosomal RNA gene, complete sequence	JQ924413	1478	ENA	28894 tax ID	*
[Ref.: #20218]	Streptomyces nitrosporeus gene for 16S rRNA, partial sequence	AB122747	564	ENA	28894 tax ID	*
[Ref.: #20218]	Streptomyces nitrosporeus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4064	D43996	120	ENA	28894 tax ID	*
[Ref.: #20218]	Streptomyces nitrosporeus gene for 16S rRNA, partial sequence, strain: NBRC 12803	AB184159	1468	ENA	28894 tax ID	*
[Ref.: #20218]	Streptomyces nitrosporeus gene for 16S rRNA, partial sequence, strain: NBRC 3362	AB184751	1475	ENA	28894 tax ID	*
[Ref.: #20218]	Streptomyces nitrosporeus strain NRRL B-1316 16S ribosomal RNA gene, partial sequence	EF178680	1487	ENA	28894 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptomyces nitrosporeus ATCC 12769	GCA_008704555	complete	GenBank	28894 tax ID	*
[Ref.: #66792]	Streptomyces nitrosporeus JCM 4598	GCA_014650375	scaffold	GenBank	28894 tax ID	*
[Ref.: #66792]	Streptomyces nitrosporeus strain ATCC 12769	28894.3	complete	PATRIC	28894 tax ID	*
[Ref.: #66792]	Streptomyces nitrosporeus strain JCM 4598	28894.4	wgs	PATRIC	28894 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptomyces nitrosporeus ATCC 12769 NCBI: GCA_008704555.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptomyces nitrosporeus ATCC 12769 NCBI: GCA_008704555.1
[Ref.: #69480]	motile	no	94.661	no
[Ref.: #69480]	flagellated	no	98.194	no
[Ref.: #69480]	gram-positive	yes	92.03	no
[Ref.: #69480]	anaerobic	no	99.168	no
[Ref.: #69480]	aerobic	yes	93.925	no
[Ref.: #69480]	halophile	no	93.826	no
[Ref.: #69480]	spore-forming	yes	95.17	yes
[Ref.: #69480]	thermophile	no	98.252	yes
[Ref.: #69480]	glucose-util	yes	88.714	yes
[Ref.: #69480]	glucose-ferment	no	87.621	no

Availability in culture collections External links

[Ref.: #9223]

Culture collection no.

DSM 40023, ATCC 12769, ATCC 19792, CBS 544.68, DSM 41158, ETH 24344, ETH 24415, HUT 6032, IFO 12803, IFO 3362, IMRU 3728, ISP 5023, JCM 4064, KCC S-0064, KCC S-0598, KCTC 9761, NBRC 12803, NBRC 3362, RIA 1071, PSA 206, BCRC 13645, CGMCC 4.1973, JCM 4598, NCIMB 9717, NRRL B-1316, RIA 503, VKM Ac-1191, VKM Ac-1202

[Ref.: #84512]

SI-ID 35657

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces maoxianensis sp. nov., a novel actinomycete isolated from soil in Maoxian, China.	Guan X, Liu C, Zhao J, Fang B, Zhang Y, Li L, Jin P, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-015-0403-9	2015	*

References

#9223

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40023

#19388

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#84512

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35657.1 )

* These data were automatically processed and therefore are not curated

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Streptomyces nitrosporeus ATCC 12769

Streptomyces nitrosporeus ATCC 12769

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