Streptomyces griseoincarnatus (DSM 40274, ATCC 23623, ATCC 23917)

Name: Streptomyces griseoincarnatus (DSM 40274, ATCC 23623, ATCC 23917)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15267

Type strain

Culture col. no. DSM 40274 ATCC 23623 ATCC 23917 CBS 838.68 IFO 12871 12 more

NCBI tax ID(s) 29305

Special Collections DSMZ CCUG JCM KCTC Wink compendium

Links

Streptomyces griseoincarnatus DSM 40274 is a mesophilic prokaryote that builds an aerial mycelium and was isolated from soil.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 14104

LMG 19316,ATCC 23623,ATCC 23917,CCRC 11481,DSM 40274,IFO 12871,JCM 4381,NCIMB 9825,NRRL B-5313,AS 4.1409,NBRC 12871,BCRC 11481,KCTC 19071,NCIB 9825,CGMCC 4.1409,VTT E-022184,CBS 838.68

@ref 20215

Last LPSN update 2025-12-16 09:50:33

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces griseoincarnatus

Full scientific name Streptomyces griseoincarnatus (Preobrazhenskaya et al. 1957) Pridham et al. 1958 (Approved Lists 1980)

Synonyms (4)

"Actinomyces variabilis"
"Actinomyces griseoincarnatus"
Streptomyces variabilis
Streptomyces erythrogriseus

BacDive ID	Other strains from **Streptomyces griseoincarnatus** (2)	Type strain
128387	S. griseoincarnatus STI10664(IMET), SN 588
128388	S. griseoincarnatus

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99.8
125438	negative	99.75

Colony morphology

@ref	Colony color	Incubation period	Medium used
19430	Black red	10-14 days	ISP 2
19430	Black red	10-14 days	ISP 3
19430	Black red	10-14 days	ISP 4
19430	Black red	10-14 days	ISP 5
19430	Black red	10-14 days	ISP 6
19430	Black red	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19430	Aerial Mycelium	Concrete grey	ISP 2
19430	Aerial Mycelium	Cocrete grey	ISP 3
19430	Aerial Mycelium	Concrete grey	ISP 4
19430	Aerial Mycelium	Ivory	ISP 5
19430			ISP 6
19430	Aerial Mycelium		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9425	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19430	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19430	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19430	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19430	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19430	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19430	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
9425	positive	growth	28	mesophilic
19430	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	97.6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
19430	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	from API 20E
19430	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	from API 20E
19430	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	from API 20E
19430	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	from API 20E
19430	29864 ChEBI	mannitol	+
19430	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	+	from API 20E
19430	16634 ChEBI	raffinose	+
19430	26546 ChEBI	rhamnose	+
19430	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	from API 20E
68368	16199 ChEBI	urea	+	from API 20E
19430	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68368	15688 ChEBI	acetoin	+	from API 20E
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19430	+	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19430	not determinedn.d.	+	+	+	-	+	+	-	+	-	+	-	+	+	-	+	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Continent
9425	soil	USSR	Asia
67770	Soil

Taxonmaps

69479

Global distribution of 16S sequence AJ781321 (>99% sequence identity) for Streptomyces from Microbeatlas

Aquatic Samples
Soil Samples	30
Animal Samples	18
Plant Samples	2
Total Samples	50

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9425	1	Risk group (German classification) According to TRBA 466
19430	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM1464957v1 assembly for Streptomyces griseoincarnatus JCM 4381	scaffold	GCA_014649575	29305.3	8109853046	29305	29.95

Genome browser: GCA_014649575

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces griseoincarnatus strain JCM 4381 16S ribosomal RNA gene, partial sequence	AY999749	1413	29305
20218	Streptomyces griseoincarnatus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4381	D44096	121	29305
20218	Streptomyces griseoincarnatus 16S rRNA gene, type strain LMG 19316	AJ781321	1480	29305
20218	Streptomyces griseoincarnatus gene for 16S rRNA, partial sequence, strain: NBRC 12871	AB184207	1479	29305
124043	Streptomyces griseoincarnatus strain JCM 4381 16S ribosomal RNA gene, partial sequence.	MT760527	1350	29305
124043	Streptomyces griseoincarnatus strain LMG 19316(T) 16S ribosomal RNA gene, partial sequence.	MN687852	523	29305

GC content

@ref	GC-content (mol%)	Method
67770	72.1	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Streptomyces griseoincarnatus JCM 4381
NCBI: GCA_014649575

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	88.80	no
125439	motility	BacteriaNetⓘ	no	88.60	no
125439	gram_stain	BacteriaNetⓘ	positive	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Escherichia coli NCTC9033
NCBI: GCA_900635225.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.75	no
125438	anaerobic	anaerobicⓘ	no	95.96	no
125438	aerobic	aerobicⓘ	no	68.51	no
125438	spore-forming	spore-formingⓘ	no	88.45	no
125438	thermophilic	thermophileⓘ	no	98.50	yes
125438	flagellated	motile2+ⓘ	yes	75.53	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft genome dataset of Streptomyces griseoincarnatus strain R-35 isolated from tidal pool sediments.	Mitchell DD, Vreulink JM, Prins A, Le Roes-Hill M.	Data Brief	10.1016/j.dib.2024.111235	2025
Genetics	Whole genome sequencing of a novel chitinolytic Streptomyces sp. RB7AG reveals it's chitosan production potential: optimization of the process through Taguchi experimental design.	Behera SS, Nivedita S, Das S, Behera HT, Mojumdar A, Ray L.	3 Biotech	10.1007/s13205-023-03613-z	2023
Enzymology	Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China.	Guan TW, Lin YJ, Ou MY, Chen KB.	PLoS One	10.1371/journal.pone.0236006	2020
	Wastewater from the Edible Oil Industry as a Potential Source of Lipase- and Surfactant-Producing Actinobacteria.	Welz P, Swanepoel G, Weels S, Le Roes-Hill M.	Microorganisms	10.3390/microorganisms9091987	2021
Phylogeny	Streptomyces euryhalinus sp. nov., a new actinomycete isolated from a mangrove forest.	Biswas K, Choudhury JD, Mahansaria R, Saha M, Mukherjee J	J Antibiot (Tokyo)	10.1038/ja.2017.3	2017
Phylogeny	Streptomyces tunisiensis sp. nov., a novel Streptomyces species with antibacterial activity.	Slama N, Mankai H, Ayed A, Mezhoud K, Rauch C, Lazim H, Barkallah I, Gtari M, Limam F	Antonie Van Leeuwenhoek	10.1007/s10482-013-0086-z	2013
Phylogeny	Streptomyces wuyuanensis sp. nov., an actinomycete from soil.	Zhang X, Zhang J, Zheng J, Xin D, Xin Y, Pang H	Int J Syst Evol Microbiol	10.1099/ijs.0.047050-0	2013

References

#9425	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40274
#19430	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces griseoincarnatus (DSM 40274, ATCC 23623, ATCC 23917)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014649575

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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