Streptomyces rochei (DSM 40742, ATCC 15303, CBS 546.70)

Name: Streptomyces rochei (DSM 40742, ATCC 15303, CBS 546.70)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15219

Type strain

Strain Designation FH 1278

Culture col. no. DSM 40742 ATCC 15303 CBS 546.70 JCM 4962 FH 1278 6 more

NCBI tax ID(s) 68207

Special Collections DSMZ JCM Wink compendium

History DSMZ <- Hoechst AG, FH 1278 JCM <-- KCC S-0962 <-- DSM 40742 <-- Farbwerke Hoechst AG, FH 1278.

Links

Streptomyces rochei FH 1278 is a spore-forming bacterium that builds an aerial mycelium and produces antibiotic compounds.

antibiotic compound production spore-forming genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 389357

ATCC 15303,DSM 40742,JCM 4962,IFO 15413,NRRL B-12102,VKM Ac-844,CBS 546.70,CECT 3209,KCTC 9883,NBRC 15413,CGMCC 4.1966

@ref 20215

Last LPSN update 2026-05-29 08:28:29

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces rochei

Full scientific name Streptomyces rochei Berger et al. 1953 (Approved Lists 1980)

Synonyms (4)

Streptomyces enissocaesilis
Streptomyces plicatus
Streptomyces vinaceusdrappus
Streptomyces geysiriensis

BacDive ID	Other strains from **Streptomyces rochei** (10)	Type strain
15553	S. rochei X-15, DSM 40231, ATCC 10739, ATCC 19245, ... (type strain)
15554	S. rochei K-5, DSM 40913
15555	S. rochei 5E, ID 3818, DSM 41711
15556	S. rochei 17G, DSM 41722
15557	S. rochei 31A, DSM 41729
15558	S. rochei 33B, DSM 41732
15559	S. rochei 34B, ID 3846, DSM 41735
15560	S. rochei 35A, ID 3848, DSM 41737
15561	S. rochei JA 9767, JA 9769, DSM 46472, IMET 43107
159109	S. rochei DSM 40916

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125439		92.338
125439		98.944	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
18666	Green brown (8000)	10-14 days	ISP 2
18666	Beige (1001)	10-14 days	ISP 3
18666	Yellow	10-14 days	ISP 4
18666	Beige (1001)	10-14 days	ISP 5
18666	Yellow	10-14 days	ISP 6
18666	Brown	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
18666	Aerial Mycelium	Grey	ISP 2
18666	Aerial Mycelium	Grey	ISP 3
18666	Aerial Mycelium	White	ISP 4
18666	Aerial Mycelium	Beige	ISP 5
18666			ISP 6
18666	Aerial Mycelium	Grey	ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9761	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18666	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18666	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18666	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18666	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18666	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18666	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9761	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 20.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l NaCl 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
9761	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
9761	positive	growth	37
18666	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.645

Spore formation

@ref	Spore description	Confidence
18666	Formation of spore chains (spirales)(Sp), spore surface hairy
125439		90.689
125438		92.842

Compound production

9761

Compoundmoenomycin

Halophily

@ref	Salt	Growth	Tested relation	Concentration
18666	NaCl	positive	maximum	5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18666	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
18666	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
18666	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
18666	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
18666	29864 ChEBI	mannitol	+
18666	17268 ChEBI	myo-inositol	+/-
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
18666	16634 ChEBI	raffinose	-
18666	26546 ChEBI	rhamnose	+
18666	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
18666	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
67770	28908 ChEBI	bambermycin
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	-		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18666	+	-	-	-	-	-	+	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
9761	soil
67770	Soil	a geyser	Iceland	ISL	Europe

Taxonmaps

69479

Global distribution of 16S sequence AB184661 (>99% sequence identity) for Streptomyces from Microbeatlas

Aquatic Samples	0
Soil Samples	12
Animal Samples	16
Plant Samples	0
Total Samples	28

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9761	1	Risk group (German classification) According to TRBA 466
18666	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM1465109v1 assembly for Streptomyces geysiriensis JCM 4962	scaffold	GCA_014651095	68207.3	8109687602	68207	24.8

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces geysiriensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 4962	D44421	121	68207
20218	Streptomyces geysiriensis gene for 16S rRNA, partial sequence, strain: NBRC 15413	AB184661	1477	68207
20218	Streptomyces geysiriensis strain NRRL B-12102T 16S ribosomal RNA gene, partial sequence	DQ442501	1480	68207
124043	Streptomyces geysiriensis strain JCM 4962 16S ribosomal RNA gene, partial sequence.	MT760640	821	68207
124043	Streptomyces geysiriensis strain NBRC 15413(T) 16S ribosomal RNA gene, partial sequence.	MN686681	582	68207

GC content

@ref	GC-content (mol%)	Method
67770	72.6	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1465109v1 assembly for Streptomyces geysiriensis JCM 4962
NCBI: GCA_014651095

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	positive	98.94	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.65	no
125439	motility	BacteriaNetⓘ	no	92.34	no
125439	spore_formation	BacteriaNetⓘ	yes	90.69	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces geysiriensis strain JCM 4962
PATRIC: 68207.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.80	no
125438	anaerobic	anaerobicⓘ	no	97.81	no
125438	spore-forming	spore-formingⓘ	yes	92.84	no
125438	aerobic	aerobicⓘ	yes	86.56	no
125438	thermophilic	thermophileⓘ	no	95.00	no
125438	flagellated	motile2+ⓘ	no	87.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Halotolerant Marine Rhizosphere-Competent Actinobacteria Promote Salicornia bigelovii Growth and Seed Production Using Seawater Irrigation.	Mathew BT, Torky Y, Amin A, Mourad AI, Ayyash MM, El-Keblawy A, Hilal-Alnaqbi A, AbuQamar SF, El-Tarabily KA.	Front Microbiol	10.3389/fmicb.2020.00552	2020
Phylogeny	Reclassification of 15 Streptomyces species as synonyms of Streptomyces albogriseolus, Streptomyces althioticus, Streptomyces anthocyanicus, Streptomyces calvus, Streptomyces griseoincarnatus, Streptomyces mutabilis, Streptomyces pilosus or Streptomyces rochei.	Komaki H.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004718	2019
Pathogenicity	Screening of endophytic Streptomycetes isolated from Parthenium hysterophorus L. against nosocomial pathogens.	Tanvir R, Sajid I, Hasnain S.	Pak J Pharm Sci		2013
	Rootstock Priming with Shikimic Acid and Streptomyces griseus for Growth, Productivity, Physio-Biochemical, and Anatomical Characterisation of Tomato Grown under Cold Stress.	Sayed EG, Mahmoud AWM, Abdel-Wahab A, El-Bahbohy RM, Azoz SN.	Plants (Basel)	10.3390/plants11212822	2022
	Cliona varians-Derived Actinomycetes as Bioresources of Photoprotection-Related Bioactive End-Products.	Sanchez-Suarez J, Villamil L, Coy-Barrera E, Diaz L.	Mar Drugs	10.3390/md19120674	2021
Metabolism	A Bioactive Fraction from Streptomyces sp. Enhances Maize Tolerance against Drought Stress.	Warrad M, Hassan YM, Mohamed MSM, Hagagy N, Al-Maghrabi OA, Selim S, Saleh AM, AbdElgawad H.	J Microbiol Biotechnol	10.4014/jmb.2003.03034	2020

References

#9761	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40742
#18666	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Streptomyces rochei (DSM 40742, ATCC 15303, CBS 546.70)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014651095

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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