Lactococcus lactis subsp. hordniae (DSM 20450, ATCC 29071, NCDO 2181)

Name: Lactococcus lactis subsp. hordniae (DSM 20450, ATCC 29071, NCDO 2181)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14693

Type strain

Strain Designation HC-1

Culture col. no. DSM 20450 ATCC 29071 NCDO 2181 CCUG 32210 HC-1 10 more

NCBI tax ID(s) 1348656 203404

Special Collections DSMZ CCUG JCM KCTC CIP

History <- R.E. Kunkee <- B.A. Latorre-Guzman, HC-1 (Lactobacillus hordniae) T. Mitsuoka S8-66 <-- B. A. Latorre-Guzman HC-1. CIP <- 1987, NCFB <- 1978, M.E. Sharpe, Reading, UK <- 1978, C.I. Kado: strain HC-1-1, Lactobacillus hordniae

Links

Lactococcus lactis subsp. hordniae HC-1 is a mesophilic prokaryote that was isolated from leafhopper .

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 92327

LMG 8520,ATCC 29071,CCUG 32210,DSM 20450,NCFB 2181,NCDO 2181,CIP 102973,JCM 1180,NCIMB 702181,CCM 4541,KCTC 3768,NBRC 100931,HAMBI 1590,VTT E-90394

@ref 20215

Last LPSN update 2025-12-16 09:50:25

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Streptococcaceae

Genus Lactococcus

Species Lactococcus lactis subsp. hordniae

Full scientific name Lactococcus lactis subsp. hordniae (ex Latorre-Guzman et al. 1977) Schleifer et al. 1986

BacDive ID	Other strains from **Lactococcus lactis subsp. hordniae** (1)	Type strain
137805	L. lactis subsp. hordniae CIP 102974, NCDO 2182, JCM 11040

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		93
125438		94.656	positive

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8822	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38744	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120526	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8822	positive	growth	30	mesophilic
38744	positive	growth	30	mesophilic
50900	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.7

Murein

@ref	Murein short key	Type
8822	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68381	29016 ChEBI	arginine	+	hydrolysis	from API rID32STR
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68381	16988 ChEBI	D-ribose	-	builds acid from	from API rID32STR
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68381	17716 ChEBI	lactose	-	builds acid from	from API rID32STR
68381	17306 ChEBI	maltose	-	builds acid from	from API rID32STR
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68381	28053 ChEBI	melibiose	-	builds acid from	from API rID32STR
68381	320055 ChEBI	methyl beta-D-glucopyranoside	-	builds acid from	from API rID32STR
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	-	builds acid from	from API rID32STR
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
68381	27082 ChEBI	trehalose	+	builds acid from	from API rID32STR
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68381	15688 ChEBI	acetoin		from API rID32STR

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68381	15688 ChEBI	acetoin	+	from API rID32STR

Enzymes

@ref	Value	Activity	Ec
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
68381	alkaline phosphatase	-	3.1.3.1	from API rID32STR
68381	alpha-galactosidase	-	3.2.1.22	from API rID32STR
68381	arginine dihydrolase	+	3.5.3.6	from API rID32STR
68381	beta-galactosidase	-	3.2.1.23	from API rID32STR
68381	beta-glucosidase	+	3.2.1.21	from API rID32STR
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
68381	glycyl tryptophan arylamidase	-		from API rID32STR
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
68381	urease	-	3.5.1.5	from API rID32STR

Fatty acid profile

Metadata FA analysis

@ref

50900

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	55.3	17.824
C16:0	22.1	16
C18:2 ω6,9c/C18:0 ANTE	9.6	17.724
C18:1 ω9c	4.5	17.769
C14:0	3.2	14
C18:0	2.4	18
C16:1 ω7c	1.6	15.819
C16:1 ω7c/C15:0 ISO 2OH	1.2	15.85

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
8822	+	+	-	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Host species
8822	leafhopper (Hordnia circellata)					Hordnia circellata
50900	Leaf hopper
67770	Leaf hopper (Hordnia circellata)					Hordnia circellata
120526	Other, Leafhopper	California	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence LC065038 (>99% sequence identity) for Lactococcus from Microbeatlas

Aquatic Samples	13841
Soil Samples	7135
Animal Samples	132033
Plant Samples	4677
Total Samples	157686

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8822	1	Risk group (German classification) According to TRBA 466
120526	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM159172v1 assembly for Lactococcus lactis subsp. hordniae NBRC 100931	contig	GCA_001591725	1348656.3	2731957664	1348656	62.01
67770	ASM869294v1 assembly for Lactococcus lactis subsp. hordniae CCUG 32210T	contig	GCA_008692945	203404.5	2906048348	203404	61.94
66792	ASM244178v1 assembly for Lactococcus lactis subsp. hordniae DSM 20450	contig	GCA_002441785	203404.4	2869442540	203404	56.36
124043	ASM4265076v1 assembly for Lactococcus lactis subsp. hordniae JCM 1180	contig	GCA_042650765			203404	54.97
66792	ASM145662v1 assembly for Lactococcus lactis subsp. lactis LMG8520	contig	GCA_001456625	1360.106	2671181044	1360	49.78
124043	ASM3953501v1 assembly for Lactococcus lactis subsp. hordniae JCM 1180	contig	GCA_039535015			203404	48.6
124043	ASM4266208v1 assembly for Lactococcus lactis subsp. hordniae JCM 1180	contig	GCA_042662085			203404	30.82

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactococcus lactis subsp. hordniae strain KCTC 3768 16S ribosomal RNA gene, partial sequence	EF694031	1444	203404
20218	Lactococcus lactis subsp. lactis strain KCTC 3768 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	HM241926	515	1360
20218	Lactococcus lactis subsp. hordniae gene for 16S rRNA, partial sequence, strain: NBRC 100931	AB681293	1472	203404
20218	Lactococcus lactis subsp. hordniae gene for 16S rRNA, partial sequence, strain: NCDO 2181T	AB100804	1499	203404
67770	Lactococcus lactis subsp. hordniae gene for 16S ribosomal RNA, partial sequence, strain: JCM 1180	LC065038	1456	203404
124043	Lactococcus lactis subsp. hordniae strain JCM 1180 16S ribosomal RNA gene, partial sequence.	MT760343	1364	203404

GC content

@ref	GC-content (mol%)
8822	35.2
8822	32.8
67770	31.9

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactococcus lactis subsp. lactis LMG8520
NCBI: GCA_001456625

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	78.50	no
125439	motility	BacteriaNetⓘ	no	54.60	no
125439	gram_stain	BacteriaNetⓘ	positive	66.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactococcus lactis subsp. hordniae NBRC 100931
PATRIC: 1348656.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	94.66	no
125438	anaerobic	anaerobicⓘ	no	90.49	no
125438	spore-forming	spore-formingⓘ	no	85.19	no
125438	aerobic	aerobicⓘ	no	96.02	no
125438	thermophilic	thermophileⓘ	no	99.00	yes
125438	flagellated	motile2+ⓘ	no	93.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome-level comparisons provide insight into the phylogeny and metabolic diversity of species within the genus Lactococcus.	Yu J, Song Y, Ren Y, Qing Y, Liu W, Sun Z.	BMC Microbiol	10.1186/s12866-017-1120-5	2017
	Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae.	Hall C, Brachat S, Dietrich FS.	Eukaryot Cell	10.1128/ec.4.6.1102-1115.2005	2005
Phylogeny	Phenotypic and genotypic characterization of some lactic Acid bacteria isolated from bee pollen: a preliminary study.	Belhadj H, Harzallah D, Bouamra D, Khennouf S, Dahamna S, Ghadbane M.	Biosci Microbiota Food Health	10.12938/bmfh.33.11	2014
Metabolism	A 7-base-pair sequence protects DNA from exonucleolytic degradation in Lactococcus lactis.	Biswas I, Maguin E, Ehrlich SD, Gruss A.	Proc Natl Acad Sci U S A	10.1073/pnas.92.6.2244	1995
	alpha-Lipoic acid increases phagocytosis of some lactic acid bacteria via modulation of CD36 expression.	Nomura N, Miyadai N, Kawase I.	Biosci Microbiota Food Health	10.12938/bmfh.2024-019	2025
Metabolism	[Continuous production of nisin by calcium alginate-immobilized Lactococcus lactis subsp. lacits SM526].	Kong J, Zhuang X, Ma G.	Wei Sheng Wu Xue Bao		2001
Genetics	Genome-scale diversity and niche adaptation analysis of Lactococcus lactis by comparative genome hybridization using multi-strain arrays.	Siezen RJ, Bayjanov JR, Felis GE, van der Sijde MR, Starrenburg M, Molenaar D, Wels M, van Hijum SA, van Hylckama Vlieg JE.	Microb Biotechnol	10.1111/j.1751-7915.2011.00247.x	2011
Enzymology	Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes.	Klijn N, Weerkamp AH, de Vos WM.	Appl Environ Microbiol	10.1128/aem.57.11.3390-3393.1991	1991
	Complete genome of Lactococcus lactis isolate S11-599 from the brain of a silver carp (Hypophthalmichthys molitrix) during a mass mortality event in the Mississippi river.	Huda N-U, Older CE, Ware C, Heckman TI, Rose D, Marancik DP, Khoo LH, Griffin MJ.	Microbiol Resour Announc	10.1128/mra.00503-25	2025
	Lactococcus lactis HkyuLL 10 suppresses colorectal tumourigenesis and restores gut microbiota through its generated alpha-mannosidase.	Su ACY, Ding X, Lau HCH, Kang X, Li Q, Wang X, Liu Y, Jiang L, Lu Y, Liu W, Ding Y, Cheung AH, To KF, Yu J.	Gut	10.1136/gutjnl-2023-330835	2024
	Succession of the microbial community during the process of mechanical and biological pretreatment coupled with a bio-filter for removal of VOCs derived from domestic waste: a field study.	Hou J, Yu C, Meng F, He X, Wang Y, Chen W, Li M.	RSC Adv	10.1039/d1ra05962a	2021
Genetics	From Genome to Phenotype: An Integrative Approach to Evaluate the Biodiversity of Lactococcus lactis.	Laroute V, Tormo H, Couderc C, Mercier-Bonin M, Le Bourgeois P, Cocaign-Bousquet M, Daveran-Mingot ML.	Microorganisms	10.3390/microorganisms5020027	2017
Genetics	Bacteriocin Antagonistic Potentials of Lactococcus cremoris and Lactococcus lactis Isolates from Different Habitats.	Tang T, Leisner JJ.	Probiotics Antimicrob Proteins	10.1007/s12602-024-10361-w	2025
Genetics	Complete Genome Insights into Lactococcus petauri CF11 Isolated from a Healthy Human Gut Using Second- and Third-Generation Sequencing.	Ou YJ, Ren QQ, Fang ST, Wu JG, Jiang YX, Chen YR, Zhong Y, Wang DD, Zhang GX.	Front Genet	10.3389/fgene.2020.00119	2020
Genetics	16S-23S rRNA Internal Transcribed Spacer Region (ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri.	Stoppani N, Colussi S, Pastorino P, Prearo M, Sciuto S, Altinok I, Ozturk RC, Ture M, Vela AI, Blanco MDM, Kotzamanidis C, Bitchava K, Malousi A, Fariano L, Volpatti D, Acutis PL, Fernandez-Garayzabal JF.	Microorganisms	10.3390/microorganisms11051320	2023
Metabolism	Spherical lactic acid bacteria activate plasmacytoid dendritic cells immunomodulatory function via TLR9-dependent crosstalk with myeloid dendritic cells.	Jounai K, Ikado K, Sugimura T, Ano Y, Braun J, Fujiwara D.	PLoS One	10.1371/journal.pone.0032588	2012
Phylogeny	Lactococcus lactis subsp. tructae subsp. nov. isolated from the intestinal mucus of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss).	Perez T, Balcazar JL, Peix A, Valverde A, Velazquez E, de Blas I, Ruiz-Zarzuela I.	Int J Syst Evol Microbiol	10.1099/ijs.0.023945-0	2011
Phylogeny	Lactococcus allomyrinae sp. nov., isolated from gut of larvae of Allomyrina dichotoma.	Heo J, Cho H, Tamura T, Saitou S, Park K, Kim JS, Hong SB, Kwon SW, Kim SJ.	Int J Syst Evol Microbiol	10.1099/ijsem.0.003461	2019
Phylogeny	Lactococcus kimchii sp. nov., a new lactic acid bacterium isolated from kimchi.	Pheng S, Han HL, Park DS, Chung CH, Kim SG	Int J Syst Evol Microbiol	10.1099/ijsem.0.003782	2020

References

#8822	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20450
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38744	; Curators of the CIP;
#50900	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32210
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68381	Automatically annotated from API rID32STR .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120526	Collection of Institut Pasteur ; Curators of the CIP; CIP 102973
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactococcus lactis subsp. hordniae (DSM 20450, ATCC 29071, NCDO 2181)

Name and taxonomic classification