Streptomyces flaveolus (DSM 40061, ATCC 19754, ATCC 25460)

Name: Streptomyces flaveolus (DSM 40061, ATCC 19754, ATCC 25460)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15154

Status isotype of 15155

Type strain

Strain Designation Actinomyces 168

Culture col. no. DSM 40061 ATCC 19754 ATCC 25460 ATCC 3319 CBS 493.68 36 more

NCBI tax ID(s) 67297

Special Collections DSMZ JCM KCTC Wink compendium JMRC

History DSMZ <- E.B. Shirling, ISP <- S.A. Waksman, IMRU JCM <-- KCC S-0032 <-- T. Yamaguchi (IAM 0005) <-- MTHU 65 <-- Hyden Chem. Corp. <-- ATCC 3319 <-- IMRU 3319.

Links

Streptomyces flaveolus Actinomyces 168 is a spore-forming bacterium that builds an aerial mycelium and produces amino acids.

amino acid production spore-forming genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 45808

LMG 19328,ATCC 19754,ATCC 3319,CCM 3171,CCRC 12489,IFO 12768,IFO 3408,IFO 3715,IMET 40233,JCM 4032,JCM 4577,NRRL B-1334,NRRL B-2688,VKM Ac-965,CBS 493.68,CBS 128.20,AS 4.1432,CECT 3181,KCTC 9022,IAM 0005,NBRC 12768,NBRC 3408,NBRC 3715,ICMP 392,BCRC 12489,IAM 0117,HAMBI 893,ICMP 741,CGMCC 4.1432,DSM 40061

@ref 20215

Last LPSN update 2026-05-29 08:28:28

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces flaveolus

Full scientific name Streptomyces flaveolus (Waksman 1923) Waksman and Henrici 1948 (Approved Lists 1980)

Synonyms (1)

"Actinomyces flaveolus"

BacDive ID	Other strains from **Streptomyces flaveolus** (5)	Type strain
15155	S. flaveolus Actinomyces 168, DSM 40328, ATCC 25460, ATCC ... (type strain)
15156	S. flaveolus Tonolo S-5018, Tü 55, DSM 40719, JCM 4983
15157	S. flaveolus 6585, DSM 41125, IMRU 3678
15158	S. flaveolus JA 4445, DSM 46369, IMET 40234, BUCSAV 6,7
127182	S. flaveolus ST003929(HKI), BI 06882,

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125439	positive	98.166
125439		92.659

Colony morphology

@ref	Colony color	Incubation period	Medium used
19396	Yellow	10-14 days	ISP 2
19396	Yellow	10-14 days	ISP 3
19396	Yellow	10-14 days	ISP 4
19396	Yellow	10-14 days	ISP 5
19396	Yellow	10-14 days	ISP 6
19396	Yellow	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19396	Aerial Mycelium	Grey	ISP 2
19396	Aerial Mycelium	Grey	ISP 3
19396	Aerial Mycelium	Grey	ISP 4
19396	Aerial Mycelium	Grey	ISP 5
19396			ISP 6
19396	Aerial Mycelium	White	ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9243	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19396	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19396	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19396	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19396	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19396	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19396	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9243	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 20.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l NaCl 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
9243	positive	growth	28
19396	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.333

Spore formation

@ref	Spore description	Spore formation	Confidence
19396	Formation of spore chains: rectiflixibilis, spore surface: hairy
125438			93.194

Compound production

9243

CompoundL isoleucine

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19396	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
19396	62968 ChEBI	cellulose	+
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19396	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19396	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19396	29864 ChEBI	mannitol	+
19396	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19396	16634 ChEBI	raffinose	+
19396	26546 ChEBI	rhamnose	+
19396	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19396	18222 ChEBI	xylose	+

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	+	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19396	+	-	-	-	-	-	+	+	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

9243

Sample typesoil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9243	1	Risk group (German classification) According to TRBA 466
19396	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1464881v1 assembly for Streptomyces flaveolus JCM 4032	contig	GCA_014648815	67297.6	8112957189	67297	69.65

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces flaveolus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4577	D44245	121	67297
20218	Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 12768	AB184131	1463	67297
20218	Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 3408	AB184764	1477	67297
20218	Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 3715	AB184786	1477	67297
20218	Streptomyces flaveolus strain NRRL B-1334 16S ribosomal RNA gene, partial sequence	EF654098	1481	67297
20218	Streptomyces flaveolus strain NRRL B-2688 16S ribosomal RNA gene, partial sequence	AY999799	1192	67297
124043	Streptomyces flaveolus strain JCM 4032 16S ribosomal RNA gene, partial sequence.	MT760473	1371	67297
124043	Streptomyces flaveolus strain CCM 3171 16S ribosomal RNA gene, partial sequence.	MT760095	1348	67297

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1464881v1 assembly for Streptomyces flaveolus JCM 4032
BV-BRC: 67297.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.33	no
125439	gram_stain	BacteriaNetⓘ	positive	98.17	no
125439	spore_formation	BacteriaNetⓘ	yes	89.15	no
125439	motility	BacteriaNetⓘ	no	92.66	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces flaveolus strain JCM 4032
PATRIC: 67297.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.42	no
125438	anaerobic	anaerobicⓘ	no	97.89	no
125438	spore-forming	spore-formingⓘ	yes	93.19	yes
125438	aerobic	aerobicⓘ	yes	89.70	no
125438	thermophilic	thermophileⓘ	no	96.50	no
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Mutation in Streptomyces flaveolus Induced by X-rays and Ultraviolet Light.	Kelner A.	J Bacteriol	10.1128/jb.56.4.457-465.1948	1948
	Ultrastructural studies of sporulation in Streptomyces.	Hardisson C, Manzanal MB.	J Bacteriol	10.1128/jb.127.3.1443-1454.1976	1976
	Nucleotide sequence of afsB, a pleiotropic gene involved in secondary metabolism in Streptomyces coelicolor A3(2) and "Streptomyces lividans".	Horinouchi S, Suzuki H, Beppu T.	J Bacteriol	10.1128/jb.168.1.257-269.1986	1986
Phylogeny	Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method.	Zheng BX, Ibrahim M, Zhang DP, Bi QF, Li HZ, Zhou GW, Ding K, Penuelas J, Zhu YG, Yang XR.	AMB Express	10.1186/s13568-018-0575-6	2018
	Undescribed ansatrienin analogs from Streptomyces flaveolus including two N-acetylcysteine conjugates	Zhu X, Wu X, Li H, Zhang M, Yu D, Wang Z, Huang Q, Chu Z, Sun P.	Phytochemistry.		2025
	Undescribed ansatrienin analogs from Streptomyces flaveolus including two N-acetylcysteine conjugates.	Zhu X, Wu X, Li H, Zhang M, Yu D, Wang Z, Huang Q, Chu Z, Sun P.	Phytochemistry	10.1016/j.phytochem.2025.114598	2025
	Ansafurantrienins, Unprecedented Ansatrienin Derivatives Formed via Photocatalytic Intramolecular [3 + 2] Oxidative Cycloaddition.	Li H, Chen S, Wang J, Zhang M, Wu W, Liu W, Sun P.	Org Lett	10.1021/acs.orglett.1c04032	2022
	Bioinformatic and Functional Evaluation of Actinobacterial Piperazate Metabolism.	Hu Y, Qi Y, Stumpf SD, D'Alessandro JM, Blodgett JAV.	ACS Chem Biol	10.1021/acschembio.8b01086	2019
	Isolation of antimicrobial producing Actinobacteria from soil samples.	Elbendary AA, Hessain AM, El-Hariri MD, Seida AA, Moussa IM, Mubarak AS, Kabli SA, Hemeg HA, El Jakee JK.	Saudi J Biol Sci	10.1016/j.sjbs.2017.05.003	2018
	Deciphering core microbiota in rhizosphere soil and roots of healthy and Rhizoctonia solani-infected potato plants from various locations.	Yang Y, Hu J, Wei X, Huang K, Li C, Yang G.	Front Microbiol	10.3389/fmicb.2024.1386417	2024
Genetics	Exploration of genomic and functional features of chitinolytic bacterium Streptomyces chilikensis RC1830, isolated from Chilika Lake, India.	Tanaya Behera H, Mojumdar A, Kumari K, Kumar Gouda S, Das S, Ray L.	3 Biotech	10.1007/s13205-022-03184-5	2022
	Generation of a high quality library of bioactive filamentous actinomycetes from extreme biomes using a culture-based bioprospecting strategy.	Swiecimska M, Golinska P, Goodfellow M.	Front Microbiol	10.3389/fmicb.2022.1054384	2022
	Proteomining-Based Elucidation of Natural Product Biosynthetic Pathways in Streptomyces.	Linardi D, She W, Zhang Q, Yu Y, Qian PY, Lam H.	Front Microbiol	10.3389/fmicb.2022.913756	2022
	Discovery of unusual dimeric piperazyl cyclopeptides encoded by a Lentzea flaviverrucosa DSM 44664 biosynthetic supercluster.	Li C, Hu Y, Wu X, Stumpf SD, Qi Y, D'Alessandro JM, Nepal KK, Sarotti AM, Cao S, Blodgett JAV.	Proc Natl Acad Sci U S A	10.1073/pnas.2117941119	2022
Phylogeny	Streptomyces chilikensis sp. nov., a halophilic streptomycete isolated from brackish water sediment.	Ray L, Suar M, Pattnaik AK, Raina V	Int J Syst Evol Microbiol	10.1099/ijs.0.046284-0	2013

References

#9243	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40061
#19396	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces flaveolus (DSM 40061, ATCC 19754, ATCC 25460)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014648815

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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