Streptomyces griseochromogenes (DSM 40499, ATCC 14511, CBS 714.72)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	9600
  multimedia content:	DSM_40499.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40499.jpg
  caption:	Medium 84 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
9600	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19467	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19467	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19467	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19467	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19467	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19467	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9600	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)		Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

Oxygen tolerance

Spore formation

Compound production

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19467	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19467	62968 ChEBI	cellulose	+
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19467	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19467	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19467	29864 ChEBI	mannitol	+
19467	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19467	16634 ChEBI	raffinose	+
19467	26546 ChEBI	rhamnose	-
19467	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19467	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
67770		blasticidin A
67770	15353 ChEBI	blasticidin S
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E
67770	134606 ChEBI	toyocamycin

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	biotin biosynthesis	100	4 of 4
66794	1,4-dihydroxy-6-naphthoate biosynthesis	100	6 of 6
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	lipoate biosynthesis	100	5 of 5
66794	glycogen metabolism	100	5 of 5
66794	cardiolipin biosynthesis	100	7 of 7
66794	ethanol fermentation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	adipate degradation	100	2 of 2
66794	IAA biosynthesis	100	3 of 3
66794	starch degradation	100	10 of 10
66794	methylglyoxal degradation	100	5 of 5
66794	butanoate fermentation	100	4 of 4
66794	resorcinol degradation	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	phenylmercury acetate degradation	100	2 of 2
66794	metabolism of amino sugars and derivatives	100	5 of 5
66794	aspartate and asparagine metabolism	100	9 of 9
66794	ppGpp biosynthesis	100	4 of 4
66794	sulfopterin metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	glycine betaine biosynthesis	100	5 of 5
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	cyanate degradation	100	3 of 3
66794	taurine degradation	100	1 of 1
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	aerobactin biosynthesis	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	aminopropanol phosphate biosynthesis	100	2 of 2
66794	cellulose degradation	100	5 of 5
66794	citric acid cycle	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	degradation of aromatic, nitrogen containing compounds	91.67	11 of 12
66794	pentose phosphate pathway	90.91	10 of 11
66794	proline metabolism	90.91	10 of 11
66794	4-hydroxyphenylacetate degradation	90	9 of 10
66794	threonine metabolism	90	9 of 10
66794	propionate fermentation	90	9 of 10
66794	Entner Doudoroff pathway	90	9 of 10
66794	myo-inositol biosynthesis	90	9 of 10
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	allantoin degradation	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	ketogluconate metabolism	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	aclacinomycin biosynthesis	85.71	6 of 7
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	propanol degradation	85.71	6 of 7
66794	glutathione metabolism	85.71	12 of 14
66794	vitamin B12 metabolism	85.29	29 of 34
66794	leucine metabolism	84.62	11 of 13
66794	pyrimidine metabolism	84.44	38 of 45
66794	arginine metabolism	83.33	20 of 24
66794	purine metabolism	82.98	78 of 94
66794	alanine metabolism	82.76	24 of 29
66794	metabolism of disaccharids	81.82	9 of 11
66794	peptidoglycan biosynthesis	80	12 of 15
66794	3-chlorocatechol degradation	80	4 of 5
66794	hydrogen production	80	4 of 5
66794	phenol degradation	80	16 of 20
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	tryptophan metabolism	78.95	30 of 38
66794	heme metabolism	78.57	11 of 14
66794	photosynthesis	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	4-hydroxymandelate degradation	77.78	7 of 9
66794	lipid metabolism	77.42	24 of 31
66794	vitamin B1 metabolism	76.92	10 of 13
66794	urea cycle	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	non-pathway related	76.32	29 of 38
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	toluene degradation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	cyclohexanol degradation	75	3 of 4
66794	3-phenylpropionate degradation	73.33	11 of 15
66794	d-xylose degradation	72.73	8 of 11
66794	degradation of sugar acids	72	18 of 25
66794	oxidative phosphorylation	70.33	64 of 91
66794	sulfate reduction	69.23	9 of 13
66794	isoprenoid biosynthesis	69.23	18 of 26
66794	lysine metabolism	69.05	29 of 42
66794	degradation of sugar alcohols	68.75	11 of 16
66794	androgen and estrogen metabolism	68.75	11 of 16
66794	ascorbate metabolism	68.18	15 of 22
66794	degradation of pentoses	67.86	19 of 28
66794	acetoin degradation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	enterobactin biosynthesis	66.67	2 of 3
66794	nitrate assimilation	66.67	6 of 9
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	histidine metabolism	65.52	19 of 29
66794	methionine metabolism	65.38	17 of 26
66794	tyrosine metabolism	64.29	9 of 14
66794	cholesterol biosynthesis	63.64	7 of 11
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	carnitine metabolism	62.5	5 of 8
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	creatinine degradation	60	3 of 5
66794	glycine metabolism	60	6 of 10
66794	gallate degradation	60	3 of 5
66794	coenzyme M biosynthesis	60	6 of 10
66794	bacilysin biosynthesis	60	3 of 5
66794	arachidonate biosynthesis	60	3 of 5
66794	daunorubicin biosynthesis	55.56	5 of 9
66794	degradation of hexoses	55.56	10 of 18
66794	phenylpropanoid biosynthesis	53.85	7 of 13
66794	carotenoid biosynthesis	50	11 of 22
66794	CMP-KDO biosynthesis	50	2 of 4
66794	kanosamine biosynthesis II	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	vitamin E metabolism	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	ribulose monophosphate pathway	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	alginate biosynthesis	50	2 of 4
66794	grixazone biosynthesis	50	1 of 2
66794	polyamine pathway	47.83	11 of 23
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	vitamin B6 metabolism	45.45	5 of 11
66794	arachidonic acid metabolism	44.44	8 of 18
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	D-cycloserine biosynthesis	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	factor 420 biosynthesis	40	2 of 5
66794	lipid A biosynthesis	33.33	3 of 9
66794	methanogenesis from CO2	33.33	4 of 12
66794	sulfoquinovose degradation	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	methane metabolism	33.33	1 of 3
66794	mevalonate metabolism	28.57	2 of 7
66794	chlorophyll metabolism	27.78	5 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4

API 20E

API zym

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Streptomyces griseochromogenes DSM 40499	complete			2563366698	68214	99.59
66792	ASM154262v2 assembly for Streptomyces griseochromogenes ATCC 14511	complete	GCA_001542625	68214.14	2873104240	68214	98.87
66792	Streptomyces griseochromogenes strain ATCC 14511	contig		68214.3		68214	72.8
66792	ASM1787449v1 assembly for Streptomyces griseochromogenes DSM 40499	contig	GCA_017874495	68214.12	2913531621	68214	61.69

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Streptomyces griseochromogenes partial 16S rRNA gene, type strain DSM 40499	AJ310923	1448		68214
20218	Streptomyces griseochromogenes partial 16S rRNA gene, strain ISP 5499	AJ399491	1449		68214
20218	Streptomyces griseochromogenes gene for 16S ribosomal RNA, partial sequence, strain: JCM 4764	D44343	120		68214
20218	Streptomyces griseochromogenes gene for 16S rRNA, partial sequence, strain: NBRC 13413	AB184387	1474		68214
124043	Streptomyces griseochromogenes strain ATCC 14511(T) 16S ribosomal RNA gene, partial sequence.	MN686613	1413		68214

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Microtiter Plate Cultivation Systems Enable Chemically Diverse Metabolic Footprints During Bacterial Natural Product Discovery.	Lindig A, Hubmann G, Lutz S.	Biotechnol Bioeng	10.1002/bit.29002	2025
	Discovery and Characterization of Oxazolismycin, an Oxazole-Containing Natural Product with an Atypical Loading Mechanism.	Pan Y, Jiang G, Huang C, Zhao H.	JACS Au	10.1021/jacsau.5c00326	2025
Metabolism	A [3Fe-4S] cluster and tRNA-dependent aminoacyltransferase BlsK in the biosynthesis of Blasticidin S.	Wang X, Zhao Y, Gao Y, Luo X, Du A, Deng Z, Zabriskie TM, He X, Jiang M.	Proc Natl Acad Sci U S A	10.1073/pnas.2102318118	2021
Genetics	Pan-Genome of the Genus Streptomyces and Prioritization of Biosynthetic Gene Clusters With Potential to Produce Antibiotic Compounds.	Caicedo-Montoya C, Manzo-Ruiz M, Rios-Estepa R.	Front Microbiol	10.3389/fmicb.2021.677558	2021
Metabolism	Genome mining of biosynthetic and chemotherapeutic gene clusters in Streptomyces bacteria.	Belknap KC, Park CJ, Barth BM, Andam CP.	Sci Rep	10.1038/s41598-020-58904-9	2020
	Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination.	Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin TA, Zhao H.	Nat Commun	10.1038/s41467-021-21275-4	2021
Metabolism	Isolation of beta-1,3-Glucanase-Producing Microorganisms from Poria cocos Cultivation Soil via Molecular Biology.	Wu Q, Dou X, Wang Q, Guan Z, Cai Y, Liao X.	Molecules	10.3390/molecules23071555	2018
Cultivation	Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria.	Schwarz J, Hubmann G, Rosenthal K, Lutz S	Biomolecules	10.3390/biom11020193	2021
Genetics	Complete genome sequence of Streptomyces griseochromogenes ATCC 14511(T), a producer of nucleoside compounds and diverse secondary metabolites.	Wu L, Chen G, Feng G	J Biotechnol	10.1016/j.jbiotec.2017.03.017	2017