Streptomyces phaeochromogenes (DSM 40741, ATCC 15304, JCM 4958)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	9760
  multimedia content:	DSM_40741.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40741.jpg
  caption:	Medium 65 28°C
  intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
9760	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18665	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18665	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18665	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18665	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18665	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18665	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18665	22599 ChEBI	arabinose	+/-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
18665	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
18665	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
18665	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
18665	29864 ChEBI	mannitol	+
18665	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
18665	16634 ChEBI	raffinose	+
18665	26546 ChEBI	rhamnose	+
18665	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
18665	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
67770	28908 ChEBI	bambermycin
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	+	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2464890v1 assembly for Streptomyces umbrinus CECT 3212	scaffold	GCA_024648905	67370.5	2863240559	67370	70.6
67770	ASM1465627v1 assembly for Streptomyces umbrinus JCM 4958	scaffold	GCA_014656275	83383.3	8113252029	67370	62.14

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Streptomyces umbrinus gene for 16S rRNA, partial sequence	AB018209	120		83383
20218	Streptomyces ederensis strain KCTC 9726 16S ribosomal RNA gene, partial sequence	AY999824	1420		83383
20218	Streptomyces umbrinus gene for 16S rRNA, partial sequence, strain: NBRC 15410	AB184658	1483		83383
20218	Streptomyces ederensis strain NRRL B-8146 16S ribosomal RNA gene, partial sequence	EU594481	1490		83383
124043	Streptomyces ederensis strain JCM 4958 16S ribosomal RNA gene, partial sequence.	MT760639	1406		67370

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics.	Charousova I, Medo J, Hleba L, Javorekova S.	Braz J Microbiol	10.1016/j.bjm.2017.12.007	2018
	Streptomyces phaeochromogenes BV-204, K-1115A Anthraquinone-Producing Strain: A New Protein Biosynthesis Inhibitor.	Belik AR, Zakalyukina YV, Alferova VA, Buyuklyan YA, Osterman IA, Biryukov MV.	Acta Naturae	10.32607/actanaturae.27315	2024
Phylogeny	Genomic insight into the Streptomyces aurantiacus clade: reclassification of Streptomyces ederensis as a later heterotypic synonym of Streptomyces umbrinus and Streptomyces glomeroaurantiacus as a later heterotypic synonym of Streptomyces aurantiacus.	Saygin H.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004797	2021
Phylogeny	Molecular and phenotypic comparison of phaeochromycin-producing strains of Streptomyces phaeochromogenes and Streptomyces ederensis.	Ritacco FV, Eveleigh DE.	J Ind Microbiol Biotechnol	10.1007/s10295-008-0367-0	2008
	Use of Endophytic and Rhizosphere Actinobacteria from Grapevine Plants To Reduce Nursery Fungal Graft Infections That Lead to Young Grapevine Decline.	Alvarez-Perez JM, Gonzalez-Garcia S, Cobos R, Olego MA, Ibanez A, Diez-Galan A, Garzon-Jimeno E, Coque JJR.	Appl Environ Microbiol	10.1128/aem.01564-17	2017
Phylogeny	Streptomyces liliifuscus sp. nov and an anti-ginger plague agent Streptomyces liliiviolaceus sp. nov, two novel species isolated from soil of Lilium lancifolium.	Li K, Man Y, Liu J, Liu Z, Ma H, Zhu H, Zhou Y, Zhang C, Zhou X	Int J Syst Evol Microbiol	10.1099/ijsem.0.005340	2022
Phylogeny	Two new species of the genus Streptomyces: Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. isolated from the cuticle of Camponotus japonicus Mayr.	Piao C, Zheng W, Li Y, Liu C, Jin L, Song W, Yan K, Wang X, Xiang W	Arch Microbiol	10.1007/s00203-017-1353-6	2017