Streptomyces flavofungini (DSM 40366, ATCC 27430, CBS 411.59)

Name: Streptomyces flavofungini (DSM 40366, ATCC 27430, CBS 411.59)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15161

Type strain

Strain Designation Medipex SA-IX-3

Culture col. no. DSM 40366 ATCC 27430 CBS 411.59 CBS 672.72 IFO 13371 9 more

NCBI tax ID(s) 68200

Special Collections DSMZ JCM Wink compendium

History <- E.B. Shirling, ISP <- CBS <- Uri, Medipex SA-IX-3 KCC S-0753 <-- IFO 13371 <-- SAJ <-- ISP 5366 <-- CBS 411.59 <-- J. Uri and I. Békési SA-IX-3.

Links

Streptomyces flavofungini Medipex SA-IX-3 is a spore-forming bacterium that builds an aerial mycelium and was isolated from desert sand.

spore-forming genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 45803

ATCC 27430,IFO 13371,DSM 40366,JCM 4753,NRRL B-12307,VKM Ac-1179,CBS 411.59,LMG 8598,NBRC 13371,UAMH 6125,BCRC 15154,CCRC 15154

@ref 20215

Last LPSN update 2026-02-09 11:21:37

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces flavofungini

Full scientific name Streptomyces flavofungini (ex Úri and Békési 1958) Szabó and Preobrazhenskaya 1986

BacDive ID	Other strains from **Streptomyces flavofungini** (1)	Type strain
127766	S. flavofungini ST004997(HKI), 226, JA10075, IMET 40224

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	100
125439		94.7
125438	positive	91.97

Colony morphology

@ref	Colony color	Incubation period	Medium used
19446	Yellow	10-14 days	ISP 2
19446		10-14 days	ISP 3
19446	Yellow	10-14 days	ISP 4
19446	Yellow	10-14 days	ISP 5
19446	Yellow	10-14 days	ISP 6
19446	Yellow	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19446			ISP 2
19446	Aerial Mycelium	White	ISP 3
19446	Aerial Mycelium	White	ISP 4
19446			ISP 5
19446			ISP 6
19446	Aerial Mycelium	White	ISP 7

Multimedia

@ref:	9286
multimedia content:	DSM_40366.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40366.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9286	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19446	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19446	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19446	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19446	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19446	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19446	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9286	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 20.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l NaCl 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
9286	positive	growth	28
19446	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.4

Spore formation

@ref	Spore description	Confidence
19446	spore chains: very short, spore surface: smooth
125439		96.5
125438		93.742

Compound production

@ref	Compound
9286	flavofungin
20216	Flavofungin

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19446	NaCl	positive	maximum	5 %

Observation

67770

Observationquinones: MK-9(H₈), MK-9(H₆)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19446	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19446	62968 ChEBI	cellulose	+
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19446	28757 ChEBI	fructose	+/-
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19446	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19446	29864 ChEBI	mannitol	+
19446	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19446	16634 ChEBI	raffinose	-
19446	26546 ChEBI	rhamnose	-
19446	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19446	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
67770		flavofungin
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19446	+	+	+	+	-	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Desert
#Environmental	#Terrestrial	#Sandy
#Condition	#Xerophilic	-

Isolation

@ref	Sample type
9286	desert sand
67770	Desert sand

Taxonmaps

69479

Global distribution of 16S sequence AB184359 (>99% sequence identity) for Streptomyces from Microbeatlas

Aquatic Samples	4
Soil Samples	70
Animal Samples	16
Plant Samples	1
Total Samples	91

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9286	1	Risk group (German classification) According to TRBA 466
19446	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1465081v1 assembly for Streptomyces flavofungini JCM 4753	scaffold	GCA_014650815	68200.3	2990005190	68200	63.32
66792	ASM1641176v1 assembly for Streptomyces flavofungini JCM 4753	scaffold	GCA_016411765	68200.4	8110015461	68200	53.35

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces flavofungini gene for 16S ribosomal RNA, partial sequence, strain: JCM 4753	D44335	120	68200
20218	Streptomyces flavofungini gene for 16S rRNA, partial sequence, strain: NBRC 13371	AB184359	1475	68200
20218	Streptomyces flavofungini strain NRRL B-12307 16S ribosomal RNA gene, partial sequence	AY999792	1078	68200
124043	Streptomyces flavofungini strain JCM 4753 16S ribosomal RNA gene, partial sequence.	MT760606	1355	68200

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Streptomyces flavofungini JCM 4753
NCBI: GCA_014650815

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	96.50	no
125439	motility	BacteriaNetⓘ	no	94.70	no
125439	gram_stain	BacteriaNetⓘ	positive	100.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces flavofungini JCM 4753
NCBI: GCA_014650815.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.97	no
125438	anaerobic	anaerobicⓘ	no	96.36	no
125438	aerobic	aerobicⓘ	yes	88.63	no
125438	spore-forming	spore-formingⓘ	yes	93.74	yes
125438	thermophilic	thermophileⓘ	no	97.50	yes
125438	flagellated	motile2+ⓘ	no	87.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Discovery of anti-Mycobacterium tuberculosis desertomycins from Streptomyces flavofungini TRM90047 based on genome mining and HSQC-TOCSY.	Wang L, Reheman A, Wan C.	Sci Rep	10.1038/s41598-024-65702-0	2024
	Screening of Microbial Fermentation Products for Anti-M. tuberculosis Activity.	Reheman A, Lu D, Wang Y, Chen X, Cao G, Wan C.	Animals (Basel)	10.3390/ani12151947	2022
	Isolation and characterisation of rhizosphere bacteria active against Meloidogyne incognita, Phytophthora nicotianae and the root knot-black shank complex in tobacco.	Huang Y, Ma L, Fang DH, Xi JQ, Zhu ML, Mo MH, Zhang KQ, Ji YP.	Pest Manag Sci	10.1002/ps.3820	2015
Phylogeny	Streptomyces griseus 45H, a producer of the extracellular autoregulator protein factor C, is a member of the species Streptomyces albidoflavus.	Kiss Z, Ward AC, Birko Z, Chater KF, Biro S.	Int J Syst Evol Microbiol	10.1099/ijs.0.65311-0	2008
Pathogenicity	Desertomycin G, a New Antibiotic with Activity against Mycobacterium tuberculosis and Human Breast Tumor Cell Lines Produced by Streptomyces althioticus MSM3, Isolated from the Cantabrian Sea Intertidal Macroalgae Ulva sp.	Brana AF, Sarmiento-Vizcaino A, Perez-Victoria I, Martin J, Otero L, Palacios-Gutierrez JJ, Fernandez J, Mohamedi Y, Fontanil T, Salmon M, Cal S, Reyes F, Garcia LA, Blanco G.	Mar Drugs	10.3390/md17020114	2019
	Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique.	Mohamed OG, Dorandish S, Lindow R, Steltz M, Shoukat I, Shoukat M, Chehade H, Baghdadi S, McAlister-Raeburn M, Kamal A, Abebe D, Ali K, Ivy C, Antonova M, Schultz P, Angell M, Clemans D, Friebe T, Sherman D, Casper AM, Price PA, Tripathi A.	Mar Drugs	10.3390/md19080424	2021
Metabolism	Novel fermentation procedures for the production and the isolation of antibiotics.	Uri JV.	Acta Microbiol Hung		1986
Phylogeny	Streptomyces typhae sp. nov., a novel endophytic actinomycete with antifungal activity isolated the root of cattail (Typha angustifolia L.).	Peng C, Zhuang X, Gao C, Wang Z, Zhao J, Huang SX, Liu C, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-021-01561-3	2021
Phylogeny	Streptomyces xylanilyticus sp. nov., isolated from soil.	Moonmangmee D, Kanchanasin P, Phongsopitanun W, Tanasupawat S, Moonmangmee S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002282	2017
Phylogeny	Streptomyces lasiicapitis sp. nov., an actinomycete that produces kanchanamycin, isolated from the head of an ant (Lasius fuliginosus L.).	Ye L, Zhao S, Li Y, Jiang S, Zhao Y, Li J, Yan K, Wang X, Xiang W, Liu C	Int J Syst Evol Microbiol	10.1099/ijsem.0.001756	2017
Phylogeny	Streptomyces alfalfae sp. nov. and comparisons with its closest taxa Streptomyces silaceus, Streptomyces flavofungini and Streptomyces intermedius.	She W, Sun Z, Yi L, Zhao S, Liang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.000671	2015
Phylogeny	Streptomyces tacrolimicus sp. nov., a low producer of the immunosuppressant tacrolimus (FK506).	Martinez-Castro M, Barreiro C, Romero F, Fernandez-Chimeno RI, Martin JF	Int J Syst Evol Microbiol	10.1099/ijs.0.024273-0	2010

References

#9286	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40366
#19446	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces flavofungini (DSM 40366, ATCC 27430, CBS 411.59)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014650815

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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