Name: Virgibacillus salarius SA-Vb 1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1486

Type strain:

Species: Virgibacillus salarius

Strain Designation: SA-Vb 1

Culture col. no.: DSM 18441, JCM 12946

Strain history: T. Naganuma SA-Vb1.

NCBI tax ID(s): 447199 (species)

SI-ID 404521

DSM 18441

Special Collections

Section

When using BacDive for research please cite our paper

Virgibacillus salarius SA-Vb 1 is a moderately halophilic, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from salt crust from Gharsa salt lake.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
mesophilic
moderately halophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Virgibacillus*
	Species	**Virgibacillus salarius**
	Full Scientific Name (LPSN)	Virgibacillus salarius Hua et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #23052]	Gram stain	positive

[Ref.: #23052]	Cell length	1.8-3.5 µm

[Ref.: #23052]	Cell width	0.6-0.9 µm

[Ref.: #23052]	Cell shape	rod-shaped

[Ref.: #23052]	Motility	yes

[Ref.: #23052]	Colony size	2.0-2.5 mm
[Ref.: #23052]	Colony color	white
[Ref.: #23052]	Colony shape	circular
[Ref.: #23052]	Incubation period	2 days
[Ref.: #23052]	Cultivation medium used	solid medium containing 10 % NaCl

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7472]

Culture medium

HP 101 HALOPHILE MEDIUM (DSMZ Medium 625)

[Ref.: #7472]

Culture medium growth

[Ref.: #7472]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7472]

Culture medium composition

expand / minimize

[Ref.: #23052]

Culture medium

solid medium containing 10 % NaCl

[Ref.: #23052]

Culture medium growth

		Temperature
[Ref.: #7472]	growth	30 °C
[Ref.: #23052]	growth	10.0-50.0 °C
[Ref.: #23052]	optimum	30.0-35.0 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #23052]			optimum	7.5
[Ref.: #23052]			growth	5.5-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23052]	Spore description	spherical or ellipsoidal,subterminal or terminal position,in swollen sporangia
[Ref.: #23052]	Type of spore	endospore
[Ref.: #23052]	Ability of spore formation	yes

[Ref.: #23052]

Halophily / tolerance level

moderately halophilic

	Salt	Tested relation		Salt conc.
[Ref.: #23052]	NaCl		growth	0.5	%
[Ref.: #23052]	NaCl	no	growth	0.0	%
[Ref.: #23052]	NaCl		optimum	7.0-10.0	%(w/v)
[Ref.: #23052]	NaCl		growth	0.5-25.0	%
[Ref.: #23052]	NaCl		growth	25.0	%

[Ref.: #23052]	Murein short key	A31
[Ref.: #23052]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23052]	2-oxobutanoate ChEBI	+	carbon source
[Ref.: #23052]	2-oxoglutarate ChEBI	+	carbon source
[Ref.: #23052]	arbutin ChEBI	+	builds acid from
[Ref.: #23052]	casein	+	hydrolysis
[Ref.: #23052]	cellobiose ChEBI	+	builds acid from
[Ref.: #23052]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23052]	D-fructose ChEBI	+	builds acid from
[Ref.: #23052]	D-fructose ChEBI	+	carbon source
[Ref.: #23052]	D-galactose ChEBI	-	builds acid from
[Ref.: #23052]	D-glucose ChEBI	+	builds acid from
[Ref.: #23052]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23052]	D-mannose ChEBI	+	builds acid from
[Ref.: #23052]	D-mannose ChEBI	+	carbon source
[Ref.: #23052]	D-tagatose ChEBI	+	builds acid from
[Ref.: #23052]	D-xylose ChEBI	-	builds acid from
[Ref.: #23052]	esculin ChEBI	+	hydrolysis
[Ref.: #23052]	gentiobiose ChEBI	+	carbon source
[Ref.: #23052]	glycerol 3-phosphate ChEBI	+	builds acid from
[Ref.: #23052]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23052]	L-fucose ChEBI	-	builds acid from
[Ref.: #23052]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23052]	L-xylose ChEBI	-	builds acid from
[Ref.: #23052]	maltose ChEBI	+	builds acid from
[Ref.: #23052]	melibiose ChEBI	-	builds acid from
[Ref.: #23052]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23052]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #23052]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #23052]	nitrate ChEBI	-	reduction
[Ref.: #23052]	salicin ChEBI	+	builds acid from
[Ref.: #23052]	starch ChEBI	-	hydrolysis
[Ref.: #23052]	sucrose ChEBI	-	builds acid from
[Ref.: #23052]	thymidine ChEBI	+	carbon source
[Ref.: #23052]	trehalose ChEBI	-	builds acid from
[Ref.: #23052]	turanose ChEBI	-	builds acid from
[Ref.: #23052]	tween 40 ChEBI	+	carbon source
[Ref.: #23052]	tween 40 ChEBI	+	hydrolysis
[Ref.: #23052]	tween 80 ChEBI	+	carbon source
[Ref.: #23052]	tween 80 ChEBI	+	hydrolysis
[Ref.: #23052]	uridine ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23052]		hydrogen sulfide ChEBI	no
[Ref.: #23052]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #23052]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23052]	catalase	+	1.11.1.6
[Ref.: #23052]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23052]	gelatinase	+
[Ref.: #23052]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #23052]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23052]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7472]	Sample type/isolated from	salt crust from Gharsa salt lake
[Ref.: #7472]	Geographic location (country and/or sea, region)	Chott el Gharsa, Sahara
[Ref.: #7472]	Country	Tunisia
[Ref.: #7472]	Country ISO 3 Code	TUN
[Ref.: #7472]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Salt-crust sample collected from Gharsa salt lake (Chott el Gharsa)
[Ref.: #67770]	Country	Tunisia
[Ref.: #67770]	Country ISO 3 Code	TUN
[Ref.: #67770]	Continent	Africa

[Ref.: #23052]	Enrichment culture	ATCC medium 925 HP 101
[Ref.: #23052]	Enrichment culture composition	containing: 1 % (w/v) peptone, 0.1 % yeast extract, 10 % NaCl, 0.43 % MgSO4.7H2O, 0.2 % NaNO3 , pH 7.2
[Ref.: #23052]	Enrichment culture temperature	30.0 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB197851 (>99% sequence identity) for Virgibacillus from Microbeatlas

Aquatic Samples	31
Soil Samples	22
Animal Samples	63
Plant Samples	5
Total Samples	121

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7472]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7472]	Virgibacillus salarius gene for 16S rRNA, partial sequence	AB197851	1564	GenBank ENA	447199 tax ID
[Ref.: #20218]	Virgibacillus salarius genes for 16S ribosomal RNA, tRNA-Ileu, tRNA-Ala, 23S ribosomal RNA, partial and complete sequence, strain: SA-Vb1, clone: 900	AB243780	796	GenBank ENA	447199 tax ID	*
[Ref.: #20218]	Virgibacillus salarius genes for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, strain: clone: 800	AB243781	687	GenBank ENA	447199 tax ID	*
[Ref.: #20218]	Virgibacillus salarius genes for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, strain: SA-Vb1, clone: 700	AB243782	688	GenBank ENA	447199 tax ID	*

[Ref.: #7472]

GC-content

37.3 mol%

capillary zone electrophoresis (CZE)

Availability in culture collections External links

[Ref.: #7472]

Culture collection no.

DSM 18441, JCM 12946

[Ref.: #71132]

SI-ID 404521

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Virgibacillus salarius sp. nov., a halophilic bacterium isolated from a Saharan salt lake.	Hua NP, Hamza-Chaffai A, Vreeland RH, Isoda H, Naganuma T	Int J Syst Evol Microbiol	10.1099/ijs.0.65693-0	2008	*

References

#7472

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18441

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23052

Ngoc-Phuc Hua,Amel Hamza-Chaffai,Russell H. Vreeland,Hiroko Isoda,Takeshi Naganuma: Virgibacillus salarius sp. nov., a halophilic bacterium isolated from a Saharan salt lake. IJSEM 58: 2409 - 2414 2008 ( DOI 10.1099/ijs.0.65693-0 , PubMed 18842865 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71132

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404521.1 )

* These data were automatically processed and therefore are not curated

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