Name: Virgibacillus olivae E30 8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1485

Type strain:

Species: Virgibacillus olivae

Strain Designation: E30 8, E₃₀ 8

Culture col. no.: DSM 18098, JCM 30551, LMG 23503, CECT 7163

Strain history: DSM 18098 <-- T. Quesada; Univ. of Granada, Spain; E₃₀8.

NCBI tax ID(s): 340172 (species)

SI-ID 290407

LMG 23503, DSM 18098, CECT 7163

Section

Virgibacillus olivae E30 8 is an aerobe, halotolerant, heterotroph bacterium that was isolated from Spanish green olive processing, waste water.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
halotolerant
heterotroph
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Virgibacillus*
	Species	**Virgibacillus olivae**
	Full Scientific Name (LPSN)	Virgibacillus olivae Quesada et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #23053]	Gram stain	positive

[Ref.: #23053]	Cell length	1.8 µm

[Ref.: #23053]	Cell width	0.26 µm

[Ref.: #23053]	Cell shape	rod-shaped

[Ref.: #23053]	Motility	yes

[Ref.: #23053]	Flagellum arrangement	peritrichous

[Ref.: #23053]	Colony size	0.5-2.0 mm
[Ref.: #23053]	Colony color	yellow-cream
[Ref.: #23053]	Incubation period	1 day
[Ref.: #23053]	Cultivation medium used	HM 5 % sea salts medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7392]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #7392]

Culture medium growth

[Ref.: #7392]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7392]

Culture medium composition

expand / minimize

[Ref.: #23053]

Culture medium

HM 5 % sea salts medium

[Ref.: #23053]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #7392]		growth	30 °C
[Ref.: #23053]		growth	20.0-45.0 °C
[Ref.: #23053]	no	growth	15.0 °C
[Ref.: #23053]	no	growth	50.0 °C
[Ref.: #23053]		optimum	30.0 °C
[Ref.: #67770]		growth	28 °C

[Ref.: #7392]	Temperature range	mesophilic
[Ref.: #23053]	Temperature range	psychrophilic
[Ref.: #23053]	Temperature range	thermophilic
[Ref.: #23053]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #23053]		optimum	7.0
[Ref.: #23053]		growth	4.0-8.0
[Ref.: #23053]	no	growth	10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23053]

Oxygen tolerance

aerobe

[Ref.: #23053]

Nutrition type

heterotroph

[Ref.: #23053]	Spore description	spherical or ellipsoidal,terminal or subterminal
[Ref.: #23053]	Type of spore	endospore
[Ref.: #23053]	Ability of spore formation	yes

[Ref.: #23053]

Halophily / tolerance level

halotolerant

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23053]		NaCl		growth	0.5	%
[Ref.: #23053]		NaCl		growth	20.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23053]	casein	+	hydrolysis
[Ref.: #23053]	cellobiose ChEBI	-	builds acid from
[Ref.: #23053]	cellobiose ChEBI	+	carbon source
[Ref.: #23053]	cetrimide ChEBI	-	growth
[Ref.: #23053]	citrate ChEBI	-	carbon source
[Ref.: #23053]	citrate ChEBI	-	energy source
[Ref.: #23053]	D-fructose ChEBI	+	builds acid from
[Ref.: #23053]	D-galactose ChEBI	-	builds acid from
[Ref.: #23053]	D-glucose ChEBI	-	builds acid from
[Ref.: #23053]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23053]	D-mannose ChEBI	-	builds acid from
[Ref.: #23053]	D-xylose ChEBI	-	builds acid from
[Ref.: #23053]	esculin ChEBI	+	hydrolysis
[Ref.: #23053]	gelatin ChEBI	+	hydrolysis
[Ref.: #23053]	glucose ChEBI	-	fermentation
[Ref.: #23053]	glucose ChEBI	+	carbon source
[Ref.: #23053]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23053]	mannose ChEBI	+	carbon source
[Ref.: #23053]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23053]	nitrate ChEBI	+	reduction
[Ref.: #23053]	nitrite ChEBI	+	reduction
[Ref.: #23053]	rhamnose ChEBI	+	carbon source
[Ref.: #23053]	ribitol ChEBI	-	builds acid from
[Ref.: #23053]	starch ChEBI	+	hydrolysis
[Ref.: #23053]	sucrose ChEBI	-	builds acid from
[Ref.: #23053]	tryptophan ChEBI	-	energy source
[Ref.: #23053]	tween 20 ChEBI	-	hydrolysis
[Ref.: #23053]	tween 40 ChEBI	-	hydrolysis
[Ref.: #23053]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23053]	tyrosine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23053]	amikacin ChEBI		yes
[Ref.: #23053]	ampicillin ChEBI	yes
[Ref.: #23053]	cefalotin ChEBI	yes
[Ref.: #23053]	clindamycin ChEBI		yes
[Ref.: #23053]	erythromycin ChEBI	yes
[Ref.: #23053]	gentamicin ChEBI	yes
[Ref.: #23053]	kanamycin ChEBI		yes
[Ref.: #23053]	nalidixic acid ChEBI		yes
[Ref.: #23053]	penicillin ChEBI	yes
[Ref.: #23053]	polymyxin b ChEBI		yes
[Ref.: #23053]	rifampicin ChEBI	yes
[Ref.: #23053]	streptomycin ChEBI		yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23053]		acetoin ChEBI	no
[Ref.: #23053]		indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #23053]	acetoin ChEBI	-
[Ref.: #23053]	glucose ChEBI		-
[Ref.: #23053]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #23053]	catalase	+	1.11.1.6
[Ref.: #23053]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23053]	tryptophan deaminase	-	4.1.99.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7392]	Sample type/isolated from	Spanish green olive processing, waste water
[Ref.: #7392]	Geographic location (country and/or sea, region)	La Roda, Sevilla
[Ref.: #7392]	Country	Spain
[Ref.: #7392]	Country ISO 3 Code	ESP
[Ref.: #7392]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Wastewater of Spanish green olive processing
[Ref.: #67770]	Geographic location (country and/or sea, region)	La Roda de Andalucia
[Ref.: #67770]	Country	Spain
[Ref.: #67770]	Country ISO 3 Code	ESP
[Ref.: #67770]	Continent	Europe

[Ref.: #23053]	Enrichment culture	HM medium
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	-
#Engineered	#Waste	#Wastewater
#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Fruit (Seed)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ139839 (>99% sequence identity) for Virgibacillus from Microbeatlas

Aquatic Samples	31
Soil Samples	22
Animal Samples	63
Plant Samples	5
Total Samples	121

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7392]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7392]	Virgibacillus olivae strain E308 16S ribosomal RNA gene, partial sequence	DQ139839	1564	ENA	340172 tax ID

[Ref.: #7392]	GC-content	33.4 mol%
[Ref.: #67770]	GC-content	33.4 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #7392]

Culture collection no.

DSM 18098, JCM 30551, LMG 23503, CECT 7163

[Ref.: #71131]

SI-ID 290407

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Virgibacillus kapii sp. nov., isolated from Thai shrimp paste (Ka-pi).	Daroonpunt R, Tanasupawat S, Kudo T, Ohkuma M, Itoh T	Int J Syst Evol Microbiol	10.1099/ijsem.0.000951	2016	*
Phylogeny	Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives.	Quesada T, Aguilera M, Morillo JA, Ramos-Cormenzana A, Monteoliva-Sanchez M	Int J Syst Evol Microbiol	10.1099/ijs.0.64550-0	2007	*

References

#7392

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18098

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23053

Teresa Quesada,Margarita Aguilera,José Antonio Morillo,Alberto Ramos-Cormenzana,Mercedes Monteoliva-Sánchez: Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives. IJSEM 57: 906 - 910 2007 ( DOI 10.1099/ijs.0.64550-0 , PubMed 17473231 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71131

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290407.1 )

* These data were automatically processed and therefore are not curated

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