Streptomyces diastaticus (DSM 40324, AS 4.1854, ATCC 10975)

Name: Streptomyces diastaticus (DSM 40324, AS 4.1854, ATCC 10975)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15255

Type strain

Strain Designation Duché

Culture col. no. DSM 40324 AS 4.1854 ATCC 10975 ATCC 25455 CBS 422.34 23 more

NCBI tax ID(s) 53448

Special Collections DSMZ JCM Wink compendium

Links

Streptomyces diastaticus Duché is a spore-forming, mesophilic prokaryote that builds an aerial mycelium.

spore-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 389280

ATCC 10975,ATCC 25455,IFO 3198,IFO 13043,DSM 40324,JCM 4136,CCRC 12105,IMET 40289,NRRL B-1344,NRRL B-1903,VKM Ac-713,JCM 4478,NBRC 13043,NBRC 3198,AS 4.1854,BCRC 12105,CGMCC 4.1885,CGMCC 4.2081,CGMCC 4.1854,AS 4.1885,CBS 422.34

@ref 20215

Last LPSN update 2025-12-16 09:50:33

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces diastaticus

Full scientific name Streptomyces diastaticus (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980)

Synonyms (6)

Streptomyces gougerotii
Streptomyces gougeroti
"Actinomyces gougeroti"
"Actinomyces diastaticus"
"Actinomyces rutgersensis"
Streptomyces rutgersensis

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99.5
125438		90.5

Colony morphology

@ref	Colony color	Incubation period	Medium used
19437	Yellowish-brown	10-14 days	ISP 2
19437	White	10-14 days	ISP 3
19437	Yellow	10-14 days	ISP 4
19437	Yellow-brown	10-14 days	ISP 5

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19437	Aerial Mycelium	Yellow/Reddish	ISP 2
19437	Aerial Mycelium	White	ISP 3
19437	Aerial Mycelium	White	ISP 4
19437	Aerial Mycelium		ISP 5

Multimedia

@ref:	9462
multimedia content:	DSM_40324.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40324.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9462	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
9462	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19437	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19437	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19437	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19437	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
9462	positive	growth	28	mesophilic
19437	positive	optimum	30	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.8

Spore formation

@ref	Spore description	Spore formation
19437	Formation of spore chains: rectiflixibilis, spore surface: smooth
125439
125438

Compound production

@ref	Compound
9462	gougerotin
20216	Gougerotin

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19437	NaCl	positive	maximum	5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
19437	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	-	from API 20E
19437	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	from API 20E
19437	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	from API 20E
19437	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	from API 20E
19437	29864 ChEBI	mannitol	+
19437	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	-	from API 20E
19437	16634 ChEBI	raffinose	-
19437	26546 ChEBI	rhamnose	-
19437	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	from API 20E
68368	16199 ChEBI	urea	+	from API 20E
19437	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68368	15688 ChEBI	acetoin	+	from API 20E
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19437	-	-	-	-	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9462	1	Risk group (German classification) According to TRBA 466
19437	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM1117014v1 assembly for Streptomyces gougerotii NBRC 13043	contig	GCA_011170145	53448.3	8112156458	53448	66.53
66792	ASM1464895v1 assembly for Streptomyces gougerotii JCM 4136	contig	GCA_014648955	53448.4	8109771594	53448	63.28

Genome browser: GCA_011170145

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	S.gougerotii (DSM 40324T) 16S rRNA gene	Z76687	1476	53448
20218	Streptomyces gougerotii gene for 16S ribosomal RNA, partial sequence, strain: JCM 4478	D44173	120	53448
20218	Streptomyces gougerotii gene for 16S rRNA, partial sequence, strain: NBRC 13043	AB249982	1452	53448
9462	Streptomyces gougerotii gene for 16S rRNA, partial sequence, strain: NBRC 3198	AB184742	1478	53448
124043	Streptomyces gougerotii strain JCM 4136 16S ribosomal RNA gene, partial sequence.	MT760485	1300	53448
124043	Streptomyces gougerotii strain NBRC 13043 16S ribosomal RNA gene, partial sequence.	MW452994	907	53448
124043	Streptomyces gougerotii strain NBRC 13043 16S ribosomal RNA gene, partial sequence.	KY087980	1442	53448

GC content

@ref	GC-content (mol%)	Method
67770	71	fluorimetric
67770	73.6	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Streptomyces gougerotii JCM 4136
NCBI: GCA_014648955

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	97.10	no
125439	motility	BacteriaNetⓘ	no	89.70	no
125439	gram_stain	BacteriaNetⓘ	positive	99.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces gougerotii strain JCM 4136
PATRIC: 53448.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.58	no
125438	anaerobic	anaerobicⓘ	no	97.39	no
125438	spore-forming	spore-formingⓘ	yes	94.33	yes
125438	aerobic	aerobicⓘ	yes	89.88	no
125438	thermophilic	thermophileⓘ	no	95.50	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Title	Authors	Journal	DOI	Year
Electron microscopy of Streptomyces spore morphology and its role in species differentiation.	TRESNER HD, DAVIES MC, BACKUS EJ.	J Bacteriol	10.1128/jb.81.1.70-80.1961	1961
Genomic insights into biosynthesis and adaptation in the bioactive marine bacterium Streptomyces albidoflavus VIP-1 from the Red Sea.	Sedeek AM, Elfeky H, Hanora AS, Solyman SM.	BMC Microbiol	10.1186/s12866-025-04109-x	2025
Resistance to and accumulation of heavy metals by actinobacteria isolated from abandoned mining areas.	El Baz S, Baz M, Barakate M, Barakate M, Hassani L, Hassani L, El Gharmali A, Imziln B.	ScientificWorldJournal	10.1155/2015/761834	2015
Reclassification of Streptomyces diastaticus subsp. ardesiacus, Streptomyces gougerotii and Streptomyces rutgersensis.	Komaki H, Tamura T.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004287	2020
Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger).	Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R, Zhang Y, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-013-0021-3	2013

References

#9462	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40324
#19437	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces diastaticus (DSM 40324, AS 4.1854, ATCC 10975)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_011170145

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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