Kitasatospora atroaurantiaca (DSM 41649, AS 4.1582, ATCC 51343)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	10426
  multimedia content:	DSM_41649.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_41649.jpg
  caption:	Medium 65 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
10426	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19821	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19821	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19821	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19821	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19821	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19821	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10426	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)		Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19821	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19821	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
19821	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19821	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19821	29864 ChEBI	mannitol	-
19821	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19821	16634 ChEBI	raffinose	-
19821	26546 ChEBI	rhamnose	-
19821	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19821	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68382	trypsin	-	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	cellulose degradation	100	5 of 5
66794	phenylmercury acetate degradation	100	2 of 2
66794	mannosylglycerate biosynthesis	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	aerobactin biosynthesis	100	1 of 1
66794	taurine degradation	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	biotin biosynthesis	100	4 of 4
66794	glycogen metabolism	100	5 of 5
66794	1,4-dihydroxy-6-naphthoate biosynthesis	100	6 of 6
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	methylglyoxal degradation	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	ketogluconate metabolism	100	8 of 8
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	adipate degradation	100	2 of 2
66794	starch degradation	100	10 of 10
66794	threonine metabolism	100	10 of 10
66794	folate polyglutamylation	100	1 of 1
66794	ethanol fermentation	100	2 of 2
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	acetate fermentation	100	4 of 4
66794	glycine betaine biosynthesis	100	5 of 5
66794	cardiolipin biosynthesis	100	7 of 7
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	palmitate biosynthesis	100	22 of 22
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	phenylalanine metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	proline metabolism	90.91	10 of 11
66794	Entner Doudoroff pathway	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	serine metabolism	88.89	8 of 9
66794	dTDPLrhamnose biosynthesis	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	heme metabolism	85.71	12 of 14
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	citric acid cycle	85.71	12 of 14
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	photosynthesis	85.71	12 of 14
66794	aclacinomycin biosynthesis	85.71	6 of 7
66794	vitamin B12 metabolism	85.29	29 of 34
66794	leucine metabolism	84.62	11 of 13
66794	glycolysis	82.35	14 of 17
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	metabolism of disaccharids	81.82	9 of 11
66794	gallate degradation	80	4 of 5
66794	factor 420 biosynthesis	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	alanine metabolism	79.31	23 of 29
66794	tryptophan metabolism	78.95	30 of 38
66794	purine metabolism	78.72	74 of 94
66794	d-mannose degradation	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	pyrimidine metabolism	77.78	35 of 45
66794	allantoin degradation	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	urea cycle	76.92	10 of 13
66794	histidine metabolism	75.86	22 of 29
66794	glycogen biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	cyclohexanol degradation	75	3 of 4
66794	carnitine metabolism	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	non-pathway related	73.68	28 of 38
66794	tyrosine metabolism	71.43	10 of 14
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	lipid metabolism	70.97	22 of 31
66794	arginine metabolism	70.83	17 of 24
66794	propionate fermentation	70	7 of 10
66794	myo-inositol biosynthesis	70	7 of 10
66794	4-hydroxyphenylacetate degradation	70	7 of 10
66794	isoprenoid biosynthesis	69.23	18 of 26
66794	methionine metabolism	69.23	18 of 26
66794	octane oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	degradation of aromatic, nitrogen containing compounds	66.67	8 of 12
66794	enterobactin biosynthesis	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	oxidative phosphorylation	65.93	60 of 91
66794	glutathione metabolism	64.29	9 of 14
66794	lysine metabolism	64.29	27 of 42
66794	degradation of sugar acids	64	16 of 25
66794	d-xylose degradation	63.64	7 of 11
66794	degradation of sugar alcohols	62.5	10 of 16
66794	phenylpropanoid biosynthesis	61.54	8 of 13
66794	degradation of pentoses	60.71	17 of 28
66794	3-phenylpropionate degradation	60	9 of 15
66794	glycine metabolism	60	6 of 10
66794	arachidonate biosynthesis	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	hydrogen production	60	3 of 5
66794	coenzyme M biosynthesis	60	6 of 10
66794	lipoate biosynthesis	60	3 of 5
66794	propanol degradation	57.14	4 of 7
66794	benzoyl-CoA degradation	57.14	4 of 7
66794	daunorubicin biosynthesis	55.56	5 of 9
66794	nitrate assimilation	55.56	5 of 9
66794	ascorbate metabolism	54.55	12 of 22
66794	sulfate reduction	53.85	7 of 13
66794	selenocysteine biosynthesis	50	3 of 6
66794	pantothenate biosynthesis	50	3 of 6
66794	ribulose monophosphate pathway	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	phenol degradation	50	10 of 20
66794	quinate degradation	50	1 of 2
66794	androgen and estrogen metabolism	50	8 of 16
66794	CMP-KDO biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	vitamin E metabolism	50	2 of 4
66794	degradation of hexoses	50	9 of 18
66794	grixazone biosynthesis	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	polyamine pathway	47.83	11 of 23
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	cholesterol biosynthesis	45.45	5 of 11
66794	lipid A biosynthesis	44.44	4 of 9
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	mevalonate metabolism	42.86	3 of 7
66794	carotenoid biosynthesis	40.91	9 of 22
66794	bacilysin biosynthesis	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	creatinine degradation	40	2 of 5
66794	arachidonic acid metabolism	38.89	7 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	36.36	4 of 11
66794	vitamin B6 metabolism	36.36	4 of 11
66794	methane metabolism	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	chlorophyll metabolism	27.78	5 of 18
66794	catecholamine biosynthesis	25	1 of 4
66794	lactate fermentation	25	1 of 4

API 20E

API zym

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence U93326 (>99% sequence identity) for Kitasatospora from Microbeatlas

Aquatic Samples	9
Soil Samples	781
Animal Samples	33
Plant Samples	5
Total Samples	828

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM782895v1 assembly for Kitasatospora atroaurantiaca DSM 41649	contig	GCA_007828955	285545.3	2818991273	285545	76.9
124043	ASM3952108v1 assembly for Kitasatospora atroaurantiaca JCM 3337	contig	GCA_039521085			285545	54.21

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Kitasatosporia melanogena 16S ribosomal RNA gene, partial sequence	U93326	1482		58353
20218	Kitasatosporia melanogena 16S-23S ribosomal RNA gene spacer, partial sequence	U93327	282		58353
10426	Streptomyces atroaurantiacus gene for 16S rRNA, partial sequence, strain: NBRC 14327	AB184593	1461		285545
10426	Kitasatospora atroaurantiaca strain NRRL B-24282 16S ribosomal RNA gene, partial sequence	DQ026645	1569		285545
124043	Kitasatospora atroaurantiaca strain JCM 3337 16S ribosomal RNA gene, partial sequence.	MT760451	1339		285545

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kitasatospora saccharophila sp. nov. and Kitasatospora kazusanensis sp. nov., isolated from soil and transfer of Streptomyces atroaurantiacus to the genus Kitasatospora as Kitasatospora atroaurantiaca comb. nov.	Li B, Furihata K, Kudo T, Yokota A	J Gen Appl Microbiol	10.2323/jgam.55.19	2009