Streptomyces intermedius (DSM 40372, ATCC 3329, CBS 101.21)

Name: Streptomyces intermedius (DSM 40372, ATCC 3329, CBS 101.21)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15329

Type strain

Strain Designation Wollenweber

Culture col. no. DSM 40372 ATCC 3329 CBS 101.21 CBS 694.69 IFO 13049 16 more

NCBI tax ID(s) 53449

Special Collections DSMZ JCM Wink compendium JMRC

History DSMZ <- E.B. Shirling, ISP <- CBS JCM <-- KCC S-0197 <-- NRRL B-1327.

Links

Streptomyces intermedius Wollenweber is a spore-forming bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 92626

LMG 19304,ATCC 25461,ATCC 3329,CCRC 13706,DSM 40372,IFO 13049,IMET 41384,JCM 4197,JCM 4483,NRRL B-1327,NRRL B-2670,CBS 101.21,CBS 694.69,AS 4.1467,NBRC 13049,BCRC 13706,ICMP 12540,CGMCC 4.1467

@ref 20215

Last LPSN update 2026-05-29 08:28:30

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces intermedius

Full scientific name Streptomyces intermedius (Krüger 1904) Waksman 1953 (Approved Lists 1980)

Synonyms (1)

"Oospora intermedia"

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125439		93.397
125439		99.176	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
19447	Yellowish-brown	10-14 days	ISP 2
19447	Yellow	10-14 days	ISP 3
19447	Yellow	10-14 days	ISP 4
19447	Yellow	10-14 days	ISP 5

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19447	Aerial Mycelium	White	ISP 2
19447	Aerial Mycelium	Ivory	ISP 3
19447	Aerial Mycelium	Ivory	ISP 4
19447	Aerial Mycelium	White	ISP 5

Multimedia

@ref:	9288
multimedia content:	DSM_40372.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40372.jpg
caption:	Medium 84 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9288	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19447	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19447	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19447	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19447	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
9288	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
9288	positive	growth	28
19447	positive	optimum	30
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	facultative anaerobe	98.802

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
19447	Formation of spore chains: rectiflixibilis, spore surface: smooth	spore
125439				98.75

Compound production

20216

CompoundHeptaenic antifungal activity

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19447	NaCl	positive	maximum	2.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19447	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
19447	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
19447	28757 ChEBI	fructose	+/-
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19447	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19447	29864 ChEBI	mannitol	+
19447	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19447	16634 ChEBI	raffinose	-
19447	26546 ChEBI	rhamnose	-
19447	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
19447	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19447	-	-	-	-	+	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

9288

Sample typesoil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9288	1	Risk group (German classification) According to TRBA 466
19447	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3953278v1 assembly for Streptomyces intermedius JCM 4483	scaffold	GCA_039532785	53449	17

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	S.intermedius (DSM 40372T) 16S rRNA gene	Z76686	1474	53449
20218	Streptomyces intermedius gene for 16S ribosomal RNA, partial sequence, strain: JCM 4483	D44178	120	53449
20218	Streptomyces intermedius gene for 16S rRNA, partial sequence, strain: NBRC 13049	AB184277	1475	53449
20218	Streptomyces intermedius 16S ribosomal RNA gene, partial sequence	KC355423	817	53449

GC content

@ref	GC-content (mol%)	Method
67770	71	fluorimetric

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM3953278v1 assembly for Streptomyces intermedius JCM 4483
NCBI: GCA_039532785

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	98.80	no
125439	gram_stain	BacteriaNetⓘ	positive	99.18	no
125439	motility	BacteriaNetⓘ	no	93.40	no
125439	spore_formation	BacteriaNetⓘ	yes	98.75	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Screening and characterization of soil bacteria for lignin and textile dye effluent bioremediation and optimization using response surface methodology.	Milad S, Saleh SE, Aboulwafa MM, Hassouna NA.	Sci Rep	10.1038/s41598-025-04789-5	2025
Phylogeny	Comprehensive investigation of marine Actinobacteria associated with the sponge Halichondria panicea.	Schneemann I, Nagel K, Kajahn I, Labes A, Wiese J, Imhoff JF.	Appl Environ Microbiol	10.1128/aem.00780-10	2010
	Unveiling the bioactive potential of Actinomycetota from the Tagus River estuary.	Dos Santos JDN, Pinto E, Martin J, Vicente F, Reyes F, Lage OM.	Int Microbiol	10.1007/s10123-024-00483-0	2024
Phylogeny	Streptomyces fuscigenes sp. nov., isolated from bamboo (Sasa borealis) litter.	Lee HJ, Whang KS	Int J Syst Evol Microbiol	10.1099/ijsem.0.002706	2018
Phylogeny	Streptomyces alfalfae sp. nov. and comparisons with its closest taxa Streptomyces silaceus, Streptomyces flavofungini and Streptomyces intermedius.	She W, Sun Z, Yi L, Zhao S, Liang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.000671	2015
Phylogeny	Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger).	Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R, Zhang Y, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-013-0021-3	2013

References

#9288	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40372
#19447	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces intermedius (DSM 40372, ATCC 3329, CBS 101.21)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039532785

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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