Streptomyces hygroscopicus M5-13184 | Type strain | DSM 40578, ATCC 27438, CBS 773.72, DSM 41148, ETH 28553, IFO 13472, ISP 5578, JCM 4772, NBRC 13472, NRRL 2387, RIA 1433, BCRC 11611, CGMCC 4.1313, CGMCC 4.1527, CGMCC 4.2118, CIP 106840, KACC 20099, LMG 19335, MTCC 1105, NBIMCC 1154, NBIMCC 803, VKM Ac-831

Caption:

Medium 65 28°C

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Information on culture and growth conditions Culture and growth conditions

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

expand / minimize

Culture medium

ISP 2 agar

Culture medium growth

Culture medium

ISP 3 agar

Culture medium growth

Culture medium

ISP 4 agar

Culture medium growth

Culture medium

ISP 5 agar

Culture medium growth

[Ref.: #35999]

Culture medium

MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicum

[Ref.: #35999]

Culture medium growth

[Ref.: #35999]

Culture medium composition

expand / minimize

Culture medium

CIP Medium 116

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

	Kind of temperature		Temperature
[Ref.: #9659]		growth	28 °C
[Ref.: #25185]		growth	15.0-45.0 °C
[Ref.: #35999]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #120019]		growth	25-41 °C
[Ref.: #120019]	no	growth	10 °C
[Ref.: #120019]	no	growth	45 °C

[Ref.: #9659]	Temperature range	mesophilic
[Ref.: #35999]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120019]	Temperature range	psychrophilic
[Ref.: #120019]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

obligate aerobe

[Ref.: #25185]	Type of spore	spore
[Ref.: #25185]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

Only first 5 entries are displayed. Click here to see all.

Name of produced compound

hygromycin

	Salt	Tested relation		Salt conc.
[Ref.: #120019]	NaCl		growth	0-4	%
[Ref.: #120019]	NaCl	no	growth	6	%
[Ref.: #120019]	NaCl	no	growth	8	%
[Ref.: #120019]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25185]	adenine ChEBI	+	degradation
[Ref.: #25185]	cellobiose ChEBI	+	assimilation
[Ref.: #25185]	D-fructose ChEBI	+	assimilation
[Ref.: #25185]	D-fucose ChEBI	+	assimilation
[Ref.: #25185]	D-galactose ChEBI	+	assimilation
[Ref.: #25185]	D-glucose ChEBI	+	assimilation
[Ref.: #25185]	D-mannose ChEBI	+	assimilation
[Ref.: #25185]	D-ribose ChEBI	+	assimilation
[Ref.: #25185]	D-sorbitol ChEBI	-	assimilation
[Ref.: #25185]	D-xylose ChEBI	+	assimilation
[Ref.: #120019]	esculin ChEBI	+	hydrolysis
[Ref.: #25185]	guanine ChEBI	+	degradation
[Ref.: #120019]	hippurate ChEBI	-	hydrolysis
[Ref.: #25185]	hypoxanthine ChEBI	+	degradation
[Ref.: #25185]	L-arabinose ChEBI	+	assimilation
[Ref.: #25185]	L-rhamnose ChEBI	+	assimilation
[Ref.: #25185]	lactose ChEBI	+	assimilation
[Ref.: #25185]	maltose ChEBI	+	assimilation
[Ref.: #25185]	mannitol ChEBI	+	assimilation
[Ref.: #25185]	melezitose ChEBI	-	assimilation
[Ref.: #25185]	nitrate ChEBI	-	reduction
[Ref.: #120019]	nitrate ChEBI	-	reduction
[Ref.: #120019]	nitrate ChEBI	-	respiration
[Ref.: #120019]	nitrite ChEBI	-	reduction
[Ref.: #25185]	raffinose ChEBI	-	assimilation
[Ref.: #25185]	sucrose ChEBI	+	assimilation
[Ref.: #25185]	tween 20 ChEBI	+	degradation
[Ref.: #25185]	tween 80 ChEBI	+	degradation
[Ref.: #25185]	xanthine ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120019]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #67770]		hygromycin ChEBI	yes
[Ref.: #120019]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #120019]		acetoin ChEBI	-
[Ref.: #120019]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #120019]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #120019]	amylase	-
[Ref.: #120019]	beta-galactosidase	+	3.2.1.23
[Ref.: #120019]	caseinase	+	3.4.21.50
[Ref.: #120019]	catalase	+	1.11.1.6
[Ref.: #120019]	DNase	+
[Ref.: #120019]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #120019]	gelatinase	+/-
[Ref.: #120019]	lecithinase	-
[Ref.: #120019]	lipase	-
[Ref.: #120019]	lysine decarboxylase	-	4.1.1.18
[Ref.: #120019]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120019]	oxidase	-
[Ref.: #120019]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #120019]	protease	+
[Ref.: #120019]	tryptophan deaminase	-
[Ref.: #120019]	tween esterase	+
[Ref.: #120019]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120019]	1869	not determinedn.d.	+	+	+	+	+	-	-	-	-	+	+	+	+	-	-	+	+	+	+	+	+	-	+	-	-	-	+	-	-	-	-	+	-	+	+	-	-	+	-	-	+	+	-	+	+	-	-	+	+	+	+	-	-	+	+	+	-	+	-	+	+	+	+	-	-	-	-	-	-	-	+	-	+	+	-	+	-	-	-	+	+	-	-	-	-	-	-	+	+	+	+	+	-	+	+	-	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence HQ244447 (>99% sequence identity) for Streptomyces from Microbeatlas

Aquatic Samples	65
Soil Samples	635
Animal Samples	94
Plant Samples	40
Total Samples	834

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #9659]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120019]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #9659]	Streptomyces hygroscopicus subsp. hygroscopicus gene for 16S rRNA, partial sequence, strain: NBRC 13472	AB184428	1470	ENA	68042 tax ID
[Ref.: #20218]	Streptomyces hygroscopicus subsp. hygroscopicus strain CGMCC 4.1527 16S ribosomal RNA gene, partial sequence	HQ244447	1401	ENA	68042 tax ID	*
[Ref.: #20218]	Streptomyces hygroscopicus subsp. hygroscopicus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4772	D44349	121	ENA	68042 tax ID	*
[Ref.: #20218]	Streptomyces hygroscopicus subsp. hygroscopicus gene for 16S rRNA, partial sequece, strain: PNK1-3	AB231803	1450	ENA	68042 tax ID	*
[Ref.: #20218]	Streptomyces hygroscopicus subsp. hygroscopicus gene for 16S rRNA, partial sequece, strain: PNK1-5	AB231804	1451	ENA	68042 tax ID	*
[Ref.: #20218]	Streptomyces hygroscopicus partial 16S rRNA gene, type strain NRRL 2387T	AJ391820	1465	ENA	1912 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Streptomyces hygroscopicus subsp. hygroscopicus NBRC 13472	GCA_001553455	contig	GenBank	68042 tax ID
[Ref.: #66792]	Streptomyces hygroscopicus subsp. hygroscopicus strain NBRC 13472	68042.6	wgs	PATRIC	68042 tax ID	*
[Ref.: #66792]	Streptomyces hygroscopicus hygroscopicus NBRC 13472	2724679234	draft	JGI IMG/M	68042 tax ID	*
[Ref.: #66792]	Streptomyces hygroscopicus hygroscopicus DSM 40578	651716764	draft	JGI IMG/M	68042 tax ID	*

[Ref.: #25185]	GC-content	72 mol%	genome sequence analysis
[Ref.: #9659]	GC-content	71.97 mol%	sequence analysis
[Ref.: #67770]	GC-content	71.97 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	71.9 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptomyces hygroscopicus subsp. hygroscopicus strain NBRC 13472 PATRIC: 68042.6
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptomyces hygroscopicus subsp. hygroscopicus strain NBRC 13472 PATRIC: 68042.6
[Ref.: #69480]	gram-positive	yes	88.854	no
[Ref.: #69480]	anaerobic	no	99.109	no
[Ref.: #69480]	halophile	no	90.249	no
[Ref.: #69480]	spore-forming	yes	95.715	yes
[Ref.: #69480]	glucose-util	yes	88.974	yes
[Ref.: #69480]	flagellated	no	97.898	no
[Ref.: #69480]	thermophile	no	96.985	no
[Ref.: #69480]	aerobic	yes	93.35	no
[Ref.: #69480]	glucose-ferment	no	90.739	no
[Ref.: #69480]	motile	no	93.825	no

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