Burkholderia ambifaria (CCUG 45121, CEP 0996, LMG 19467)

Name: Burkholderia ambifaria (CCUG 45121, CEP 0996, LMG 19467)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 151891

Type strain

Culture col. no. CCUG 45121 CEP 0996 LMG 19467

NCBI tax ID(s) 152480

Special Collections CCUG

Links

Burkholderia ambifaria CCUG 45121 is an aerobe, mesophilic prokaryote that was isolated from Human sputum ?,cystic fibrosis.

aerobe mesophilic genome sequence

Name and taxonomic classification

SI-ID 13236

LMG 19467,CCUG 45121

@ref 20215

Last LPSN update 2025-12-16 10:06:27

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Burkholderia

Species Burkholderia ambifaria

Full scientific name Burkholderia ambifaria Coenye et al. 2001

BacDive ID	Other strains from **Burkholderia ambifaria** (2)	Type strain
1946	B. ambifaria DSM 16087, CCUG 44356, CIP 107266, LMG 19182 (type strain)
176833	B. ambifaria CT11-114, DSM 115947

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	97.5

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
56655	positive	growth	30-37	mesophilic

Physiology and metabolism

Oxygen tolerance

56655

Oxygen toleranceaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	+	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68369	gelatinase	+		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

56655

Fatty acid	Percentage	ECL
C16:0	22.7	16
C17:0 CYCLO	18.9	16.888
C16:1 ω7c	15.3	15.819
C18:1 ω7c /12t/9t	13.9	17.824
C14:0 3OH/C16:1 ISO I	6.5	15.485
C16:0 3OH	5.1	17.52
C14:0	4.7	14
C18:1 2OH	3.6	19.088
C19:0 CYCLO ω8c	2.5	18.9
C16:1 2OH	2	17.047
C16:0 2OH	1.3	17.233
C12:0	1.1	12
C16:1 ω5c	1	15.908
C15:0	1	15
C15:1 ω6c	0.5	14.856

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
56655	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	+	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
56655	+	-	+	-	-	-	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum
#Infection	#Disease	#Cystic fibrosis
#Infection	#Patient	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
56655	Human sputum ?,cystic fibrosis	Australia	AUS	Australia and Oceania

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM2668383v2 assembly for Burkholderia ambifaria CEP0996	complete	GCA_026683835	152480.137	152480	99.3
66792	ASM2668379v2 assembly for Burkholderia ambifaria CEP0996 pc3-null	chromosome	GCA_026683795	152480.139	152480	87.7

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Burkholderia ambifaria CEP0996 pc3-null
NCBI: GCA_026683795.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.50	no
125438	anaerobic	anaerobicⓘ	no	96.92	yes
125438	aerobic	aerobicⓘ	yes	88.38	yes
125438	spore-forming	spore-formingⓘ	no	87.65	no
125438	thermophilic	thermophileⓘ	no	98.00	no
125438	flagellated	motile2+ⓘ	yes	82.90	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Cultivation	Evaluation of Three Culture Media for Isolation of Burkholderia cepacia Complex from Respiratory Samples of Patients with Cystic Fibrosis.	Marrs ECL, Perry A, Perry JD.	Microorganisms	10.3390/microorganisms9122604	2021
Pathogenicity	Advances in Phage Therapy: Targeting the Burkholderia cepacia Complex.	Lauman P, Dennis JJ.	Viruses	10.3390/v13071331	2021
Pathogenicity	Antibacterial activity of a lectin-like Burkholderia cenocepacia protein.	Ghequire MG, De Canck E, Wattiau P, Van Winge I, Loris R, Coenye T, De Mot R.	Microbiologyopen	10.1002/mbo3.95	2013
	Effect of beta-Lactamase inhibitors on in vitro activity of beta-Lactam antibiotics against Burkholderia cepacia complex species.	Everaert A, Coenye T.	Antimicrob Resist Infect Control	10.1186/s13756-016-0142-3	2016
Phylogeny	Development of a species-specific fur gene-based method for identification of the Burkholderia cepacia complex.	Lynch KH, Dennis JJ, Dennis JJ.	J Clin Microbiol	10.1128/jcm.01460-07	2008
Enzymology	Low rates of Pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients.	Kidd TJ, Ramsay KA, Hu H, Bye PT, Elkins MR, Grimwood K, Harbour C, Marks GB, Nissen MD, Robinson PJ, Rose BR, Sloots TP, Wainwright CE, Bell SC, ACPinCF Investigators.	J Clin Microbiol	10.1128/jcm.00014-09	2009
Phylogeny	Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex.	Baldwin A, Mahenthiralingam E, Thickett KM, Honeybourne D, Maiden MC, Govan JR, Speert DP, Lipuma JJ, Vandamme P, Dowson CG.	J Clin Microbiol	10.1128/jcm.43.9.4665-4673.2005	2005
Pathogenicity	Identification of a novel virulence factor in Burkholderia cenocepacia H111 required for efficient slow killing of Caenorhabditis elegans.	Huber B, Feldmann F, Kothe M, Vandamme P, Wopperer J, Riedel K, Eberl L.	Infect Immun	10.1128/iai.72.12.7220-7230.2004	2004
Pathogenicity	Biocide susceptibility of the Burkholderia cepacia complex.	Rose H, Baldwin A, Dowson CG, Mahenthiralingam E.	J Antimicrob Chemother	10.1093/jac/dkn540	2009

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#56655	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45121
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Burkholderia ambifaria (CCUG 45121, CEP 0996, LMG 19467)

Name and taxonomic classification