Name: Actinomyces israelii DSM 43320
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 151

Type strain:

Species: Actinomyces israelii

Culture col. no.: DSM 43320, ATCC 12102, JCM 12964, CCUG 18307, IMET 10792, CCUG 2271, NIH 277, WVU 46, CIP 103259, KCTC 15377, NCTC 12972

Strain history: DSM 43320 <-- M. A. Gerencser WVU 46 <-- ATCC 12102 <-- A. Howell NIH 277.

NCBI tax ID(s): 1120944 (strain), 1659 (species)

Other strains from Actinomyces israelii

A. israelii CCUG 34702, DSM 43011, ATCC 10048, ...
A. israelii DSM 43323, WVU 339
A. israelii STI09064(IMET), 1110-009,
A. israelii STI09079(IMET), 1141-014,
A. israelii CIP 53.109
A. israelii CIP 104730, CCUG 4867, NCTC 4860
A. israelii CIP 60.40
A. israelii CIP 104876, NCTC 10236, CCUG 34502
A. israelii CIP 104874, NCTC 6826, CCUG 34495
A. israelii CCUG 34501, CIP 104875, NCTC 10215
A. israelii CCUG 8060
A. israelii CCUG 30217
A. israelii CCUG 33763
A. israelii CCUG 34284, ATCC 12597
A. israelii CCUG 34496, NCTC 6827
A. israelii CCUG 34497, NCTC 6830
A. israelii CCUG 34498, NCTC 6831
A. israelii CCUG 34499, NCTC 8047
A. israelii CCUG 34500, NCTC 8439
A. israelii CCUG 34556
A. israelii CCUG 34557
A. israelii CCUG 35455
A. israelii CCUG 35624
A. israelii CCUG 35922
A. israelii CCUG 35923
A. israelii CCUG 35924
A. israelii CCUG 35925
A. israelii CCUG 35926
A. israelii CCUG 36904
A. israelii CCUG 36905
A. israelii CCUG 37486 C
A. israelii CCUG 37496
A. israelii CCUG 47135
A. israelii CCUG 53438
A. israelii CCUG 54890
A. israelii CCUG 64147

Section

Actinomyces israelii DSM 43320 is an anaerobe, mesophilic bacterium that was isolated from human brain abscess.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Actinomycetales*
	Family	*Actinomycetaceae*
	Genus	*Actinomyces*
	Species	**Actinomyces israelii**
	Full Scientific Name (PNU)	Actinomyces israelii (Kruse 1896) Lachner-Sandoval 1898 (Approved Lists 1980)

	Synonym	"Actinobacterium* israeli"*
	Synonym	"Streptothrix* israeli"*

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %93.876

[Ref.: #46802]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #40615]

Culture medium

MEDIUM 39 - for Actinomyces

[Ref.: #40615]

Culture medium growth

[Ref.: #40615]

Culture medium composition

expand / minimize

[Ref.: #10954]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #10954]

Culture medium growth

[Ref.: #10954]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #10954]

Culture medium composition

expand / minimize

[Ref.: #10954]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #10954]

Culture medium growth

[Ref.: #10954]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #10954]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #10954]	growth	37 °C
[Ref.: #40615]	growth	37 °C
[Ref.: #46802]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #10954]	Temperature range	mesophilic
[Ref.: #40615]	Temperature range	mesophilic
[Ref.: #46802]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #10954]	Oxygen tolerance	anaerobe
[Ref.: #46802]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %94.264

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.989

[Ref.: #10954]	Murein short key	A11.52
[Ref.: #10954]	Murein types	A5alpha L-Lys-L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68377]	D-fructose ChEBI	+	builds acid from	* from API NH
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68377]	maltose ChEBI	+	builds acid from	* from API NH
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68377]	sucrose ChEBI	-	builds acid from	* from API NH
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A, API NH
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A, API NH, API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68380]		indole ChEBI	* from API rID32A, API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A, API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A, API NH
[Ref.: #68380]	alpha-arabinosidase	+	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A, API Coryne
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68377]	beta-galactosidase	-	3.2.1.23	* from API NH, API Coryne
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A, API Coryne
[Ref.: #68377]	beta-lactamase	-	3.5.2.6	* from API NH
[Ref.: #68379]	catalase	-	1.11.1.6	* from API Coryne
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A, API Coryne
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #68380]	phenylalanine arylamidase	+		* from API rID32A
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A, API NH
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A, API Coryne
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A, API NH
[Ref.: #68380]	tyrosine arylamidase	+		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API NH, API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #46802]	802	-	+	-	+	-	-	+	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	+	-	-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #46802]	1280	-	+	+	+	-	-	-	-	-	-	+	-	-

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #10954]	11578	-	-	-	+	-	+	+	+	-	-	+/-	+	-	-	+	-	-	-	+	-	+	+	-	+	-	-	-	-	-
[Ref.: #10954]	11577	-	-	+/-	+	-	+	+	+	-	-	+	+	-	-	-	-	-	-	+	-	+	+	-	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #10954]	Sample type/isolated from	human brain abscess

[Ref.: #46802]	Sample type/isolated from	Human brain abscess
[Ref.: #46802]	Geographic location (country and/or sea, region)	MN,Rochester
[Ref.: #46802]	Country	USA
[Ref.: #46802]	Country ISO 3 Code	USA
[Ref.: #46802]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Human brain abscess
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Brain
#Host Body-Site	#Other	#Abscess
#Infection	#Patient	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X82450 (>99% sequence identity) for Actinomyces israelii subclade from Microbeatlas

Aquatic Samples	282
Soil Samples	91
Animal Samples	11935
Plant Samples	50
Total Samples	12358

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #10954]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinomyces israelii strain ATCC 12102 16S ribosomal RNA gene, partial sequence	AF058042	280	ENA	1659 tax ID	*
[Ref.: #20218]	A.israelii 16S rRNA gene	X82450	1441	ENA	1659 tax ID	*
[Ref.: #20218]	Actinomyces israelii gene for 16S ribosomal RNA, partial sequence, strain: JCM 12964	AB849123	1512	ENA	1659 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinomyces israelii DSM 43320 DSM 43320	GCA_000711965	scaffold	GenBank	1120944 tax ID
[Ref.: #67770]	Actinomyces israelii strain NCTC12972 genome assembly, chromosome: 1	LR134357		ENA	1659 tax ID
[Ref.: #66792]	Actinomyces israelii DSM 43320	1120944.3	wgs	PATRIC	1120944 tax ID	*
[Ref.: #66792]	Actinomyces israelii strain NCTC12972	1659.4	complete	PATRIC	1659 tax ID	*
[Ref.: #66792]	Actinomyces israelii DSM 43320	2565956542	draft	JGI IMG/M	1120944 tax ID	*
[Ref.: #66792]	Actinomyces israelii NCTC12972	2843235134	complete	JGI IMG/M	1659 tax ID	*

[Ref.: #67770]

GC-content

70 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomyces israelii strain NCTC12972 PATRIC: 1659.4
[Ref.: #69480]	thermophile	no	99.957	yes
[Ref.: #69480]	gram-positive	yes	100	yes
[Ref.: #69480]	anaerobic	yes	94.264	no
[Ref.: #69480]	spore-forming	no	99.989	no
[Ref.: #69480]	motile	no	93.876	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinomyces israelii strain NCTC12972 PATRIC: 1659.4
[Ref.: #69481]	spore-forming	no	64	no

Availability in culture collections External links

[Ref.: #10954]

Culture collection no.

DSM 43320, ATCC 12102, JCM 12964, CCUG 18307, IMET 10792, CCUG 2271, NIH 277, WVU 46, CIP 103259, KCTC 15377, NCTC 12972

[Ref.: #20218]

Associated Passport(s) in StrainInfo

126953, 96054, 534331

Literature:

	Title	Authors	Journal	DOI	Year
Cultivation	Production of antagonistic substance by Eikenella corrodens isolated from the oral cavity of human beings with and without periodontal disease.	Apolonio AC, Carvalho MA, Ribas RN, Sousa-Gaia LG, Santos KV, Lana MA, Nicoli JR, Farias LM	J Appl Microbiol	10.1111/j.1365-2672.2006.03211.x	2007	*
	[Initial study on the discrimination of oral common Actinomycetes with metabonomics method].	Li MY, Guo Q, Zhou XD, Xiong P, Jia XM, Xiao XR, Li W, Xiao LY	Hua Xi Kou Qiang Yi Xue Za Zhi		2009	*
	[Transmission electron microscopic studies of fine structure by fast freeze method on Actinomyces israelii ATCC 12102].	Watanabe T, Matui T, Suzuki M	Shigaku		1989	*

References

#10954

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43320

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40615 ; Curators of the CIP;
#46802 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 18307

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68377

Automatically annotated from API NH .

#68379

Automatically annotated from API Coryne .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Actinomyces israelii strain NCTC12972

Actinomyces israelii strain NCTC12972

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